采用高温浸提法提取酵母细胞壁中的甘露聚糖,采用Polar MC 60Q强阴离子交换柱法对粗多糖进行纯化,其纯化产物中无色素成分,蛋白含量为0.21%,甘露聚糖得率12.1%,除蛋白效果与Sevag法相当,操作更加快捷且过程中不使用有机溶剂,无环境污染...采用高温浸提法提取酵母细胞壁中的甘露聚糖,采用Polar MC 60Q强阴离子交换柱法对粗多糖进行纯化,其纯化产物中无色素成分,蛋白含量为0.21%,甘露聚糖得率12.1%,除蛋白效果与Sevag法相当,操作更加快捷且过程中不使用有机溶剂,无环境污染。比较交换柱法纯化产物YM-1与Sevag法纯化产物YM-2的理化性质发现:2种纯化产物均由葡萄糖和甘露糖组成,YM-1中甘露糖与葡萄糖的含量比R值为2.751,YM-2为7.815,提示2种纯化产物结构上可能有较大差异;继续考察纯化产物YM-1和YM-2的红外光谱性质与分子质量分布发现:纯化产物均具有多糖物质的特征峰,但YM-1与YM-2分子质量分布差异较大,交换柱对多糖结构确有影响;继续考察纯化产物的体外生物活性,发现2种产物的体外抗氧化活性相近,但YM-1的体外降血糖活性更优。研究结果证明Polar MC 60Q强阴离子交换柱法是一种切实可行的纯化方法,同时也为后续试验提供了研究方向。展开更多
Oxidative stress and diabetes have a tendency to alter protein, lipid, and DNA moieties. One of the strategic methods used to reduce diabetes-associated oxidative stress is to inhibit the carbohydrate-digesting enzyme...Oxidative stress and diabetes have a tendency to alter protein, lipid, and DNA moieties. One of the strategic methods used to reduce diabetes-associated oxidative stress is to inhibit the carbohydrate-digesting enzymes, thereby decreasing gastrointestinal glucose production. Plant-derived natural antioxidant molecules are considered a therapeutic tool in the treatment of oxidative stress and diabetes. The objective of this study was to identify tartary buckwheat rice flavonoids and evaluate the effect of in vitro digestion on their antioxidant and antidiabetic properties. High performance liquid chromatography（HPLC） analysis indicated the presence of rutin as a major component and quercitrin as a minor component of both digested and non-digested flavonoids. Both extracts showed a significant antioxidant capacity, but digested flavonoids showed reduced activity compared to non-digested. There were some decreases of the antioxidant activities（2,2＇-azino-bis-（3-ethylbenzthiazoline-6-sulfonic acid） diammonium salt（ABTS）, 2,2-diphenyl-1-picrylhydrazy（DPPH） radical, and ferric reducing antioxidant power（FRAP）） of digested tartary buckwheat rice flavonoids compared with non-digested. Flavonoids from both groups significantly inhibited reactive oxygen species（ROS） production and α-glucosidase activity. Both digested and non-digested flavonoids markedly increased glucose consumption and glycogen content in Hep G2 cells. Tartary buckwheat rice flavonoids showed appreciable antioxidant and antidiabetic properties, even after digestion. Tartary buckwheat rice appears to be a promising functional food with potent antioxidant and antidiabetic properties.展开更多
文摘采用高温浸提法提取酵母细胞壁中的甘露聚糖,采用Polar MC 60Q强阴离子交换柱法对粗多糖进行纯化,其纯化产物中无色素成分,蛋白含量为0.21%,甘露聚糖得率12.1%,除蛋白效果与Sevag法相当,操作更加快捷且过程中不使用有机溶剂,无环境污染。比较交换柱法纯化产物YM-1与Sevag法纯化产物YM-2的理化性质发现:2种纯化产物均由葡萄糖和甘露糖组成,YM-1中甘露糖与葡萄糖的含量比R值为2.751,YM-2为7.815,提示2种纯化产物结构上可能有较大差异;继续考察纯化产物YM-1和YM-2的红外光谱性质与分子质量分布发现:纯化产物均具有多糖物质的特征峰,但YM-1与YM-2分子质量分布差异较大,交换柱对多糖结构确有影响;继续考察纯化产物的体外生物活性,发现2种产物的体外抗氧化活性相近,但YM-1的体外降血糖活性更优。研究结果证明Polar MC 60Q强阴离子交换柱法是一种切实可行的纯化方法,同时也为后续试验提供了研究方向。
基金supported by the Beijing Municipal Science and Technology Plan(No.D151100004015002)the Research Foundation of the Education Department of Zhejiang Province(No.Y201328143),China
文摘Oxidative stress and diabetes have a tendency to alter protein, lipid, and DNA moieties. One of the strategic methods used to reduce diabetes-associated oxidative stress is to inhibit the carbohydrate-digesting enzymes, thereby decreasing gastrointestinal glucose production. Plant-derived natural antioxidant molecules are considered a therapeutic tool in the treatment of oxidative stress and diabetes. The objective of this study was to identify tartary buckwheat rice flavonoids and evaluate the effect of in vitro digestion on their antioxidant and antidiabetic properties. High performance liquid chromatography（HPLC） analysis indicated the presence of rutin as a major component and quercitrin as a minor component of both digested and non-digested flavonoids. Both extracts showed a significant antioxidant capacity, but digested flavonoids showed reduced activity compared to non-digested. There were some decreases of the antioxidant activities（2,2＇-azino-bis-（3-ethylbenzthiazoline-6-sulfonic acid） diammonium salt（ABTS）, 2,2-diphenyl-1-picrylhydrazy（DPPH） radical, and ferric reducing antioxidant power（FRAP）） of digested tartary buckwheat rice flavonoids compared with non-digested. Flavonoids from both groups significantly inhibited reactive oxygen species（ROS） production and α-glucosidase activity. Both digested and non-digested flavonoids markedly increased glucose consumption and glycogen content in Hep G2 cells. Tartary buckwheat rice flavonoids showed appreciable antioxidant and antidiabetic properties, even after digestion. Tartary buckwheat rice appears to be a promising functional food with potent antioxidant and antidiabetic properties.
