以往的研究证明由左心房逆行灌注冲洗肺,在短期肺保存中获得良好效果。但此法是否适用于长时间的肺保存,至今尚无资料。也有资料表明:由于肺静脉端较低的血管阻力,常规用于顺行肺灌注—肺保存中的血管扩张素一前列腺素E_1可能不必用于...以往的研究证明由左心房逆行灌注冲洗肺,在短期肺保存中获得良好效果。但此法是否适用于长时间的肺保存,至今尚无资料。也有资料表明:由于肺静脉端较低的血管阻力,常规用于顺行肺灌注—肺保存中的血管扩张素一前列腺素E_1可能不必用于逆行灌注—肺保存。本研究旨在估价逆行灌注和前列腺素E_1(PGE_1)在24h肺保存中的作用。实验狗分为两组,University of Wisconsin保存液(UW液)作为冲洗和保存液。在A组(n=7),250μg PGE_1在进行灌注之前注入供体的肺动脉,另外250μg混合在UW液中。B组为对照组,不用PGE_1。在4℃下保存22～25h后,作同种异体左肺移植。在肺移植之前和之后的10—,30—,60—,120—min测试血流动力学指标、血气分析、呼吸道阻力和肺顺应性。结果表明,经120 min的在体再灌注后,氧和二氧化碳分压(kPa)分别为85.52±3.20和4.93±0.40(A组);79.80±3.87和4.93±0.40(B组)。血流动力学指标(kPa,A组对B组):肺动脉压2.67±0.13对3.73±0.27(P<0.05);右房压0.53±0.13对1.07±0.13(P<0.05);肺血管阻力(dyne·s·cm^(-5))1109±51对1525±133(P<0.05)。气道阻力(kPa/L·s)2.16±0.10对2.35±0.10(P=NS);肺顺应性(CC╱cm)30±1对26±1(P<0.05)。在B组,经2h的再灌注后,气道阻力显著高于实验前的基值(19±1)(P<0.05)。电子显微镜观察表明:除组织间隙水肿外,Ⅰ型肺上皮细胞、毛细血管内皮和微细支气管上皮均现正常。Ⅱ型肺上皮细胞的板层小体内容物减少或消失。上述改变在两组间无明显差别。结论:用UW液逆灌保存犬肺24h是可行的。PGE_1有助于减小肺血管阻力、气道阻力和改善肺顺应性。展开更多
An experiment was conducted to find the effect of three types of donor plants growing conditions （growth chamber, open space ＋ growth chambers and open space） on callus induction and subsequent plant regeneration a...An experiment was conducted to find the effect of three types of donor plants growing conditions （growth chamber, open space ＋ growth chambers and open space） on callus induction and subsequent plant regeneration and productive shoot regeneration from the anthers culture in vitro of four rice hybrid （1-2, 2-1, 7-1, 13-3） developed by Primorsky Scientific Research Institute of Agriculture. Five variants of N6 medium （N6-I, N6-2, N6-3, Mix-l, N6-4） were used as basal medium. Mean value of callus induction frequency on three types of conditions ranged from 5.68% to 9.44% and the difference was non-significantly. In general, callus derived from donor plants grown on condition of open space ＋ growth chambers showed significantly better performances for plant regeneration （0.23 green regenerants on anther and 3.77 green regenerants on callus） and productive shoot regeneration （0.06 productive regenerants on anther and 0.56 productive regenerants on callus）. Favourable conditions for donor plant growth in open space positively affect on callus induction and regenaration. It is possible to get assured results on many hybrids, but not the highest. In growth chamber, frequency of callus induction can be the maximal only on some samples, few hybrids are resulted in deficiency of callus induction.展开更多
文摘以往的研究证明由左心房逆行灌注冲洗肺,在短期肺保存中获得良好效果。但此法是否适用于长时间的肺保存,至今尚无资料。也有资料表明:由于肺静脉端较低的血管阻力,常规用于顺行肺灌注—肺保存中的血管扩张素一前列腺素E_1可能不必用于逆行灌注—肺保存。本研究旨在估价逆行灌注和前列腺素E_1(PGE_1)在24h肺保存中的作用。实验狗分为两组,University of Wisconsin保存液(UW液)作为冲洗和保存液。在A组(n=7),250μg PGE_1在进行灌注之前注入供体的肺动脉,另外250μg混合在UW液中。B组为对照组,不用PGE_1。在4℃下保存22～25h后,作同种异体左肺移植。在肺移植之前和之后的10—,30—,60—,120—min测试血流动力学指标、血气分析、呼吸道阻力和肺顺应性。结果表明,经120 min的在体再灌注后,氧和二氧化碳分压(kPa)分别为85.52±3.20和4.93±0.40(A组);79.80±3.87和4.93±0.40(B组)。血流动力学指标(kPa,A组对B组):肺动脉压2.67±0.13对3.73±0.27(P<0.05);右房压0.53±0.13对1.07±0.13(P<0.05);肺血管阻力(dyne·s·cm^(-5))1109±51对1525±133(P<0.05)。气道阻力(kPa/L·s)2.16±0.10对2.35±0.10(P=NS);肺顺应性(CC╱cm)30±1对26±1(P<0.05)。在B组,经2h的再灌注后,气道阻力显著高于实验前的基值(19±1)(P<0.05)。电子显微镜观察表明:除组织间隙水肿外,Ⅰ型肺上皮细胞、毛细血管内皮和微细支气管上皮均现正常。Ⅱ型肺上皮细胞的板层小体内容物减少或消失。上述改变在两组间无明显差别。结论:用UW液逆灌保存犬肺24h是可行的。PGE_1有助于减小肺血管阻力、气道阻力和改善肺顺应性。
文摘An experiment was conducted to find the effect of three types of donor plants growing conditions （growth chamber, open space ＋ growth chambers and open space） on callus induction and subsequent plant regeneration and productive shoot regeneration from the anthers culture in vitro of four rice hybrid （1-2, 2-1, 7-1, 13-3） developed by Primorsky Scientific Research Institute of Agriculture. Five variants of N6 medium （N6-I, N6-2, N6-3, Mix-l, N6-4） were used as basal medium. Mean value of callus induction frequency on three types of conditions ranged from 5.68% to 9.44% and the difference was non-significantly. In general, callus derived from donor plants grown on condition of open space ＋ growth chambers showed significantly better performances for plant regeneration （0.23 green regenerants on anther and 3.77 green regenerants on callus） and productive shoot regeneration （0.06 productive regenerants on anther and 0.56 productive regenerants on callus）. Favourable conditions for donor plant growth in open space positively affect on callus induction and regenaration. It is possible to get assured results on many hybrids, but not the highest. In growth chamber, frequency of callus induction can be the maximal only on some samples, few hybrids are resulted in deficiency of callus induction.
