Objective: To inhibit specifically survivin expression and block its function in leukemia cells, an antisense RNA expression plasmid for survivin was constructed and transfected into a leukemia cell line.Methods: A cD...Objective: To inhibit specifically survivin expression and block its function in leukemia cells, an antisense RNA expression plasmid for survivin was constructed and transfected into a leukemia cell line.Methods: A cDNA fragment of survivin obtained by RT-PCR was inserted into a plasmid vector named pcDNA3 in the reverse direction. Antisense RNA of survivin was confirmed by restriction enzyme digestion and DNA sequencing. The recombinant plasmid was transfected into the cell line HL-60 by electroporation. The effect of survivin antisense RNA on survivin mRNA expression in transfected cells was examined by RT-PCR.Results: The correct construction of the recombinant plasmid has been shown by restriction enzyme digestion and DNA sequencing. As compared to controls, the level of survivin mRNA expression in transfected cells decreased significantly.Conclusion: An antisense RNA vector for survivin has been successfully constructed and may be useful as a specific inhibitor in leukemia cells. Thus, antisense therapy on the basis of survivin can be further explored in leukemia. Key words leukemia - survivin - antisense RNA This project was supported by a grant from National Key Basis Research Program of China (No. CB 513109) and the National Natural Sciences Foundation of China (No. 39970693).展开更多
For the crossed cube,an equivalent definition based on the quadrilateral is given,by which we obtain the main properties of this topological architecture,and propose a procedure to find a shortest path between any two...For the crossed cube,an equivalent definition based on the quadrilateral is given,by which we obtain the main properties of this topological architecture,and propose a procedure to find a shortest path between any two vertices in it.展开更多
In this article, author evaluated past/present perspectives about philosophy and branches of philosophy due to historical period, religious perspective, and due to their organized categories/branches or areas. Some ty...In this article, author evaluated past/present perspectives about philosophy and branches of philosophy due to historical period, religious perspective, and due to their organized categories/branches or areas. Some types of interactions between some disciplines are given as an example. The purpose of this article is, to solve problems related with philosophy and past branches of philosophy, to define new philosophy perspective in the new system, to define new questions and questioning about philosophy or branches of philosophy, to define new or re-constructed branches of philosophy, to define the relations between the philosophy branches, to define good and/or correct structure of philosophy and branches of philosophy, to extend the definition/limits of philosophy, others. Author considered R-Synthesis as a method for the evaluation of the philosophy and related past branches of philosophy. This R-Synthesis includes general/specific perspective with eight categories, 21-dimensions, and twelve general subjects (with related scope and contents) for the past 12,000 years. It is a kind of synthesis of supernaturalism and naturalism, physics and metaphysics, others. In this article, author expressed 27 possible definitive/certain result cases of the new synthesis and defined the possible formation stages to express new theories, new disciplines, theory of interaction, theory of relation, hybrid theory, and others as constructional and/or complementary theories. These theories are considered for 21 major effective disciplines which are defined for a country and for the world. New philosophy perspective, branches of philosophy, and aims/purposes of R-Philosophy are defined to organize many inquiries about the name, number, and relation between special subject "X" and "philosophy of X" in some manner. This new perspective includes necessary and sufficient number of philosophy branches, and so it limits the number of "philosophy of X" in the philosophical system. New Era Philosophy is defined with its sub branches, its constructional philosophies, and with its 8D hybrid philosophy perspective. Ideal Philosophical System is defined with general/specific figure. Some of the new and/or re-constructed branches of philosophy explained with the new defined set of questions, new sub branches and constructional philosophies. Integration of the past/present branches of philosophy into the ideal philosophical system is explained generally. Philosophical interests of the some past philosophers and their relations with the ideal philosophical system expressed with table.展开更多
The Almighty created man for contentment, and not for suffering. Creation of the universe was a leap from absolute entropy to substance and the vital world. According to our hypothesis, the goal of creating the univer...The Almighty created man for contentment, and not for suffering. Creation of the universe was a leap from absolute entropy to substance and the vital world. According to our hypothesis, the goal of creating the universe and humans was to create enjoyment and pleasure by depressing the level of entropy. Current definitions do not adequately express the nature and origin of happiness or suffering, nor do they suggest how to treat, relieve, and prevent the torment of a healthy or sick individual. Our team developed and proposed the entropy theory as a new definition for human happiness and suffering. Entropy is a lack of order or a state of chaos. Human happiness may be defined as the complex of positive sensations, perceptions, emotions, or thoughts. These arise due to the depressed level of entropy of the individual's organism, empathy for others, or germane surroundings in the past, present, or in the future. Human suffering is the complex of negative sensations, perceptions, emotions, or thoughts that arise due to an increased level of entropy of a person's organism, empathy for others, or germane surroundings in the past, present, or future. A source of happiness and pleasure denotes depression of entropy in humans. Therefore, in order to treat suffering, one should ensure that the entropy level is reduced by complementing it with that which is missing.展开更多
The fact that the security facilities within a system are closely coupled and the security facilities between systems are unconnected results in an isolated protection structure for systems, and gives rise to a seriou...The fact that the security facilities within a system are closely coupled and the security facilities between systems are unconnected results in an isolated protection structure for systems, and gives rise to a serious challenge to system security integrations and system controls. Also, the need for diversified services and flexible extensions of network security asks for more considerations and contribu?tions from the perspective of software engineering in the process of designing and constructing security systems. Based on the essence of the virtualization technique and the idea of software-defined networks, we in this paper propose a novel software-defi ned security architecture for systems. By abstracting the traditional security facilities and techniques, the proposed security architecture provides a new, simple, effective, and programmable framework in which security operations and security controls can be decoupled, and thereby reduces the software module sizes, decreases the intensity of software deve?lopments, and improves the security extensibility of systems.展开更多
There were for a long time two invariant forms of hydrodynamic equations: one was related to coordinate system of references, and the other was versus to measure units of characteristics. These both invariant forms h...There were for a long time two invariant forms of hydrodynamic equations: one was related to coordinate system of references, and the other was versus to measure units of characteristics. These both invariant forms had important roles in the development of theoretical and practical applications of hydro-aerodynamics and related industries. The third invariant form of hydrodynamic equations is one for the dimensions of spaces. For this goal, the hyper quantities (space and physics) are introduced. Then these are created we can easily cover all problems in arbitrary dimensions (3D, 2D, 1D, separate space for liquids or constituent matters). In particularly, when they are applied to water hammer problem, which is an especially problem, we can receive immediately celerity and pressure of the event.展开更多
基金This project was supported by a grant from National Key Basis Research Program of China(No.CB 513109)and the NationalNatural Sciences Foundation of China(No.39970693).
文摘Objective: To inhibit specifically survivin expression and block its function in leukemia cells, an antisense RNA expression plasmid for survivin was constructed and transfected into a leukemia cell line.Methods: A cDNA fragment of survivin obtained by RT-PCR was inserted into a plasmid vector named pcDNA3 in the reverse direction. Antisense RNA of survivin was confirmed by restriction enzyme digestion and DNA sequencing. The recombinant plasmid was transfected into the cell line HL-60 by electroporation. The effect of survivin antisense RNA on survivin mRNA expression in transfected cells was examined by RT-PCR.Results: The correct construction of the recombinant plasmid has been shown by restriction enzyme digestion and DNA sequencing. As compared to controls, the level of survivin mRNA expression in transfected cells decreased significantly.Conclusion: An antisense RNA vector for survivin has been successfully constructed and may be useful as a specific inhibitor in leukemia cells. Thus, antisense therapy on the basis of survivin can be further explored in leukemia. Key words leukemia - survivin - antisense RNA This project was supported by a grant from National Key Basis Research Program of China (No. CB 513109) and the National Natural Sciences Foundation of China (No. 39970693).
文摘For the crossed cube,an equivalent definition based on the quadrilateral is given,by which we obtain the main properties of this topological architecture,and propose a procedure to find a shortest path between any two vertices in it.
文摘In this article, author evaluated past/present perspectives about philosophy and branches of philosophy due to historical period, religious perspective, and due to their organized categories/branches or areas. Some types of interactions between some disciplines are given as an example. The purpose of this article is, to solve problems related with philosophy and past branches of philosophy, to define new philosophy perspective in the new system, to define new questions and questioning about philosophy or branches of philosophy, to define new or re-constructed branches of philosophy, to define the relations between the philosophy branches, to define good and/or correct structure of philosophy and branches of philosophy, to extend the definition/limits of philosophy, others. Author considered R-Synthesis as a method for the evaluation of the philosophy and related past branches of philosophy. This R-Synthesis includes general/specific perspective with eight categories, 21-dimensions, and twelve general subjects (with related scope and contents) for the past 12,000 years. It is a kind of synthesis of supernaturalism and naturalism, physics and metaphysics, others. In this article, author expressed 27 possible definitive/certain result cases of the new synthesis and defined the possible formation stages to express new theories, new disciplines, theory of interaction, theory of relation, hybrid theory, and others as constructional and/or complementary theories. These theories are considered for 21 major effective disciplines which are defined for a country and for the world. New philosophy perspective, branches of philosophy, and aims/purposes of R-Philosophy are defined to organize many inquiries about the name, number, and relation between special subject "X" and "philosophy of X" in some manner. This new perspective includes necessary and sufficient number of philosophy branches, and so it limits the number of "philosophy of X" in the philosophical system. New Era Philosophy is defined with its sub branches, its constructional philosophies, and with its 8D hybrid philosophy perspective. Ideal Philosophical System is defined with general/specific figure. Some of the new and/or re-constructed branches of philosophy explained with the new defined set of questions, new sub branches and constructional philosophies. Integration of the past/present branches of philosophy into the ideal philosophical system is explained generally. Philosophical interests of the some past philosophers and their relations with the ideal philosophical system expressed with table.
文摘The Almighty created man for contentment, and not for suffering. Creation of the universe was a leap from absolute entropy to substance and the vital world. According to our hypothesis, the goal of creating the universe and humans was to create enjoyment and pleasure by depressing the level of entropy. Current definitions do not adequately express the nature and origin of happiness or suffering, nor do they suggest how to treat, relieve, and prevent the torment of a healthy or sick individual. Our team developed and proposed the entropy theory as a new definition for human happiness and suffering. Entropy is a lack of order or a state of chaos. Human happiness may be defined as the complex of positive sensations, perceptions, emotions, or thoughts. These arise due to the depressed level of entropy of the individual's organism, empathy for others, or germane surroundings in the past, present, or in the future. Human suffering is the complex of negative sensations, perceptions, emotions, or thoughts that arise due to an increased level of entropy of a person's organism, empathy for others, or germane surroundings in the past, present, or future. A source of happiness and pleasure denotes depression of entropy in humans. Therefore, in order to treat suffering, one should ensure that the entropy level is reduced by complementing it with that which is missing.
基金supported in part by the following grants:National Science Foundation of China(Grant No.61272400)Chongqing Innovative Team Fund for College Development Project(Grant No.KJTD201310)+3 种基金Chongqing Youth Innovative Talent Project(Grant No.cstc2013kjrc-qnrc40004)Science and Technology Research Program of the Chongqing Municipal Education Committee(Grant No.KJ1500425)Foundation of CQUPT(Grant No.WF201403)Chongqing Graduate Research and Innovation Project(Grant No.CYS14146)
文摘The fact that the security facilities within a system are closely coupled and the security facilities between systems are unconnected results in an isolated protection structure for systems, and gives rise to a serious challenge to system security integrations and system controls. Also, the need for diversified services and flexible extensions of network security asks for more considerations and contribu?tions from the perspective of software engineering in the process of designing and constructing security systems. Based on the essence of the virtualization technique and the idea of software-defined networks, we in this paper propose a novel software-defi ned security architecture for systems. By abstracting the traditional security facilities and techniques, the proposed security architecture provides a new, simple, effective, and programmable framework in which security operations and security controls can be decoupled, and thereby reduces the software module sizes, decreases the intensity of software deve?lopments, and improves the security extensibility of systems.
文摘There were for a long time two invariant forms of hydrodynamic equations: one was related to coordinate system of references, and the other was versus to measure units of characteristics. These both invariant forms had important roles in the development of theoretical and practical applications of hydro-aerodynamics and related industries. The third invariant form of hydrodynamic equations is one for the dimensions of spaces. For this goal, the hyper quantities (space and physics) are introduced. Then these are created we can easily cover all problems in arbitrary dimensions (3D, 2D, 1D, separate space for liquids or constituent matters). In particularly, when they are applied to water hammer problem, which is an especially problem, we can receive immediately celerity and pressure of the event.