Objective To observe the clinical therapeutic effect on insomnia treated with acupuncture at back-shu points. Methods Sixty-four cases of insomnia were selected and divided randomly into a back-shu points group （grou...Objective To observe the clinical therapeutic effect on insomnia treated with acupuncture at back-shu points. Methods Sixty-four cases of insomnia were selected and divided randomly into a back-shu points group （group A） and a conventional acupoints group （group B） by a computer generated allocation list, 32 cases in each one. In the trial group, acupuncture was applied at xinshu （心俞 15）, Ganshu （刊俞 18）, Pishu （脾俞 BL 20） and Geshu （膈俞 BL 17）. In the control group, acupuncture was applied conventionally to Neiguan （内关 PC 6）, Shenmen （神门 HT 7）, Sanyinjiao （三阴交 SP 6）, Baihui （百会 GV 20） and Sishencong （四神聪 EX-HN 2）. Ten treatments made a session. The efficacy was analyzed statistically after 3 sessions of treatment. Results There were significant differences between the two groups in the relief of sleep disorders after the treatment （P〈0.O2）. The improvement in sleep efficiency was different significantly （P〈0.05）. The difference in sleep difficulty was significant in Chinese medicine （TCM） symptom score （P〈0.05）. Conclusion The clinical efficacy is achieved by either acupuncture at back-shu points or by acupuncture at conventional acupoints. The improvements of sleep efficiency and sleep disorder scores in PSO.I, and sleep difficulty in TCM as well as the long-term efficacy are superior in group A as compared with those in group B.展开更多
Objective： To observe the clinical effect of liver-regulating and blood-tonifying acupuncture in the treatment of cervical spondylosis of vertebral artery type. Methods： A total of 60 cases meeting the inclusion cri...Objective： To observe the clinical effect of liver-regulating and blood-tonifying acupuncture in the treatment of cervical spondylosis of vertebral artery type. Methods： A total of 60 cases meeting the inclusion criteria were randomized into an observation group and a control group, 30 cases in each group. Cases in the observation group received liver-regulating and blood-tonifying acupuncture, in which Jiaji （EX-B 2） points of C3_C7, Ganshu （BL 18） and Geshu （BL 17） were used; cases in the control group received routine acupuncture treatment in which jiaji （EX-B2） points of C3.C7 were used. Both groups received 5 times of treatment in a week with a 2-day rest, symptoms evaluation was done after 4 weeks of treatment, and then therapeutical effect was evaluated. Results： The total effective rate was 90.0% in the observation group, 73.3% in the control group, and inter-group comparison showed a statistical significance （P〈0.05）. After treatment, the symptoms and signs scores in both groups all substantially dropped, showing statistical significances （both P〈0.05）; inter-group comparison showed that the improvements in subscales of dizziness, pressing pain along vertebrae and revolve-cervix test in the observation group were superior than those in the control group, showing statistical significances （all P〈0.05）, while the inter-group comparisons of subscales of headache and vomiting and nause didn＇t show any statistical significances after treatment （both P〉0.05）. Conclusion： Liver-regulating and blood-tonifying acupuncture is effective in treating cervical spondylosis of vertebral artery type, and it can significantly improve patients＇ symptoms and signs.展开更多
Objective： To observe the effect of electroacupuncture （EA） on the expression of erythropoie-tin-producing hepatocyte receptor B2 （EphB2） in the cortex around the infracted area of middle cerebral artery occlusi...Objective： To observe the effect of electroacupuncture （EA） on the expression of erythropoie-tin-producing hepatocyte receptor B2 （EphB2） in the cortex around the infracted area of middle cerebral artery occlusion （MCAO） rats at different timing, and to reveal the possible mechanism of acupuncture in the treatment of cerebral ischemia. Methods： A total of 180 male Sprague-Dawley （SD） rats were randomly divided into a sham operation group, a model group, an acupoint group and a non-acupoint group, with 45 rats in each group. Rats in each group were further divided into three subgroups： postoperative 3 d, postoperative 14 d and postoperative 2l d groups, with 15 rats in each subgroup. The MCAO model was made by the modified occlusion method. The neurological function score, 2,3,5-triphenyl tetrazolium chloride （TFC） staining, immunohistochemistry assay, immunofluorescence double labeling method and Western blot were used to detect the corresponding indicators. Results： The neurological impairment of rats was most obvious at postoperative 3 d, and then gradually improved with time, which was more significant in the acupoint group （P〈0.05）. The change of infarcted volume was consistent with the neurological function impairment. The number of EphB2 positive cells （EphB2＋） around the infarcted area was decreased significantly at postoperative 3 d, and then gradually improved with time, which returned to the same level as that in the sham operation group at postoperative 21 d. The increase was most significant in the acupoint group （P〈0.05）, and the positive cell number was higher than that in the sham operation group （P〈0.01）. Western blot and immunohistochemistry results were basically consistent. Immunofluorescence displayed that EphB2＋ and postsynaptic density-95 positive （PSD-95＋） were co-expressed, after the MCAO operation, in the cortical neuron around the infracted area, and the number of co-expressing cells was increased gradually with time, which was most significant in the acupoint group （P〈0.05）. Conclusion： Electroacupuncture at Ganshu （BL 18） and Shenshu （BL 23） can significantly improve the neurological function and cerebral infarcted volume ratio of MCAO rats, which may be related to the activation of EphB2 expression in cortex around the infracted area and the promotion of synaptic remodeling.展开更多
Objective： To investigate the effect of electroacupuncture （EA） at Ganshu （BL 18） and Shenshu （BL 23） on vascular endothelial growth factor （VEGF） and platelet endothelial cell adhesion molecule-1 （PECAM-1）...Objective： To investigate the effect of electroacupuncture （EA） at Ganshu （BL 18） and Shenshu （BL 23） on vascular endothelial growth factor （VEGF） and platelet endothelial cell adhesion molecule-1 （PECAM-1）/CD31 around the cerebral infarction focus in middle cerebral artery occlusion （MCAO） rats and the possible mechanism, thus to provide a new strategy for the treatment of cerebral ischemic stroke by acupuncture. Methods： A total of 180 healthy male Sprague-Dawley （SD） rats were randomly divided into a sham operation group, a model group, an acupoint group and a non-acupoint group, 45 rats in each group. MCAO model was established using the modified line-embolus method in all rats except for those in the sham operation group; rats in the acupoint group were treated with EA at Ganshu （BL 18） and Shenshu （BL 23）; rats in the non-acupoint group were treated with EA at the control points; rats in other 2 groups were only subjected to bundling without treatment. Ten rats in each group were randomly selected on the 3rd day, the 14th day and the 21st day after acupuncture stimulation to test the neurological function impairment. The expression levels of CD31 and VEGF were also detected. Results： Compared with the model group and non-acupoint group, the neurological function score of the acupoint group was decreased at each time point, and the differences were statistically significant （P〈0.05, P〈0.01）. The expressions of VEGF and CD31 in each group were the lowest on the 3rd day, reached the peak on the 14th day and still remained at high level on the 21st day. And the differences among groups were statistically significant both on the 14th day and the 21st day （P〈0.05, P〈0.01）. Compared with the model group and the non-acupoint group, the expressions of VEGF and CD31 in the acupoint group were increased, and the differences were statistically significant （all P〈0.05）. Conclusion： EA at Ganshu （BL 18） and Shenshu （BL 23） can significantly improve the neurological function score of MCAO model rats, and shows protective effect on cerebral ischemia. The protective mechanism may be related to the up-regulation of CD31 and VEGF expression around the cerebral infarction focus in the MCAO model rats and induction of angiogenesis.展开更多
Objective:To observe the clinical efficacy of acupoint injection with salmon calcitonin for back pain in elderly patients with primary osteoporosis.Methods:A total of 76 patients were selected and randomly divided int...Objective:To observe the clinical efficacy of acupoint injection with salmon calcitonin for back pain in elderly patients with primary osteoporosis.Methods:A total of 76 patients were selected and randomly divided into two groups by the random number table method,with 39 cases in the treatment group and 37 cases in the control group,respectively.Patients in both groups received routine anti-osteoporosis treatment.Patients in the treatment group received additional acupoint injection with salmon calcitonin at bilateral Pishu(BL 20)and Shenshu(BL 23),while patients in the control group received additional intramuscular injection with salmon calcitonin.The treatments for both groups were given once a day and lasted for 4 weeks.Visual analog scale(VAS)and Chinese Oswestry disability index(CODI)scores were observed before treatment,after 2 weeks and 4 weeks of treatment,and the use of analgesics during the treatment were recorded.Results:After treatment,the clinical efficacy in the treatment group was more significant than that in the control group(P<0.05).After 2 weeks and 4 weeks of treatment,the VAS scores in both groups showed significant intra-group and between-group differences(all P<0.05),and the CODI scores in both groups showed significant intra-group differences(all P<0.05).After 2 weeks of treatment,the CODI score showed no significant between-group difference(P>0.05).After 4 weeks of treatment,the improvement of CODI score in the treatment group was more significant than that in the control group(P<0.05).During the treatment,2 cases in the treatment group took analgesics versus 8 cases in the control group,and the result showed a significant between-group difference(P<0.05).Conclusion:For back pain in elderly patients with primary osteoporosis,based on the routine treatment of oral medication,the clinical efficacy of acupoint injection with salmon calcitonin at bilateral Pishu(BL 20)and Shenshu(BL 23)is more significant than that of intramuscular injection.Acupoint injection treatment can improve patients'conditions and reduce the use of analgesics.展开更多
基金Supported by National Natural Science Foundation:No.81173335
文摘Objective To observe the clinical therapeutic effect on insomnia treated with acupuncture at back-shu points. Methods Sixty-four cases of insomnia were selected and divided randomly into a back-shu points group （group A） and a conventional acupoints group （group B） by a computer generated allocation list, 32 cases in each one. In the trial group, acupuncture was applied at xinshu （心俞 15）, Ganshu （刊俞 18）, Pishu （脾俞 BL 20） and Geshu （膈俞 BL 17）. In the control group, acupuncture was applied conventionally to Neiguan （内关 PC 6）, Shenmen （神门 HT 7）, Sanyinjiao （三阴交 SP 6）, Baihui （百会 GV 20） and Sishencong （四神聪 EX-HN 2）. Ten treatments made a session. The efficacy was analyzed statistically after 3 sessions of treatment. Results There were significant differences between the two groups in the relief of sleep disorders after the treatment （P〈0.O2）. The improvement in sleep efficiency was different significantly （P〈0.05）. The difference in sleep difficulty was significant in Chinese medicine （TCM） symptom score （P〈0.05）. Conclusion The clinical efficacy is achieved by either acupuncture at back-shu points or by acupuncture at conventional acupoints. The improvements of sleep efficiency and sleep disorder scores in PSO.I, and sleep difficulty in TCM as well as the long-term efficacy are superior in group A as compared with those in group B.
基金supported by Research and Development Project of Region Public Institution in Science and Technology Plan of Nanshan District of Shenzhen, No. 2015052~~
文摘Objective： To observe the clinical effect of liver-regulating and blood-tonifying acupuncture in the treatment of cervical spondylosis of vertebral artery type. Methods： A total of 60 cases meeting the inclusion criteria were randomized into an observation group and a control group, 30 cases in each group. Cases in the observation group received liver-regulating and blood-tonifying acupuncture, in which Jiaji （EX-B 2） points of C3_C7, Ganshu （BL 18） and Geshu （BL 17） were used; cases in the control group received routine acupuncture treatment in which jiaji （EX-B2） points of C3.C7 were used. Both groups received 5 times of treatment in a week with a 2-day rest, symptoms evaluation was done after 4 weeks of treatment, and then therapeutical effect was evaluated. Results： The total effective rate was 90.0% in the observation group, 73.3% in the control group, and inter-group comparison showed a statistical significance （P〈0.05）. After treatment, the symptoms and signs scores in both groups all substantially dropped, showing statistical significances （both P〈0.05）; inter-group comparison showed that the improvements in subscales of dizziness, pressing pain along vertebrae and revolve-cervix test in the observation group were superior than those in the control group, showing statistical significances （all P〈0.05）, while the inter-group comparisons of subscales of headache and vomiting and nause didn＇t show any statistical significances after treatment （both P〉0.05）. Conclusion： Liver-regulating and blood-tonifying acupuncture is effective in treating cervical spondylosis of vertebral artery type, and it can significantly improve patients＇ symptoms and signs.
基金supported by the Open Fund for Colleges and Universities Innovation Platform of Hunan Province,No.14K070Key Project of Hunan Province Administration of Traditional Chinese Medicine,No.201310Innovation Fund Project for Graduate Student of Hunan Province,No.CX2014B360~~
文摘Objective： To observe the effect of electroacupuncture （EA） on the expression of erythropoie-tin-producing hepatocyte receptor B2 （EphB2） in the cortex around the infracted area of middle cerebral artery occlusion （MCAO） rats at different timing, and to reveal the possible mechanism of acupuncture in the treatment of cerebral ischemia. Methods： A total of 180 male Sprague-Dawley （SD） rats were randomly divided into a sham operation group, a model group, an acupoint group and a non-acupoint group, with 45 rats in each group. Rats in each group were further divided into three subgroups： postoperative 3 d, postoperative 14 d and postoperative 2l d groups, with 15 rats in each subgroup. The MCAO model was made by the modified occlusion method. The neurological function score, 2,3,5-triphenyl tetrazolium chloride （TFC） staining, immunohistochemistry assay, immunofluorescence double labeling method and Western blot were used to detect the corresponding indicators. Results： The neurological impairment of rats was most obvious at postoperative 3 d, and then gradually improved with time, which was more significant in the acupoint group （P〈0.05）. The change of infarcted volume was consistent with the neurological function impairment. The number of EphB2 positive cells （EphB2＋） around the infarcted area was decreased significantly at postoperative 3 d, and then gradually improved with time, which returned to the same level as that in the sham operation group at postoperative 21 d. The increase was most significant in the acupoint group （P〈0.05）, and the positive cell number was higher than that in the sham operation group （P〈0.01）. Western blot and immunohistochemistry results were basically consistent. Immunofluorescence displayed that EphB2＋ and postsynaptic density-95 positive （PSD-95＋） were co-expressed, after the MCAO operation, in the cortical neuron around the infracted area, and the number of co-expressing cells was increased gradually with time, which was most significant in the acupoint group （P〈0.05）. Conclusion： Electroacupuncture at Ganshu （BL 18） and Shenshu （BL 23） can significantly improve the neurological function and cerebral infarcted volume ratio of MCAO rats, which may be related to the activation of EphB2 expression in cortex around the infracted area and the promotion of synaptic remodeling.
基金supported by Open Fund for Colleges and Universities Innovation Platform of Hunan Province, No.14K070Key Project of Hunan Province Administration of Traditional Chinese Medicine, No. 201310~~
文摘Objective： To investigate the effect of electroacupuncture （EA） at Ganshu （BL 18） and Shenshu （BL 23） on vascular endothelial growth factor （VEGF） and platelet endothelial cell adhesion molecule-1 （PECAM-1）/CD31 around the cerebral infarction focus in middle cerebral artery occlusion （MCAO） rats and the possible mechanism, thus to provide a new strategy for the treatment of cerebral ischemic stroke by acupuncture. Methods： A total of 180 healthy male Sprague-Dawley （SD） rats were randomly divided into a sham operation group, a model group, an acupoint group and a non-acupoint group, 45 rats in each group. MCAO model was established using the modified line-embolus method in all rats except for those in the sham operation group; rats in the acupoint group were treated with EA at Ganshu （BL 18） and Shenshu （BL 23）; rats in the non-acupoint group were treated with EA at the control points; rats in other 2 groups were only subjected to bundling without treatment. Ten rats in each group were randomly selected on the 3rd day, the 14th day and the 21st day after acupuncture stimulation to test the neurological function impairment. The expression levels of CD31 and VEGF were also detected. Results： Compared with the model group and non-acupoint group, the neurological function score of the acupoint group was decreased at each time point, and the differences were statistically significant （P〈0.05, P〈0.01）. The expressions of VEGF and CD31 in each group were the lowest on the 3rd day, reached the peak on the 14th day and still remained at high level on the 21st day. And the differences among groups were statistically significant both on the 14th day and the 21st day （P〈0.05, P〈0.01）. Compared with the model group and the non-acupoint group, the expressions of VEGF and CD31 in the acupoint group were increased, and the differences were statistically significant （all P〈0.05）. Conclusion： EA at Ganshu （BL 18） and Shenshu （BL 23） can significantly improve the neurological function score of MCAO model rats, and shows protective effect on cerebral ischemia. The protective mechanism may be related to the up-regulation of CD31 and VEGF expression around the cerebral infarction focus in the MCAO model rats and induction of angiogenesis.
文摘Objective:To observe the clinical efficacy of acupoint injection with salmon calcitonin for back pain in elderly patients with primary osteoporosis.Methods:A total of 76 patients were selected and randomly divided into two groups by the random number table method,with 39 cases in the treatment group and 37 cases in the control group,respectively.Patients in both groups received routine anti-osteoporosis treatment.Patients in the treatment group received additional acupoint injection with salmon calcitonin at bilateral Pishu(BL 20)and Shenshu(BL 23),while patients in the control group received additional intramuscular injection with salmon calcitonin.The treatments for both groups were given once a day and lasted for 4 weeks.Visual analog scale(VAS)and Chinese Oswestry disability index(CODI)scores were observed before treatment,after 2 weeks and 4 weeks of treatment,and the use of analgesics during the treatment were recorded.Results:After treatment,the clinical efficacy in the treatment group was more significant than that in the control group(P<0.05).After 2 weeks and 4 weeks of treatment,the VAS scores in both groups showed significant intra-group and between-group differences(all P<0.05),and the CODI scores in both groups showed significant intra-group differences(all P<0.05).After 2 weeks of treatment,the CODI score showed no significant between-group difference(P>0.05).After 4 weeks of treatment,the improvement of CODI score in the treatment group was more significant than that in the control group(P<0.05).During the treatment,2 cases in the treatment group took analgesics versus 8 cases in the control group,and the result showed a significant between-group difference(P<0.05).Conclusion:For back pain in elderly patients with primary osteoporosis,based on the routine treatment of oral medication,the clinical efficacy of acupoint injection with salmon calcitonin at bilateral Pishu(BL 20)and Shenshu(BL 23)is more significant than that of intramuscular injection.Acupoint injection treatment can improve patients'conditions and reduce the use of analgesics.
