Objective MIF, a potent pro-inflammatory cytokine, plays a pivatol role in the pathogenesis of sepsis. A novel CATT microsatellite at position -794 of the human MIF gene functionally affects the activity of the MIF pr...Objective MIF, a potent pro-inflammatory cytokine, plays a pivatol role in the pathogenesis of sepsis. A novel CATT microsatellite at position -794 of the human MIF gene functionally affects the activity of the MIF promoter and correlates with the disease severity in rheumatoid arthritis, ulcerative colitis, atopy or sarcoidosis. The purpose of this study is to determine whether MIF microsatellite is associated with the susceptibility to and outcome of severe sepsis. Methods After approval by the hospital and national ethics committee, written informed consent was obtained from the patient or a first degree relative. Blood samples were obtained from 98 postoperative patients with severe sepsis (sepsis group) and 73 controls. Microsatellite was determined using polymerase chain reaction (PCR) with a FAM-labeled upstream primer combining capillary electrophoresis. Statistical analysis was done by Fisher’s exact test. P < 0.05 was regarded as statistically significant. Results The underlying diseases for the severe sepsis patients were peritonitis, pancreatitis, pneumonia and multiple trauma. The APACH II score in sepsis was 22.5±2.1, the mortality in sepsis patients reached 51%. There is no significant difference in the race, age, sexual. Four kinds of alleles with 4, 5, 6, or 7-CATT repeat units and seven kinds of genotypes with 4/4、4/5、4/6、4/7、5/5、5/6、6/6 CATT repeat units were detected among these patients either with or without sepsis. The genotype distribution and allele frequencies between sepsis individuals and controls, between survivors and non-survivors didn’t differ (P > 0.05). Conclusion This study shows that the functional CATTn microsatellite in the promoter of macrophage migration inhibitory factor is neither related to the incidence of severe sepsis nor to the fatal outcome of sepsis.展开更多
目的研究霉酚酸酯对糖尿病大鼠的保护作用,并探讨其作用机制,对MCP-1、MIF表达的影响。方法选取雄性Wistar大鼠55只,行右肾切除术后两周,随机分成模型组和正常对照组(A组),将糖尿病模型组大鼠随机分为糖尿病大鼠对照组(B组)和糖尿病大...目的研究霉酚酸酯对糖尿病大鼠的保护作用,并探讨其作用机制,对MCP-1、MIF表达的影响。方法选取雄性Wistar大鼠55只,行右肾切除术后两周,随机分成模型组和正常对照组(A组),将糖尿病模型组大鼠随机分为糖尿病大鼠对照组(B组)和糖尿病大鼠治疗组(C组)。应用酶免疫分析法测定大鼠24h尿白蛋白排泄率,采用HE、PAS染色,行肾脏组织形态学分析,用Real time FQ-PCR检测MCP-1、MIF等细胞因子的mRNA表达情况。结果和B组(13.71±0.62)相比,C组大鼠尿白蛋白排泄率明显下降(4.32±0.30),差异具有统计学意义(P<0.05);C组KW/BW、MGV、FMA比B组有不同程度的下降(P<0.05);C组大鼠肾脏MCP-1、MIF的mRNA表达有所下调(P<0.05),但仍高于A组;MCP-1、MIFmRNA表达水平与UAER成正相关(r=0.703,P<0.05;r=0.657,P<0.05);MCP-1mRNA表达水平与KW/BW、MGV、FMA成正相关(r=0.651,P<0.05;r=0.639,P<0.05;r=0.771,P<0.05);MIFmRNA表达水平与KW/BW、MGV、FMA成正相关(r=0.567,P<0.05;r=0.593,P<0.05;r=0.876,P<0.05)。结论霉酚酸酯对糖尿病大鼠肾脏可起到保护作用,其机制可能与下调MCP1、MIFmRNA的表达有关。展开更多
文摘Objective MIF, a potent pro-inflammatory cytokine, plays a pivatol role in the pathogenesis of sepsis. A novel CATT microsatellite at position -794 of the human MIF gene functionally affects the activity of the MIF promoter and correlates with the disease severity in rheumatoid arthritis, ulcerative colitis, atopy or sarcoidosis. The purpose of this study is to determine whether MIF microsatellite is associated with the susceptibility to and outcome of severe sepsis. Methods After approval by the hospital and national ethics committee, written informed consent was obtained from the patient or a first degree relative. Blood samples were obtained from 98 postoperative patients with severe sepsis (sepsis group) and 73 controls. Microsatellite was determined using polymerase chain reaction (PCR) with a FAM-labeled upstream primer combining capillary electrophoresis. Statistical analysis was done by Fisher’s exact test. P < 0.05 was regarded as statistically significant. Results The underlying diseases for the severe sepsis patients were peritonitis, pancreatitis, pneumonia and multiple trauma. The APACH II score in sepsis was 22.5±2.1, the mortality in sepsis patients reached 51%. There is no significant difference in the race, age, sexual. Four kinds of alleles with 4, 5, 6, or 7-CATT repeat units and seven kinds of genotypes with 4/4、4/5、4/6、4/7、5/5、5/6、6/6 CATT repeat units were detected among these patients either with or without sepsis. The genotype distribution and allele frequencies between sepsis individuals and controls, between survivors and non-survivors didn’t differ (P > 0.05). Conclusion This study shows that the functional CATTn microsatellite in the promoter of macrophage migration inhibitory factor is neither related to the incidence of severe sepsis nor to the fatal outcome of sepsis.
文摘目的研究霉酚酸酯对糖尿病大鼠的保护作用,并探讨其作用机制,对MCP-1、MIF表达的影响。方法选取雄性Wistar大鼠55只,行右肾切除术后两周,随机分成模型组和正常对照组(A组),将糖尿病模型组大鼠随机分为糖尿病大鼠对照组(B组)和糖尿病大鼠治疗组(C组)。应用酶免疫分析法测定大鼠24h尿白蛋白排泄率,采用HE、PAS染色,行肾脏组织形态学分析,用Real time FQ-PCR检测MCP-1、MIF等细胞因子的mRNA表达情况。结果和B组(13.71±0.62)相比,C组大鼠尿白蛋白排泄率明显下降(4.32±0.30),差异具有统计学意义(P<0.05);C组KW/BW、MGV、FMA比B组有不同程度的下降(P<0.05);C组大鼠肾脏MCP-1、MIF的mRNA表达有所下调(P<0.05),但仍高于A组;MCP-1、MIFmRNA表达水平与UAER成正相关(r=0.703,P<0.05;r=0.657,P<0.05);MCP-1mRNA表达水平与KW/BW、MGV、FMA成正相关(r=0.651,P<0.05;r=0.639,P<0.05;r=0.771,P<0.05);MIFmRNA表达水平与KW/BW、MGV、FMA成正相关(r=0.567,P<0.05;r=0.593,P<0.05;r=0.876,P<0.05)。结论霉酚酸酯对糖尿病大鼠肾脏可起到保护作用,其机制可能与下调MCP1、MIFmRNA的表达有关。
