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贵州湄潭黄杉生态系统保护性评价及保护构想 被引量:3
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作者 胡文彬 《福建林业科技》 2015年第1期173-176,共4页
通过阐述贵州湄潭黄杉(Pseudotsuga sinensis)森林生态型保护区的自然性、多样性、代表性、脆弱性和典型性等自然属性,分析了保护区保护面积的适宜性、生态、经济和社会价值,提出了保护区建设规划构想。
关键词 黄杉 生态系统 保护性评价 构想
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金黄色葡萄球菌α-毒素重组蛋白的免疫原性及其免疫保护性评价 被引量:3
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作者 袁玉兰 郭京蓉 +2 位作者 周继唯 林海涛 张云涛 《中国生物制品学杂志》 CAS CSCD 2020年第9期995-999,共5页
目的评价金黄色葡萄球菌(简称金葡菌)无毒性α-毒素(alpha toxin,AT)重组蛋白的免疫原性及其在金葡菌49521(CP5型)和49525(CP8型)菌株中的免疫保护作用。方法根据NCBI中hla基因参考序列(NC007795.1),人工合成无毒突变hla H35L基因(即mhl... 目的评价金黄色葡萄球菌(简称金葡菌)无毒性α-毒素(alpha toxin,AT)重组蛋白的免疫原性及其在金葡菌49521(CP5型)和49525(CP8型)菌株中的免疫保护作用。方法根据NCBI中hla基因参考序列(NC007795.1),人工合成无毒突变hla H35L基因(即mhla基因)序列,克隆至pET-28a(+)载体中,构建重组质粒pET-28a-mhla,转化至E.coli BL21(DE3)中,IPTG诱导表达,表达产物进行Ni柱亲和层析纯化,SDS-PAGE分析目的蛋白(mAT)的可溶性。用重组蛋白mAT+氢氧化铝佐剂(试验组)和氢氧化铝佐剂(对照组)分别于0、14、28 d经腹部皮下免疫BALB/c小鼠,攻毒前1 d小鼠眼眶采血,检测血清中IgG抗体及其亚型IgG1、IgG2a、IgG2b抗体水平;末次免疫10 d后,分别用致死剂量49521(6.0×108 CFU/只)及49525(3.0×109 CFU/只)菌液经腹腔感染两组小鼠,试验重复3次,统计小鼠存活率。结果经双酶切及测序鉴定,重组质粒pET-28a-mhla构建正确。重组蛋白mAT相对分子质量约为34000,以可溶性形式表达。纯化的重组蛋白mAT可诱导小鼠产生滴度为1∶580000的IgG抗体,且以IgG1亚型(抗体滴度为1∶440000)为主。试验组小鼠对495213次攻毒的保护率分别为75.0%、70.0%和83.3%,对49525菌的保护率分别为80.0%、60.0%和80.0%,均明显高于对照组,差异均有统计学意义(P均<0.05)。结论金葡菌无毒性重组蛋白mAT具有较好的免疫原性和免疫保护作用,可作为多抗原组分疫苗研究的候选抗原。 展开更多
关键词 金黄色葡萄球菌 α-毒素 重组蛋白 免疫原性 保护性评价
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保护性措施效益评价项目浅析
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作者 曾平 焦剑 《水土保持应用技术》 2012年第5期41-43,共3页
多年来,在发展农业生产的同时,为了防治水土流失,保护土地资源、水资源和生物多样性,美国实施了一系列保护性措施,以满足民众对环境质量的要求。为了评估各类措施的环境效益,美国农业部于2004年开始启动保护性措施效益评价项目(CEAP),... 多年来,在发展农业生产的同时,为了防治水土流失,保护土地资源、水资源和生物多样性,美国实施了一系列保护性措施,以满足民众对环境质量的要求。为了评估各类措施的环境效益,美国农业部于2004年开始启动保护性措施效益评价项目(CEAP),以定量评估保护性措施在区域尺度上的效益。通过对CEAP的研究背景、目的和方法的介绍,并对评估指标、数据采集方法和采用的主要模型作了说明,进而为我国水土保持环境效益评估提出建议。 展开更多
关键词 农业 保护性措施效益评价 评估指标 数据采集
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适用于同塔四回输电线路的保护性能评估系统 被引量:8
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作者 李晓华 刘飘 +3 位作者 蔡旺延 李帆 张少凡 曹杰 《电力科学与技术学报》 CAS 北大核心 2019年第1期59-66,共8页
输电线路同塔四回线路架设日益普遍,线间复杂的电磁耦合关系给继电保护整定计算与电网安全运行带来了新的挑战。在此背景下,设计一种同塔四回输电线路保护性能评估系统,系统主体结构涵盖当前同塔四回输电线路的所有架设形式,并能灵活可... 输电线路同塔四回线路架设日益普遍,线间复杂的电磁耦合关系给继电保护整定计算与电网安全运行带来了新的挑战。在此背景下,设计一种同塔四回输电线路保护性能评估系统,系统主体结构涵盖当前同塔四回输电线路的所有架设形式,并能灵活可调以适应实际线路结构。输入线路结构参数、接入电网信息等数据后,评估系统自动进行故障扫描,根据各种故障情形下保护的动作情况进行加权计算,获得对应的保护性能评估值,给出最恶劣情况下保护系统的安全性评估结果,并进一步对保护整定的合理性、新型保护原理或对策的实用性、系统运行及线路检修方式的安全性等进行合理评估。 展开更多
关键词 继电保护 同塔输电线路 可靠性评估 保护性评价
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Assessment of Diversity, Distribution, Conservation Status and Preparation of Management Plan for Medicinal Plants in the Catchment Area of Parbati Hydroelectric Project Stage-Ⅲ in Northwestern Himalaya 被引量:4
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作者 S.S. Samant Jitendra S. Butola Aman Sharma 《Journal of Mountain Science》 SCIE CSCD 2007年第1期34-56,共23页
The developmental activities, particularly the construction of hydroelectric projects are causing a great loss of biodiversity in the Indian Himalayan Region. The Himaehal Pradesh, a part of IHR is well known for the ... The developmental activities, particularly the construction of hydroelectric projects are causing a great loss of biodiversity in the Indian Himalayan Region. The Himaehal Pradesh, a part of IHR is well known for the development of hydroelectric projects. The Parbati H.E. Project is amongst the major projects of the State. The different stages of the project are all causing loss of biodiversity of the area. Stage Ⅲ of the Parbati H.E. Project is a run of the river scheme on the Sainj River downstream of Power House of Parbati H.E. Project Stage Ⅱ. The project shall utilize regulated discharge of Parbati H.E. Project Stage Ⅱ and inflow of River Sainj for power generation, and has been contemplated as a peaking station operating in tandem with Stage Ⅱ. The present study has been undertaken to see the impact of hydroelectric project on the biodiversity, particularly on medicinal plants. A total of 104 species of medicinal plants, belonging to different life forms, i.e., trees (23 spp.), shrubs (22 spp.), herbs (57 spp.) and ferns (2 spp.) were recorded. The species have been analyzed and studied for their distribution, classification, altitudinal zones, part (s) used, indigenous uses, nativity, endemism and rarity.Different parts of these species, such as whole plants, roots (including rhizomes and tubers), leaves, flowers, fruits, seeds, stems, barks, spikes, nuts and insect galls are used by the inhabitants for curing various diseases and ailments. 3o species are native to the Himalayan region, 9 species native to the Himalayan region and adjacent countries also and 65 species are non-natives. 9 species are near endemics. Considering the whole Himalaya as a biogeographie unit (sensu lato), the near endemics are endemic to the Himalaya. Among these species, Zanthoxylum armature is categorized as Endangered and Valeriana wallichii as Vulnerable. Hedychium spicatum, Rhus javanica, Berberis lycium, Thalictrum foliolossum, Salvia lanata, Rubia cordifolia and Bergenia ligulata may be considered as threatened species due to their over exploitation for trade. 90 species are propagated by seeds, 8 species by seeds and rhizomes/roots/tubers, 4 species by seeds and cuttings, and 2 species by sori. A management plan for the cultivation and conservation of the medicinal plants in the dam submergence area, and the commercially viable medicinal plants with high value in the catchment area is suggested. 展开更多
关键词 Hydroelectric project dam submergence area management plan medicinal plant DIVERSITY native endemic ENDANGERED conservation status indigenous uses Indian Himalaya
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Short-term deformation behavior model of endangered earthen heritage slope after conservation in Jiaohe Ruins
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作者 张景科 谌文武 和法国 《Journal of Central South University》 SCIE EI CAS 2012年第7期2029-2036,共8页
Cliff deformation behavior after conservation is of great significance for evaluating the conservation effect and discovering the dynamical law of soil. Modeling on deformation behavior is beneficial to the quantitati... Cliff deformation behavior after conservation is of great significance for evaluating the conservation effect and discovering the dynamical law of soil. Modeling on deformation behavior is beneficial to the quantitative evaluation of interactions between soil mass and structures as well as the forecast. Based on cliff conservation engineering of Jiaohe Ruins (the largest raw soil heritage site in the world), data of horizontal deformation of the upper cliff were obtained by using Nanrui-made NDW-50 displacement device (precision: 0.01 mm, frequency: 15 min^-l). Regression analysis indicates that deformation behavior models include exponential growth, linear growth and parabolic growth types, while daily deformation presents more intense periodicity (24 h). The deformation is less than 1.5 mm during monitoring period, which has no impact on the stability of cliff. Deformation behavior provides the mutual duress and interaction between soil and engineering intervention. In addition, deformation mode attaches tensely to the damage pattern of the cliff. The conclusions are of importance to the stability evaluation of the carrier along Silk Road. 展开更多
关键词 endangered cliff CONSERVATION deformation behavior regression analysis modelling
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Evaluation of protective quality of prestressed concrete containment buildings of nuclear power plants
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作者 郑砚国 李惠强 《Journal of Central South University》 SCIE EI CAS 2011年第1期238-243,共6页
The permeability and sorptivity properties of the two prestressed concrete containment buildings (PCCBs) of a nuclear power plant in South China, which had been under operation for 5 years, were measured by using th... The permeability and sorptivity properties of the two prestressed concrete containment buildings (PCCBs) of a nuclear power plant in South China, which had been under operation for 5 years, were measured by using the autoclam permeability system. The air permeability, sorptivity and water permeability indexes of No.1 PCCB are smaller than or equal to 0.11 ln(102 Pa)/min, 0.98×10 ^7 m3/minl/2 and 1.93×10 ^7 m3/min1/2, respectively, and the air permeability, sorptivity and water permeability indexes of No.2 PCCB are smaller than or equal to 0.17 In(102 Pa)/min, 1.6×10 ^7 m3/min1/2 and 4.43 ×10 ^7 m3/min1/2, respectively. Based on the criteria for evaluating the protective quality of concrete structures in terms of their permeability and sorptivity properties, proposed by the research organization of the autoclam permeability system, the protective quality of No. 1 PCCB is still in very good grade and that of No.2 PCCB is not in very good grade but in good grade, and the in-service inspection of the protective quality of No.2 PCCB should be strengthened in the future. 展开更多
关键词 nuclear power plant prestressed concrete containment building permeability properties sorptivity properties
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Economic Value of Crop Productivity Biodiversity in ljebu Ode Agricultural Zone of Ogun State, Nigeria
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作者 Ayinde Alani Ezekiel Igbekele Amos Ajibefun +1 位作者 Taiwo Nuraeni Akinniran Folayimi Ogunwole-Olapade 《Sociology Study》 2016年第2期111-123,共13页
Biological diversity involves genetic, species, and ecosystem diversity. Genetic diversity denotes the variation within species in the functional units of heredity present in any plant or animal, microbial or other or... Biological diversity involves genetic, species, and ecosystem diversity. Genetic diversity denotes the variation within species in the functional units of heredity present in any plant or animal, microbial or other origin of living things. Species diversity encompasses the variety of species--whether wild or domesticated, within a geographical area. This study assesses the economic value of crop productivity biodiversity in Ijebu Ode agricultural zone of Ogun State. The authors proposed a dynamic model of market equilibrium with vertical product differentiation that enabled us to take into account the economic consequences of respondents' monthly income and species crop of three different communities. The objectives of the study are to identify the personal and socio-economic characteristics of farmers and determine the importance of biodiversity in the study area using Simpson's Index. The result showed that 52.50% were male, majority (32.50%) were within the age range of 31 and 40 years old, 44.17% were married, 62.50% had household size of 6-10, and majority (45.83%) had secondary education while 46.67% had up to 20 years farming experience. In conclusion, the authors find that effective factors play an important role in explaining the support for biodiversity conservation, particularly when people do not have specific knowledge about species; while ecological-scientific considerations appear when people are well-informed. 展开更多
关键词 Economic value CROP PRODUCTIVITY BIODIVERSITY CONSUMERS
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A procedural study of building up a performance evaluation indicator system of intellectual property protection
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作者 Luo Juan Xu Xingxiang 《International Journal of Technology Management》 2015年第10期113-116,共4页
The intellectual property protection, whether judicial or administrative, is evaluated through a performance evaluation indicator system. To building up such a system, we must follow certain working procedures which u... The intellectual property protection, whether judicial or administrative, is evaluated through a performance evaluation indicator system. To building up such a system, we must follow certain working procedures which usually consist of four steps: to determine the performance objectives, to design the structure of indicator system, to specify the indicators and to set up the weight of indicators. Each step plays a different role in performance evaluation indicator system and has its own impact on the realization of performance evaluation objectives respectively. So the scientifically building up a performance evaluation indicator system is the key to determine whether the intellectual property is protected well or not. 展开更多
关键词 lntellectual property protection performance evaluation indicator system performance objective evaluating indicators weights of indicators
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金黄色葡萄球菌荚膜多糖型CP8菌小鼠全身感染模型的建立及评价 被引量:3
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作者 袁玉兰 郭京蓉 +2 位作者 林海涛 周继唯 张云涛 《中国生物制品学杂志》 CAS CSCD 2019年第11期1228-1233,共6页
目的建立金黄色葡萄球菌(简称金葡菌)荚膜多糖型CP8菌小鼠全身感染模型,并对模型进行免疫攻毒保护性评价。方法金葡菌荚膜多糖型CP8国际标准株49525经37℃培养24 h,用PBS缓冲液稀释成不同浓度菌液,分组经腹腔注射感染BALB/c小鼠,观察小... 目的建立金黄色葡萄球菌(简称金葡菌)荚膜多糖型CP8菌小鼠全身感染模型,并对模型进行免疫攻毒保护性评价。方法金葡菌荚膜多糖型CP8国际标准株49525经37℃培养24 h,用PBS缓冲液稀释成不同浓度菌液,分组经腹腔注射感染BALB/c小鼠,观察小鼠发病症状,统计生存率及体重变化,确定每只小鼠的致死剂量及亚致死剂量;采集不同感染时间的小鼠血液及心脏、肝脏、脾脏、肺、肾脏,进行细菌定植量检测及主要脏器病理学检查。用CP8多糖-蛋白偶联物(实验组)及氢氧化铝佐剂(对照组)对建立的小鼠模型进行3次免疫攻毒保护性实验,统计小鼠存活率。结果经腹腔感染途径建立的小鼠模型,每只致死剂量为3.0×10^9 CFU,亚致死剂量为1.5×10^9 CFU。感染发病小鼠表现为翘毛弓背、耸肩及行动迟缓,生存率及体重随感染剂量增加明显下降,血液及心脏、肝脏、脾脏、肺、肾脏均有细菌定植,心脏、肾脏、肝脏主要脏器中可见明显细胞坏死和炎性细胞浸润。与对照组比较,实验组小鼠3次攻毒存活率差异均有统计学意义(P均<0.01),对小鼠的保护率分别为55.6%、50.0%和57.1%。结论成功建立金葡菌荚膜多糖型CP8菌小鼠全身感染模型,并可用于疫苗保护性评价,为进一步研究多抗原组分疫苗的有效性和安全性奠定了实验基础。 展开更多
关键词 金黄色葡萄球菌 荚膜多糖型CP8菌 全身感染 小鼠模型 保护性评价
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Assessment of effectiveness of nature reserves on the Tibetan Plateau based on net primary production and the large sample comparison method 被引量:6
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作者 张镱锂 胡忠俊 +7 位作者 祁威 吴雪 摆万奇 李兰晖 丁明军 刘林山 王兆锋 郑度 《Journal of Geographical Sciences》 SCIE CSCD 2016年第1期27-44,共18页
Twenty-one typical coupled large samples were chosen from areas within and surrounding nature reserves on the Tibetan Plateau using the large sample comparison method(LSCM).To evaluate the effectiveness of the nature ... Twenty-one typical coupled large samples were chosen from areas within and surrounding nature reserves on the Tibetan Plateau using the large sample comparison method(LSCM).To evaluate the effectiveness of the nature reserves in protecting the ecological environment,the alpine grassland net primary production(NPP) of these coupled samples were compared and the differences between them before and after their establishment as protected areas were analyzed.The results showed that:(1) With respect to the alpine grassland NPP,the ecological and environmental conditions of most nature reserves were more fragile than those of the surrounding areas and also lower than the average values for the Tibetan Plateau.(2) Of the 11 typical nature reserves selected,the positive trend in the NPP for Manzetang was the most significant,whereas there was no obvious trend in Taxkorgan.With the exception of Selincuo,the annual NPP growth rate in the nature reserves covered by alpine meadow and wetland was higher than that in nature reserves consisting of alpine steppe and alpine desert.(3) There were notable findings in 21 typical coupled samples:(a) After the establishment of the nature reserves,the annual rate of increase in the NPP in 76% of samples inside nature reserves and 82% of samples inside national nature reserves was higher than that of the corresponding samples outside nature reserves.(b) The effectiveness of ecological protection of the Mid-Kunlun,Changshagongma,Zoige and Selincuo(Selin Co) nature reserves was significant; the effectiveness of protection was relatively sig-nificant in most parts of the Sanjiangyuan and Qiangtang nature reserves,whereas in south-east Manzetang and north Taxkorgan the protection effectiveness was not obvious.(c) The ecological protection effectiveness was significant in nature reserves consisting of alpine meadow,but was weak in nature reserves covered by alpine steppe.This study also shows that the advantage of large sample comparison method in evaluating regional ecology change.Careful design of the samples used,to ensure comparability between the samples,is crucial to the success of this LSCM. 展开更多
关键词 nature reserves protection effectiveness large sample comparison method net primary production Tibetan Plateau
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