Chitinases play an important role in regulating plant growth and development, especially defending themselves from fungal pathogens. It is important to do the biological analyses in crops. In this study, the result sh...Chitinases play an important role in regulating plant growth and development, especially defending themselves from fungal pathogens. It is important to do the biological analyses in crops. In this study, the result showed that the chitinases were distributed into the whole genome in rice, and nearly the whole genome in maize expect for Chromosome 9. The clustering results showed that one out of three chitinases from maize and rice belonged to new groups, which were separated from those in the conformed Classes I-VII. The identification of most amino acid sequences was very low among the chitinases from rice and/or maize. It was inferred that the chitinases with different functions were relatively stable during plant evolution. The relationship of chitinases expression between leaf blade and anther was positively significant in maize, but not significant in rice. Additionally, the ratio of chitinases with up- or down-regulated expression in sensitive maize under Fusarium moniliforme inoculation was different from that in sensitive rice under Magnaporthe grisea inoculation. It might be result from different tissues infected by different fungi. The number of chitinases from resistant maize was less than that from sensitive maize, which inferred that the resistant pathways on F. moniliforme should be not only chitin induced Pathogen-associated molecular PTI （patterns-triggered immunity） pathway, but also might include other PTI pathways that improve tolerance to F. moniliforme. The analysis of expression pattern of chitinases from maize and rice under fungi inoculation will be contributed into further research on the defense mechanism of fungi in crops.展开更多
A pot experiment was conducted to investigate the action mechanisms phorus （P） uptake of Capsicum annuum L. in a sterilized fossil Oxisol of arbuscular mycorrhizal （AM） fungi in phos- Three P levels of 0, 10 and 2...A pot experiment was conducted to investigate the action mechanisms phorus （P） uptake of Capsicum annuum L. in a sterilized fossil Oxisol of arbuscular mycorrhizal （AM） fungi in phos- Three P levels of 0, 10 and 200 mg kg-1 soil （P0, P10 and P200, respectively） without and with AM fungal inoculation were applied as Ca（H2PO4）2-H20. Shoot dry matter yields and shoot P uptake increased significantly （P 〉 0.05） by the inoculation of AM fungi at P0 and P10. Root length and P concentration in soil solution increased with the inoculation of AM fungi but the root：shoot ratio decreased or remained constant. Around 50% roots of inoculated plants were infected by AM and the external hyphae amounted to 20 m g^-1 soil at P10 and P200. The hyphae surface area of the infected root cylinder amounted to 11 and 2 cm^2 cm^-2 root at P0 and P10, respectively. The increased P uptake of inoculated plants was mainly because of an up to 5 times higher P influx of the infected root. Model calculations showed that the root alone could not have achieved the measured P influx in both infected and non-infected roots. But the P influx for hyphae calculated by the model was even much higher than the measured one. The P uptake capacity of hyphae introduced in the model was too high. Model calculations further showed that the depletion zone around roots or hyphae was very narrow. In the case of the root only 7% of the soil volume would contribute P to the plant, while in the case of hyphae it would be 100%. The results together with the model calculations showed that the increased P uptake of AM inoculated plants could be explained partly by the increased P concentration in the soil solution and by the increased P absorbing surface area coming from the external hyphae.展开更多
基金Acknowledgments This work was supported by Specialized Research Fund for New Teachers of Doctoral Program in the University of China （No. 20125103120011） and the Natural National Science Foundation of China （No. 31201274）.
文摘Chitinases play an important role in regulating plant growth and development, especially defending themselves from fungal pathogens. It is important to do the biological analyses in crops. In this study, the result showed that the chitinases were distributed into the whole genome in rice, and nearly the whole genome in maize expect for Chromosome 9. The clustering results showed that one out of three chitinases from maize and rice belonged to new groups, which were separated from those in the conformed Classes I-VII. The identification of most amino acid sequences was very low among the chitinases from rice and/or maize. It was inferred that the chitinases with different functions were relatively stable during plant evolution. The relationship of chitinases expression between leaf blade and anther was positively significant in maize, but not significant in rice. Additionally, the ratio of chitinases with up- or down-regulated expression in sensitive maize under Fusarium moniliforme inoculation was different from that in sensitive rice under Magnaporthe grisea inoculation. It might be result from different tissues infected by different fungi. The number of chitinases from resistant maize was less than that from sensitive maize, which inferred that the resistant pathways on F. moniliforme should be not only chitin induced Pathogen-associated molecular PTI （patterns-triggered immunity） pathway, but also might include other PTI pathways that improve tolerance to F. moniliforme. The analysis of expression pattern of chitinases from maize and rice under fungi inoculation will be contributed into further research on the defense mechanism of fungi in crops.
基金Supported by the Higher Education Commission of Pakistan
文摘A pot experiment was conducted to investigate the action mechanisms phorus （P） uptake of Capsicum annuum L. in a sterilized fossil Oxisol of arbuscular mycorrhizal （AM） fungi in phos- Three P levels of 0, 10 and 200 mg kg-1 soil （P0, P10 and P200, respectively） without and with AM fungal inoculation were applied as Ca（H2PO4）2-H20. Shoot dry matter yields and shoot P uptake increased significantly （P 〉 0.05） by the inoculation of AM fungi at P0 and P10. Root length and P concentration in soil solution increased with the inoculation of AM fungi but the root：shoot ratio decreased or remained constant. Around 50% roots of inoculated plants were infected by AM and the external hyphae amounted to 20 m g^-1 soil at P10 and P200. The hyphae surface area of the infected root cylinder amounted to 11 and 2 cm^2 cm^-2 root at P0 and P10, respectively. The increased P uptake of inoculated plants was mainly because of an up to 5 times higher P influx of the infected root. Model calculations showed that the root alone could not have achieved the measured P influx in both infected and non-infected roots. But the P influx for hyphae calculated by the model was even much higher than the measured one. The P uptake capacity of hyphae introduced in the model was too high. Model calculations further showed that the depletion zone around roots or hyphae was very narrow. In the case of the root only 7% of the soil volume would contribute P to the plant, while in the case of hyphae it would be 100%. The results together with the model calculations showed that the increased P uptake of AM inoculated plants could be explained partly by the increased P concentration in the soil solution and by the increased P absorbing surface area coming from the external hyphae.
