全营养天然脱腥保溶大豆蛋白粉

在植物蛋白开发利用中,研究得最深入,应用最广泛,最丰富的莫过于大豆蛋白。在大豆提油后的饼粕中含有50％以上的蛋白质,比瘦肉高二点八倍,比鸡蛋高三点五倍。其蛋白质中有含有人体必需的八种氨基酸,特别是赖氨酸、天门冬氨酸。

脱腥保溶大豆蛋白粉的研究

本文论述了全营养天然脱腥保溶大豆蛋白粉的生产原理、工艺流程、产品性能和质量分析,以及该产品的应用范围及经济效益等问题。

Anti-tumor Effect and Protective Effect on Chemotherapeutic Damage of Water Soluble Extracts from Hedyotis diffusa

Bai-Hua-She-She-Cao Hedyotis diffusa Willd. (Ru-biaceae) is a medicinal herbwidely distributed in northeast Asian countries. In traditional Chinese medicine, it has the effectof 'clearing away heat and toxic material, promoting blood circulation and removing blood stasis'.It is a well known Chinese folk-medicine used for the treatment of appendicitis, sore throat, mumps,acne, sebo-rheic dermatitis and various kinds of tumors, such as tumors of digestive tract,carcinoma of liver. It was reported that the MeOH extract of H. diffusa demonstrated a significantantitumor activity and ursolic acid succeeded in being isolated from the MeOH extract as an activecomponent . Shan BN, et al suggested that the direct aqueous extract of H. diffusa hadimmuno-modulating activity and antitumor activity in vitro through stimulating the immune system tokill or engulf tumor cells. But regarding anti-tumor activity in vivo of water soluble extracts fromH. diffusa, no detail was reported. Therefore, we prepared water soluble extracts (H_1 and H_2)from H. diffusa and evaluated their anti-tumor property in vivo experiments as well as protectiveeffect on chemo-therapeutic damage.

Evaluation of CMU-1 preservation solutions using an isolated perfused rat liver model

AIM: CMU-1 is a new preservation solution with a low potassium concentration as well as low viscosity that is highly effective in reducing preservation injury. The purpose of this experiment is to compare the protective effect of CMU-1 solution with that of UW during cold preservation and normothermic reperfusion. METHODS: Wistar rats were divided into two groups according to different preservation solution: CMU-1 group and UW group. After 6, 12 and 24 h cold storage of rat liver in different preservation solutions, the isolated perfused rat liver model was applied to reperfuse the liver for 120 min normothermically (37 癈) with Krebs-Henseleit solution, meanwhile the pH value of the preservation solution was measured. The perfusate was sampled for the evaluation of alanine aminotransferase (ALT) and lactate dehydrogenase (LDH). At the end of the reperfusion, all of the bile product was collected, energy metabolic substrate and histological examination were performed. RESULTS: After preserving for 6 h, pH value of both groups did not change; after 12 h, both decreased but with no significant difference. After 24 h, pH value in UW solution group significantly decreased. The total adenine nucleotides level and AEC in liver tissue decreased with preservation time, but they were higher in CMU-1 group. And the amount of bile product after perfusion for 120 min in CMU-1 group was much more than that in UW group. However, there were no significant differences in ALT and LDH levels between two groups. Histology showed no difference. CONCLUSION: The preservation effect of CMU-1 solution is similar with that of UW solution. However, CMU-1 solution shows some advantages over UW solution in energy metabolism, preventing intracellular acidosis and bile product.

环保型特种溶剂油的市场及竞争力分析

本文重点介绍了国内环保型特种溶剂油的产品特点和市场现状,认为生产环保型特种溶剂油可以提高产品档次和附加值,增加企业经济效益。

Characterization and photoelectrochemical performance of Zn-doped TiO_2 films by sol-gel method

Zn-doped TiO2 (Zn?TiO2) thin films were prepared by the sol?gel method on titanium substrates with heat treatment at different temperatures. The effects of heat treatment temperatures and Zn doping on the structure, photocathodic protection and photoelectrochemical properties of TiO2 thin films were investigated. It is indicated that the photoelectrical performance of the Zn?TiO2 films is enhanced with the addition of Zn element compared with the pure-TiO2 film and the largest decline by 897 mV in the electrode potential is achieved under 300 °C heat treatment. SEM?EDS analyses show that Zn element is unevenly distributed in Zn?TiO2 films; XRD patterns reveal that the grain size of Zn?TiO2 is smaller than that of pure-TiO2; FTIR results indicate that Zn - O bond forms on Zn?TiO2 surface. Ultraviolet visible absorption spectra prove that Zn?TiO2 shifts to visible light region.Mott?Shottky curves show that the flat-band potential of Zn?TiO2 is more negative and charge carrier density is bigger than that ofpure-TiO2, implying that under the synergy of the width of the space-charge layer, carrier density and flat-band potential, Zn?TiO2 with 300 °C heat treatment displays the best photocathodic protection performance.

Global Solution for Preserving Citrus Fruit Using Natural Treatments

Different tests were carried out to assess the efficiency of replacing plant protection products used at the main application points in citrus fruit packinghouses with natural treatments. The efficiency of an aqueous treatment containing potassium sorbate was found to significantly reduce decaying in Marisol mandarins previously inoculated with Penicillium digitatum and Penicillium italicum. The same food preservative was used in conjunction with a fruit detergent and was applied on Washington Navel oranges and the reduction in the appearance of decaying was similar to that obtained using Guazatine. Finally, two different essential oils were tested. One was combined with a coating and the KS, whilst the other was applied as a fumigant. In the case of wax treatments, it was found that the combination of essential oil or KS with Imazalil at half the dose （0.1%） was equivalent to treatment with a higher dose of Imazalil （0.2%） thus making it possible to reduce the dose of plant protection products used without reducing decay control effectiveness. The treatment with a fumigant with essential oil has a level of efficiency similar to Imazalil against PI. These treatments can be considered a viable alternative to the use of plant protection products.

Alginate Encapsulation as a Preservation Method of Pitaya Fruit Juice （Stenocereus spp.）

Alginate is a widely used polymer matrix in food industry since it allows formation of spherical, soft, and strong membranes adequate for encapsulation of a large amount of products, including food. The flow rate of alginate solutions and the permeability of the capsules were evaluated within an acidic-low acidic pH range and different alginate concentrations. In solutions adjusted at different pH （3.0 to 7.0） with concentrations of alginate of 0.8, 1.0, and 1.2% w/v, flow rates at 20 ℃ were 6.95 to 10.00, 4.54-5.35, and 2.60-2.80 mL sl, respectively. Permeability of the capsules was evaluated in terms of the diffusion of H＋ions （expressed as pH） and soluble solids （~Brix）. Meanwhile both diffusions were minor at 4.0 〈 pH 〈 7.0 and were significantly superior at more acidic pH （P 〈 0.05）, alginate concentration did not present significant effect. Yellow, purple, and red juices from Stenocereus spp. fruits （pitayas） were encapsulated using 1.0% of alginate and stored with isotonic solution （3 mL g^-1） at 4 ℃ in the dark. The capsules were spherical with diameter between 4.59 and 470 mm, weight from 82.60 to 97.50 rag, and volume of 0.075-0.098 mL. Pigment （total betalains content） diffusion reached equilibrium at 24 h of storage, at which point retentions of total betalains in the yellow, purple, and red capsules were 87.79, 96.13, and 85.13%, respectively. Also, changes in the color of the capsules were observed during storage.

A Simple Bio-preservation Technique to Increase Shelf Life of Ampalavi Mango Fruits Using Aloe vera Gel

Aloe vera is being identified as a potential medicinal plant for its application in industries as well as traditional usage. The gel obtained from the leaves of A. vera has numerous properties. In this study, using the gel to extend the shelf life of Ampalavi mango fruits was studied. Even sized, uniform coloured, matured Ampalavi cultivar mango fruits were surface cleaned and coated with 33%, 66% and 100% gel, respectively. Results revealed that the ripening was delayed due to the coating. The total soluble solid （TSS）, pH and weight loss were high in uncoated fruits. The mean pH of the pulp from fruits kept as control was 4.94 at 4 d fruit preservation period （FPP） and was slightly increased to 5.43 within 12 d FPP, whereas the minimal pH （4.69 at 4 d FPP and 5.03 at 9 d FPP） was noticed in 100% gel coated fruits. The TSS （brix） was significantly in higher levels （13.67°Bx within 4 d FPP and 20.77°Bx within 12 d FPP） in control fruits, whereas the minimum TSS value was 9.27°Bx and 18.03° Bx within 4 d and 12 d FPP, respectively, recorded from the 100% gel coated fruits in storage. The weight loss percentage （WLP） was significantly （P 〈 0.05） higher in control fruits （8.46%）, whereas the lower WLP （1.13%） was found in 100% gel coated fruits after 12 d of storage. This low-scale gel coating technique prolonged the fruits shelf life by delaying the fruit ripening. This effect has to be further investigated to commercialize the natural product for large scale ready-made application.

Screening and Preservation of Douchi Fibrinolytic Enzyme Producing Strain

[Objective] To isolate and preserve a high-activity Douchi fibrinolytic enzyme producing strain from Douchi products. [Method] The Douchi flbrinolytic enzyme producing strain was screened on the selected medium prepared with self-made pork blood powder, the strain with the highest activity was screened out according to the size of hydrolysis cycle, and then preserved in LB medium. [ Rebait] A Douchi fibrinolytic enzyme producing strain with high thrombolytic activity was successfully screened out from the Douchi produced in Hunan, Guangdong and Jiangxi Prov- inces. [ Ceadusioe] The study lays foundation for the development of new-type thrombolytic drugs.

Cold-preserved human spermatozoa in electrolyte-free solution retain their pene-tration capacity

Objective: To evaluate penetration capacity of human sperm preserved in electrolyte-free (EF) solution at 4 ℃.Methods: The motility, acrosomal status penetration rate and fertility index of human sperm were assessed before and after cold-preservation in EF solution, respectively.Results: The motility of human sperm cold-preserved in EF solution for 1 week was significantly higher than that of human sperm cold-preserved in modified human tubal fluid (mHTF) (43.4%±7.9% vs 9.5%±2.5%, P<0.01 ).Although acrosomal status of human sperm cold-preserved in the EF solution before reinitiation was not different from those of the fresh sperm (capacitated sperm: 7.6%±1.8% vs 6.4±1.8%; acrosome-reacted sperm: 3.0%±1.7% vs 2.4±1.1%, P>0.05), the percentage of capacitated and acrosome-reacted sperm in the EF solution significantly increased after reinitiation (capacitated sperm: 16.0%±2.3% vs 7.6±1.8%, acrosome-reacted sperm: 9.4%±2.1% vs 3.0%±1.7%, P<0.01).The penetration rate and fertility index of cool-preserved human sperm in the EF solution were comparable with those of fresh sperm (48.1% vs 50.9%; 1.38±0.16 vs 1.29±0.13, respectively, P>0.05).Conclusion: Cold-preservation did not induce capacitation and acrosome reaction of human sperm in the EF solution, but human sperm cold-preserved in the EF solution for 1 week possesses as much penetration capacity as fresh sperm.

Freezing Desalination

The solidification of a binary mixture causes allowed thermosolutal convection. Under certain conditions, the separation of its components gives rise to a preferential movement of migration of solutes. The quantity and the concentration of the obtained products depend on the mechanisms of sepa＇,ration which depend on different parameters and the applied boundary conditions. In this way, the freezing of H20-NaC1 mixture makes it possible to obtain a liquid phase which is charged in solute and is called brine and a solid phase which is composed of ice being able to become fresh water, and so it can works as a desalination process. In this paper, a series of experiments of seawater freezing on cold surface is carried out at the laboratory under different operating conditions. During these experiments, the role of each parameter （temperature of crystallization, duration of crystallizat on...） which is able to impact the final composition is focused on. After a preliminary series of experiment, the yield is： improved to reach rates of salt and make it possible to regard the water produced as drinkable according to the standards of WHO （World Health Organization）.

Dimeric quinacridone cyclophanes: Synthesis,structures,and photophysical properties

Two novel quinacridone (QA) cyclophanes with intrinsic intramolecular dye-dye interactions have been designed and synthesized.X-ray crystal structures as well as detailed photophysical properties have been well demonstrated.These two dyes have a major advantage that efficient fluorescence quenching can be observed even in their dilute solutions.A comparison of photophysical properties between the dimeric QA cyclophane and its reference monomeric counterpart indicates that the dimerization is predominant for the fluorescence quenching of QA dyes in solution.This study provided some model QA derivatives with dimeric structures for understanding the fluorescence quenching of QA dyes in solutions.

An upper bound for the adiabatic approximation error

In this paper, we derive an upper bound for the adiabatic approximation error, which is the distance between the exact solution to a Schrodinger equation and the adiabatic approximation solution. As an application, we obtain an upper bound for 1 minus the fidelity of the exact solution and the adiabatic approximation solution to a SchrOdinger equation.

Design, preparation and activity evaluation of two novel proteins with thrombolysis or/and neuroprotection

We hypothesized that thrombolysis in combination with neuroprotection might have better therapeutic effects for ischemic stroke compared with thrombolysis alone.In order to verify such hypothesis,we designed a protein TBN by fusing NR6and BH4,which possibly had dual functions of thrombolysis and neuroprotection.NR6was obtained by introducing two RGD motifs to thrombolytic protein AcAP5to target thrombus.BH4is the key domain of anti-apoptotic protein Bcl-xL. The DNA fragments encoding TBN and NR6were synthesized and cloned into pET30a and pET16b vectors,respectively.Both proteins were expressed in E.coli.BL21(DE3),mainly in the form of inclusion bodies.With His-tag,NR6was purified by nickel affinity chromatography,while TBN was purified by ion exchange chromatography.Purified proteins were refolded by dialysis assay.The thrombolytic activity of both proteins was evaluated by the rat arteriovenous bypass model.Both NR6and TBN significantly reduced thrombus weight at higher dose (24nmol/kg),TBN showed similar effect to NR6.These results suggested that NR6was a thrombolytic protein,and fusion protein TBN reserved the thrombolytic activation of NR6.The effects of both proteins were also evaluated in thromboembolic middle cerebral artery occlusion (eMCAO)in mice.TBN exhibited better effect on reducing infarction volume and inhibiting apoptosis of cells than NR6,indicating that the introduction of BH4increased the protective effect of NR6.The hemorrhagic side effects of the two proteins were evaluated by tail bleeding in mice,and it was found that NR6and TBN showed shorter bleeding time compared with tPA.In conclusion,we designed and prepared the two novel proteins,and testified that they had.significant thrombolytic effect and protective effect on cerebral IR injury.The protective effect of TBN was more potent than NR6.Their bleeding side reaction might be weaker than tPA.These results suggested that these two novel proteins deserved to be further investigated as new thrombolytic candidate agents.

Photosensitive damage of lysozyme caused by pazufloxacin and the protective effect of ferulic acid

Laser flash photolysis and sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE) experiments were carried out to study the photosensitive damage induced by pazufloxacin(PAX) and the protection of lysozyme(Lyso) by ferulic acid(FCA), an active antioxidant. Transient absorption spectra revealed electron transfer from Lyso and FCA to 3PAX*, with respective bimolecular reaction rate constants of 6.3×107 and 1.4×1010 dm3/(mol s). A pulse radiolysis study was also performed to investigate the formation of Lyso radical cations. Results showed that FCA effectively inhibited the cross-linking of protein induced by fluoroquinolones. Finally, a mechanism of the protective effect of FCA on Lyso was proposed.