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温度和水分对不同基因型小麦未成熟胚体细胞胚胎发生以及分化能力的影响 被引量:4
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作者 刘勇刚 徐子勤 郝建国 《西北植物学报》 CAS CSCD 2001年第3期425-431,T001,共8页
对小麦未成熟胚盾片组织离体再生途径中 ,未成熟胚发育时期以及不同小麦品种的体细胞胚发生能力和体细胞胚的分化能力进行了研究。在所试的 1 4个小麦品种中 ,筛选出具有很强的体细胞胚发生能力和体细胞胚分化能力的 4个品种 :西农 1 37... 对小麦未成熟胚盾片组织离体再生途径中 ,未成熟胚发育时期以及不同小麦品种的体细胞胚发生能力和体细胞胚的分化能力进行了研究。在所试的 1 4个小麦品种中 ,筛选出具有很强的体细胞胚发生能力和体细胞胚分化能力的 4个品种 :西农 1 376、盐 2号、85+1 - 3和宝丰 72 2 8。为进一步给小麦离体遗传操作打下基础 ,研究还对温度的影响进行了分析 ,通过低温手段解决了胚性愈伤组织随继代天数的延长体细胞胚分化能力快速降低的问题。同时研究还首次分析了干燥处理对小麦体细胞胚转换能力的影响 ,建立起一套高效的小麦离体培养再生体系 ,而且该体系从接种未成熟胚到再生植株移至土壤只需 1 0~ 1 2周时间 。 展开更多
关键词 小麦 未成熟 体细胞发生 体细胞胚分化 再生 基因型 温度 水分
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不同外植体及植物生长调节剂对几种豆科牧草体细胞胚诱导的影响 被引量:10
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作者 靳慧卿 米福贵 +2 位作者 闫利军 于洁 贾振宇 《植物生理学报》 CAS CSCD 北大核心 2015年第12期2169-2174,共6页
以6个豆科牧草材料的5种不同外植体(幼根、子叶、下胚轴、茎和叶片)为试材,研究了不同生长调节剂组合下的愈伤组织诱导及体细胞胚分化情况。结果表明:基因型、外植体类型和植物生长调节剂种类及其浓度均显著影响供试材料愈伤组织的诱导... 以6个豆科牧草材料的5种不同外植体(幼根、子叶、下胚轴、茎和叶片)为试材,研究了不同生长调节剂组合下的愈伤组织诱导及体细胞胚分化情况。结果表明:基因型、外植体类型和植物生长调节剂种类及其浓度均显著影响供试材料愈伤组织的诱导与体细胞胚的分化。不同基因型以‘中苜2号’紫花苜蓿和百脉根整体表现良好,平均愈伤组织诱导率分别为86.67%和90.13%,平均体细胞胚分化率分别为36.63%和50.93%;不同外植体间愈伤组织诱导率及体细胞胚分化率差异较大,其中下胚轴是理想的受体材料,表现为出愈早,体细胞胚形成和再生能力强。供试材料愈伤组织诱导和体细胞胚分化的适宜生长调节剂组合分别为2.0 mg·L^(-1) 2,4-D+0.5 mg·L^(-1) KT和0.1 mg·L^(-1) NAA+0.5 mg·L^(-1) 6-BA。 展开更多
关键词 豆科牧草 外植体 植物生长调节剂 愈伤组织诱导 体细胞胚分化
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Promoting human embryonic stem cell renewal or differentiation by modulating Wnt signal and culture conditions 被引量:7
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作者 Liuhong Cai Zhaohui Ye +3 位作者 Betty Ying Zhou Prashant Mali Canquan Zhou Linzhao Cheng 《Cell Research》 SCIE CAS CSCD 2007年第1期62-72,共11页
We previously showed that Wnt3a could stimulate human embryonic stem (hES) cell proliferation and affect cell fate determination. In the absence of feeder cell--derived factors, hES cells cultured under a feeder-fre... We previously showed that Wnt3a could stimulate human embryonic stem (hES) cell proliferation and affect cell fate determination. In the absence of feeder cell--derived factors, hES cells cultured under a feeder-free condition survived and proliferated poorly. Adding recombinant Wnt3a in the absence of feeder cell derived-factors stimulated hES cell proliferation but also differentiation. In the present study, we further extended our analysis to other Wnt ligands such as Wntl and Wnt5a. While Wntl displayed a similar effect on hES cells as Wnt3a, Wnt5a had little effect in this system. Wnt3a and Wntl enhanced proliferation of undifferentiated hES cells when feeder-derived self-renewal factors and bFGF are also present. To explore the possibility to promote the proliferation of undifferentiated hES cells by activating the Wnt signaling, we overexpressed Wnt3a or Wntl gene in immortalized human adult fibroblast (HAFi) cells that are superior in supporting long-term growth of undifferentiated hES cells than primary mouse embryonic fibroblasts. HAFi cells with or without a Wnt tmnsgene can be propagated indefinitely. Over-expression of the Wnt3a gene significantly enhanced the ability of HAFi feeder cells to support the undifferentiated growth of 3 different hES cell lines we tested. Co-expression of three commonly-used drug selection genes in Wnt3a-overpressing HAFi cells further enabled us to select rare hES clones after stable transfection or transduction. These immortalized engineered feeder cells (W3R) that co-express growth-promoting genes such as Wnt3a and three drug selection genes should empower us to efficiently make genetic modified hES cell lines for basic and translational research. 展开更多
关键词 WN human embryonic stem cells stem cell renewal stem cell differentiation TRANSGENE
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Differentiation of mouse embryonic stem cells into insulin-secreting cells in vitro 被引量:1
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作者 Sui Jing Jiang Fangxu Shi Bingyin 《Journal of Medical Colleges of PLA(China)》 CAS 2011年第1期1-12,共12页
Regenerative medicine, including cell-replacement strategies, may have an important role in the treatment of type 1 diabetes which is associated with decreased islet cell mass. To date, significant progress has been m... Regenerative medicine, including cell-replacement strategies, may have an important role in the treatment of type 1 diabetes which is associated with decreased islet cell mass. To date, significant progress has been made in generating insulin-secreting 13 cells from pluripotent mouse embryonic stem cells (ESCs).The aim of this study is to explore the potential of regulating the differentiation of ESCs into pancreatic endocrine cells capable of synthesizing the pancreatic hormones including insulin, glucagon, somatostatin and pancreatic polypeptide under proper conditions. Undifferentiated ES cell line was stably transfected with mouse RIP-YFP plasmid construction in serum-free medium using LipofectamineTM 2000 Reagents. We tested pancreatic specific gene expression and characterized these ESC-derived pancreatic endocrine cells. Most of these insulin-secreting cells co-expressed many of the phenotypic markers characteristic of 13 cells such as insulinl, insulin2, Isletl, MafA, insulinoma-associated antigen 1 (IA1) and so on, indicating a similar gene expression pattern to adult islet 13 cells in vivo. Characterization of this population revealed that it consisted predominantly of pancreatic endocrine cells that were able to undergo pancreatic specification under the appropriate conditions. We also demonstrated that zinc supplementation mediated up-regulation of insulin-secreting cells as an effective inducer promoted the development of ESC-derived diabetes therapy. In conclusion, this work not only established an efficient pancreatic differentiation strategy from ESCs to pancreatic endocrine lineage in vitro, but also leaded to the development of new strategies to derive transplantable islet-replacement 13 cells from embryonic stem cells for the future applications of a stem cell based therapy of diabetes. 展开更多
关键词 Embryonic stem cells Pancreatic differentiation Insulin-secreting cells Transcription factors ZINC
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