Objective] The aim of this study was to simultaneously isolate and identify the main pathogenic fungi of the root rot, black spot and round spot from the Panax notoginseng plants cultivated in Wenshan Eparchy of Yunna...Objective] The aim of this study was to simultaneously isolate and identify the main pathogenic fungi of the root rot, black spot and round spot from the Panax notoginseng plants cultivated in Wenshan Eparchy of Yunnan Province of China. [Method] The pathogenic fungi were isolated and purified by using potato dextrose agar (PDA) medium. The morphological identification was accomplished first according to the colony forms of the fungi when cultivated in vitro, then accord-ing to the symptom characteristics and colony forms of the re-isolated fungi in the reverse inoculation experiments. The molecular identification was performed accord-ing to the amplification and alignment of the internal transcribed space (ITS) se-quences of the fungi. The increases of the diameters and thickness of the colonies of the fungi cultivated in vitro were employed to indicate the growth rates of the fungi. [Results] The consistency of the colony forms and symptom characteristics and the 96%-99% similarities revealed in the ITS sequence alignments al proved that the main pathogenic fungi of the root rot, black spot and round spot of the P. notoginseng plants raised in Wenshan were Cylindrocarpon didymium, Alternaria panax and Mycocentrospora acerina, respectively. When cultivated in vitro in the same temperature, humidity and il umination, the increases of the colony diameters and thickness of C. didymium were the highest, fol owed by those of A. panax, then those of M. acerina. During different cultivation periods, the differences of the colony diameters and thickness of the three fungi al reached extremely significant level. However, at the same cultivation time, the differences of the diameters and thickness among the three fungi only reached significant level. [Conclusion] The main pathogenic fungi which result in the root rot, black spot and round spot of the P. notoginseng in Wenshan are C. didymium, A. panax and M. acerina, respec-tively. When these three diseases break out at the same time, the root rot wil spread fastest, fol owed orderly by the black spot and the round spot.展开更多
To understand metabolic adaptations,the basal metabolic rate(BMR) of Mrs Hume's Pheasant(Syrmaticus humiae) and Elliot's Pheasant(Syrmaticus ellioti) were investigated.Metabolic rate(MR),body temperature(Tb...To understand metabolic adaptations,the basal metabolic rate(BMR) of Mrs Hume's Pheasant(Syrmaticus humiae) and Elliot's Pheasant(Syrmaticus ellioti) were investigated.Metabolic rate(MR),body temperature(Tb) and thermal conductance(C) were determined in both species at a temperatrue range of 5-35 ℃,respectively.Oxygen consumption was measured with a closed circuit respirometer.The thermal neutral zones(TNZ) were 24.5-31.6 ℃,and 23.0-29.2 ℃,respectively.With a temperature range of 5-35 ℃,Mrs Hume's Pheasant and Elliot's Pheasant could maintained stable Tb at a mean of(40.47±0.64) and(40.36±0.10) ℃,respectively.Mean BMRs within TNZs were(1.36±0.84) mLO2/(g.h) for Mrs Hume's Pheasant and(2.03±0.12) mLO2/(g.h) for Elliot's Pheasant,which were 77% and 86% of the expected value based on their body mass,respectively.Thermal conductance of Mrs Hume's Pheasant and Elliot's Pheasant were(0.12±0.01) and(0.17±0.01) mLO2/(g.h.℃),below the lower critical temperature,respectively,which were 119% and 124% of the expected value based on their body mass,respectively.The ecophysiological characteristics of these species were low metabolic rate,high body temperature,and high thermal conductance,which allow both species to better adapt to the warmer climate environment in south China.展开更多
Room-temperature mechanical properties of Cu50Zr40Ti10-xNix(0≤x≤4,mole fraction,%) bulk metallic glasses (BMG) with aspect ratios in the range of 1:1-2.5:1 and loading rates in the range of1×10^-5-1×...Room-temperature mechanical properties of Cu50Zr40Ti10-xNix(0≤x≤4,mole fraction,%) bulk metallic glasses (BMG) with aspect ratios in the range of 1:1-2.5:1 and loading rates in the range of1×10^-5-1×10^-2s^-1were systematically investigated by room-temperatureuniaxialcompression test.In the condition of an aspect ratio of 1:1, the superplasticity can be clearly observed for Cu50Zr40Ti10BMG when the loading rate is1×10^-4s^-1, while for Cu50Zr40Ti10-xNix(x=1-3, mole fraction, %) BMGs when the loading rate is1×10^-2s^-1. The plastic strain (εp), yielding strength (σy) and fracture strength (σf) of the studied Cu-based BMGs significantly depend on the aspect ratio and the loading rate. In addition, theσyof the studied Cu-based BMGs with an aspect ratio of 1:1 is close to the σfof those with the other aspect ratios when the loading rate is1×10^-2s^-1. The mechanism for the mechanical response to the loading rate and the aspect ratiowas also discussed.展开更多
Based on the population balance equation in a batch crystallizer characteristic of seeded precipitation, a model to calculate the rate of apparent crystal growth of aluminum hydroxide from the size distribution was de...Based on the population balance equation in a batch crystallizer characteristic of seeded precipitation, a model to calculate the rate of apparent crystal growth of aluminum hydroxide from the size distribution was deve- loped. The simulation results indicate that the rate of apparent crystal growth during seeded precipitation exhibits a manifest dependence on the crystal size. In general, there is an obvious increase in the apparent crystal growth rate with the augment in crystal size. The apparent activation energy increases with the increase of characteristic crystal size, which indicates that the growth of small crystals is controlled by surface chemical reaction; it is gradually controlled by both the surface reaction and diffusion with the augment in crystal size.展开更多
The architecture of the panicle, including grain size and panicle morphology, directly determines grain yield. Panicle erectness, which is selected for achieving ideal plant arehitecture in the northern part of China,...The architecture of the panicle, including grain size and panicle morphology, directly determines grain yield. Panicle erectness, which is selected for achieving ideal plant arehitecture in the northern part of China, has drawn increasing attention of rice breeders. Here, dense and erect panicle 2 (dep2) mutant, which shows a dense and erect panicle phenotype, was identified. DEP2 encodes a plant-specific protein without any known functional domain. Expression profiling of DEP2 revealed that it is highly expressed in young tissues, with most abundance in young panicles. Morphological and expression analysis indicated that mutation in DEP2 mainly affects the rapid elongation of rachis and primary and secondary branches, but does not impair the initiation or formation of panicle primordia. Further analysis suggests that decrease of panicle length in dep2 is caused by a defect in cell proliferation during the exponential elongation of panicle. Despite a more compact plant type in the dep2 mutant, no significant alteration in grain production was found between wild type and dep2 mutant. Therefore, the study of DEP2 not only strengthens our understanding of the molecular genetic basis of panicle architecture but also has important implications for rice breeding.展开更多
In laboratory conditions,effects of rearing temperature and stocking density were examined on hatching of fertilized egg and growth of auricularia larvae of Apostichopus japonicus respectively.Data series like larval ...In laboratory conditions,effects of rearing temperature and stocking density were examined on hatching of fertilized egg and growth of auricularia larvae of Apostichopus japonicus respectively.Data series like larval length and density,metamorphic time,and survival rate of the larvae were recorded.Statistics showed that for A.japonicus,survival rate(from fertilized egg to late auricularia) decreased significantly with the increasing rearing temperature(P<0.05).At different temperatures SGR was statistically significant as well(P<0.05) from day 1,and maximal SGR was found on day 9 at 24℃(159.26±3.28).This study clearly indicated that at low temperature(<24℃),metamorphic rate was remarkably higher than at higher temperature(>26℃).Hatching rate was significantly different between 0.2-5 ind./ml groups and 20-50 ind./ml groups.Rearing larvae at the higher density had the smaller maximal-length,whereas needed longer time to complete metamorphosis.This study suggested that 21℃ and 0.4 ind./ml can be used as the most suitable rearing temperature and stocking density for large-scale artificial breeding of A.japonicus's larvae.展开更多
This article is concerned with the numerical investigation of one-dimensional population balance models for batch crystallization process with fines dissolution.In batch crystallization,dissolution of smaller unwanted...This article is concerned with the numerical investigation of one-dimensional population balance models for batch crystallization process with fines dissolution.In batch crystallization,dissolution of smaller unwanted nuclei below some critical size is of vital importance as it improves the quality of product.The crystal growth rates for both size-independent and size-dependent cases are considered.A delay in recycle pipe is also included in the model.The space–time conservation element and solution element method,originally derived for non-reacting flows,is used to solve the model.This scheme has already been applied to a range of PDEs,mainly in the area of fluid mechanics.The numerical results are compared with those obtained from the Koren scheme,showing that the proposed scheme is more efficient.展开更多
Three color variants of the sea cucumber,Apostichopus japonicus are recognized,the red one is highly valued in the market. When the red variant is cultured in ponds in China,its body color changes from red to celadon ...Three color variants of the sea cucumber,Apostichopus japonicus are recognized,the red one is highly valued in the market. When the red variant is cultured in ponds in China,its body color changes from red to celadon in 3–6 months. The effects of water depth and substrate color on the growth and body color of this animal were investigated. Juveniles of red A. japonicus were cultured in cages suspended at a range of water depths(20,50,100,150 and 200 cm). The specific growth rate of red sea cucumbers was significantly higher in animals cultured at deeper water layers compared with those grown at shallowers. Body weights were greatest for sea cucumbers cultured at a depth of 150 cm and their survival rates were highest at a depth of 200 cm. A scale to evaluate the color of red sea cucumbers(R value) was developed using a Pantone standard color card. All stocked animals in the 9-month trial retained a red color,however the red body color was much more intense in sea cucumbers cultured at shallower depths,while animals suspended in deeper layers became pale. In a separate trial,A. japonicus were cultured in suspended cages with seven different colored substrates. Substrate color had a significant effect on the growth and body-color of red A. japonicus. The yield were greatest for A. japonicus cultured on a yellow substrate,followed by green > white > orange > red > black and blue. All sea cucumbers in the 7-month trial retained a red color,although the red was most intense(highest R value) in animals cultured on a blue substrate and pale(lowest R value) for animals cultured on a green substrate.展开更多
Effects of the amplitude(± 2, ± 4, ± 6, and ± 8) and frequency(2, 4, and 8 d) of salinity fluctuation on the body composition and energy budget of juvenile tongue sole(Cynoglossus semilaevis) were ...Effects of the amplitude(± 2, ± 4, ± 6, and ± 8) and frequency(2, 4, and 8 d) of salinity fluctuation on the body composition and energy budget of juvenile tongue sole(Cynoglossus semilaevis) were investigated in a 64-d experiment. Results showed that the amplitude and frequency of salinity fluctuation had significant interaction and both substantially affected the final weight and specific growth rate of juvenile tongue sole. The tongue sole exhibited better growth in treatments with moderate amplitude and frequency of salinity fluctuation(amplitude ± 4–6; frequency 4–8 d) than in other treatments and the control. In terms of energy budget, salinity fluctuation strongly affected the proportions of energy components, including those deposited for growth and lost in respiration, feces, and excretion. Moderately amplitude and frequency of salinity fluctuationg that favored the growth of tongue sole partitioned more energy for growth and less energy for metabolism than the constant and other amplitude and frequency of salinity fluctuation. Average energy budget for tongue sole at moderately fluctuating salinity was determined to be 100C(food) = 30.92G(growth) + 10.30F(feces) + 6.77U(excretion) + 52.01R(respiration). Energetic advantage at moderately fluctuating salinity, including increased energy intake, high assimilation efficiency, reduced metabolism expenditure, and more energy partitioned into growth, might account for the enhancement of tongue sole growth. Commercial farmers are recommended to rear juvenile tongue sole with moderate salinity fluctuations for better growth performance of this species.展开更多
Mg-4 Li-3(Al-Si),Mg-8 Li-3(Al-Si)and Mg-12 Li-3(Al-Si)alloys based on theα-Mg,α-Mg+β-Li,β-Li phases,respectively,were produced to investigate the effect of alloying with Al-Si eutectic on the microstructure and me...Mg-4 Li-3(Al-Si),Mg-8 Li-3(Al-Si)and Mg-12 Li-3(Al-Si)alloys based on theα-Mg,α-Mg+β-Li,β-Li phases,respectively,were produced to investigate the effect of alloying with Al-Si eutectic on the microstructure and mechanical properties of the three alloys.Alloying with the Al-Si eutectic of Mg-xLi(x=4,8 and 12 wt.%)alloys caused the formation of different types of Al-Li precipitates:Al3Li,AlLi and Li3Al2,respectively.Also,considerable quantities of Mg2Si phase particles were found in the three alloys.The results of tensile tests showed that the Mg-4Li-3(Al-Si)alloy exhibited the highest ultimate tensile strength(UTS)of 249 MPa but the lowest elongation of 6.3%.The Mg-12Li-3(Al-Si)alloy had the highest elongation of 26%but the lowest UTS of 173 MPa.The different mechanical properties were attributed to the different crystal structures of the three alloys and the precipitate with different type,morphology and distribution.展开更多
We evaluated the effects of ultraviolet-B (UV-B) radiation and different light conditions on the repair of UV-B-induced damage in carpospores of Chondrus ocellatus Holm (Rhodophyta) in laboratory experiments. Carp...We evaluated the effects of ultraviolet-B (UV-B) radiation and different light conditions on the repair of UV-B-induced damage in carpospores of Chondrus ocellatus Holm (Rhodophyta) in laboratory experiments. Carpospores were treated daily with different doses of UV-B radiation for 48 days, when vertical branches had formed in all treatments; after each daily treatment, the carpospores were subjected to photosynthetically active radiation (PAR), darkness, red light, or blue light during a 2-h repair stage. Carpospore diameters were measured every 4 days. We measured the growth and cellular contents of cyclobutane pyrimidine dimers (CPDs), chlorophyll a, phycoerythrin, and UV-B-absorbing mycosporine-like amino acids (MAAs) in carpospores on Day 48. Low doses of UV-B radiation (36 and 72 J/m2) accelerated the growth of C. ocellatus. However, as the amount of UV-B radiation increased, the growth rate decreased and morphological changes occurred. UV-B radiation significant damaged DNA and photosynthetic pigments and induced three kind of MAAs, palythine, asterina-330, and shinorine. PAR conditions were best for repairing UV-B-induced damage. Darkness promoted the activity of the DNA dark- repair mechanism. Red light enhanced phycoerythrin synthesis but inhibited light repair of DNA. Although blue light, increased the activity of DNA photolyase, greatly improving remediation efficiency, the growth and development of C. ocellatus earpospores were slower than in other light treatments.展开更多
The effects of the artificial-diversion of the Yellow River Mouth for extensive petroleum exploration beginning in August 1996 on the morphology of the mud flat coast are discussed. Changes in the morphology of the co...The effects of the artificial-diversion of the Yellow River Mouth for extensive petroleum exploration beginning in August 1996 on the morphology of the mud flat coast are discussed. Changes in the morphology of the coastal zone were monitored during the period from 1996 to 2000. A Golden Software system was used to calculate the differential surfaces between the surveys in order to investigate the local patterns of accretion and erosion and to determine the volume changes between consecutive surveys. Volumetric studies showed that the net accretion in the study period was about 1.7×10 8 m 3. Between July 1996 and October 1996, a time interval characterized by intense runoff, an accumulation of about 4×10 8 m 3 occurred. In the period from October 1996 to October 1998, a period of diminution in runoff and sediment supply in the upstream, an erosion of 8.9×10 7 m 3 occurred, indicating sediment starvation of the area. Between October 1998 and October 2000, due to an inadequate supply of runoff and sediment, about 1.4×10 8 m 3 of sediments was eroded and transported offshore.展开更多
During transcription initiation,RNA polymerase binds tightly to the promoter DNA defining the start of transcription,transcribes comparatively slowly,and frequently releases short transcripts(3-8 nucleotides)in a proc...During transcription initiation,RNA polymerase binds tightly to the promoter DNA defining the start of transcription,transcribes comparatively slowly,and frequently releases short transcripts(3-8 nucleotides)in a process called abortive cycling.Transitioning to elongation,the second phase of transcription,the polymerase dissociates from the promoter while RNA synthesis continues.Elongation is characterized by higher rates of transcription and tight binding to the RNA transcript.The RNA polymerase from enterophage T7 (T7 RNAP) has been used as a model to understand the mechanism of transcription in general,and the transition from initiation to elongation specifically.This single-subunit enzyme undergoes dramatic conformational changes during this transition to support the changing requirements of nucleic acid interactions while continuously maintaining polymerase function.Crystal structures,available of multiple stages of the initiation complex and of the elongation complex,combined with biochemical and biophysical data,offer molecular detail of the transition.Some of the crystal structures contain a variant of T7 RNAP where proline 266 is substituted by leucine.This variant shows less abortive products and altered timing of transition,and is a valuable tool to study these processes.The structural transitions from early to late initiation are well understood and are consistent with solution data.The timing of events and the structural intermediates in the transition from late initiation to elongation are less well understood,but the available data allows one to formulate testable models of the transition to guide further research.展开更多
The cohesive solids in liquid flows are featured by the dynamic growth and breakage of agglomerates, and the difficulties in the development, design and optimization of these systems are related to this significant fe...The cohesive solids in liquid flows are featured by the dynamic growth and breakage of agglomerates, and the difficulties in the development, design and optimization of these systems are related to this significant feature.In this paper, discrete particle method is used to simulate a solid–liquid flow system including millions of cohesive particles, the growth rate and breakage rate of agglomerates are then systematically investigated. It was found that the most probable size of the agglomerates is determined by the balance of growth and breakage of the agglomerates the cross point of the lines of growth rate and breakage rate as a function of the particle numbers in an agglomerate, marks the most stable agglomerate size. The finding here provides a feasible way to quantify the dynamic behaviors of growth and breakage of agglomerates, and therefore offers the possibility of quantifying the effects of agglomerates on the hydrodynamics of fluid flows with cohesive particles.展开更多
The study on soil thermal conductivity (STC) was an important side of research on ground source heat pump technique,geological disposal of high-level radioactive wastes,heat distribution of buried cable. Especially ow...The study on soil thermal conductivity (STC) was an important side of research on ground source heat pump technique,geological disposal of high-level radioactive wastes,heat distribution of buried cable. Especially owing to technical requirement for shallow terrestrial heat recently, it directly influenced the design and solution in engineering problems. The authors measured the STC in the studied area with QTM-D2 and discussed the effect of samples in size, the measurement error between the samples in lab and in site. The results indicate measuring STC by heat pole method with less influence upon the samples in size, and measuring results on the different geometry size approach very much. The STC is fit for the empirical relation between the temperature and TC under the condition of normal temperature. It is significance for understanding STC in northern China and simulation of temperature field.展开更多
Objective: The purpose of the study was to investigate the effects of parathyroid hormone and parathyroid hormone receptor monoclonal antibody on in vitro growth and proliferation of human medullary thyroid carcinoma...Objective: The purpose of the study was to investigate the effects of parathyroid hormone and parathyroid hormone receptor monoclonal antibody on in vitro growth and proliferation of human medullary thyroid carcinoma cell lines. Methods: The medullary thyroid carcinoma cell line was cultured in vitro, with parathyroid hormone and parathyroid hormone receptor monoclonal antibody treatment intervention, the growth of the cells was observed under an inverted contrast micro scope, the MTT assay was used to detect the cell growth inhibition rate. Results: Under the inverted contrast microscope, the cells changed significantly, the parathyroid hormone and parathyroid hormone receptor monoclonal antibodies can effectively inhibit the proliferation of medullary thyroid cancer cells in a time and dose dependent. When parathyroid hormone concentra tion reached a concentration of 2.0 IJmol/L, the parathyroid hormone receptor monoclonal antibody reached a concentration of 1.0 μmol/L, the cell growth was most significantly inhibited (P 〈 0.05). Conclusion: Parathyroid hormone and parathyroid hormone receptor monoclonal antibody were able to inhibit the proliferation of medullary thyroid carcinoma cells and signifi cantly reduce the proliferation index.展开更多
Objective To investigate fingernail growth rate (FNGR) role in the physiological or pathological status of the fingernails in normal Chinese population. Methods The FNGR was measured with vernier caliper. The data of ...Objective To investigate fingernail growth rate (FNGR) role in the physiological or pathological status of the fingernails in normal Chinese population. Methods The FNGR was measured with vernier caliper. The data of 1 595 fingernails from 208 normal Chinese subjects (including 96 men and 112 women; age ranging from 14 to 78 years) were analyzed. Results The average FNGR was (0.104±0.027) mm per day. Higher growth rates were observed in males than in females, in the young individuals than in the old individuals, in summer than in winter, and in the right hand than in the left hand, respectively. The FNGR differed among fingernails and decreased in order of precedence: middle fingernails, index fingernails or ring fingernails, thumb and little fingernails. Conclusion FNGR was significantly associated with age, gender and temperature. Different fingernail grew at an individual speed.展开更多
in this paper,we prove that a complete n-dimensional Riemannian manifold with nonnegative kth-Ricci curvature, large volume growth has finite topological type provided that lim r→∞{(vol[B(p.r]/ωnrn-αM)rk(n-1...in this paper,we prove that a complete n-dimensional Riemannian manifold with nonnegative kth-Ricci curvature, large volume growth has finite topological type provided that lim r→∞{(vol[B(p.r]/ωnrn-αM)rk(n-1)/k+1(1-α/2)}≤for some COllstant ε〉0 We also prove that a conlplete Riemannian manifold with nonnegative kth-Ricci curvature and undler some pinching conditions is diffeomorphic to R^n.展开更多
基金Supported by the National Natural Science Foundation of China(31060045,31260091,31460065)~~
文摘Objective] The aim of this study was to simultaneously isolate and identify the main pathogenic fungi of the root rot, black spot and round spot from the Panax notoginseng plants cultivated in Wenshan Eparchy of Yunnan Province of China. [Method] The pathogenic fungi were isolated and purified by using potato dextrose agar (PDA) medium. The morphological identification was accomplished first according to the colony forms of the fungi when cultivated in vitro, then accord-ing to the symptom characteristics and colony forms of the re-isolated fungi in the reverse inoculation experiments. The molecular identification was performed accord-ing to the amplification and alignment of the internal transcribed space (ITS) se-quences of the fungi. The increases of the diameters and thickness of the colonies of the fungi cultivated in vitro were employed to indicate the growth rates of the fungi. [Results] The consistency of the colony forms and symptom characteristics and the 96%-99% similarities revealed in the ITS sequence alignments al proved that the main pathogenic fungi of the root rot, black spot and round spot of the P. notoginseng plants raised in Wenshan were Cylindrocarpon didymium, Alternaria panax and Mycocentrospora acerina, respectively. When cultivated in vitro in the same temperature, humidity and il umination, the increases of the colony diameters and thickness of C. didymium were the highest, fol owed by those of A. panax, then those of M. acerina. During different cultivation periods, the differences of the colony diameters and thickness of the three fungi al reached extremely significant level. However, at the same cultivation time, the differences of the diameters and thickness among the three fungi only reached significant level. [Conclusion] The main pathogenic fungi which result in the root rot, black spot and round spot of the P. notoginseng in Wenshan are C. didymium, A. panax and M. acerina, respec-tively. When these three diseases break out at the same time, the root rot wil spread fastest, fol owed orderly by the black spot and the round spot.
基金the National Natural Science Foundation of China (30760039)the Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University)Ministry of Education, China, and the projects of Science and Technology Bureau of Yongzhou, Hunan (201019)
文摘To understand metabolic adaptations,the basal metabolic rate(BMR) of Mrs Hume's Pheasant(Syrmaticus humiae) and Elliot's Pheasant(Syrmaticus ellioti) were investigated.Metabolic rate(MR),body temperature(Tb) and thermal conductance(C) were determined in both species at a temperatrue range of 5-35 ℃,respectively.Oxygen consumption was measured with a closed circuit respirometer.The thermal neutral zones(TNZ) were 24.5-31.6 ℃,and 23.0-29.2 ℃,respectively.With a temperature range of 5-35 ℃,Mrs Hume's Pheasant and Elliot's Pheasant could maintained stable Tb at a mean of(40.47±0.64) and(40.36±0.10) ℃,respectively.Mean BMRs within TNZs were(1.36±0.84) mLO2/(g.h) for Mrs Hume's Pheasant and(2.03±0.12) mLO2/(g.h) for Elliot's Pheasant,which were 77% and 86% of the expected value based on their body mass,respectively.Thermal conductance of Mrs Hume's Pheasant and Elliot's Pheasant were(0.12±0.01) and(0.17±0.01) mLO2/(g.h.℃),below the lower critical temperature,respectively,which were 119% and 124% of the expected value based on their body mass,respectively.The ecophysiological characteristics of these species were low metabolic rate,high body temperature,and high thermal conductance,which allow both species to better adapt to the warmer climate environment in south China.
基金Projects(50874045,51301194)supported by the National Natural Science Foundation of ChinaProject(2144057)supported by the Beijing Natural Science Foundation,China
文摘Room-temperature mechanical properties of Cu50Zr40Ti10-xNix(0≤x≤4,mole fraction,%) bulk metallic glasses (BMG) with aspect ratios in the range of 1:1-2.5:1 and loading rates in the range of1×10^-5-1×10^-2s^-1were systematically investigated by room-temperatureuniaxialcompression test.In the condition of an aspect ratio of 1:1, the superplasticity can be clearly observed for Cu50Zr40Ti10BMG when the loading rate is1×10^-4s^-1, while for Cu50Zr40Ti10-xNix(x=1-3, mole fraction, %) BMGs when the loading rate is1×10^-2s^-1. The plastic strain (εp), yielding strength (σy) and fracture strength (σf) of the studied Cu-based BMGs significantly depend on the aspect ratio and the loading rate. In addition, theσyof the studied Cu-based BMGs with an aspect ratio of 1:1 is close to the σfof those with the other aspect ratios when the loading rate is1×10^-2s^-1. The mechanism for the mechanical response to the loading rate and the aspect ratiowas also discussed.
文摘Based on the population balance equation in a batch crystallizer characteristic of seeded precipitation, a model to calculate the rate of apparent crystal growth of aluminum hydroxide from the size distribution was deve- loped. The simulation results indicate that the rate of apparent crystal growth during seeded precipitation exhibits a manifest dependence on the crystal size. In general, there is an obvious increase in the apparent crystal growth rate with the augment in crystal size. The apparent activation energy increases with the increase of characteristic crystal size, which indicates that the growth of small crystals is controlled by surface chemical reaction; it is gradually controlled by both the surface reaction and diffusion with the augment in crystal size.
基金Supplementary information is linked to the online version of the paper on the Cell Research website. Acknowledgments We thank Professor Gary Loake (University of Edinburg, UK) for critical reading of this manuscript. This work was supported by grants from Ministry of Agriculture of China (2008ZX08001), Ministry of Science and Technology of China (2009CB 118506, 2006AA10A101), and National Natural Science Foundation of China (30671128, 30621001).
文摘The architecture of the panicle, including grain size and panicle morphology, directly determines grain yield. Panicle erectness, which is selected for achieving ideal plant arehitecture in the northern part of China, has drawn increasing attention of rice breeders. Here, dense and erect panicle 2 (dep2) mutant, which shows a dense and erect panicle phenotype, was identified. DEP2 encodes a plant-specific protein without any known functional domain. Expression profiling of DEP2 revealed that it is highly expressed in young tissues, with most abundance in young panicles. Morphological and expression analysis indicated that mutation in DEP2 mainly affects the rapid elongation of rachis and primary and secondary branches, but does not impair the initiation or formation of panicle primordia. Further analysis suggests that decrease of panicle length in dep2 is caused by a defect in cell proliferation during the exponential elongation of panicle. Despite a more compact plant type in the dep2 mutant, no significant alteration in grain production was found between wild type and dep2 mutant. Therefore, the study of DEP2 not only strengthens our understanding of the molecular genetic basis of panicle architecture but also has important implications for rice breeding.
基金Supported by the National High Technology Research and Development Program of China(863 Program)(No.2006AA10A411)Shandong Agriculture Seed Stock Projects
文摘In laboratory conditions,effects of rearing temperature and stocking density were examined on hatching of fertilized egg and growth of auricularia larvae of Apostichopus japonicus respectively.Data series like larval length and density,metamorphic time,and survival rate of the larvae were recorded.Statistics showed that for A.japonicus,survival rate(from fertilized egg to late auricularia) decreased significantly with the increasing rearing temperature(P<0.05).At different temperatures SGR was statistically significant as well(P<0.05) from day 1,and maximal SGR was found on day 9 at 24℃(159.26±3.28).This study clearly indicated that at low temperature(<24℃),metamorphic rate was remarkably higher than at higher temperature(>26℃).Hatching rate was significantly different between 0.2-5 ind./ml groups and 20-50 ind./ml groups.Rearing larvae at the higher density had the smaller maximal-length,whereas needed longer time to complete metamorphosis.This study suggested that 21℃ and 0.4 ind./ml can be used as the most suitable rearing temperature and stocking density for large-scale artificial breeding of A.japonicus's larvae.
文摘This article is concerned with the numerical investigation of one-dimensional population balance models for batch crystallization process with fines dissolution.In batch crystallization,dissolution of smaller unwanted nuclei below some critical size is of vital importance as it improves the quality of product.The crystal growth rates for both size-independent and size-dependent cases are considered.A delay in recycle pipe is also included in the model.The space–time conservation element and solution element method,originally derived for non-reacting flows,is used to solve the model.This scheme has already been applied to a range of PDEs,mainly in the area of fluid mechanics.The numerical results are compared with those obtained from the Koren scheme,showing that the proposed scheme is more efficient.
基金Supported by the National Natural Science Foundation of China(No.31440089)the National Spark Program of China(No.2014GA690259)+2 种基金the National Marine Public Welfare Project of China(No.200905020)the Natural Science Research General Program of Jiangsu Provincial Higher Education Institutions(No.14KJD240002)the Special Guide Fund Project of Agricultural Science and Technology Innovation of Yancheng(No.13KJB610056)
文摘Three color variants of the sea cucumber,Apostichopus japonicus are recognized,the red one is highly valued in the market. When the red variant is cultured in ponds in China,its body color changes from red to celadon in 3–6 months. The effects of water depth and substrate color on the growth and body color of this animal were investigated. Juveniles of red A. japonicus were cultured in cages suspended at a range of water depths(20,50,100,150 and 200 cm). The specific growth rate of red sea cucumbers was significantly higher in animals cultured at deeper water layers compared with those grown at shallowers. Body weights were greatest for sea cucumbers cultured at a depth of 150 cm and their survival rates were highest at a depth of 200 cm. A scale to evaluate the color of red sea cucumbers(R value) was developed using a Pantone standard color card. All stocked animals in the 9-month trial retained a red color,however the red body color was much more intense in sea cucumbers cultured at shallower depths,while animals suspended in deeper layers became pale. In a separate trial,A. japonicus were cultured in suspended cages with seven different colored substrates. Substrate color had a significant effect on the growth and body-color of red A. japonicus. The yield were greatest for A. japonicus cultured on a yellow substrate,followed by green > white > orange > red > black and blue. All sea cucumbers in the 7-month trial retained a red color,although the red was most intense(highest R value) in animals cultured on a blue substrate and pale(lowest R value) for animals cultured on a green substrate.
基金supported by the National Great Project of Scientific and Technical Supporting Programs of China (Grant No. 2011BAD13B03)]the Program for Excellent Youth Foundation of Shandong province (Grant No. JQ201009)the Public Science and Technology Research Funds Projects of Ocean, State Oceanic Administration of the People’s Republic of China (Grant No. 200905020)
文摘Effects of the amplitude(± 2, ± 4, ± 6, and ± 8) and frequency(2, 4, and 8 d) of salinity fluctuation on the body composition and energy budget of juvenile tongue sole(Cynoglossus semilaevis) were investigated in a 64-d experiment. Results showed that the amplitude and frequency of salinity fluctuation had significant interaction and both substantially affected the final weight and specific growth rate of juvenile tongue sole. The tongue sole exhibited better growth in treatments with moderate amplitude and frequency of salinity fluctuation(amplitude ± 4–6; frequency 4–8 d) than in other treatments and the control. In terms of energy budget, salinity fluctuation strongly affected the proportions of energy components, including those deposited for growth and lost in respiration, feces, and excretion. Moderately amplitude and frequency of salinity fluctuationg that favored the growth of tongue sole partitioned more energy for growth and less energy for metabolism than the constant and other amplitude and frequency of salinity fluctuation. Average energy budget for tongue sole at moderately fluctuating salinity was determined to be 100C(food) = 30.92G(growth) + 10.30F(feces) + 6.77U(excretion) + 52.01R(respiration). Energetic advantage at moderately fluctuating salinity, including increased energy intake, high assimilation efficiency, reduced metabolism expenditure, and more energy partitioned into growth, might account for the enhancement of tongue sole growth. Commercial farmers are recommended to rear juvenile tongue sole with moderate salinity fluctuations for better growth performance of this species.
基金Projects(51971040,51701029,51671041,51531002) supported by the National Natural Science Foundation of ChinaProject(2016YFB0301100) supported by the National Key Research and Development Program of China+2 种基金Projects(2018T110943,2017M620410) supported by the China Postdoctoral Science FoundationProject(Xm2017010) supported by the the Chongqing Postdoctoral Scientific Research Foundation,ChinaProject(2018CDGFCL005) supported by the Fundamental Research Funds for the Central Universities,China
文摘Mg-4 Li-3(Al-Si),Mg-8 Li-3(Al-Si)and Mg-12 Li-3(Al-Si)alloys based on theα-Mg,α-Mg+β-Li,β-Li phases,respectively,were produced to investigate the effect of alloying with Al-Si eutectic on the microstructure and mechanical properties of the three alloys.Alloying with the Al-Si eutectic of Mg-xLi(x=4,8 and 12 wt.%)alloys caused the formation of different types of Al-Li precipitates:Al3Li,AlLi and Li3Al2,respectively.Also,considerable quantities of Mg2Si phase particles were found in the three alloys.The results of tensile tests showed that the Mg-4Li-3(Al-Si)alloy exhibited the highest ultimate tensile strength(UTS)of 249 MPa but the lowest elongation of 6.3%.The Mg-12Li-3(Al-Si)alloy had the highest elongation of 26%but the lowest UTS of 173 MPa.The different mechanical properties were attributed to the different crystal structures of the three alloys and the precipitate with different type,morphology and distribution.
基金Supported by the Program for New Century Excellent Talents in University(No.NCET-05-0597)the National Natural Science Foundation of China(No.30270258)
文摘We evaluated the effects of ultraviolet-B (UV-B) radiation and different light conditions on the repair of UV-B-induced damage in carpospores of Chondrus ocellatus Holm (Rhodophyta) in laboratory experiments. Carpospores were treated daily with different doses of UV-B radiation for 48 days, when vertical branches had formed in all treatments; after each daily treatment, the carpospores were subjected to photosynthetically active radiation (PAR), darkness, red light, or blue light during a 2-h repair stage. Carpospore diameters were measured every 4 days. We measured the growth and cellular contents of cyclobutane pyrimidine dimers (CPDs), chlorophyll a, phycoerythrin, and UV-B-absorbing mycosporine-like amino acids (MAAs) in carpospores on Day 48. Low doses of UV-B radiation (36 and 72 J/m2) accelerated the growth of C. ocellatus. However, as the amount of UV-B radiation increased, the growth rate decreased and morphological changes occurred. UV-B radiation significant damaged DNA and photosynthetic pigments and induced three kind of MAAs, palythine, asterina-330, and shinorine. PAR conditions were best for repairing UV-B-induced damage. Darkness promoted the activity of the DNA dark- repair mechanism. Red light enhanced phycoerythrin synthesis but inhibited light repair of DNA. Although blue light, increased the activity of DNA photolyase, greatly improving remediation efficiency, the growth and development of C. ocellatus earpospores were slower than in other light treatments.
基金supported by the National High Technology Research and Development Program of China(863 Program)(Grant Number 2002AA639370).
文摘The effects of the artificial-diversion of the Yellow River Mouth for extensive petroleum exploration beginning in August 1996 on the morphology of the mud flat coast are discussed. Changes in the morphology of the coastal zone were monitored during the period from 1996 to 2000. A Golden Software system was used to calculate the differential surfaces between the surveys in order to investigate the local patterns of accretion and erosion and to determine the volume changes between consecutive surveys. Volumetric studies showed that the net accretion in the study period was about 1.7×10 8 m 3. Between July 1996 and October 1996, a time interval characterized by intense runoff, an accumulation of about 4×10 8 m 3 occurred. In the period from October 1996 to October 1998, a period of diminution in runoff and sediment supply in the upstream, an erosion of 8.9×10 7 m 3 occurred, indicating sediment starvation of the area. Between October 1998 and October 2000, due to an inadequate supply of runoff and sediment, about 1.4×10 8 m 3 of sediments was eroded and transported offshore.
文摘During transcription initiation,RNA polymerase binds tightly to the promoter DNA defining the start of transcription,transcribes comparatively slowly,and frequently releases short transcripts(3-8 nucleotides)in a process called abortive cycling.Transitioning to elongation,the second phase of transcription,the polymerase dissociates from the promoter while RNA synthesis continues.Elongation is characterized by higher rates of transcription and tight binding to the RNA transcript.The RNA polymerase from enterophage T7 (T7 RNAP) has been used as a model to understand the mechanism of transcription in general,and the transition from initiation to elongation specifically.This single-subunit enzyme undergoes dramatic conformational changes during this transition to support the changing requirements of nucleic acid interactions while continuously maintaining polymerase function.Crystal structures,available of multiple stages of the initiation complex and of the elongation complex,combined with biochemical and biophysical data,offer molecular detail of the transition.Some of the crystal structures contain a variant of T7 RNAP where proline 266 is substituted by leucine.This variant shows less abortive products and altered timing of transition,and is a valuable tool to study these processes.The structural transitions from early to late initiation are well understood and are consistent with solution data.The timing of events and the structural intermediates in the transition from late initiation to elongation are less well understood,but the available data allows one to formulate testable models of the transition to guide further research.
基金Supported by TOTAL(DS-2885)the National Natural Science Foundation of China(91434201,21422608)the “Strategic Priority Research Program” of the Chinese Academy of Sciences(XDA07080000)
文摘The cohesive solids in liquid flows are featured by the dynamic growth and breakage of agglomerates, and the difficulties in the development, design and optimization of these systems are related to this significant feature.In this paper, discrete particle method is used to simulate a solid–liquid flow system including millions of cohesive particles, the growth rate and breakage rate of agglomerates are then systematically investigated. It was found that the most probable size of the agglomerates is determined by the balance of growth and breakage of the agglomerates the cross point of the lines of growth rate and breakage rate as a function of the particle numbers in an agglomerate, marks the most stable agglomerate size. The finding here provides a feasible way to quantify the dynamic behaviors of growth and breakage of agglomerates, and therefore offers the possibility of quantifying the effects of agglomerates on the hydrodynamics of fluid flows with cohesive particles.
文摘The study on soil thermal conductivity (STC) was an important side of research on ground source heat pump technique,geological disposal of high-level radioactive wastes,heat distribution of buried cable. Especially owing to technical requirement for shallow terrestrial heat recently, it directly influenced the design and solution in engineering problems. The authors measured the STC in the studied area with QTM-D2 and discussed the effect of samples in size, the measurement error between the samples in lab and in site. The results indicate measuring STC by heat pole method with less influence upon the samples in size, and measuring results on the different geometry size approach very much. The STC is fit for the empirical relation between the temperature and TC under the condition of normal temperature. It is significance for understanding STC in northern China and simulation of temperature field.
基金Supported by a grant from the Science and Technology Plan Projects of Lanzhou(No.2013-3-38)
文摘Objective: The purpose of the study was to investigate the effects of parathyroid hormone and parathyroid hormone receptor monoclonal antibody on in vitro growth and proliferation of human medullary thyroid carcinoma cell lines. Methods: The medullary thyroid carcinoma cell line was cultured in vitro, with parathyroid hormone and parathyroid hormone receptor monoclonal antibody treatment intervention, the growth of the cells was observed under an inverted contrast micro scope, the MTT assay was used to detect the cell growth inhibition rate. Results: Under the inverted contrast microscope, the cells changed significantly, the parathyroid hormone and parathyroid hormone receptor monoclonal antibodies can effectively inhibit the proliferation of medullary thyroid cancer cells in a time and dose dependent. When parathyroid hormone concentra tion reached a concentration of 2.0 IJmol/L, the parathyroid hormone receptor monoclonal antibody reached a concentration of 1.0 μmol/L, the cell growth was most significantly inhibited (P 〈 0.05). Conclusion: Parathyroid hormone and parathyroid hormone receptor monoclonal antibody were able to inhibit the proliferation of medullary thyroid carcinoma cells and signifi cantly reduce the proliferation index.
文摘Objective To investigate fingernail growth rate (FNGR) role in the physiological or pathological status of the fingernails in normal Chinese population. Methods The FNGR was measured with vernier caliper. The data of 1 595 fingernails from 208 normal Chinese subjects (including 96 men and 112 women; age ranging from 14 to 78 years) were analyzed. Results The average FNGR was (0.104±0.027) mm per day. Higher growth rates were observed in males than in females, in the young individuals than in the old individuals, in summer than in winter, and in the right hand than in the left hand, respectively. The FNGR differed among fingernails and decreased in order of precedence: middle fingernails, index fingernails or ring fingernails, thumb and little fingernails. Conclusion FNGR was significantly associated with age, gender and temperature. Different fingernail grew at an individual speed.
文摘in this paper,we prove that a complete n-dimensional Riemannian manifold with nonnegative kth-Ricci curvature, large volume growth has finite topological type provided that lim r→∞{(vol[B(p.r]/ωnrn-αM)rk(n-1)/k+1(1-α/2)}≤for some COllstant ε〉0 We also prove that a conlplete Riemannian manifold with nonnegative kth-Ricci curvature and undler some pinching conditions is diffeomorphic to R^n.