The antioxidant activity and its protective effects of Green Tea Extract （GTE） from a Chinese green tea, Hangzhou Longjing, on acute myocardial inthrction （AMI） were investigated in rats. Blood, liver and heart we...The antioxidant activity and its protective effects of Green Tea Extract （GTE） from a Chinese green tea, Hangzhou Longjing, on acute myocardial inthrction （AMI） were investigated in rats. Blood, liver and heart were collected to perform DNA damage test, hepatic antioxidant test, gene expression as well as capillary distribution study. A well-known angiotensin II type I receptor antagonist, Losartan was used as control. It was fbund there were significant reductions in certain DNA damage products＇ levels in GTE and Losartan treated groups compared to saline-treated group. In addition, significant increases in antioxidant enzyme activities and antioxidant gene expressions were observed in GTE and Losartan treated groups. Molphological study also demonstrated the cardioprotective effects of GTE on AMI. In conclusion, GTE has potent and similar cardioprotective effect as Losartan that mav due to its ability to scavenge free radicals.展开更多
Objective: To construct the multi probe ribonuclease protection assay (RPA) template set to be used for detecting expression patterns of MD 2, TLR4, CD14 mRNAs in human peripheral blood mononuclear cells. Methods: The...Objective: To construct the multi probe ribonuclease protection assay (RPA) template set to be used for detecting expression patterns of MD 2, TLR4, CD14 mRNAs in human peripheral blood mononuclear cells. Methods: The designed cDNA fragments of the three genes were generated by polymerase chain reaction (PCR) using specific primers and directionally cloned into EcoR I and Hind III sites of expression plasmid pSP72 containing the T7 promoter, the linearized plasmids was used as template to synthesize anti sense RNA probes. Then we extracted total RNA from peripheral blood mononuclear cells (PBMC) and detected the dynamic expression patterns of the three genes with RPA method. Results: The proper sequence and orientation of the template set were confirmed by sequencing and the template set was successfully used to assay TLR4, MD 2 and CD14 mRNAs in human PBMC. The results showed that the three detected genes decreased transiently 1 3 hours after 100 ng/ml LPS stimulation. Conclusions: These new RPA multi probe set provided valuable tool for the simultaneous quantitative determination of expression of TLR4, CD14 and MD 2 mRNAs in both constitutive and inducible types.展开更多
基金Ministry of Science and Technology of China (Grant No. 2012ZX09103-301-033)the Fundamental Research Funds for the Central Universities (Grant No. 11611206)
文摘The antioxidant activity and its protective effects of Green Tea Extract （GTE） from a Chinese green tea, Hangzhou Longjing, on acute myocardial inthrction （AMI） were investigated in rats. Blood, liver and heart were collected to perform DNA damage test, hepatic antioxidant test, gene expression as well as capillary distribution study. A well-known angiotensin II type I receptor antagonist, Losartan was used as control. It was fbund there were significant reductions in certain DNA damage products＇ levels in GTE and Losartan treated groups compared to saline-treated group. In addition, significant increases in antioxidant enzyme activities and antioxidant gene expressions were observed in GTE and Losartan treated groups. Molphological study also demonstrated the cardioprotective effects of GTE on AMI. In conclusion, GTE has potent and similar cardioprotective effect as Losartan that mav due to its ability to scavenge free radicals.
文摘Objective: To construct the multi probe ribonuclease protection assay (RPA) template set to be used for detecting expression patterns of MD 2, TLR4, CD14 mRNAs in human peripheral blood mononuclear cells. Methods: The designed cDNA fragments of the three genes were generated by polymerase chain reaction (PCR) using specific primers and directionally cloned into EcoR I and Hind III sites of expression plasmid pSP72 containing the T7 promoter, the linearized plasmids was used as template to synthesize anti sense RNA probes. Then we extracted total RNA from peripheral blood mononuclear cells (PBMC) and detected the dynamic expression patterns of the three genes with RPA method. Results: The proper sequence and orientation of the template set were confirmed by sequencing and the template set was successfully used to assay TLR4, MD 2 and CD14 mRNAs in human PBMC. The results showed that the three detected genes decreased transiently 1 3 hours after 100 ng/ml LPS stimulation. Conclusions: These new RPA multi probe set provided valuable tool for the simultaneous quantitative determination of expression of TLR4, CD14 and MD 2 mRNAs in both constitutive and inducible types.
