With the aging problem increasing seriously, how to make older people live with dignity is no longer a personal problem, a family problem, but gradually becomes a hot topic of the society. When institutional care is d...With the aging problem increasing seriously, how to make older people live with dignity is no longer a personal problem, a family problem, but gradually becomes a hot topic of the society. When institutional care is difficult to meet the pension needs, home care and community care are becoming the choices of the elderly. Based on the way of mutual aid pension, eonarnunity members began to enter our lives. With the ＂time bank＂ setting up and the development in China, China＇s pension system has been further improved. In the late 1990s, some cities in China began to try the pension community service pilot which was a supportive, low cost, high efficiency ＂time bank＂ aged This paper will present the development, the necessity and feasibility of ＂time bank＂, and analyze the problems and find strategies to solve the problems, so as to maintain the sustainable development of＂time bank＂ in our country.展开更多
Objective： The aim of this study was to test the protective effect of mesenchymal stem cells （MSCs） on cardiomyocytes in vitro and to investigate the anti-apoptotic signaling pathway. Methods： MSCs from Sprague-Da...Objective： The aim of this study was to test the protective effect of mesenchymal stem cells （MSCs） on cardiomyocytes in vitro and to investigate the anti-apoptotic signaling pathway. Methods： MSCs from Sprague-Dawley （SD） rats were separated and cultured. MSC medium was collected from MSCs （DMEM） under hypoxia. Cultured cardiomyocytes from neonatal cultured in serum-free Dulbecco＇s modified eagle medium SD rats were exposed to hypoxia/reoxygenation （H/R） and treated with MSC medium. The apoptotic cardiomyocytes were stained with Annexin-V-fluorescein isothiocyanate （FITC）, Hoechst 33342 and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling （TUNEL）. The mitochondrial transmembrane potential of cardiomyocytes was assessed using a fluorescence microscope. The expression of Bcl-2, Bax, cyto- chrome C, apoptosis-induced factor （AIF）, and caspase-3 was tested by Western blot analysis. Results： Our data demonstrated that MSC medium reduced H/R-induced cardiomyocyte apoptosis, increased the Bcl-2/Bax ratio, and reduced the release of cyto- chrome C and AIF from mitochondria into the cytosol. Conclusion： MSCs protected the cardiomyocytes from H/R-induced apoptosis through a mitochondrial pathway in a paracrine manner.展开更多
文摘With the aging problem increasing seriously, how to make older people live with dignity is no longer a personal problem, a family problem, but gradually becomes a hot topic of the society. When institutional care is difficult to meet the pension needs, home care and community care are becoming the choices of the elderly. Based on the way of mutual aid pension, eonarnunity members began to enter our lives. With the ＂time bank＂ setting up and the development in China, China＇s pension system has been further improved. In the late 1990s, some cities in China began to try the pension community service pilot which was a supportive, low cost, high efficiency ＂time bank＂ aged This paper will present the development, the necessity and feasibility of ＂time bank＂, and analyze the problems and find strategies to solve the problems, so as to maintain the sustainable development of＂time bank＂ in our country.
基金supported by the National Natural Science Foundation of China (No.30670868)the Natural Science Foundation of Zheji-ang Province,China (No.R206007)
文摘Objective： The aim of this study was to test the protective effect of mesenchymal stem cells （MSCs） on cardiomyocytes in vitro and to investigate the anti-apoptotic signaling pathway. Methods： MSCs from Sprague-Dawley （SD） rats were separated and cultured. MSC medium was collected from MSCs （DMEM） under hypoxia. Cultured cardiomyocytes from neonatal cultured in serum-free Dulbecco＇s modified eagle medium SD rats were exposed to hypoxia/reoxygenation （H/R） and treated with MSC medium. The apoptotic cardiomyocytes were stained with Annexin-V-fluorescein isothiocyanate （FITC）, Hoechst 33342 and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling （TUNEL）. The mitochondrial transmembrane potential of cardiomyocytes was assessed using a fluorescence microscope. The expression of Bcl-2, Bax, cyto- chrome C, apoptosis-induced factor （AIF）, and caspase-3 was tested by Western blot analysis. Results： Our data demonstrated that MSC medium reduced H/R-induced cardiomyocyte apoptosis, increased the Bcl-2/Bax ratio, and reduced the release of cyto- chrome C and AIF from mitochondria into the cytosol. Conclusion： MSCs protected the cardiomyocytes from H/R-induced apoptosis through a mitochondrial pathway in a paracrine manner.
