AIM: To explore epigenetic changes in the gene encod- ing X chromosome-linked inhibitor of apoptosis-associ- ated factor 1 (XAF1) during esophageal carcinogenesis. METHODS: Methylation status of XAF1 was detected ...AIM: To explore epigenetic changes in the gene encod- ing X chromosome-linked inhibitor of apoptosis-associ- ated factor 1 (XAF1) during esophageal carcinogenesis. METHODS: Methylation status of XAF1 was detected by methylation-specific polymerase chain reaction (MSP) in four esophageal cancer cell lines (KYSE30, KYSE70, BICl and partially methylated in TE3 cell lines), nine cases of normal mucosa, 72 cases of pri- mary esophageal cancer and matched adjacent tissue. XAF1 expression was examined by semi-quantitative reverse transcriptional polymerase chain reaction and Western blotting before and after treatment with 5-aza- deoxycytidine (5-aza-dc), a demethylating agent. To investigate the correlation of XAF1 expression and methylation status in primary esophageal cancer, immu- nohistochemistry for XAF1 expression was performed in 32 cases of esophageal cancer and matched adjacent tissue. The association of methylation status and clini-copathological data was analyzed by logistic regression. RESULTS: MSP results were as follows: loss of XAF1 expression was found in three of four esophageal cell lines with promoter region hypermethylation (com- pletely methylated in KYSE30, KYSE70 and BIC1 cell lines and partially in TE3 cells); all nine cases of normal esophageal mucosa were unmethylated; and 54/72 (75.00%) samples from patients with esophageal can- cer were methylated, and 25/72 (34.70%) matched adjacent tissues were methylated (75.00% vs 34,70%, z2 = 23.5840, P = 0.000). mRNA level of XAF1 mea- sured with semi-quantitative reverse transcription poly- merase chain reaction was detectable only in TE3 cells, and no expression was detected in KYSE30, KYSE70 or BIC1 cells. Protein expression was not observed in KYSE30 cells by Western blotting before treatment with 5-aza-dc. After treatment, mRNA level of XAF1 was detectable in KYSE30, KYSE70 and BIC1 cells. Protein expression was detected in KYSE30 after treatment with 5-aza-dc. Immunohistochemistry was performed on 32 cases of esophageal cancer and adjacent tissue, and demonstrated XAF1 in the nucleus and cytoplasm. XAF1 staining was found in 20/32 samples of adjacent normal tissue but was present in only 8/32 samples of esophageal cancer tissue (Z2= 9.143, P = 0.002). XAF1 expression was decreased in cancer samples compared with adjacent tissues. In 32 cases of esophageal can- cer, 24/32 samples were methylated, and 8/32 esopha- geal cancer tissues were unmethylated. XAF1 staining was found in 6/8 samples of unmethylated esophageal cancer and 2/24 samples of methylated esophageal cancer tissue. XAF1 staining was inversely correlated with XAF1 promoter region methylation (Fisher's exact test, P = 0.004). Regarding methylation status and clinicopathological data, no significant differences were found in sex, age, tumor size, tumor stage, or metas- tasis with respect to methylation of XAF1 for the 72 tis- sue samples from patients with esophageal cancer. CONCLUSION: XAF1 is frequently methylated in eso- phageal cancer, and XAF1 expression is regulated by promoter region hypermethylation.展开更多
The Preston's method is considered as one of the most commonly employed methods to measure the wall shear stress. However, it is only possible to determine the wall shear stress from measured pressure differences of ...The Preston's method is considered as one of the most commonly employed methods to measure the wall shear stress. However, it is only possible to determine the wall shear stress from measured pressure differences of the Preston tube and undisturbed static pressure, combined with calibration curves, which depend on the Preston tube diameter, fluid density, and viscosity. Since its invention, no significant advancement in theory has been made, and calibration curves proposed by Preston, Patel and Bechert are still in use. In the present study, a need to measure surface shear stress over a circular cylinder prompted us to develop our original Preston tube system. The developed system has been calibrated by measuring the wall shear stress in the fully developed turbulent flow regime in a circular pipe. The present results generally confirm the previously reported calibration curves. A slight modification of the coefficients in the calibration equation shows further improvement.展开更多
In this report, the author describes and compares two innovative processes for producing thermal energy based on cavitation and nuclear fusion reactions in the indoor environment. Experiments conducted in the laborato...In this report, the author describes and compares two innovative processes for producing thermal energy based on cavitation and nuclear fusion reactions in the indoor environment. Experiments conducted in the laboratory IHS (Intensive Heating System) of IE "Revinov N. M." indicate that the process of lasso-vortex cavitation, which generates by means of an electric arc obtained HV-EI, gas-liquid plasma state in the EHH-CTC (electro-hydraulic heater with cavitation thermal camera) is not inferior and sometimes even superior in heat transfer to NF-AC (nuclear fusion reactions in ambient conditions).展开更多
The work presents the results of tests on the shear parameters of walls made of AAC (autoclaved aerated concrete, fb = 4.0 N/mm2) on the system mortar for thin M5 and M10 joints (fm = 6.1 N/mm2 and fro = 11.9 N/mm2...The work presents the results of tests on the shear parameters of walls made of AAC (autoclaved aerated concrete, fb = 4.0 N/mm2) on the system mortar for thin M5 and M10 joints (fm = 6.1 N/mm2 and fro = 11.9 N/mm2) and on polyurethane glue and also walls without mortar (dry masonry). The wall compression strength (on mortar M5 class) (per EN 1052-1:2000) amounted tofc,mv= 2.97 N/mm2 (fk = 2.48 N/mm2), elastic modulus was Ecru = 2,040 N/mm2. Various structure of bed joints and head joints were applied and the following were used as reinforcement: steel trusses of EFZ 140/Z 140 type (Z1 type) and meshes made of plastics (Z2 type). Based on the tests carried out with regard to unreinforced elements, it was shown that the filling in of head joints with mortar had an advantageous effect on the values of cracking and destruction stresses. While, with the use of reinforcement, advantageous increase of stress was obtained only when the mortar was laid twice on both bed surfaces of masonry units. The application of reinforcement in the bed joints when the mortar was laid only on one bed joints surface of the masonry units reduced the values of cracking and destruction stresses in relation to the values obtained in the unreinforced walls.展开更多
Uranium tetrafluoride, UF4, and thorium tetrafluoride, ThF4, can be used as fuels in molten salt reactors. For the molten salt reactor design and safety analysis, it is essential to know the thermodynamic properties o...Uranium tetrafluoride, UF4, and thorium tetrafluoride, ThF4, can be used as fuels in molten salt reactors. For the molten salt reactor design and safety analysis, it is essential to know the thermodynamic properties of the UF4 and ThF4 materials. However, the experimental data for UF4 and ThF4 P-V-T are scarce in literature. Under this circumstance prediction of the thermodynamic properties can be supported by theoretical calculations to remedy missing experimental data. Within this paper the Song, Mason and lhm's equation of state with modification of Tao and Mason, originally derived for spherical and molecular fluids, is applied for fluid UF4 and ThF4 based on the available experimental data. The equation of state is based on statistical mechanical perturbation theory with the perturbation scheme of Weeks, Chandler, and Andresen. The prediction of constants applied in the equation of state is based on the work of Boushehri et al. using data for heat of vaporization and liquid density at the triple point. The calculation of the heat of vaporization applies the "sigma" method reported by Darken et al. with the vapour pressure data and heat capacities of liquid and vapour of UF4 and ThF4. Finally an extra correction term for the vapour pressure is introduced into the new equation of state. The results show that this equation of state agrees reasonably well with the available experimental data. It can be expected that this equation of state can be applied also for conditions where experimental data are currently missing.展开更多
基金Supported by Grants from the National Basic Research Program(973 Program), No. 2012CB934002, 2010CB912802National Key Scientific Instrument Special Programme of China, No.2011YQ03013405National Science Foundation of China,No. 81121004, 81071953 and 81161120432
文摘AIM: To explore epigenetic changes in the gene encod- ing X chromosome-linked inhibitor of apoptosis-associ- ated factor 1 (XAF1) during esophageal carcinogenesis. METHODS: Methylation status of XAF1 was detected by methylation-specific polymerase chain reaction (MSP) in four esophageal cancer cell lines (KYSE30, KYSE70, BICl and partially methylated in TE3 cell lines), nine cases of normal mucosa, 72 cases of pri- mary esophageal cancer and matched adjacent tissue. XAF1 expression was examined by semi-quantitative reverse transcriptional polymerase chain reaction and Western blotting before and after treatment with 5-aza- deoxycytidine (5-aza-dc), a demethylating agent. To investigate the correlation of XAF1 expression and methylation status in primary esophageal cancer, immu- nohistochemistry for XAF1 expression was performed in 32 cases of esophageal cancer and matched adjacent tissue. The association of methylation status and clini-copathological data was analyzed by logistic regression. RESULTS: MSP results were as follows: loss of XAF1 expression was found in three of four esophageal cell lines with promoter region hypermethylation (com- pletely methylated in KYSE30, KYSE70 and BIC1 cell lines and partially in TE3 cells); all nine cases of normal esophageal mucosa were unmethylated; and 54/72 (75.00%) samples from patients with esophageal can- cer were methylated, and 25/72 (34.70%) matched adjacent tissues were methylated (75.00% vs 34,70%, z2 = 23.5840, P = 0.000). mRNA level of XAF1 mea- sured with semi-quantitative reverse transcription poly- merase chain reaction was detectable only in TE3 cells, and no expression was detected in KYSE30, KYSE70 or BIC1 cells. Protein expression was not observed in KYSE30 cells by Western blotting before treatment with 5-aza-dc. After treatment, mRNA level of XAF1 was detectable in KYSE30, KYSE70 and BIC1 cells. Protein expression was detected in KYSE30 after treatment with 5-aza-dc. Immunohistochemistry was performed on 32 cases of esophageal cancer and adjacent tissue, and demonstrated XAF1 in the nucleus and cytoplasm. XAF1 staining was found in 20/32 samples of adjacent normal tissue but was present in only 8/32 samples of esophageal cancer tissue (Z2= 9.143, P = 0.002). XAF1 expression was decreased in cancer samples compared with adjacent tissues. In 32 cases of esophageal can- cer, 24/32 samples were methylated, and 8/32 esopha- geal cancer tissues were unmethylated. XAF1 staining was found in 6/8 samples of unmethylated esophageal cancer and 2/24 samples of methylated esophageal cancer tissue. XAF1 staining was inversely correlated with XAF1 promoter region methylation (Fisher's exact test, P = 0.004). Regarding methylation status and clinicopathological data, no significant differences were found in sex, age, tumor size, tumor stage, or metas- tasis with respect to methylation of XAF1 for the 72 tis- sue samples from patients with esophageal cancer. CONCLUSION: XAF1 is frequently methylated in eso- phageal cancer, and XAF1 expression is regulated by promoter region hypermethylation.
文摘The Preston's method is considered as one of the most commonly employed methods to measure the wall shear stress. However, it is only possible to determine the wall shear stress from measured pressure differences of the Preston tube and undisturbed static pressure, combined with calibration curves, which depend on the Preston tube diameter, fluid density, and viscosity. Since its invention, no significant advancement in theory has been made, and calibration curves proposed by Preston, Patel and Bechert are still in use. In the present study, a need to measure surface shear stress over a circular cylinder prompted us to develop our original Preston tube system. The developed system has been calibrated by measuring the wall shear stress in the fully developed turbulent flow regime in a circular pipe. The present results generally confirm the previously reported calibration curves. A slight modification of the coefficients in the calibration equation shows further improvement.
文摘In this report, the author describes and compares two innovative processes for producing thermal energy based on cavitation and nuclear fusion reactions in the indoor environment. Experiments conducted in the laboratory IHS (Intensive Heating System) of IE "Revinov N. M." indicate that the process of lasso-vortex cavitation, which generates by means of an electric arc obtained HV-EI, gas-liquid plasma state in the EHH-CTC (electro-hydraulic heater with cavitation thermal camera) is not inferior and sometimes even superior in heat transfer to NF-AC (nuclear fusion reactions in ambient conditions).
文摘The work presents the results of tests on the shear parameters of walls made of AAC (autoclaved aerated concrete, fb = 4.0 N/mm2) on the system mortar for thin M5 and M10 joints (fm = 6.1 N/mm2 and fro = 11.9 N/mm2) and on polyurethane glue and also walls without mortar (dry masonry). The wall compression strength (on mortar M5 class) (per EN 1052-1:2000) amounted tofc,mv= 2.97 N/mm2 (fk = 2.48 N/mm2), elastic modulus was Ecru = 2,040 N/mm2. Various structure of bed joints and head joints were applied and the following were used as reinforcement: steel trusses of EFZ 140/Z 140 type (Z1 type) and meshes made of plastics (Z2 type). Based on the tests carried out with regard to unreinforced elements, it was shown that the filling in of head joints with mortar had an advantageous effect on the values of cracking and destruction stresses. While, with the use of reinforcement, advantageous increase of stress was obtained only when the mortar was laid twice on both bed surfaces of masonry units. The application of reinforcement in the bed joints when the mortar was laid only on one bed joints surface of the masonry units reduced the values of cracking and destruction stresses in relation to the values obtained in the unreinforced walls.
文摘Uranium tetrafluoride, UF4, and thorium tetrafluoride, ThF4, can be used as fuels in molten salt reactors. For the molten salt reactor design and safety analysis, it is essential to know the thermodynamic properties of the UF4 and ThF4 materials. However, the experimental data for UF4 and ThF4 P-V-T are scarce in literature. Under this circumstance prediction of the thermodynamic properties can be supported by theoretical calculations to remedy missing experimental data. Within this paper the Song, Mason and lhm's equation of state with modification of Tao and Mason, originally derived for spherical and molecular fluids, is applied for fluid UF4 and ThF4 based on the available experimental data. The equation of state is based on statistical mechanical perturbation theory with the perturbation scheme of Weeks, Chandler, and Andresen. The prediction of constants applied in the equation of state is based on the work of Boushehri et al. using data for heat of vaporization and liquid density at the triple point. The calculation of the heat of vaporization applies the "sigma" method reported by Darken et al. with the vapour pressure data and heat capacities of liquid and vapour of UF4 and ThF4. Finally an extra correction term for the vapour pressure is introduced into the new equation of state. The results show that this equation of state agrees reasonably well with the available experimental data. It can be expected that this equation of state can be applied also for conditions where experimental data are currently missing.