拨云锭眼药对9种常见致病菌的体外抗菌作用观察

目的 观察拨云锭对9种常见致病菌的体外抗菌作用。方法 采用试管稀释,用无菌肉汤培养基将拨云锭药物原液,从1/5～1/2560等倍稀释,种入菌液,置37℃孵育18～24h,观察抑菌效果;选取药液接种于普通培养基,培养18～24h后观察杀菌效果。结果 拨云锭对金黄色葡萄球菌、白色葡萄球菌、甲型链球菌。乙型链球菌抑菌活力较强,对大肠杆菌、枯草杆菌有一定抑菌作用,对绿脓杆菌、痢疾杆菌。变形杆菌抑菌作用较弱。拨云锭在高浓度时对金黄色葡萄球菌,白色葡萄球菌,甲、乙型链球菌呈现杀菌作用。结论 拨云锭眼药具有广谱抗菌作用。

哌拉西林与加替沙星联用对铜绿假单胞菌的联合药敏研究

目的 评价哌拉西林与加替沙星联合用药,对30株临床分离的铜绿假单胞菌的体外抗菌效应。方法 采用棋盘法设计,微量肉汤稀释法测定。测定不同浓度组合的抗菌药物对30株临床分离的铜绿假单胞菌的最低抑菌浓度,并计算FIC指数判定联合效应。FIC≤0 .5为协同作用,0 .5 <FIC≤1为相加作用,1<FIC≤2为无关作用,FIC >2为拮抗作用。结果 哌拉西林与加替沙星联用后,其MIC50 显著降低。FIC指数分布:FIC≤0 .5占4 0 % ;0 .5 <FIC≤1占5 0 % ;1<FIC≤2占10 % ;FIC >2为0。结论 结果表明两药联用,对铜绿假单胞菌表现为协同作用和相加作用,并以协同作用为主,无关作用较少,更无拮抗作用。

Design,synthesis,and biological evaluation of novel benzimidazole derivatives and their interaction with calf thymus DNA and synergistic effects with clinical drugs

A series of new benzimidazole derivatives was synthesized and characterized by IR,1H NMR,13C NMR,MS,and HRMS spectra.All the new compounds were screened for their antimicrobial activities in vitro by a twofold serial dilution technique.The bioactive evaluation showed that 3,5-bis(trifluoromethyl)phenyl benzimidazoles were comparably or even more strongly antibacterial and antifungal than the reference drugs Chloromycin,Norfloxacin,and Fluconazole.The combination of2,4-difluorobenzyl benzimidazole derivative 5l and its hydrochloride 7 respectively with the antibacterials Chloromycin,Norfloxacin,and the antifungal Fluconazole was more sensitive to methicillin-resistant MRSA and Fluconazole-insensitive A.flavus.In addition,the interaction of compound 5l with calf thymus DNA demonstrated that this compound could effectively intercalate into DNA to form a compound 5l-DNA complex that might block DNA replication and thereby exert good antimicrobial activity.