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HPLC非手性柱手性柱联用技术和直接进样法在体内手性药物分析中的应用 被引量:3
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作者 王春祥 刘文英 《国外医学(药学分册)》 1997年第1期6-10,共5页
本文对体内手性药物分析中的 HPLC非手性柱 手性柱联用技术和直接进样法两方面进行综述。主要报道了非手性柱 手性柱切换联用模式及其流动相匹配、峰展宽现象和非手性柱 手性柱直接相连模式 ;以及叙述了柱切换直接进样 ,特殊手性固... 本文对体内手性药物分析中的 HPLC非手性柱 手性柱联用技术和直接进样法两方面进行综述。主要报道了非手性柱 手性柱切换联用模式及其流动相匹配、峰展宽现象和非手性柱 手性柱直接相连模式 ;以及叙述了柱切换直接进样 ,特殊手性固定相直接进样 ,固相衍生化直接进样。 展开更多
关键词 体内手性 药物分析 手性 手性 直接进样
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AN EXPERIMENTAL STUDY OF THE TUMOUR NECROSIS FACTOR LEVELSIN AQUEOUS HUMOR AFTER TRAUMATIC CATARACT AND INTRAOCULAR LENS IMPLANTATION 被引量:2
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作者 周朝晖 何守志 《Chinese Medical Sciences Journal》 CAS CSCD 1999年第1期64-66,共3页
Purpose. This paper studies the tumour necrosis factor (TNF) levels in aqueous humor after traumatic cataract extraction and posterior chamber (PC) intraocular lens (IOL) implantation in rabb... Purpose. This paper studies the tumour necrosis factor (TNF) levels in aqueous humor after traumatic cataract extraction and posterior chamber (PC) intraocular lens (IOL) implantation in rabbits,and discusses the effect of TNF on postoperative anterior ocular inflammation. Methods. Twenty seven pigmented rabbits were divided into three groups: for the first group, the IOL were placed in the capsular bag after traumatic cataract extraction; for the second, the Extracapsular cataract extraction without IOL implantation; and for the third, the control group without surgical intervention. On the 1st, 3rd, 7th and 14th day postoperatively, aqueous humor samples were obtained. A modified double antibodies indirect sandwich ELISA was used to detected for the presence of TNF. The data were studied by means of analysis of variance in SAS software. Result. The TNF level was increased in aqueous humor and reached its maximum on the 1st postoperative days after traumatic cataract extraction and PC IOLs implantation, and the TNF levels are significantly higher (P<0 05) on the 1st, 3rd, 7th and 14th day postoperatively in traumatic cataract extraction and PC IOL implanted group than that in the non surgical intervention group and extracapsular cataract extraction group. Conclusions. The increase of TNF levels had a close relationship with presence of the IOL itself which induces the secretion of TNF. This suggested that TNF as the principal mediators of immunological and inflammatory responses, so that may play critical role in anterior ocular inflammative response after traumatic cataract extraction and IOL implantation. 展开更多
关键词 intraocular lens traumatic cataract extraction tumour necrosis factor (TNF)
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