目的结合转移体内迟发超敏反应检测法初步探讨外周血中CD4+CD25+FoxP3+Treg的比例对亲体肾移植受者免疫状态评估的作用。方法收集西南医院肾科门诊随访的2005-2008年间手术的亲体肾移植供者和受者各30例,进行转移体内迟发超敏反应检测(t...目的结合转移体内迟发超敏反应检测法初步探讨外周血中CD4+CD25+FoxP3+Treg的比例对亲体肾移植受者免疫状态评估的作用。方法收集西南医院肾科门诊随访的2005-2008年间手术的亲体肾移植供者和受者各30例,进行转移体内迟发超敏反应检测(trans-vivodelayed type hypersensitivity-assay,Transvivo-DTH)实验,根据实验结果将患者分为耐受组和排斥组;运用流式细胞技术检测2组中受者外周血单个核细胞(PBMCs)中CD4+CD25+FoxP3+Treg比例。结果耐受组和排斥组裸鼠掌肿胀(同时注入受者PBMCs和供者抗原的脚掌)厚度分别为(1.72±0.44)×10-2、(8.05±2.26)×10-2mm,前者明显高于后者(P<0.05),2组受者外周血中CD4+CD25+FoxP3+Treg的比例分别为(9.23±1.79)%和(1.03±1.04)%,耐受组明显高于排斥组(P<0.05)。耐受组和排斥组裸鼠脚掌皮下炎性水肿的厚度均与外周血中CD4+CD25+FoxP3+Treg的比例呈负相关(P<0.05,r分别为0.82和0.87)。结论受者外周血中CD4+CD25+FoxP3+Treg的比例能够反应受者T细胞对供者抗原发生免疫反应的程度。外周血中CD4+CD25+FoxP3+Treg的比例在发现移植受者是否已有耐受方面有一定的参考价值。展开更多
A multi-responsive D-A type compound(CYQ)based on pyrone and triphenylamine was designed and successfully synthesized. The target compound exhibited distinct aggregation-enhanced emission(AEE) effect.Solvatochromic ex...A multi-responsive D-A type compound(CYQ)based on pyrone and triphenylamine was designed and successfully synthesized. The target compound exhibited distinct aggregation-enhanced emission(AEE) effect.Solvatochromic experiment and density functional theory(DFT) indicated CYQ possessed excellent intramolecular charge transfer(ICT) ability. Besides, its mechanofluorochromic property(MFC) was found with a 37 nm redshift. Powder wide-angle X-ray diffraction(PXRD) and differential scanning calorimetry(DSC) measurements were performed to demonstrate the transformation from the crystalline to amorphous states upon grinding. Surprisingly,CYQ displayed a hypersensitive response to trace water in organic solvents with an excellent detection limit as low as 0.0096% in tetrahydrofuran(THF). Furthermore, it was found that the fluorescent intensity of CYQ declined progressively upon humidity rise, and its color change can be witnessed by naked eyes. Therefore, the relative humidity(RH) sensing strategy guarantees the AIEgen to become a colorimetric sensor under various conditions.展开更多
文摘目的结合转移体内迟发超敏反应检测法初步探讨外周血中CD4+CD25+FoxP3+Treg的比例对亲体肾移植受者免疫状态评估的作用。方法收集西南医院肾科门诊随访的2005-2008年间手术的亲体肾移植供者和受者各30例,进行转移体内迟发超敏反应检测(trans-vivodelayed type hypersensitivity-assay,Transvivo-DTH)实验,根据实验结果将患者分为耐受组和排斥组;运用流式细胞技术检测2组中受者外周血单个核细胞(PBMCs)中CD4+CD25+FoxP3+Treg比例。结果耐受组和排斥组裸鼠掌肿胀(同时注入受者PBMCs和供者抗原的脚掌)厚度分别为(1.72±0.44)×10-2、(8.05±2.26)×10-2mm,前者明显高于后者(P<0.05),2组受者外周血中CD4+CD25+FoxP3+Treg的比例分别为(9.23±1.79)%和(1.03±1.04)%,耐受组明显高于排斥组(P<0.05)。耐受组和排斥组裸鼠脚掌皮下炎性水肿的厚度均与外周血中CD4+CD25+FoxP3+Treg的比例呈负相关(P<0.05,r分别为0.82和0.87)。结论受者外周血中CD4+CD25+FoxP3+Treg的比例能够反应受者T细胞对供者抗原发生免疫反应的程度。外周血中CD4+CD25+FoxP3+Treg的比例在发现移植受者是否已有耐受方面有一定的参考价值。
基金financial support from the National Natural Science Foundation of China (21576194)
文摘A multi-responsive D-A type compound(CYQ)based on pyrone and triphenylamine was designed and successfully synthesized. The target compound exhibited distinct aggregation-enhanced emission(AEE) effect.Solvatochromic experiment and density functional theory(DFT) indicated CYQ possessed excellent intramolecular charge transfer(ICT) ability. Besides, its mechanofluorochromic property(MFC) was found with a 37 nm redshift. Powder wide-angle X-ray diffraction(PXRD) and differential scanning calorimetry(DSC) measurements were performed to demonstrate the transformation from the crystalline to amorphous states upon grinding. Surprisingly,CYQ displayed a hypersensitive response to trace water in organic solvents with an excellent detection limit as low as 0.0096% in tetrahydrofuran(THF). Furthermore, it was found that the fluorescent intensity of CYQ declined progressively upon humidity rise, and its color change can be witnessed by naked eyes. Therefore, the relative humidity(RH) sensing strategy guarantees the AIEgen to become a colorimetric sensor under various conditions.
