基于生本体验,构建学科观念——“电解池的工作原理及应用”教学设计与思考

以苏教版选修教材《化学反应原理》第二单元中的“电解池的工作原理及应用”一节为例,探讨在教学中如何基于学生体验，有效渗透化学微粒观、实验观、化学价值观的问题.

护生《职业体验》课中引入人文关怀护理观的效果探讨

目的对护生《职业体验》课中人文关怀护理观的引入效果进行探讨。方法选取该院2018年10—11月共99名职业体验护生进行研究,成立活动小组,从理论讲座、案例分析、情境演练等方面将人文关怀护理观传授给职业体验护生,在护生护理操作过程中带教老师间接将人文关怀护理观引入,对职业体验后护生对护理关怀行为认知评分及护生对带教老师的关怀能力评分进行分析。结果护生对护理关怀行为的认识处于中等偏上水平,护生对带教老师的关怀能力的评分也处于中等偏上水平,两项指标得分等级存在正相关,差异有统计学意义(P<0.000 1)。结论在护生《职业体验》课中,将人文关怀护理引入带教中,可提高护生对护理关怀行为的认知水平,及提高综合素质提升,对学生未来的学习和工作有促进作用。

自主体验感受 探究知识脉络——人教版数学四年级上册《复式条形统计图》教学设计

教材分析:学生在第一学段已经初步体验了数据的收集、整理、描述和分析的过程,会用简单的统计图表表示统计的结果,能够根据统计图表提出一些简单的问题,初步经历了用统计的方法解决问题的过程,了解了统计在现实生活中的作用和意义,并初步建立统计观念。

写作从观察开始

教了十几年语文后,我发现写作文是一件令师生都十分头疼的事情。学生头疼是因为他们觉得无啥可写,无啥好写,教师命题后,学生只有望题兴叹,搜索枯肠,勉强成文,应付了事。教师面对这种"闭门造车"式的文章,自然也是头疼不已。

Method of Growth in Finding the Test Cube of Industrial Robots

In order to find the test cube for industrial robots as specified by ISO 9283, a seed cube is grown up in an irregular working space of the robot, provided that the corners of the cube do not exceed the boundary of the working space. All possible cubes are searched, and the cube with the maximum volume is selected. The calculation examples show that the method of growth can be used for a variety of industrial robots. The method of growth can determine the test cube and test points of irregular working space according to ISO 9283, and can avoid blindness and randomness in the selection of test points.

谈数学教学中的德育渗透

一充分挖掘教材中的德育内容,对学生进行爱国主义教育随着信息技术的迅速发展,可供教育教学利用的资源越来越丰富了。这些多彩的网络资源,教师可以选择富有教育意义的、形象生动的插图,有说服力的数据和统计材料。

PI3K/Akt/mTOR inhibitors in breast cancer

Activation of the phosphoinositide 3 kinase(PI3K)/Akt/mammalian target of rapamycin(mTOR) pathway is common in breast cancer. There is preclinical data to support inhibition of the pathway, and phase Ⅰ to Ⅲ trials involving inhibitors of the pathway have been or are being conducted in solid tumors and breast cancer. Everolimus, an mTOR inhibitor, is currently approved for the treatment of hormone receptor(HR)-positive, human epidermal growth factor receptor 2(HER2)-negative breast cancer. In this review, we summarise the efficacy and toxicity findings from the randomised clinical trials, with simplified guidelines on the management of potential adverse effects. Education of healthcare professionals and patients is critical for safety and compliance. While there is some clinical evidence of activity of mTOR inhibition in HR-positive and HER2-positive breast cancers, the benefits may be more pronounced in selected subsets rather than in the overall population. Further development of predictive biomarkers will be useful in the selection of patients who will benefit from inhibition of the PI3K/Akt/mTOR(PAM) pathway.

Control of combustion area using electrical resistivity method for underground coal gasification

Underground coal gasification （UCG） is one of the clean technologies to collect heat energy and gases （hydrogen, methane, etc.） in an underground coal seam. It is necessary to further developing environ- mentally friendly UCG system construction. One of the most important UCG＇s problems is underground control of combustion area for efficient gas production, estimation of subsidence and gas leakage to the surface. For this objective, laboratory experiments were conducted according to the UCG model to iden- ti[y the process of combustion cavity development by monitoring the electrical resistivity activity on the coal samples to setup fundamental data for the technology engineering to evaluate combustion area. While burning coal specimens, that had been sampled from various coal deposits, electrical resistivity was monitored. Symmetric four electrodes system （ABMN） of direct and low-frequency current electric resistance method was used for laboratory resistivity measurement of rock samples. Made research and the results suggest that front-end of electro conductivity activity during heating and combusting of coal specimen depended on heating temperature. Combusting coal electro conductivity has compli- cated multistage type of change. Electrical resistivity method is expected to be a useful geophysical tool to for evaluation of combustion volume and its migration in the coal seam.

Molecular Mechanisms Contributing to Resistance to Tyrosine Kinase-Targeted Therapy for Non-Small Cell Lung Cancer

One of the most important pathways in non-small cell lung cancer(NSCLC) is the epidermal growth factor receptor(EGFR) pathway. This pathway affects several crucial processes in tumor development and progression,including tumor cell proliferation,apoptosis regulation,angiogenesis,and metastatic invasion.Targeting EGFR is currently being intensely explored.We are witnessing the development of a number of potential molecular-inhibiting treatments for application in clinical oncology.In the last decade,the tyrosine kinase(TK) domain of the EGFR was identified in NSCLC patients,and it has responded very well with a dramatic clinical improvement to TK inhibitors such are gefitinib and erlotinib.Unfortunately,there were primary and/or secondary resistance to these treatments,as shown by clinical trials.Subsequent molecular biology studies provided some explanations for the drug resistance phenomenon.The molecular mechanisms of resistance need to be clarified.An in-depth understanding of these targeted-therapy resistance may help us explore new strategies for overcoming or reversing the resistance to these inhibitors for the future of NSCLC treatment.

游戏精神:音乐课堂亟待回归的风景线——音乐游戏在张力课堂中的运用初探

在充满张力的音乐课堂中,我们融入音乐性特点的游戏,把它作为一种载体,在音乐游戏中强调学生的主体性地位,知晓音乐要素,感受音乐情绪,激发音乐的创造力,真正地享受音乐。

In Silico Screening of Potential Drug with Antileishmanial Activty and Validation of their Activity by in Vitro and in Vivo Studies

The research on discovery and development of new treatments for cutaneous leishmaniasis has been declared as priority. Using bioinformatics approaches, this study aimed to identify antileishmanial activity in drugs that are currently used as anti-inflammatory and wound healing by such anti-Leishmania activity was validated by in vitro and in vivo assays. In silico analysis identified 153 compounds from which 87 were selected by data mining of DrugBank database, 22 and 44 were detected by PASS （http：//pass.cribi.unipd.it） and BLAST （http：//blast.ncbi.nlm.nih. gov/） alignment, respectively. The majority of identified drugs are used as skin protector, anti-acne, anti-ulcerative （wound healer） or anti-inflammatory and few of them had specific antileishmanial activity. The efficacy as antileishmanial was validated in vitro in 12/23 tested compounds and in all seven compounds that were evaluated in in vivo assays. Notably, this is the first report of antileishmanial activity for adapalene. In conclusion, bioinformatics tools not only can help to reduce time and cost of the drug discovery process but also may increase the chance that candidates identified in silico which have a validated antileishmanial activity by combining different biological properties.

An Experimental Biotelemetric Study Based on Satellite Tracking During Post-nesting Migrations of Green Turtles

A biotelemetry experiment is conducted to study the migrant behavior of green turtles (Chelonia mydas) in South China Sea and acquire the physical environment data. The method in use is to track the post nesting migrant routes of green turtles through the satellite linked transmitters attached on the back of turtles and the global satellite signal transmitting system of Argos. We obtained the positions of the post nesting migrant routes of three green turtles and environment data, which are important in conservation of green turtles and the research of physical oceanography. Based on the test, the concept, principle and method of biotelemetry are also introduced in this paper with a discussion of the further development of this technique and its applying prospection in future.

Gastric stimulation for weight loss

The prevalence of obesity is growing to epidemic proportions,and there is clearly a need for minimally invasive therapies with few adverse effects that allow for sustained weight loss.Behavior and lifestyle therapy are safe treatments for obesity in the short term,but the durability of the weight loss is limited.Although promising obesity drugs are in development,the currently available drugs lack efficacy or have unacceptable side effects.Surgery leads to long-term weight loss,but it is associated with morbidity and mortality.Gastric electrical stimulation(GES) has received increasing attention as a potential tool for treating obesity and gastrointestinal dysmotility disorders.GES is a promising,minimally invasive,safe,and effective method for treating obesity.External gastric pacing is aimed at alteration of the motility of the gastrointestinal tract in a way that will alter absorption due to alteration of transit time.In addition,data from animal models and preliminary data from human trials suggest a role for the gut-brain axis in the mechanism of GES.This may involve alteration of secretion of hormones associated with hunger or satiety.Patient selection for gastric stimulation therapy seems to be an important determinant of the treatment's outcome.Here,we review the current status,potential mechanisms of action,and possible future applications of gastric stimulation for obesity.

Analysis of Acoustic Emission Signals Accompanying Growth of Single Aluminum Crystals： Experimental Results and Theoretical Model of the Cluster

The purpose of the work is to identify the acoustic emission （AE） signal in the melt and from the interphase during the crystal growth and to establish the connection between issue parameters： the number of signal events of frequency and the signal power with the growth conditions of temperature gradient and crystallization rate. Experiments on single crystal growth were carried out using hardware and software system which allows to perform spectral Fourier analysis of AE signals and to simultaneously remove the cooling curve for the entire period of crystallization. On the basis of spectral analysis of AE signals, a theoretical model of clusters in the aluminum melt was designed. The experimental results indicate an uneven abrupt advancement of the interface according to the configuration of each individual cluster.

Optimization for Production of Intracellular Polysaccharide from Cordyceps ophioglossoides L2 in Submerged Culture and Its Antioxidant Activities in vitro

Cordyceps ophioglossoides is a valuable traditional medicinal material.We have found that intracellular polysaccharide(IPS) is the major biologically active ingredient in Cordyceps ophioglossoides.This study is the first time to optimize the yield of IPS from Cordyceps ophioglossoides.The optimal medium for IPS production consists of glucose 54.50 g·L·1,yeast powder 25.50 g·L·1,NaH2PO4 0.4 g·L·1 and K2HPO4 0.4 g·L·1.The suggested culture conditions are 24 ℃,initial pH 4.5 with a rotary speed of 120 r·min·1 for 168 h.The yield of IPS is 737.93 mg·L·1,which is 50% higher than the yield under the conditions prior to optimization.The anti-oxidative activities of IPS in Cordyceps ophioglossoides L2 are also characterized using various in vitro assay.The anti-oxidative activity may explain the reason why IPS from Cordyceps ophioglossoides can be used to fight against neurodegenerative dis-eases and menopausal symptoms.

In-vitro and In-vivo Anti-trypanosomal Activity of Terminalia chebula Retz Dried Fruits

African trypanosomosis had caused lots of havocs to both humans and animals over a century with successes and failure in curtailing it. This study was aimed at screening medicinal plant, Terminalia chebula dried fruits against Trypanosoma evansi for trypanocidal activity. Twenty grams of powdered Terminalia chebula dried fruits was cold extracted with methanol. Obtained MPE （methanolic plant extract） was in vitro tested against Trypanosoma brucei （1 × 10^6 trypanosomes/mL of the medium in each ELISA plate wells） at concentrations （250～1,000 μg/mL） on Vero cells grown in DMEM （Debecco＇s Modified Eagle Medium） in appropriate conditions for trypanocidal activity. In-vitro cytotoxicity test of MPE of Terminalia chebula was conducted on Vero cells grown in DMEM. In-vivo assay for trypanocidal activity, each mouse was inoculated with 1 × 10^4/mL of trypanosomes and treated （48 h post inoculation） with MPE of Terminalia chebula at concentrations （12.5, 25, 50, 100 and 200 mg/kg body weight） were administered at dose rate of 100 BL per mouse via intraperitoneal route to different groups of mice, 6 mice per concentration. In-vitro cytotoxicity test was done on Veto cells at concentrations （1.58～100 μg/mL） of MPE of Terminalia chebula. Results of in-vitro trypanocidal activity varied from immobilization, reduction and to the killing of the trypanosomes. At 250 μg/mL ofMPE ofTerminalia chebula dried fruits, there was significant trypanocidal activity at 4 h of incubation and trypanosomes were not detected in corresponding ELISA plate wells at 5 h of incubation, which was statistically equivalent to reference drug, diminazine aceturate （50 μL/mL） at 4 h of incubation. Results of in-vivo trypanocidal activity revealed that at concentrations （l 2.5～25 mg/kg body weight） of MPE of Terrninalia chebula, mice in these groups survived for 6 days. While at 50 and 100 to 200 mg/kg body weight, mice in these groups survived up to 7 and 8 days, respectively. In-vitro cytotoxicity test showed that all concentrations of MPE of Terminalia chenula and diminazine aceturate were cytotoxic to cells except at 1.56 μL/mL and 6.25 μL/mL. In conclusion, MPE of Terminalia chebula dried fruits possessed trypanocidal compounds. Further study （bioassay-guided purification） is required to know the full potential of Terrninalia chebula as future trypanocide candidate.

Determination of Genistein in the Fermented By-product of Soybean Curd, an Indonesian Food, and Its in vivo Assay on Carrageenan-lnduced Mice

In our country, Indonesia, especially in West Java, people frequently consume fermented by-product of soybean curd for their main course. The by-product of curd, usually is discarded, can be fermented using either Neurospora （Neurospora sitophila） or Rhizopus （Rhizopus oligosporus） yeast to make oncom Genistein is a phytoestrogen, an estrogen-like chemical compound present in plants, that has anti-inflammatory property. Genistein can be determined by UV spectrophotometric method based on the UV absorbance of its complex with aluminum chloride. Addition of acid is a critical point in the procedure. The aim of this research was to know whether genistein is still contained in oncom This research also studied about oncom＇s activity on carrageenan-induced mice. Acetosal was used as drug control. Result showed that genistein was detected contained in oncom extract. The in vivo assay indicated that oncom extract （dose 1,000 mg/kg of body weight） reduced oedema in carragenan-induced paw of mice, which potencies were 066 times lower than that of acetosal＇s. Oncom might be useful as food and as a weak inflammation reducer as well.

Comparison of RFFIT Tests with Different Standard Sera and Testing Procedures

The World Health Organization （WHO） standard assay for determining antibody level is the rapid fluorescent focus inhibition test （RFFIT） and is used to determine the degree of immunity after vaccination against rabies. To compare the difference in RFFIT results between the laboratories of The National Institute of Infectious Disease in Japan （NIID） and the Chinese Centre for Disease Control （CCDC） as well the influence of the choice of standard serum （STD） for the detection, the two laboratories detection methods were simultaneously manipulated by RFFIT. The reference serums used in NIID and the WHO standard serum used in CCDC were compared in the same RFFIT detection to determine the titer of four sera samples C1, Sl, S2 and S4 in parallel, and the titers of the detected sera samples were calculated using the standard formula for neutralizing antibody titer. No significant difference was found in RFFIT methods from the two laboratories and the RFFIT testing procedures of the two laboratories have good consistency. However, different titers were obtained with the tentative internal standard serum （TI-STD） produced by adjusting to 2.0 IU of WHO standard serum in NIID and the WHO STD. The titer determined with the TI-STD was higher than that determined with WHO STD, This difference appears to be significant and requires further investigation

Survey on Parasites in Sparrows of Amol （Mazandaran Province, Iran）

In order to survey on sparrows parasites （Passer domesticus）, 100 samples were collected from the citrus gardens of Amol＇s City. The surface of their body checked for presence of ectoparasites. The blood samples took from their wings for blood parasites. At the laboratory, the body of sparrows opened and the gastrointestinal checked for endoparasites. The intestine opened and scratched carefully to find worms. Worms kept in alcohol-glycerin （10%）. Eggs ＆ oocysts of the parasites checked, by fecal floatation test. The result shows that the infection with Railletina is 1%, Ascaridia galli （2%）, Heterakis gallinarum （4%）, eggs of A. galli （5%）, oocyst of Eimeri （28%）, Dermanyssus gallinae （1%）. 11% of the sparrows infected with Plasmodium in their bloods.

LOW MOLECULAR WEIGHT HEPARIN ENHANCES THE EFFECT OF aFGF IN ACCELERATING NEOVASCULA-RIZATION

Objective To explore the potential of low molecular weight heparin (LMWH) in combination cooperated with aFGF in accelerating neovascularization in vivo. Methods Ischemic model was set up in the right hindlimbs of 28 New Zealand white rabbits. Four groups of animals treated with saline, LMWH, aFGF and aFGF plus LMWH were allocated equally in group Ⅰ, group Ⅱ, group Ⅲ and group Ⅳ respectively. Vascular neovascularization and smooth muscular thickness of the ischemic hindlimb vessels of each animal in different groups were compared with each other on the 28th day postoperatively by angiography with DSA and the standard immunoperoxidase technique. Results No significant neovascularization was seen when aFGF adiministered in low dosage by venous infusion. But when the same dosage of aFGF plus LMWH were administered by venous infusion, a significant neovascularization was observed. Conclusion LMWH can potentiate aFGF in accelerating neovascularization.