目的:探究神经松动术联合头体针巨刺法应用于卒中后偏瘫患者的效果。方法:选取2019年1月至2021年8月多中心收治的126例卒中后偏瘫患者,按照治疗方案不同随机分为联合组(n=42)、头体针组(n=42),神经松动组(n=42),头体针组患者进行头体针...目的:探究神经松动术联合头体针巨刺法应用于卒中后偏瘫患者的效果。方法:选取2019年1月至2021年8月多中心收治的126例卒中后偏瘫患者,按照治疗方案不同随机分为联合组(n=42)、头体针组(n=42),神经松动组(n=42),头体针组患者进行头体针巨刺法,神经松动组进行手法神经松动术,联合组患者在进行头体针巨刺法基础上进行手法神经松动术;3组患者在治疗前、治疗后4周、8周进行美国国立卫生研究院卒中量表(National Institute of Health stroke scale,NIHSS)评估神经功能,Berg平衡量表(Berg Balance Scale,BBS)评估肢体平衡功能,采用肌电图诱发电位仪检测患侧正中神经波幅、阈值,并应用Fugl-Meyer运动功能评定量表(Fugl-MeyerAssessmentScale,FMAS)评估肢体运动功能,barthel指数(Modified barthel index,MBI)评分、计时起立-行走测试(Time Up and Go Test,TUGT)评分评估日常活动能力。结果:与同组治疗前相比,治疗后4周、8周时3组患者的NIHSS评分、水肿分级评分、肩部疼痛VAS评分均降低(各项均P<0.05),BBS评分、上肢运动、下肢运动评分、MBI评分均升高(各项均P<0.05);患侧正中神经F波阈值降低,F波波幅升高,TUGT时间缩短(P<0.05);组间相比,治疗4周、8周后联合组NIHSS评分较头体针组、神经松动组低(P<0.05),BBS评分、肢体运动功能评分较头体针组、神经松动组高(P<0.05);联合组患侧正中神经F波阈值低于头体针组、神经松动组(P<0.05);F波波幅高于头体针组、神经松动组(P<0.05);联合组水肿分级评分、肩部疼痛VAS评分较头体针组、神经松动组低(P<0.05);联合组TUGT时间短于头体针组、神经松动组,MBI评分较头体针组、神经松动组高(P<0.05)。结论:头体针巨刺法与神经松动术联合应用于卒中后偏瘫可有效缓解神经损伤,促进肢体运动功能恢复,提高平衡功能及日常活动能力。展开更多
AIM: To evaluate the level of sperm chromosome aberrations in male patients with hepatitis B, and to directly detect whether there are HBV DNA integrations in sperm chromosomes of hepatitis B patients.METHODS: Sperm c...AIM: To evaluate the level of sperm chromosome aberrations in male patients with hepatitis B, and to directly detect whether there are HBV DNA integrations in sperm chromosomes of hepatitis B patients.METHODS: Sperm chromosomes of 14 tested subjects (5healthy controls, 9 patients with HBV infection, including 1with acute hepatitis B, 2 with chronic active hepatitis B, 4with chronic persistent hepatitis B, 2 chronic HBsAg carriers with no clinical symptoms) were prepared using interspecific in vitro fertilization between zona-free golden hamster ova and human spermatozoa, and the frequencies of aberration spermatozoa were compared between subjects of HBV infection and controls. Fluorescence in situ hybridization (FISH) to sperm chromosome spreads was carried out with biotin-labeled full length HBV DNA probe to detect the specific HBV DNA sequences in the sperm chromosomes.RESULTS: The total frequency of sperm chromosome aberrations in HBV infection group (14.8%, 33/223) was significantly higher than that in the control group (4.3%,5/116). Moreover, the sperm chromosomes in HBV infection patients commonly presented stickiness, clumping, failure to staining, etc, which would affect the analysis of sperm chromosomes. Specific fluorescent signal spots for HBV DNA were seen in sperm chromosomes of one patient with chronic persistent hepatitis. In 9 (9/42) sperm chromosome complements containing fluorescent signal spots, one presented 5 obvious FISH spots, others presented 2 to 4signals. There was significant difference of fluorescence intensity among the signal spots. The distribution of signal sites among chromosomes was random.CONCLUSION: HBV infection can bring about mutagenic effects on sperm chromosomes. Integrations of viral DNA into sperm chromosomes which are multisites and nonspecific, can further increase the instability of sperm chromosomes. This study suggested that HBV infection can create extensively hereditary effects by alteration genetic constituent and/or induction chromosome aberrations, as well as the possibility of vertical transmission of HBV via the germ line to the next generation.展开更多
Objective:To observe the clinical efficacy of body acupuncture combined with FANG’s scalp acupuncture in treating limb dysfunction in the remission stage of stroke and explore the mechanism.Methods:Sixty patients in ...Objective:To observe the clinical efficacy of body acupuncture combined with FANG’s scalp acupuncture in treating limb dysfunction in the remission stage of stroke and explore the mechanism.Methods:Sixty patients in the remission stage of stroke with limb dysfunction were divided into a control group and a treatment group using the random number table method,with 30 cases in each group.The control group was treated with ordinary acupuncture based on the conventional rehabilitation treatment,and the treatment group was additionally offered FANG’s scalp acupuncture.The interventions were conducted once daily with 10 sessions as 1 course.The Fugl-Meyer assessment(FMA)and Barthel index(BI)scores and serum malondialdehyde(MDA)and superoxide dismutase(SOD)levels were compared after 2 treatment courses,and the clinical efficacy was evaluated.Results:After treatment,the FMA and BI scores increased in both groups(P<0.05)and were higher in the treatment group than in the control group(P<0.05).Both groups showed a decreased MDA level and an increased SOD level after the intervention,all showing statistical significance(P<0.05);there were significant differences between the two groups(P<0.05).Conclusion:Based on the conventional rehabilitation treatment,ordinary acupuncture used alone or in combination with FANG’s scalp acupuncture can lower the oxidative stress level and improve limb function in treating limb dysfunction in the remission stage of stroke;body acupuncture plus FANG’s scalp acupuncture can produce better results.展开更多
Objective To observe the effects of eye-acupuncture therapy and bodyacupuncture therapy on the expression of brain-deprived neurotrophic factor （BDNF） in rats with cerebral ischemia reperfusion injury （CIRI）. Meth...Objective To observe the effects of eye-acupuncture therapy and bodyacupuncture therapy on the expression of brain-deprived neurotrophic factor （BDNF） in rats with cerebral ischemia reperfusion injury （CIRI）. Methods According to random number table, 48 SD rats were randomly divided into 6 groups, including normal control group （group A）, sham operation group （group B）, model group （group C）, eye-acupuncture group （group D）, non-acupoint of eye-acupuncture group （group E） and body-acupuncture group （group F）, eight rats in each group. Artery infarction reperfusion model were prepared by using suture-occluded method. Liver region, upper energizer area, lower energizer area and kidney region were selected in the group D. Acupuncture was carried out at the point located at 3 mm from the acupoint areas in the group E. Qūchí （曲池 LI 11）, Zúsānl （足三里 ST 36） and other acupoints were selected in the group F. Zea Longa scoring method was utilized for scoring the neural functions of rats; real-time PCR was carried out to examine the expression level of BDNF mRNA in the brain 72 h after ischemia reperfusion; western blot was carried out to examine the expression level of BDNF protein in the brain 72 h after ischemia reperfusion. Results The symptoms of neurologic impairments in the rats of the group D were alleviated in comparison to those in the group C （P0.01）, and the difference between the group D and the group F was not statistically significant （P0.05）; Compared with the group C, the mRNA and protein expression levels of BDNF in the brain of rats in the group D and the group F both increased （P0.01）, but the difference between the group D and the group F was not statistically significant （P0.05）. Conclusion The functions of eye-acupuncture and body-acupuncture in improving cerebral ischemia reperfusion injury are similar, and the functional mechanisms for the two different therapies may be related to the up-regulation of BDNF expression in brain and thus promote the repairing of brain tissues.展开更多
文摘目的:探究神经松动术联合头体针巨刺法应用于卒中后偏瘫患者的效果。方法:选取2019年1月至2021年8月多中心收治的126例卒中后偏瘫患者,按照治疗方案不同随机分为联合组(n=42)、头体针组(n=42),神经松动组(n=42),头体针组患者进行头体针巨刺法,神经松动组进行手法神经松动术,联合组患者在进行头体针巨刺法基础上进行手法神经松动术;3组患者在治疗前、治疗后4周、8周进行美国国立卫生研究院卒中量表(National Institute of Health stroke scale,NIHSS)评估神经功能,Berg平衡量表(Berg Balance Scale,BBS)评估肢体平衡功能,采用肌电图诱发电位仪检测患侧正中神经波幅、阈值,并应用Fugl-Meyer运动功能评定量表(Fugl-MeyerAssessmentScale,FMAS)评估肢体运动功能,barthel指数(Modified barthel index,MBI)评分、计时起立-行走测试(Time Up and Go Test,TUGT)评分评估日常活动能力。结果:与同组治疗前相比,治疗后4周、8周时3组患者的NIHSS评分、水肿分级评分、肩部疼痛VAS评分均降低(各项均P<0.05),BBS评分、上肢运动、下肢运动评分、MBI评分均升高(各项均P<0.05);患侧正中神经F波阈值降低,F波波幅升高,TUGT时间缩短(P<0.05);组间相比,治疗4周、8周后联合组NIHSS评分较头体针组、神经松动组低(P<0.05),BBS评分、肢体运动功能评分较头体针组、神经松动组高(P<0.05);联合组患侧正中神经F波阈值低于头体针组、神经松动组(P<0.05);F波波幅高于头体针组、神经松动组(P<0.05);联合组水肿分级评分、肩部疼痛VAS评分较头体针组、神经松动组低(P<0.05);联合组TUGT时间短于头体针组、神经松动组,MBI评分较头体针组、神经松动组高(P<0.05)。结论:头体针巨刺法与神经松动术联合应用于卒中后偏瘫可有效缓解神经损伤,促进肢体运动功能恢复,提高平衡功能及日常活动能力。
基金the Natural Science Foundation of Guangdong Province,No.940567the National Natural Science Foundation of China,No.39970374
文摘AIM: To evaluate the level of sperm chromosome aberrations in male patients with hepatitis B, and to directly detect whether there are HBV DNA integrations in sperm chromosomes of hepatitis B patients.METHODS: Sperm chromosomes of 14 tested subjects (5healthy controls, 9 patients with HBV infection, including 1with acute hepatitis B, 2 with chronic active hepatitis B, 4with chronic persistent hepatitis B, 2 chronic HBsAg carriers with no clinical symptoms) were prepared using interspecific in vitro fertilization between zona-free golden hamster ova and human spermatozoa, and the frequencies of aberration spermatozoa were compared between subjects of HBV infection and controls. Fluorescence in situ hybridization (FISH) to sperm chromosome spreads was carried out with biotin-labeled full length HBV DNA probe to detect the specific HBV DNA sequences in the sperm chromosomes.RESULTS: The total frequency of sperm chromosome aberrations in HBV infection group (14.8%, 33/223) was significantly higher than that in the control group (4.3%,5/116). Moreover, the sperm chromosomes in HBV infection patients commonly presented stickiness, clumping, failure to staining, etc, which would affect the analysis of sperm chromosomes. Specific fluorescent signal spots for HBV DNA were seen in sperm chromosomes of one patient with chronic persistent hepatitis. In 9 (9/42) sperm chromosome complements containing fluorescent signal spots, one presented 5 obvious FISH spots, others presented 2 to 4signals. There was significant difference of fluorescence intensity among the signal spots. The distribution of signal sites among chromosomes was random.CONCLUSION: HBV infection can bring about mutagenic effects on sperm chromosomes. Integrations of viral DNA into sperm chromosomes which are multisites and nonspecific, can further increase the instability of sperm chromosomes. This study suggested that HBV infection can create extensively hereditary effects by alteration genetic constituent and/or induction chromosome aberrations, as well as the possibility of vertical transmission of HBV via the germ line to the next generation.
文摘Objective:To observe the clinical efficacy of body acupuncture combined with FANG’s scalp acupuncture in treating limb dysfunction in the remission stage of stroke and explore the mechanism.Methods:Sixty patients in the remission stage of stroke with limb dysfunction were divided into a control group and a treatment group using the random number table method,with 30 cases in each group.The control group was treated with ordinary acupuncture based on the conventional rehabilitation treatment,and the treatment group was additionally offered FANG’s scalp acupuncture.The interventions were conducted once daily with 10 sessions as 1 course.The Fugl-Meyer assessment(FMA)and Barthel index(BI)scores and serum malondialdehyde(MDA)and superoxide dismutase(SOD)levels were compared after 2 treatment courses,and the clinical efficacy was evaluated.Results:After treatment,the FMA and BI scores increased in both groups(P<0.05)and were higher in the treatment group than in the control group(P<0.05).Both groups showed a decreased MDA level and an increased SOD level after the intervention,all showing statistical significance(P<0.05);there were significant differences between the two groups(P<0.05).Conclusion:Based on the conventional rehabilitation treatment,ordinary acupuncture used alone or in combination with FANG’s scalp acupuncture can lower the oxidative stress level and improve limb function in treating limb dysfunction in the remission stage of stroke;body acupuncture plus FANG’s scalp acupuncture can produce better results.
基金Supported by National Key Fundamental:Research and Development Project 2007 CB 512702A project of starting foundation for doctors in Liaoning Province:20131073
文摘Objective To observe the effects of eye-acupuncture therapy and bodyacupuncture therapy on the expression of brain-deprived neurotrophic factor （BDNF） in rats with cerebral ischemia reperfusion injury （CIRI）. Methods According to random number table, 48 SD rats were randomly divided into 6 groups, including normal control group （group A）, sham operation group （group B）, model group （group C）, eye-acupuncture group （group D）, non-acupoint of eye-acupuncture group （group E） and body-acupuncture group （group F）, eight rats in each group. Artery infarction reperfusion model were prepared by using suture-occluded method. Liver region, upper energizer area, lower energizer area and kidney region were selected in the group D. Acupuncture was carried out at the point located at 3 mm from the acupoint areas in the group E. Qūchí （曲池 LI 11）, Zúsānl （足三里 ST 36） and other acupoints were selected in the group F. Zea Longa scoring method was utilized for scoring the neural functions of rats; real-time PCR was carried out to examine the expression level of BDNF mRNA in the brain 72 h after ischemia reperfusion; western blot was carried out to examine the expression level of BDNF protein in the brain 72 h after ischemia reperfusion. Results The symptoms of neurologic impairments in the rats of the group D were alleviated in comparison to those in the group C （P0.01）, and the difference between the group D and the group F was not statistically significant （P0.05）; Compared with the group C, the mRNA and protein expression levels of BDNF in the brain of rats in the group D and the group F both increased （P0.01）, but the difference between the group D and the group F was not statistically significant （P0.05）. Conclusion The functions of eye-acupuncture and body-acupuncture in improving cerebral ischemia reperfusion injury are similar, and the functional mechanisms for the two different therapies may be related to the up-regulation of BDNF expression in brain and thus promote the repairing of brain tissues.
