以聚乙二醇-二硬脂酰基磷脂酰乙醇胺(PEG-DSPE)为表面活性剂,以维生素E为油相,研制长循环紫杉醇微乳(paclitaxel carried by long circulating microemulsions,TXL-M),并评价其抗肿瘤作用。采用电子显微镜、激光粒度/zeta电位分析仪,观...以聚乙二醇-二硬脂酰基磷脂酰乙醇胺(PEG-DSPE)为表面活性剂,以维生素E为油相,研制长循环紫杉醇微乳(paclitaxel carried by long circulating microemulsions,TXL-M),并评价其抗肿瘤作用。采用电子显微镜、激光粒度/zeta电位分析仪,观察了TXL-M的形态,测定了TXL-M的粒度分布和zeta电位。用昆明种小鼠评价了TXL-M的毒性。采用小剂量化疗方案和动物肿瘤模型,评价了TXL-M的抗肿瘤作用。通过测定给药后不同时间的血浆药物浓度和肿瘤中的药物含量,评价了TXL-M的长循环效果及其在肿瘤中的分布。结果表明,TXL-M呈圆球形,粒径为(98.6±11.2)nm,zeta电位为(?32.4±6.8)mV。采用小剂量给药方案,静脉注射后,与紫杉醇相比长循环微乳显著延长了紫杉醇的血液循环时间,显著提高了紫杉醇的抗肿瘤作用,同时显著减轻了紫杉醇的毒性。总之,TXL-M比紫杉醇更适用于肿瘤的小剂量化疗,有可能成为一种较好的抗肿瘤药物。展开更多
目的:制备阿克拉霉素A的长循环微乳(aclacinomycin A long circulation micoremulsions,ALM)用于肿瘤的小剂量化疗。方法:以聚乙二醇-二硬脂酰基磷脂酰乙醇胺为表面活性剂,制备了ALM,并测定了其理化性质。通过比较ALM与阿克拉霉素A(ACA...目的:制备阿克拉霉素A的长循环微乳(aclacinomycin A long circulation micoremulsions,ALM)用于肿瘤的小剂量化疗。方法:以聚乙二醇-二硬脂酰基磷脂酰乙醇胺为表面活性剂,制备了ALM,并测定了其理化性质。通过比较ALM与阿克拉霉素A(ACA)的血药浓度和急性毒性,评价了ALM的长循环及其降低ACA毒性的作用。采用小剂量化疗方案给药,评价了ALM的抗肿瘤作用。结果:ALM的粒径为(118.4±8.2)nm,Zeta电位为(-32.6±7.2)mV,载药率为(98.2±1.6)%(n=3)。与ACA相比,ALM的血药浓度显著提高,急性毒性较低,抗肿瘤作用较强,且更适用于肿瘤的小剂量化疗(P<0.01)。结论:ALM有可能成为一种适用于肿瘤小剂量化疗的药物。展开更多
Mutagenesis of Lactobacillus casei subsp, rhamnosus Xl-12 after low power microwave irradiation was investigated. Under a microwave power of 400 W and irradiation length of 3 min, a mutated strain W4-3-9 with high-yie...Mutagenesis of Lactobacillus casei subsp, rhamnosus Xl-12 after low power microwave irradiation was investigated. Under a microwave power of 400 W and irradiation length of 3 min, a mutated strain W4-3-9 with high-yield L-lactic acid was obtained by screening. Compared with the starting strain X1-12, the L-lactic acid production of W4-3-9 was increased by 58.0% at a concentration of 115.8 g/L. The strain maintained the capability of producing a high L-lactic acid level after 10 generations. Cell surface morphology and DNA structures of parental and mutated strains were observed by atomic force microscopy （ AFM ）. Amplified fragment length polymorphism （AFLP） analysis suggested the difference in AFLP band pattern between the mutated and non-mutated strains. Sequencing and BLAST analysis revealed that the catalytic site of lactate dehydrogenase （DHL） was changed due to the microwave induced mutation.展开更多
文摘以聚乙二醇-二硬脂酰基磷脂酰乙醇胺(PEG-DSPE)为表面活性剂,以维生素E为油相,研制长循环紫杉醇微乳(paclitaxel carried by long circulating microemulsions,TXL-M),并评价其抗肿瘤作用。采用电子显微镜、激光粒度/zeta电位分析仪,观察了TXL-M的形态,测定了TXL-M的粒度分布和zeta电位。用昆明种小鼠评价了TXL-M的毒性。采用小剂量化疗方案和动物肿瘤模型,评价了TXL-M的抗肿瘤作用。通过测定给药后不同时间的血浆药物浓度和肿瘤中的药物含量,评价了TXL-M的长循环效果及其在肿瘤中的分布。结果表明,TXL-M呈圆球形,粒径为(98.6±11.2)nm,zeta电位为(?32.4±6.8)mV。采用小剂量给药方案,静脉注射后,与紫杉醇相比长循环微乳显著延长了紫杉醇的血液循环时间,显著提高了紫杉醇的抗肿瘤作用,同时显著减轻了紫杉醇的毒性。总之,TXL-M比紫杉醇更适用于肿瘤的小剂量化疗,有可能成为一种较好的抗肿瘤药物。
文摘目的:制备阿克拉霉素A的长循环微乳(aclacinomycin A long circulation micoremulsions,ALM)用于肿瘤的小剂量化疗。方法:以聚乙二醇-二硬脂酰基磷脂酰乙醇胺为表面活性剂,制备了ALM,并测定了其理化性质。通过比较ALM与阿克拉霉素A(ACA)的血药浓度和急性毒性,评价了ALM的长循环及其降低ACA毒性的作用。采用小剂量化疗方案给药,评价了ALM的抗肿瘤作用。结果:ALM的粒径为(118.4±8.2)nm,Zeta电位为(-32.6±7.2)mV,载药率为(98.2±1.6)%(n=3)。与ACA相比,ALM的血药浓度显著提高,急性毒性较低,抗肿瘤作用较强,且更适用于肿瘤的小剂量化疗(P<0.01)。结论:ALM有可能成为一种适用于肿瘤小剂量化疗的药物。
基金Sponsored by the National Natural Science Foundation of China(Grant No.50778053)
文摘Mutagenesis of Lactobacillus casei subsp, rhamnosus Xl-12 after low power microwave irradiation was investigated. Under a microwave power of 400 W and irradiation length of 3 min, a mutated strain W4-3-9 with high-yield L-lactic acid was obtained by screening. Compared with the starting strain X1-12, the L-lactic acid production of W4-3-9 was increased by 58.0% at a concentration of 115.8 g/L. The strain maintained the capability of producing a high L-lactic acid level after 10 generations. Cell surface morphology and DNA structures of parental and mutated strains were observed by atomic force microscopy （ AFM ）. Amplified fragment length polymorphism （AFLP） analysis suggested the difference in AFLP band pattern between the mutated and non-mutated strains. Sequencing and BLAST analysis revealed that the catalytic site of lactate dehydrogenase （DHL） was changed due to the microwave induced mutation.
