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药盒法制备^(99m)Tc-RBC的人体内稳定性及内照射剂量研究
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作者 杜延荣 陈方 +2 位作者 李方 陈黎波 龙明清 《中华放射医学与防护杂志》 CAS CSCD 北大核心 2005年第5期456-458,共3页
目的研究用自制RBC药盒制备的99mTcRBC人体内稳定性和内照射辐射吸收剂量。方法4名健康志愿者,分别取肘静脉血1ml,用自制的RBC药盒进行放射性锝标记,每人注射481~555MBq,给药后5min,1,2.5,4,8和24h进行全身显像,测定99mTcRBC在人体内... 目的研究用自制RBC药盒制备的99mTcRBC人体内稳定性和内照射辐射吸收剂量。方法4名健康志愿者,分别取肘静脉血1ml,用自制的RBC药盒进行放射性锝标记,每人注射481~555MBq,给药后5min,1,2.5,4,8和24h进行全身显像,测定99mTcRBC在人体内的生物分布;计算各器官在各时间点的放射性占注入量的百分数(%ID);两种方法绘制各脏器时间放射性计数曲线,计算各脏器的滞留时间,用MIRDOSE3.0软件计算出各受照器官的内照射辐射剂量。结果99mTcRBC在人体内较稳定,血池及大血管于给药后5min至8h均可清晰显影;人体内照射辐射吸收剂量估算结果与文献值相近,按一次给药555MBq计算,各器官的吸收剂量均低于50mGy。结论用本实验室自制RBC药盒制备的99mTcRBC体内稳定性好,内照射辐射吸收剂量符合要求。 展开更多
关键词 药盒法 制备工艺 ^99MTC RBC药盒 体内稳定性 内照射剂量 吸收剂量
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灯盏乙素和苷元的理化性质及体内外稳定性研究现状 被引量:2
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作者 张培培 李德光 +1 位作者 刘红斌 唐当柱 《云南中医中药杂志》 2019年第10期77-80,共4页
通过回溯灯盏乙素和苷元的理化性质研究分析对生物利用度的影响及灯盏乙素的人体血浆、体外稳定性条件,认为灯盏乙素溶解性好,但膜通透性差,不利于吸收,而苷元溶解性差,但脂溶性强,膜通透性好,对吸收有利,提示在增加溶解性基础上,以灯... 通过回溯灯盏乙素和苷元的理化性质研究分析对生物利用度的影响及灯盏乙素的人体血浆、体外稳定性条件,认为灯盏乙素溶解性好,但膜通透性差,不利于吸收,而苷元溶解性差,但脂溶性强,膜通透性好,对吸收有利,提示在增加溶解性基础上,以灯盏乙素苷元为对象进行新药开发更有前景。为了提高灯盏乙素和苷元的体内外稳定性,需调pH为2~3,辅加Vc,尽量避免长时间与空气接触。 展开更多
关键词 灯盏乙素 苷元 理化性质 体内稳定性
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带发夹茎环结构反义核酸的体外稳定性研究
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作者 黄爱琼 姚志强 +2 位作者 周永兴 时德仁 刘志红 《实用肝脏病杂志》 CAS 1997年第4期217-217,共1页
反义寡核苷酸(antisense oligodeoxynu-cleotide,asON)在体外进行抗病毒基因复制与表达研究国内外已有多篇报道,但其应用于体内主要障碍之一是易被血清中各种核酸酶降解,因此,成药性修饰成为其临床应用前景的关键,核酸酶降解未修饰或硫... 反义寡核苷酸(antisense oligodeoxynu-cleotide,asON)在体外进行抗病毒基因复制与表达研究国内外已有多篇报道,但其应用于体内主要障碍之一是易被血清中各种核酸酶降解,因此,成药性修饰成为其临床应用前景的关键,核酸酶降解未修饰或硫代磷酸化修饰的asON一般是从3′端逐渐外切(Antisense Re- 展开更多
关键词 反义核酸 茎环结构 体外稳定性 反义寡核苷酸 酶降解 西安市 第四军医大学 传染科 体内稳定性 发夹
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装载siRNA的纳米阳离子脂质体在小鼠体内的药代动力学研究 被引量:1
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作者 问天娇 陈欣然 +4 位作者 白靖 郑颖 王雅鹃 于佩佩 崔京霞 《癌变.畸变.突变》 CAS 2022年第6期445-448,462,共5页
目的:探讨siRNA药物的体内药代动力学评价方法,并分析脂质体作为药物载体的优势。方法:采用前期已构建的siRNA表达质粒及装载了该质粒的纳米隐形阳离子脂质体,将实验分为裸siRNA质粒组和siRNA质粒脂质体组,分别进行DNaseⅠ酶切和血清中... 目的:探讨siRNA药物的体内药代动力学评价方法,并分析脂质体作为药物载体的优势。方法:采用前期已构建的siRNA表达质粒及装载了该质粒的纳米隐形阳离子脂质体,将实验分为裸siRNA质粒组和siRNA质粒脂质体组,分别进行DNaseⅠ酶切和血清中稳定性的检测。再将20μg siRNA裸质粒及siRNA质粒脂质体通过尾静脉注射进入小鼠体内,分别在注射后0 min、5 min、15 min、30 min、1 h、2 h、4 h、12 h、24 h取血,采用实时荧光定量PCR方法(qPCR)对小鼠体内裸质粒及质粒脂质体进行定量检测,计算各时间点siRNA质粒的浓度。结果:裸质粒在DNaseⅠ中仅20 min就全部降解,质粒脂质体降解较缓慢,到24 h仍有(20.16±2.47)%的DNA残留;裸质粒在80%血清中20 min时仅残留(11.03±0.92)%,在1 h时基本全部降解,质粒脂质体在80%血清中24 h时仍残留(10.26±1.04)%,与裸质粒组相比,脂质体作为载体使siRNA表达质粒在DNaseⅠ及血清中的稳定性显著提高(P<0.05)。在小鼠体内脂质体包裹的质粒半衰期约为2 h,而裸质粒仅为6 min,差异具有统计学意义(P<0.05)。结论:利用qPCR方法可以完成siRNA脂质体的体内检测。阳离子脂质体能够保护核酸类药物进入体内,是一种高效的递送载体,同时脂质体能够提高药物的体内稳定性,延长药物的作用时间。 展开更多
关键词 小干扰RNA 阳离子脂质体 体内稳定性 核酸定量检测 药代动力学
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Stability of Cereal Crops to Drought and Saline Stress in Vivo and in Vitro
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作者 Nina Terletskaya Nina Khailenko Kabl Zhambakin 《Journal of Life Sciences》 2013年第2期135-144,共10页
Complex research is devoted to basic non-specific stress-reactions caused by abiotic factors such as drought and salinity in vivo and in vitro. A comparative physiological, biochemical and cytogenetic analysis was per... Complex research is devoted to basic non-specific stress-reactions caused by abiotic factors such as drought and salinity in vivo and in vitro. A comparative physiological, biochemical and cytogenetic analysis was performed and showed the peculiarities of growth and viability on various (cellular, tissular, organismic) levels of plants structural arrangement at stress conditions. Determined the parameters of the growth, ion balance, the content of free proline, superoxide dismutase activity and conducted the cytological studies. The commonness of cytological reactions of plant cells to abiotic stress was revealed. The considerable positive correlation relationships between growth of callus biomass and increases of primary roots number under abiotic stressess, between growth of callus biomass and capacity for survival of seedlings under osmotic stress were registered. Such correlation tells about comparability of stress tolerance valuation at different levels of plants structural arrangement. The considerable negative correlation between K~/Na~ ions relations and percent increase of free proline in calluses were showed. Physiological and biochemical indicators of abiotic stresses impact on plants cells and tissues, such as SOD activity and K^+/Na^+ ions correlation were noted. These indicators are effective as metabolic markers in the course of testing and selection of stress-resistant cereals in vivo and in vitro. 展开更多
关键词 CEREALS osmotic stress salt stress in vivo in vitro.
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Prediction of fish body's passive visco-elastic properties and related muscle mechanical performance in vivo during steady swimming 被引量:2
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作者 ZHANG Wei YU YongLiang TONG BingGang 《Science China(Physics,Mechanics & Astronomy)》 SCIE EI CAS 2014年第2期354-364,共11页
For attaining the optimized locomotory performance of swimming fishes,both the passive visco-elastic properties of the fish body and the mechanical behavior of the active muscles should coordinate with the fish body’... For attaining the optimized locomotory performance of swimming fishes,both the passive visco-elastic properties of the fish body and the mechanical behavior of the active muscles should coordinate with the fish body’s undulatory motion pattern.However,it is difficult to directly measure the visco-elastic constitutive relation and the muscular mechanical performance in vivo.In the present paper,a new approach based on the continuous beam model for steady swimming fish is proposed to predict the fish body’s visco-elastic properties and the related muscle mechanical behavior in vivo.Given the lateral travelling-wave-like movement as the input condition,the required muscle force and the energy consumption are functions of the fish body’s visco-elastic parameters,i.e.the Young’s modulus E and the viscosity coefficient in the Kelvin model.After investigating the variations of the propagating speed of the required muscle force with the fish body’s visco-elastic parameters,we analyze the impacts of the visco-elastic properties on the energy efficiencies,including the energy utilization ratios of each element of the kinematic chain in fish swimming and the overall efficiency.Under the constraints of reasonable wave speed of muscle activation and the physiological feasibility,the optimal design of the passive visco-elastic properties can be predicted aiming at maximizing the overall efficiency.The analysis is based on the small-amplitude steady swimming of the carangiform swimmer,with typical Reynolds number varying from 2.5×104to 2.5×105,and the present results show that the non-dimensional Young’s modulus is 112±34,and the non-dimensional viscosity coefficient is 13 approximately.In the present estimated ranges,the overall efficiency of the swimming fish is insensitive to the viscosity,and its magnitude is about 0.11±0.02,in the predicted range given by previous study. 展开更多
关键词 fish swimming visco-elastic properties muscle mechanics efficiency
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