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低温冷冻伯氏疟原虫鼠疟原虫保种法
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作者 姚宝安 《中国人兽共患病杂志》 CSCD 北大核心 1995年第6期45-46,共2页
鼠疟原虫在疟疾实验研究中占有重要的地位。通常是采用含疟原虫血液经鼠转种或低温进行保种。但目前低温保种方法是取抗凝血加一定的保护剂后,低温保存。作者用含伯氏疟原虫的鼠不加保护剂,直接低温冷冻保存,获得了满意的结果,而且方法... 鼠疟原虫在疟疾实验研究中占有重要的地位。通常是采用含疟原虫血液经鼠转种或低温进行保种。但目前低温保种方法是取抗凝血加一定的保护剂后,低温保存。作者用含伯氏疟原虫的鼠不加保护剂,直接低温冷冻保存,获得了满意的结果,而且方法更简便,节约成本。 材料与方法 一、疟原虫 系湖北医科大学寄生虫学教研室经小白鼠保种的伯氏疟原虫(Plasmodiumberghei)正常株。 二、实验动物 昆明(KM)远交小白鼠,由湖北省医学科学院实验动物中心提供。经血检,无血液原虫。 三、实验方法 当转接小鼠RBC中疟原虫感染率达40%以上时,将鼠颈椎拉断迅速处死后,装入塑料袋内(1袋装1鼠),袋外标明日期,立即放入-70℃冰箱中保存。使用时,自冰箱中取出1只鼠,迅速投入40℃温水中解冻,15min后,将鼠以腹面向上固定,70%酒精消毒皮肤,剪开胸、腹腔,在注射器内先吸入0.9%灭菌盐水1.0ml,然后将针头插入右心室中抽取血液(如抽不出血液,先注入少量生理盐水再回抽)使血液混匀后立即由腹腔接种给健康小鼠2只,每只接种0.3ml。另外无菌取出小鼠肝脏置灭菌的乳钵中,加入0.9%灭菌生理盐水10ml.将肝组织研碎,稍静置,用注射器吸取上层悬液,由腹腔接种给小鼠2只,每只接种0.3ml。自第2d起,将被接种的鼠剪尾取血涂片。 展开更多
关键词 疟原虫 伯氏疟原虫 鼠疟原虫 保种法 低温冷冻
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小群保种方法的研究——狼山鸡(N系)血型基因种群结构分析 被引量:1
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作者 黄凡美 周勤宣 +2 位作者 张学余 苏一军 程光潮 《中国家禽》 北大核心 1990年第4期22-24,共3页
如何改进保存和管理畜禽遗传资源的方法,越来越得到国际的重视。联合国粮农组织曾于1980年6月在罗马召开《家畜遗传资源保存与管理的技术咨询会》,讨论、分析此问题。我国也已将畜禽品种资源的研究,正式列入“七五”期间重点攻关项目,
关键词 狼山鸡 保种法 血型基因 种群结构
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The Importance of Fiber Production for Conservation of Native Sheep and Goat Breeds and Silkworm Lines in Turkey
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作者 Gursel Dellal Zeynep Erdogan +2 位作者 Erkan Pehlivan Feryal Soylemezoglu Aysem Yanar 《Journal of Life Sciences》 2012年第7期826-831,共6页
In Turkey, studies devoted to conservation of animal genetic resources are carried out by general directorate of agricultural research (TAGEM) under the ministry of food, agriculture and livestock. There are 9 sheep... In Turkey, studies devoted to conservation of animal genetic resources are carried out by general directorate of agricultural research (TAGEM) under the ministry of food, agriculture and livestock. There are 9 sheep and 3 goat breeds and 3 silkworm lines which produce animal fiber have been put under conservation by TAGEM since 1996. A considerable amount of these genotypcs are preserved as in situ. The success of this conservation method is directly associated with economically making the best of these genotypes which are preserved. Hence, making the most of fiber production alongside meat and milk production would make a great contribution to their conservation. For this purpose, determining production biology of mohair and textile features of Ankara goats at present, determining all features of traditional items (especially handicrafts) made of fibers obtained from these breeds and lines which have been put under protection and creating new usage areas have been issues worked upon. Along with this, in recent years, just as the world, especially EU countries, interests and studies for issues such as organic animal fiber production, rural development and geographical indications have been increasing in Turkey, as well. In the near future, this situation is supposed to have a great effect on increasing of animal fiber production in a positive way, too. Hence, to benefit more effectively from fibers obtained from these breeds which have been put under protection will make a great contribution to be protected by breeders. For this purpose, there will be studies in the near future devoted to determining production biology of fiber and determining features of textile in breeds and lines and increasing the consumption of products to be obtained from these fibers as well as Ankara goats. Therefore, in this article, it was emphasized on the more efficient of ways to be benefited from fiber production in these breeds and lines under protected. 展开更多
关键词 SHEEP goat and silkworm genetic resources CONSERVATION fiber production.
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Landscape Network Approach to Assess Ecological Impacts of Road Projects on Biological Conservation 被引量:4
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作者 LIU Shiliang DENG Li +3 位作者 CHEN Liding LI Junran DONG Shikui ZHAO Haidi 《Chinese Geographical Science》 SCIE CSCD 2014年第1期5-14,共10页
Awareness of the adverse ecological effects of road and other large construction projects has resulted in a rapidly growing demand to quantitatively predict and evaluate the effects on gene flow among plants and anima... Awareness of the adverse ecological effects of road and other large construction projects has resulted in a rapidly growing demand to quantitatively predict and evaluate the effects on gene flow among plants and animals in natural habitats.In this study,we evaluated the effects of different road construction scenarios on animal species movement by using the methods of ecological landscape connectivity in the typical regions of Yunnan Province,China.The results showed landscape connectivity levels(Probability of Connectivity(PC))decreased after road construction and species with lower dispersal abilities were more affected to the subsequent habitat fragmentation.First level roads affected landscape connectivity most significantly.At a distance of 100 m,the PC index decreased more than 50%.Further analysis suggested that the use of landscape connectivity to assess landscape processes revealed the adverse effects on the species movement more effectively compared with the traditional landscape pattern analysis.In addition,we conducted a graph theory and a least-cost modeling discussion,it is obvious that they are powerful tools to represent and analyze landscape networks with respect to related species movement.Network analysis offers a quantitative and simple but effective tool for ecological process assessment and biological conservation on large scales. 展开更多
关键词 landscape connectivity road project landscape network graph theory least-cost model
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How territoriality affects the density of an egg predator: Habitat renovation and reintroduction as a method of conserving two endangered desert spring fish 被引量:1
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作者 Timothy PACIOREK Layla AL-SHAER Murray ITZKOWITZ 《Current Zoology》 SCIE CAS CSCD 2014年第4期527-533,共7页
Conservation of endangered sympatric species must be carefully executed to ensure that the protection of one species does not negatively impact the survival of another. Critically endangered Pecos gambusia Gambusia no... Conservation of endangered sympatric species must be carefully executed to ensure that the protection of one species does not negatively impact the survival of another. Critically endangered Pecos gambusia Gambusia nobilis and Leon Springs pupfish Cyprinodon bovinus, which are endemic to a threatened desert spring habitat, exemplify one such scenario. Recently, this habitat has been expanded, and captively bred C. bovinus were released to repopulate an area historically known to contain this species. A previous study suggested that G. nobilis are detrimental to C. bovinus populations, due to their tendency to congregate near spawning pairs and feed on their eggs. Whether G. nobilis are attracted to territorial C. bovinus, regardless of spawning behavior, remains unclear. To determine this, the number of G. nobilis in occupied and unoccupied breeding territories of both wild and captively bred C. bovinus was measured. Gambusia nobilis densities were similar between unoccupied and occupied territorial sites, suggesting that they do not show a preference for C. bovinus territories. Regardless of habitat location or prior captivity, territorial C. bovinus significantly excluded G. nobilis within their direct vicinity (5 cm), but not from their entire territory. Decreased G. nobilis density within the habitat may allow C. bovinus to defend their breeding territories more readily during the summer spawning season. This study provides empirical evidence of captively raised individuals behaving similarly to wild indi- viduals upon reintroduction to their natural habitat and support for conservation methods that focus on common limiting factors between endangered species [Current Zoology 60 (4): 527-533, 2014]. 展开更多
关键词 PUPFISH GAMBUSIA HABITAT Conservation Territory DENSITY
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