Trumpet shell, Charonia sauliae, is an endangered and valuable species, but its artificial propagation protocol has not been successfully established. To estimate the possibility of cryopreservation for larvae of C. s...Trumpet shell, Charonia sauliae, is an endangered and valuable species, but its artificial propagation protocol has not been successfully established. To estimate the possibility of cryopreservation for larvae of C. sauliae, which is a potential preparation for its artificial reproduction and further research, in this study a protocoi for the cryopreservation of veliger larvae of trumpet shell was optimized. Through a two-step cryopreservation procedure, four kinds of cryoprotectants （ethylene glycol, 1, 2-propanediol, dimethyl sulfoxide and glycerol） were employed at three concentrations （1.0, 1.5 and 2.0molL^-1） respectively and survival rates of larvae were determined after a storage of lh. The larvae frozen with these four cryoprotectants after 1 h storage were cultured, and then survival rates were determined at 24, 72 and 120 h after thawing. Dimethyl sulfoxide at a concentration of 1.5 molL^-1 showed the best protective effect in all experiments （p〈0.05）. And survival rates of larvae frozen with dimethyl sulfoxide were determined after 1, 7 and 15 d of storage. The survival rates of larvae frozen with 1.5 molL^-1 dimethyl sulfoxide after 1 h, 1 d, 7 d and 15 d of storage were 80.77% ±7.51%, 80.34%±11.28%, 83.10%±9.14% and 77.23%±6.22% respectively. No significant differences in survival rates of larvae frozen with dimethyl sulfoxide were observed after various storage periods （p〉0.05）.展开更多
The trade creation and conservation of wild species in Amazon, including collared peccaries （Pecari tajacu）, may be favored by artificial insemination, but the time of semen storage may lead to reduced fertility in ...The trade creation and conservation of wild species in Amazon, including collared peccaries （Pecari tajacu）, may be favored by artificial insemination, but the time of semen storage may lead to reduced fertility in sperm of some wild animals. Thus, the aim of this research was to evaluate the influence of semen storage at 17 ℃ over time on seminal features of peccaries in captivity. Eight adult males were sedated and underwent electroejaculation. The ejaculates （n = 65） were evaluated for volume, concentration, pH, sperm motility, vigor, and cell with intact plasma membrane intact （IPM） and sperm morphology. Selected ejaculates （n = 21） were diluted （1：1） in Beltsville Thawing Solution and kept during 48 hours under controlled temperature （17 ℃）. Assessments were made after dilution （TO）, after 24 hours （T24）, and 48 hours after the onset of cooling （T48）. The storage impacted on sperm survival （P 〈 0.05）. Semen characteristics changed throughout the storage period studied and after 48 hours storage. The decline of sperm motility was of 55.2% for 10.9%, vigor was 2.3 for 0.5 and IPM cells were of 59.0% for 15.8%. Primary defects sperm increased of 19.8% for 32.2%, secondary defects of 9.8% for 10.4% and total defects of 29.4% for 42.7%. However, within 24 hours of preservation, chilled semen peccaries presented sperm motility average rate and IMP cells levels indicative to use in assisted reproductive events. These results indicate chilled semen for 24 hours could be used in experimentally artificial insemination of peccaries, technology that still has not been performed before.展开更多
It is generally accepted that eggs besides being a source of everyday diet, can also be used as raw material for special food, nutraceutical, pharmaceutical and cosmetic production. The biological activity of the majo...It is generally accepted that eggs besides being a source of everyday diet, can also be used as raw material for special food, nutraceutical, pharmaceutical and cosmetic production. The biological activity of the majority of substances presented in egg matter is related to their anti-microbial and anti-cancer properties and their immunogenic features as well. Due to the use of new generation of feed additives and techniques of immunisation of hens, the chemical composition and biological features of eggs may be modified and enriched with desired components, like n-3 fatty acids （alpha-linolenic acid （ALA）, docosahexaenoic acid （DHA） phospholipids）, vitamins, microelements. Egg material may also be used for the production of new biopreparations for the prophylaxis of various civilisation diseases, such as heart and cardiovascular diseases, cancers, neurogenerative disorders, metabolic bone diseases etc.. New progress in egg science allowed to discover new bioactive substances, like monomeric and dimeric cystatin and yolkin. It is necessary to develop efficient methods of isolation of biologically active substances contained in eggs, which would make it possible to produce new generation of diet supplements and biomedical products. It seems that new generation of eggs could be the best source for production of natural, high value nutraceutical supplements of diet, and also biomedical and biocosmetic preparations.展开更多
Extra-cellular compounds, secreted by microorganisms into their surroundings, can be integrated in concrete composition as admixtures. These substances are important in biofilm formation and some of them can be used a...Extra-cellular compounds, secreted by microorganisms into their surroundings, can be integrated in concrete composition as admixtures. These substances are important in biofilm formation and some of them can be used as corrosion inhibitor of concrete reinforcement. This paper deals with products made with biological surface active compounds/agents allowing the development of more eco-friendly concrete. The influence of this environmentally friendly bio admixture on setting time, workability, bending and compressive strengths of various mortar based materials made of CEM I, CEM III and CEM V was studied. Mechanical tests were carried out to highlight the influence of admixture in workability and hardening of samples containing the biological product with ratio in the range of 0-2.5%. It was demonstrated that the presence of the new bio-compound admixture in mortar decreases their compressive strength after 28 days of standard curing, in spite of remaining higher than standard minimal strength. Furthermore, Vicat needle experiments have shown a tendency of this admixture to decrease the setting time. A discussion was finally proposed in order to correlate the setting times and the decrease of the mortar compressive strength, corresponding in fact to a hardening delay. This setting time delay could be linked to a delay of the admixtured mortar to increase its resistance. The slump results highlight the action of bio-admixture as a plasticizer on mortars because it increases their workability for a same water-cement ratio. This effect seems variable according to the added amount.展开更多
文摘Trumpet shell, Charonia sauliae, is an endangered and valuable species, but its artificial propagation protocol has not been successfully established. To estimate the possibility of cryopreservation for larvae of C. sauliae, which is a potential preparation for its artificial reproduction and further research, in this study a protocoi for the cryopreservation of veliger larvae of trumpet shell was optimized. Through a two-step cryopreservation procedure, four kinds of cryoprotectants （ethylene glycol, 1, 2-propanediol, dimethyl sulfoxide and glycerol） were employed at three concentrations （1.0, 1.5 and 2.0molL^-1） respectively and survival rates of larvae were determined after a storage of lh. The larvae frozen with these four cryoprotectants after 1 h storage were cultured, and then survival rates were determined at 24, 72 and 120 h after thawing. Dimethyl sulfoxide at a concentration of 1.5 molL^-1 showed the best protective effect in all experiments （p〈0.05）. And survival rates of larvae frozen with dimethyl sulfoxide were determined after 1, 7 and 15 d of storage. The survival rates of larvae frozen with 1.5 molL^-1 dimethyl sulfoxide after 1 h, 1 d, 7 d and 15 d of storage were 80.77% ±7.51%, 80.34%±11.28%, 83.10%±9.14% and 77.23%±6.22% respectively. No significant differences in survival rates of larvae frozen with dimethyl sulfoxide were observed after various storage periods （p〉0.05）.
文摘The trade creation and conservation of wild species in Amazon, including collared peccaries （Pecari tajacu）, may be favored by artificial insemination, but the time of semen storage may lead to reduced fertility in sperm of some wild animals. Thus, the aim of this research was to evaluate the influence of semen storage at 17 ℃ over time on seminal features of peccaries in captivity. Eight adult males were sedated and underwent electroejaculation. The ejaculates （n = 65） were evaluated for volume, concentration, pH, sperm motility, vigor, and cell with intact plasma membrane intact （IPM） and sperm morphology. Selected ejaculates （n = 21） were diluted （1：1） in Beltsville Thawing Solution and kept during 48 hours under controlled temperature （17 ℃）. Assessments were made after dilution （TO）, after 24 hours （T24）, and 48 hours after the onset of cooling （T48）. The storage impacted on sperm survival （P 〈 0.05）. Semen characteristics changed throughout the storage period studied and after 48 hours storage. The decline of sperm motility was of 55.2% for 10.9%, vigor was 2.3 for 0.5 and IPM cells were of 59.0% for 15.8%. Primary defects sperm increased of 19.8% for 32.2%, secondary defects of 9.8% for 10.4% and total defects of 29.4% for 42.7%. However, within 24 hours of preservation, chilled semen peccaries presented sperm motility average rate and IMP cells levels indicative to use in assisted reproductive events. These results indicate chilled semen for 24 hours could be used in experimentally artificial insemination of peccaries, technology that still has not been performed before.
文摘It is generally accepted that eggs besides being a source of everyday diet, can also be used as raw material for special food, nutraceutical, pharmaceutical and cosmetic production. The biological activity of the majority of substances presented in egg matter is related to their anti-microbial and anti-cancer properties and their immunogenic features as well. Due to the use of new generation of feed additives and techniques of immunisation of hens, the chemical composition and biological features of eggs may be modified and enriched with desired components, like n-3 fatty acids （alpha-linolenic acid （ALA）, docosahexaenoic acid （DHA） phospholipids）, vitamins, microelements. Egg material may also be used for the production of new biopreparations for the prophylaxis of various civilisation diseases, such as heart and cardiovascular diseases, cancers, neurogenerative disorders, metabolic bone diseases etc.. New progress in egg science allowed to discover new bioactive substances, like monomeric and dimeric cystatin and yolkin. It is necessary to develop efficient methods of isolation of biologically active substances contained in eggs, which would make it possible to produce new generation of diet supplements and biomedical products. It seems that new generation of eggs could be the best source for production of natural, high value nutraceutical supplements of diet, and also biomedical and biocosmetic preparations.
文摘Extra-cellular compounds, secreted by microorganisms into their surroundings, can be integrated in concrete composition as admixtures. These substances are important in biofilm formation and some of them can be used as corrosion inhibitor of concrete reinforcement. This paper deals with products made with biological surface active compounds/agents allowing the development of more eco-friendly concrete. The influence of this environmentally friendly bio admixture on setting time, workability, bending and compressive strengths of various mortar based materials made of CEM I, CEM III and CEM V was studied. Mechanical tests were carried out to highlight the influence of admixture in workability and hardening of samples containing the biological product with ratio in the range of 0-2.5%. It was demonstrated that the presence of the new bio-compound admixture in mortar decreases their compressive strength after 28 days of standard curing, in spite of remaining higher than standard minimal strength. Furthermore, Vicat needle experiments have shown a tendency of this admixture to decrease the setting time. A discussion was finally proposed in order to correlate the setting times and the decrease of the mortar compressive strength, corresponding in fact to a hardening delay. This setting time delay could be linked to a delay of the admixtured mortar to increase its resistance. The slump results highlight the action of bio-admixture as a plasticizer on mortars because it increases their workability for a same water-cement ratio. This effect seems variable according to the added amount.
