AIM Toll like receptors plays a significant anti-viral role in different infections. The aim of this study was to look into the role of toll like receptor 4(TLR4) in hepatitis B virus(HBV) infection.METHODS Real time ...AIM Toll like receptors plays a significant anti-viral role in different infections. The aim of this study was to look into the role of toll like receptor 4(TLR4) in hepatitis B virus(HBV) infection.METHODS Real time PCR was used to analyze the transcription of TLR4 signaling molecules, cell cycle regulators and HBV DNA viral load after triggering the Hep G2.2.15 cells with TLR4 specific ligand. Nuclear factor(NF)-κB translocation on TLR4 activation was analyzed using microscopic techniques. Protein and cell cycle analysis was done using Western Blot and FACS respectively.RESULTS The present study shows that TLR4 activation represses HBV infection. As a result of HBV suppression, there are several changes in host factors which include partial release in G1/S cell cycle arrest and changes in host epigenetic marks. Finally, it was observed that anti-viral action of TLR4 takes place through the NF-κB pathway.CONCLUSION The study shows that TLR4 activation in HBV infection brings about changes in hepatocyte microenvironment and can be used for developing a promising therapeutic target in future.展开更多
Experiments and analyses are carried out for GEO and joint GEO/IGSO precise orbit determination using data recorded by China's regional tracking network.Results show that joint GEO/IGSO orbit determination effecti...Experiments and analyses are carried out for GEO and joint GEO/IGSO precise orbit determination using data recorded by China's regional tracking network.Results show that joint GEO/IGSO orbit determination effectively solves the problem of poor observation geometry for GEO satellites.The laser radial evaluation thus confirms that precision is as good as less than 0.1 m.In the case of joint orbit determination,solving the empirical acceleration can reduce errors introduced by the imprecise solar radiation pressure model used for Chinese satellites.This method also improves the accuracy of orbit prediction in the radial direction.The ephemeris accuracy is thus improved and the ephemeris can provide a better service to users with navigation and positioning requirements.展开更多
基金Supported by the Indian Council of Medical Research(ICMR)intramural projectDas C acknowledges the financial support from Biomolecular Assembly,Recognition and Dynamics (BARD) project(Grant 12-R&D-SIN-5.04-0103)from Department of Atomic Energy(DAE),Government of India and Ramalingaswami Fellowship,Department of Biotechnology,Government of India
文摘AIM Toll like receptors plays a significant anti-viral role in different infections. The aim of this study was to look into the role of toll like receptor 4(TLR4) in hepatitis B virus(HBV) infection.METHODS Real time PCR was used to analyze the transcription of TLR4 signaling molecules, cell cycle regulators and HBV DNA viral load after triggering the Hep G2.2.15 cells with TLR4 specific ligand. Nuclear factor(NF)-κB translocation on TLR4 activation was analyzed using microscopic techniques. Protein and cell cycle analysis was done using Western Blot and FACS respectively.RESULTS The present study shows that TLR4 activation represses HBV infection. As a result of HBV suppression, there are several changes in host factors which include partial release in G1/S cell cycle arrest and changes in host epigenetic marks. Finally, it was observed that anti-viral action of TLR4 takes place through the NF-κB pathway.CONCLUSION The study shows that TLR4 activation in HBV infection brings about changes in hepatocyte microenvironment and can be used for developing a promising therapeutic target in future.
基金supported by the National Natural Science Foundation of China (Grant No.41074020)
文摘Experiments and analyses are carried out for GEO and joint GEO/IGSO precise orbit determination using data recorded by China's regional tracking network.Results show that joint GEO/IGSO orbit determination effectively solves the problem of poor observation geometry for GEO satellites.The laser radial evaluation thus confirms that precision is as good as less than 0.1 m.In the case of joint orbit determination,solving the empirical acceleration can reduce errors introduced by the imprecise solar radiation pressure model used for Chinese satellites.This method also improves the accuracy of orbit prediction in the radial direction.The ephemeris accuracy is thus improved and the ephemeris can provide a better service to users with navigation and positioning requirements.
