为明确番茄接种根结线虫后基因种类和表达量在转录水平的变化规律,利用RNA-seq对未接种及接种南方根结线虫2龄幼虫6、12、24和48 h的番茄进行转录组测序,探究番茄响应南方根结线虫侵染相关的关键转录因子,并采用qRT-PCR方法对测序结果...为明确番茄接种根结线虫后基因种类和表达量在转录水平的变化规律,利用RNA-seq对未接种及接种南方根结线虫2龄幼虫6、12、24和48 h的番茄进行转录组测序,探究番茄响应南方根结线虫侵染相关的关键转录因子,并采用qRT-PCR方法对测序结果进行验证。结果显示,接种南方根结线虫后6、12、24和48 h分别有350、390、580、1154个基因差异表达,其中差异表达转录因子分别为11、11、19、50个。这些转录因子属于15个家族,其中数量最多的为MYB家族和bHLH家族(均为20个),其次是ERF家族19个、WRKY家族15个、bZIP家族9个。南方根结线虫侵染过程差异表达最明显的主要为ERF、WRKY、MYB和bHLH家族转录因子,其中Solyc03g005520、Solyc02g094270和Solyc09g066350显著上调,接种后48 h log2FC分别为9.16、6.49和6.33;Solyc02g079280、Solyc12g100140和Solyc04g072460显著下调,接种后48 h log2FC分别为-2.60、-1.72和-1.70。qRT-PCR验证结果显示,6个随机选取基因的表达趋势与测序结果基本一致。以上结果表明,ERF、WRKY、MYB和bHLH家族转录因子可能参与番茄与南方根结线虫互作,在番茄响应南方根结线虫侵染反应中发挥着重要的调控作用。展开更多
[ Objective] The study was aimed to determine the biological characteristics of Rhabditis （Oscheius） sp. isolated from a rhizosphere soil of alfalfa field in Hailar City, Hulunbuir League, Inner Mongolia Autonomous ...[ Objective] The study was aimed to determine the biological characteristics of Rhabditis （Oscheius） sp. isolated from a rhizosphere soil of alfalfa field in Hailar City, Hulunbuir League, Inner Mongolia Autonomous Region. [ Method] The last instar larvae of Galleria mellonella and Tenebrio molitor were used as insect hosts. The infectivity of nematode was determined, and its heat tolerance ability was measured by using water bath method. [Result] The infectivity of the nematodes was lower at the dosages of 10, 20 and 40 IJs/larva, but higher at the dosages of 80,160, 32.0 and 640 IJs/larva. The corrected mortality （CM） of G. mellonella at 96 h and T. molitor at 120 h were both above 90%. The nematode infectivity was highest at the dosage of 80 IJs/larva when the hosts＇ CM reached 100% after 120 h. About 25% nematode individuals could survive in 36℃ water bath for 2 d in the densities of 1 000 -5 000 IJ/ml, after 6 d, there were still 3% -9% individuals survived, until 9 d the nematodes died totally. A hundred percent of the nematode individuals died in 38℃ and 40 ~C water bath after 6 h and 2 h, respectively. [Conclusion] Rhabditis （ O. ） sp. has a higher potential to cause the host mortality and represents heat tolerance up to 36℃.展开更多
文摘为明确番茄接种根结线虫后基因种类和表达量在转录水平的变化规律,利用RNA-seq对未接种及接种南方根结线虫2龄幼虫6、12、24和48 h的番茄进行转录组测序,探究番茄响应南方根结线虫侵染相关的关键转录因子,并采用qRT-PCR方法对测序结果进行验证。结果显示,接种南方根结线虫后6、12、24和48 h分别有350、390、580、1154个基因差异表达,其中差异表达转录因子分别为11、11、19、50个。这些转录因子属于15个家族,其中数量最多的为MYB家族和bHLH家族(均为20个),其次是ERF家族19个、WRKY家族15个、bZIP家族9个。南方根结线虫侵染过程差异表达最明显的主要为ERF、WRKY、MYB和bHLH家族转录因子,其中Solyc03g005520、Solyc02g094270和Solyc09g066350显著上调,接种后48 h log2FC分别为9.16、6.49和6.33;Solyc02g079280、Solyc12g100140和Solyc04g072460显著下调,接种后48 h log2FC分别为-2.60、-1.72和-1.70。qRT-PCR验证结果显示,6个随机选取基因的表达趋势与测序结果基本一致。以上结果表明,ERF、WRKY、MYB和bHLH家族转录因子可能参与番茄与南方根结线虫互作,在番茄响应南方根结线虫侵染反应中发挥着重要的调控作用。
基金Supported by Ministry of Science and Technology of China on 863 Program"Soil Technique of Nematode Repair Biological Pollution"(2006AA06Z354)Support Plan Program"Production Technology ofHigh Efficiency Standard on Main Fruit Trees"(2008BAD92B08)BeijingGovernment on Purchasing Technology Service Program(2007DAg03B03-07)~~
文摘[ Objective] The study was aimed to determine the biological characteristics of Rhabditis （Oscheius） sp. isolated from a rhizosphere soil of alfalfa field in Hailar City, Hulunbuir League, Inner Mongolia Autonomous Region. [ Method] The last instar larvae of Galleria mellonella and Tenebrio molitor were used as insect hosts. The infectivity of nematode was determined, and its heat tolerance ability was measured by using water bath method. [Result] The infectivity of the nematodes was lower at the dosages of 10, 20 and 40 IJs/larva, but higher at the dosages of 80,160, 32.0 and 640 IJs/larva. The corrected mortality （CM） of G. mellonella at 96 h and T. molitor at 120 h were both above 90%. The nematode infectivity was highest at the dosage of 80 IJs/larva when the hosts＇ CM reached 100% after 120 h. About 25% nematode individuals could survive in 36℃ water bath for 2 d in the densities of 1 000 -5 000 IJ/ml, after 6 d, there were still 3% -9% individuals survived, until 9 d the nematodes died totally. A hundred percent of the nematode individuals died in 38℃ and 40 ~C water bath after 6 h and 2 h, respectively. [Conclusion] Rhabditis （ O. ） sp. has a higher potential to cause the host mortality and represents heat tolerance up to 36℃.
