For the last two decades the world has undergone a population explosion which has been accompanied by a speeding up of urbanization and land use for industrial and agricultural products. These involve a vast increase ...For the last two decades the world has undergone a population explosion which has been accompanied by a speeding up of urbanization and land use for industrial and agricultural products. These involve a vast increase in the discharge of pollutants into very different receiving water bodies, and have had adverse effects on the various components of the environment. For a long time, human has concerned a little of the natural environment. It is obvious that this variation in surface temperature of the earth increases the level of sea due to snowmelt. So climate change is manifested by many phenomena： floods, droughts, fires, tornadoes, cyclones, earthquakes, volcanic eruptions, diseases etc.. The statement today is dramatic. Entire regions were devastated and many rivers and lakes have become polluted around the world. Pollution has it nature which is hard to be absorbed. Where did the different pollutants come from？ What are the risks to the aquatic environment？ To prevent and combat the general decline of these ecosystems, it is important to distinguish and determine the effects of different sources of pollution and any changes that may suffer the physical environment. The climate depends primarily on changes in earth orbit parameters, which affect the solar radiation received by the earth surface. Therefore, human participates strongly to these changes, for example, pollution is the main reason to argue the greenhouse effect, an increase of anthropogenic carbon dioxide （CO2） into the atmosphere, which promotes global warming to the planet. These modifications are already applied in order to ensure durable development in water resources by controlling discharges into streams to preserve water resources for future generations and to ensure reconciliation between man-climate and environment.展开更多
In Mexico, hazardous and non-hazardous urban solid wastes are commonly disposed of in dumps. Inappropriate waste disposal makes solid waste disposal sites potential sources of pollution and health risks. The objective...In Mexico, hazardous and non-hazardous urban solid wastes are commonly disposed of in dumps. Inappropriate waste disposal makes solid waste disposal sites potential sources of pollution and health risks. The objective of the present study was to evaluate the impact of leachate from a dump on surface water systems. Physical, chemical, and bacteriological characteristics and the volume of leachate were measured. The site of study is located in Zinaprcuaro, Michoac^m, Mexico, and was chosen due to the water supplies were in line with the dump, thus conforming a system to test for movement of toxins. Leachate and water from three ponds located at different distances to the dump were sampled during three months of a year to measure physicochemical and bacteriological parameters, according to the standard methods of the American Public Health Association; the concentration of heavy metals was measured by atomic absorption spectrometry. Most characteristics exceed the maximum permissible limits established for the Mexican legislation regarding wastewater discharges and drinking water. Likewise, analysis of variance showed statistically significant differences between concentrations of pollutants in relation to the distance from the dump of sampled sites and with season of sampling, thus confirming the contamination of water by dump leachate.展开更多
Phage contamination is a very serious and unavoidable problem in modem fermentation industry. It is necessary to develop sensitive and rapid phage detection methods for the early detection of phage contamination. In t...Phage contamination is a very serious and unavoidable problem in modem fermentation industry. It is necessary to develop sensitive and rapid phage detection methods for the early detection of phage contamination. In the present work, a real-time, rapid, specific and quantitative phage T4 detection method based on surface plasmon resonance （SPR） technique has been in- troduced. Escherichia coli was immobilized onto the preformed MPA self-assembled monolayer （SAM） through the widely used EDC/NHS cross-linking reaction as the recognition element. The bacteria immobilization was verified efficiently through the electrochemical measurements and fluorescence microscopy observations. The specific adsorption was much stronger than the non-specific adsorption of phage T4 binding to the biosensor surface modified by E. coli, and the latter could be neglected. The detection sensitivity reached 1×10^7 PFU/mL within 10 min. Within the experimental phage concentrations, the linear cor- relation between the SPR response and the phage concentration was good. The results suggest that the SPR technique is a po- tentially powerful tool for the phage or other virus detections, as a label-free, real-time, and rapid method.展开更多
文摘For the last two decades the world has undergone a population explosion which has been accompanied by a speeding up of urbanization and land use for industrial and agricultural products. These involve a vast increase in the discharge of pollutants into very different receiving water bodies, and have had adverse effects on the various components of the environment. For a long time, human has concerned a little of the natural environment. It is obvious that this variation in surface temperature of the earth increases the level of sea due to snowmelt. So climate change is manifested by many phenomena： floods, droughts, fires, tornadoes, cyclones, earthquakes, volcanic eruptions, diseases etc.. The statement today is dramatic. Entire regions were devastated and many rivers and lakes have become polluted around the world. Pollution has it nature which is hard to be absorbed. Where did the different pollutants come from？ What are the risks to the aquatic environment？ To prevent and combat the general decline of these ecosystems, it is important to distinguish and determine the effects of different sources of pollution and any changes that may suffer the physical environment. The climate depends primarily on changes in earth orbit parameters, which affect the solar radiation received by the earth surface. Therefore, human participates strongly to these changes, for example, pollution is the main reason to argue the greenhouse effect, an increase of anthropogenic carbon dioxide （CO2） into the atmosphere, which promotes global warming to the planet. These modifications are already applied in order to ensure durable development in water resources by controlling discharges into streams to preserve water resources for future generations and to ensure reconciliation between man-climate and environment.
文摘In Mexico, hazardous and non-hazardous urban solid wastes are commonly disposed of in dumps. Inappropriate waste disposal makes solid waste disposal sites potential sources of pollution and health risks. The objective of the present study was to evaluate the impact of leachate from a dump on surface water systems. Physical, chemical, and bacteriological characteristics and the volume of leachate were measured. The site of study is located in Zinaprcuaro, Michoac^m, Mexico, and was chosen due to the water supplies were in line with the dump, thus conforming a system to test for movement of toxins. Leachate and water from three ponds located at different distances to the dump were sampled during three months of a year to measure physicochemical and bacteriological parameters, according to the standard methods of the American Public Health Association; the concentration of heavy metals was measured by atomic absorption spectrometry. Most characteristics exceed the maximum permissible limits established for the Mexican legislation regarding wastewater discharges and drinking water. Likewise, analysis of variance showed statistically significant differences between concentrations of pollutants in relation to the distance from the dump of sampled sites and with season of sampling, thus confirming the contamination of water by dump leachate.
基金support from the National Basic Research Program of China (2011CB933600)the National Natural Science Foundation of China (21077081,20921062)+1 种基金the Natural Science Foundation of Hubei Province (2010CDB01302)the Fundamental Research Funds for Central Universities (1103005 and 1101007)
文摘Phage contamination is a very serious and unavoidable problem in modem fermentation industry. It is necessary to develop sensitive and rapid phage detection methods for the early detection of phage contamination. In the present work, a real-time, rapid, specific and quantitative phage T4 detection method based on surface plasmon resonance （SPR） technique has been in- troduced. Escherichia coli was immobilized onto the preformed MPA self-assembled monolayer （SAM） through the widely used EDC/NHS cross-linking reaction as the recognition element. The bacteria immobilization was verified efficiently through the electrochemical measurements and fluorescence microscopy observations. The specific adsorption was much stronger than the non-specific adsorption of phage T4 binding to the biosensor surface modified by E. coli, and the latter could be neglected. The detection sensitivity reached 1×10^7 PFU/mL within 10 min. Within the experimental phage concentrations, the linear cor- relation between the SPR response and the phage concentration was good. The results suggest that the SPR technique is a po- tentially powerful tool for the phage or other virus detections, as a label-free, real-time, and rapid method.
