玉米种子安全贮存新技术──聚乙烯薄膜封垛隔离

玉米种子安全贮存新技术──聚乙烯薄膜封垛隔离玉米种子因其本身的结构特点和生理特性，历来是长期贮存的难题。公主岭市种子公司根据多年实践经验总结出玉米种子过夏安全贮存新技术，即采用聚乙烯薄膜封垛隔离法，此方法是种子垛的表层和垛内袋与袋的间隙处，然后再向纵...

Ecological Assessment on the Introduced Sonneratia caseolaris and S. apetala at the Mangrove Forest of Shenzhen Bay, China

yTwo exotic species, Sonneratia caseolaris (L.) Engl. and S. apetala B. Ham., were introduced to Futian Mangrove Forest Nature Reserve, Shenzhen Bay, in 1993 for afforestation. Winter cold caused frigid harm but did not appear to be an obstacle to the introduction. The cold tolerance of the parental and filial generation increased in several years. The two Sonneratia spp. could propagate by seeds and disperse in Shenzhen Bay. While the two species grew faster than indigenous species, at the same time they promoted the growth of indigenous species, but they could not replace indigenous mangrove species. Suitable habitat was more important than the distance from the source in the natural spreading for the two species. The niche of the two species did not overlap with the niche of indigenous species. The two species did not self regenerate, although they could disperse on localized area in Shenzhen Bay. Therefore, it was unlikely that the two species would pose ecological disaster.

Variability of leaf functional traits of invasive tree Rhus typhina L. in North China

Functional traits, specifically leaf functional traits, are core-topics to explore importance to the invasion success of invasive plant species. This study aims to address the differences in leaf functional traits and their corresponding variability of the invasive tree staghorn sumac Rhus typhina L. with different invasion success, including lower and higher invasion success, in two climatic regions in North China, including a warm temperate region and a cold temperate region. No significant differences were found for leaf functional traits of staghorn sumac across different invasion success. However, the variability of leaf chlorophyll and nitrogen concentrations of staghorn sumac under higher invasion success were approximately 66.023% and 68.615% higher than those under lower invasion success, respectively. The leaf chlorophyll and nitrogen concentrations of staghorn sumac in the warm temperate region were approximately 18.432% and 16.337% higher than those in cold temperate region, respectively. The variability of specific leaf area of staghorn sumac in warm temperate region was approximately 59.802% higher than that in cold temperate region. Accordingly, leaf chlorophyll and N concentrations as well as specific leaf area of staghorn sumac and their corresponding variability may play an essential role in shaping ecological success of studied invader along a climatic gradient.

Biodiversity and Invasibility: Distribution Patterns of Invasive Plant Species in the Himalayas, Nepal

Invasive plant species are exerting a serious threat to biological diversity in many regions of the world. To understand plant invasions this study aims to test which of the two plant invasiveness hypotheses; ‘low native diversity' vs. ‘high native diversity', is supported by the regional distribution patterns of invasive plant species in the Himalayas,Nepal. This study is based on data retrieved from published literatures and herbarium specimens. The relationship between invasive plant species distribution patterns and that of native plant species is elucidated by scatter plots, as well as by generalized linear models. The native plant species and invasive plant species have similar distribution patterns and the maximum number of invasive plant species is found in the same altitudinal range where the highest richness for native tree species is found. There is a clear trend of higher invasive plant richness in regions where native tree species richness is relatively high.Consequently, the native plant richness is highest in the central phytogeographic region, followed by the eastern and the western regions, respectively. The invasive plant species also follows a similar trend.Additionally, the invasive plant species richness was positively correlated with anthropogenic factors such as human population density and the number of visiting tourists. This study supports the hypothesis that ‘high native diversity' supports or facilitates invasive plant species. Further, it indicates that nativeand invasive plant species may require similar natural conditions, but that the invasive plant species seem more dependent and influenced by anthropogenic disturbance factors.

Evaluation of Extracts of Native and Non-Native Plant Species for the Suppression of Cogongrass （Imperata cylindrica L.）

Effects of the extracts of muhly grass （Muhlenbergia capillaries Lam.）, chenopodium （Chenopodium ambrosioides L.） and black walnut （Juglans nigra L.） on the growth of cogongrass （Imperata cylindrica L.） were studied. None purified extracts from these plant species were used to evaluate survival, shoot and root growth of cogongrass. Results of plant species extracts on the production of roots, shoot and rhizomes of cogongrass genets and ramets were determined on a weekly basis. The extracts significantly reduced the growth and survival of cogongrass when compared to the control. Results indicated that for the in vivo studies, chenopodium extracts, black walnut and muhly grass extracts reduced growth, root and shoot biomass of cogongrass by 50 %, 30 % and 41% respectively, compared to control at 8 weeks after planting. These studies indicated plant species with potential for the biological control of cogongrass, an invasive species in the Gulf Coast States.

Zoospore-derived monoecious gametophytes in Undaria pinnatifida(Phaeophyceae)

The annual life cycle of the brown seaweed Undariapinnatifida （Harvey） Suringer comprises a macroscopic diploid sporophyte stage and a microscopic haploid gametophyte stage. In 2011, an unusual zoospore-derived monoecious gametophyte isolate （designated as line 10-5-3） of U. pinnatifida was observed. To understand this phenomenon, a comprehensive screening of eighty-two previously identified male gametophyte cultures, isolated from three randomly selected cultivars （lines 10, 7, and 5） was performed. Thirty-six of the isolates developed both antheridia and oogonia on the same filamentous fragment in a standard gametogenesis test （SGT： 18℃, 60 umol photons/（m2.s））. Selfing of the monoecious gametophyte or crossing it with a normal male gametophyte both gave rise to morphologically normal sporophytic offspring. However, crossing resulted in a much higher fertilization rate （89.7%）. The hybrid and selfed sporophytic offspring were grown to maturity in flow tanks at an ambient temperature of 10-18℃ over a period of 69 days. Active zoospores were released from both types of mature sporophylls. The majority of these developed into male gametophytes, while 15%-20% developed into the observed monoecious structures on the same filament. Using PCR amplification it was found that all the monoecious gametophyte isolates and the sporophytic offspring resulting from the selfing and crossing lacked the femalelinked microsatellite sequence （a part of the locus Up-AC-2A8, GenBank accession No. AY738602.1）, indicating their male nature. U.pinnatifida is an invasive species in some regions and the implications of the above findings for this species in nature are briefly discussed.

Naturalization of Landscaping Woody Plant, Magnolia obovata Potentially Invasive Species

Magnolia obovata, a tree species native to Japan, is a traditional landscaping tree that has also been introduced to various countries. M. obovata individuals have recently emerged in non-planting areas in Korea, prompting us to investigate its distribution by establishing Dosolsan in an urban forest of Daejeon City as the target site. In order to determine its naturalization, the study explored the status of population growth by examining the diameter at breast height of the individuals, and diameter diminution quotient was calculated. Cores of the trees were collected, and the age distribution was estimated by regression analysis. Reproduction possibility was analyzed by verifying the flowerblooming and fruit-bearing. Spontaneous colonization was investigated in the other potential sites which have different location and environment, respectively.The diameter distribution showed a reverse J-shaped curve, and the diminution quotient was ≥ 1.8. The population was composed of different generations,thus confirming the growth of the population. The distributed M. obovata grew and flowered normally,and followed a normal life cycle. The same phenomenon was observed in other planting areas,residual forests in urban or rural areas, alluvial islands, and mountain forests. The continuous spread of M. obovata in new ecosystems may be attributable to its long-term distribution by birds, relatively fast growth, and maturity time. Investigations on the naturalization and spread of M. obovata in Korea, as well as in various countries, are warranted.

Red Palm Weevil, Rhynchophorus ferrugineus （Olivier）： Economic Importance, Biology, Biogeography and Integrated Pest Management

The Red Palm Weevil （RPW）, Rhynchophorus ferrugineus （Olivier）, （Coleoptera, Curculionidae, Rhynchophorinae）, is an invasive species that is originated from Southeast Asia. It has.invaded Middle East and several countries of the Mediterranean Basin during the last three decades where it attacks palm trees. During the last three decades, multiple introductions of RPW to the Middle East, Europe and Caribbean （Island of Curacao, Netherland Antilles）, Lebanon and United States of America （Laguna Beach, Orange County, California） （USA） have occurred and the RPW is now a serious pest of many palm species. Duration of all life parameters varies significantly where the entire life cycle takes about 45 to 298 days. Different artificial diets were developed and were tested for mass rearing of RPW. Control of RPW is difficult due to the concealed nature of the life cycle of the pest. Integrated Pest Management （IPM） strategy has been applied successfully to deal with RPW problem. The IPM strategy consists of various preventive and curative methods; those methods were categorized in nine categories in this review. The main objective of this work was to review the existing knowledge on RPW＇s different aspects, with an ultimate aim of revealing the actual situation of the research on RPW.

Preliminary Study on Geographical Variation of Cytochrome b gene and ITS2-rDNA among Populations of Rhynchophorus ferrugineus

The Red Palm Weevil （RPW）, Rhynchophorus ferrugineus （Olivier）, （Coleoptera, Curculionidae, Rhynchophorinae）, is an invasive species that is originated from Southeast Asia. It has invaded Middle East, several countries of the Mediterranean Basin, Caribbean （Island of Curacao, Netherland Antilles）, Lebanon and United States of America （Laguna Beach, Orange County, California） during the last three decades where it attacks palm trees. This study investigated the geographical variation among geographic populations of RPW collected from twelve invaded countries using cytochrome b （Cytb） and ITS2 partial sequences. The comparison among the Cytb sequences resulted in three different haplotypes designated as HB 1 to HB3. The three haplotypes were subdivided into two phylogenetic groups according to their geographic positions： （1） the ＂Multi-Continent＂ group containing HB 1 haplotype detected in eight populations belonging to three different Continents such as Africa-Egypt, Asia-Kingdom of Saudi Arabia and Turkey and Europe-Spain, Italy, Greece, Cyprus and France, and （2） the ＂Asian＂ group includes four populations from Iran （harboring HB2 haplotype）, Pakistan, UAE, and Oman （harboring HB3 haplotype）. This mitochondrial pattern of genetic diversity suggests that the tested RPW populations subdivided genetically into different sub-populations under the influence of genetic drift. According to these results we concluded that the twelve studied RPW populations are originating from different source populations in the RPW area of origin.

Micronutrient input into a mangrove ecosystem in Jobos Bay,Puerto Rico,by the exotic green iguana Iguana iguana

Biological invasions are an important and growing component of global environmental change （Vitousek et al., 1996）. Hundreds of billions of dollars are lost each year to invasive species damage and management （Pimentel et al., 2001）. Scientists have responded by conducting research to understand the biology of the invasive species itself, in the hope that such information will allow effective control, and examining the impact of the invader on native taxa to determine the nature and magnitude of its effect.

Mutagenic Effect of Three Invasive Species through Allium Cepa Bioassay

Invasive alien species are a global threat to biodiversity that affects protected areas around the world. The occupation of new environments by these plants is a problem to be solved and it is essential to investigate all the aspects that allow this successful to find solutions to this question, such as its mutagenic effects. Thus, this study aimed to evaluate the mutagenic effect of leaves extracts of Acacia mangium Willd, Artocarpus heterophyllus Lam and Eriobothrya japonica （Thunb.） Lindl through Allium cepa bioassay. For this, A. cepa seeds were submitted to continuous and discontinuous （acute and chronic） treatments in medium with water （negative control） or four concentration of each extract （1, 5, 10 and 50 mg/mL）. The mitotic index was affected at all concentrations of three extracts tested in all treatments, continuous and discontinuous. Aneugenic effects were not related to any treatment tested. E. japonica extract induced clastogenic effects at 1, 5 and 10 mg/mL in continuous treatment, 5 and 10 mg/mL in acute discontinuous treatment and at 10 mg/mL in chronic discontinuous treatment. Clastogenic effect was also observed at 10 mg/mL ofA. heterophyllus extract in continuous and acute discontinuous treatments.

Tests to evaluate the ecological impact of treated ballast water on three Chinese marine species

Ballast water has been a topic of concern for some time because of its potential to introduce invasive species to new habitats. To comply with the International Convention for the Control and Management of Ships' Ballast Water and Sediments, members of the International Maritime Organization(IMO) must equip their ships with on-board treatment systems to eliminate organism release with ballast water. There are many challenges associated with the implementation of this IMO guideline, one of which is the selection of species for testing the ecological impacts of the treated ballast water. In the United States, ballast water toxicity test methods have been defined by the United States Environmental Protection Agency. However, the test methods had not been finalized in China until the toxicity test methods for ballast water were established in 2008. The Chinese methods have been based on species from three trophic levels: Skeletonema costatum, Neomysis awatschensis, and Ctenogobius gymnauchen. All three species live in broad estuarine and open sea areas of China; they are sensitive to reference toxicants and acclimatize easily to different conditions. In this paper, the biological characteristics, test processes and statistical analysis methods are presented for the three species. Results indicate that the methods for evaluating these three organisms can be included in the ecological toxicity tests for treated ballast water in China.

Luminescence Properties of Nanometer Si with Embedded Structure

Blue luminescence at about 431nm is obtained from epitaxial silicon after C+ implantation,annealing in hydrogen ambience and chemical etching sequentially.With increase of chemical etching,the blue peak is enhanced at first,then decreased and substituted by a red peak at last.C=O compounds are introduced during C+ implantation and embedded in the surface of nanometer Si formed during annealing,and at last is formed nanometer silicon with embedded structure,which contributes to the blue emission.Presented is the possible mechanism of photoluminescence.

Invasion and Morphological Variation of the Non- Indigenous Barnacle Chthamalus challengeri(Hoek, 1883) in Yangshan Port and its Surrounding Areas

Invasive species generally possess unique characteristics that allow them to survive the invasion process in order to es- tablish and spread in new habitats. Successful invaders must resist both physical and physiological stresses associated with the changing environment. A common littoral barnacle, Chthamalus challengeri Hoek, 1883 （Crustacea, Cirripedia）, which is native to Japan, South Korea and northern China, has become established in the high-littoral zone adjacent to Yangshan Port, Shanghai, China. A comparison of the morphology of Chthamlus species from Zhoushan archipelago with previous description indicates the occur- rence of C. challengeri. The new immigrant becomes a dominant species in certain high-intertidal habitats of the adjacent area to of Yangshan Port. C. challengeri was found in part of sampling sites in Zhoushan in 2010; however, it dispersed to all the eleven sam- piing sites in 2012. Densities of C.challengeri had increased over 10 times in the last 2 years, with the highest mean value reaching 39533 ± 6243 ind. m-2 in the new habitat. The specific ratios of both operculum area （Sa） to base area （SA） and average height of pa- rietal plates （H） to length of base （L） revealed that C. challengeri displays morphological changes to resist stronger currents in the new habitats for invasion.

Uses of Invasive Alien Plant Species in Rema-Kalenga Wildlife Sanctuary of Bangladesh

An exploratory survey was conducted on the uses and role of invasive alien species(IAS) to local livelihoods in the Rema-Kalenga Wildlife Sanctuary in the northeastern hilly region of Bangladesh.Vegetation was sampled in 50 quadrates,each 10 m×10 m,and 25 line transects,each 50 m×20 m,through a combination of random and systematic sampling.Group discussions were also arranged with fifty local inhabitants(17 % of the total inhabitants) to learn the various uses of IAS.A total of 17 alien plant species belonging to 10 different families,of which Leguminosae constitutes the highest number(5 species),include tree(53%) followed by herb(12%),shrub(12%) and others(weed,climber and palm).The majority of the identified species were found to be used for fuel,followed by timber production,medicinal or curative uses,fodder,and many others.In addition to providing various forest products,alien tree species planted in forest plantation help provide many vital ecosystem services such as soil amelioration through nitrogen fixation.It is true that the alien species have some negative impacts on local ecosystems,and not all of them are harmful.Therefore,a national programme must be initiated to distinguish the harmful from the harmless species and to identify the uses and impacts of the former and latter.

Impact of Sphagneticola trilobata on Plant Diversity in Soils in South-East Viti Levu, Fiji

Wedelia （Sphagneticola trilobata （L.） Pruski） has become one of the most dominant invasive plant species in Fiji. However, the soil seed bank of its monospecific stand and its ability to reproduce by seed is relatively unknown. A soil seed bank study was undertaken in a monospecific stand ofS. trilobata in Sawani, Natavea and Wainivesi in south-east Viti Levu, Fiji in March 2012. The soil samples were collected from within 1.0 m2 quadrat taken at 10 spots in each of the study areas and spread thinly over a base of Yates Thrive Premium potting mix in seedling trays and placed in a glasshouse at Koronivia Research Station, Fiji. A total of 23, 26 and 33 plant species were found in the soil seed bank in Wainivesi, Sawani and Natavea respectively which may have succumbed to S. trilobata invasiability. There were ca. 3,800 （17%）, 2,100 （11%） and 2,600 （6%） germinable S. trilobata seeds-m-2 in the soil seed bank in Wainivesi, Sawani and Natavea areas respectively. This study has demonstrated that S. trilobata seeds may have a role in the spread of the invasive species in Fiji and movement of soil to S. trilobata free areas should be restricted.

Ballast Water Treatment Methods of International Vessels Docking in Loboc Port, Iloilo City, Philippines

This study delved on the ballast water management methods among international vessels docking at Loboc Port, Iloilo City, Philippines and other factors that are considered during ballast operation. The respondents of this study were the seafarers from the 15 international vessels docking at Loboc Port. The findings of the study revealed that the international vessels docking at Loboc Port used the sequential method, flow-through method, chlorination method, hydrogen peroxide, UV （ultraviolet） irradiation and filtration method in ballasting. In order to prevent transfer of alien invasive species, government authorities such as MARINA （Maritime Industry Authority） and Philippine Coast Guard should strictly monitor and implement the ballast water management methods used by international vessels based on the guidelines set by the IMO （International Maritime Organization）.

Assessment of the Allelopathic Potential of an Invasive Alien Weed Hyptis suaveolens （L,） Poit. on Germination of Oryza sativa L.

Hyptis suaveolens （L.） Poit. is an invasive alien weed commonly known as ＂Bana tulst＂ in Odisha belonging to the family Lamiaceae and widely distributed in different parts of India. The leaves of the plant have been reported to be used as stimulant, carminative, sudorific and also as a cure for parasitic cutaneous diseases besides the crude leaf extract is reported to be used against colic and stomach-ache. The leaves are the source of different alkaloids, terpenes and volatile oils having allelopathic effect. In view of this the present work was carried out with an aim to investigate the allelopathic effect upon germination of rice （Oryza sativa L.）. The result indicated that the higher concentration of the leaf leachate could decrease the percentage of germination besides identified as a potential bio-herbicide.

Distribution of Invasive Plant Species in Relation to Environmental and Anthropogenic Factors in Five Nature Reserves in Northern China

The relationships between invasive plant species and environmental and anthropogenic factors were analyzed in five nature reserves in northern China. The distribution of invasive plants was analyzed using a Canonical Correspondence Analysis. Stepwise backward multiple regression was then done to identify the most important environmental and anthropogenic factors determining the richness and distribution of alien plants in the reserves. Total invasive plant richness varied was 25 species, 12 species, 13 species, five species and five species across the nature reserves. Invasive species richness decreased in response to environmental factors such as maximum temperature and minimum precipitation and increased in line with human activities, the total area of farmland and volume of freight. Tropical plant species declined from 31% to zero while the species originally from North America gradually increased. In respect of family composition of invasive species, most species belonged to the composites, whose proportion increased from 15%-40% from north to south across the five nature reserves while species from other families varied greatly along this gradient. Life-forms of invasive plant species varied between the reserves, but annual and perennial herbs were generally dominant. Pathways of such species were highly related to human activities and utilizations.