在双智城市建设和城市道路交通秩序管理科学化、精细化、智能化水平背景下,探索属地院校和政府部门共建智慧交通信号配时中心。通过应用高新技术和智能化手段,优化城市交通信号系统的配时策略,在促进双智城市建设、减少交通拥堵、提高...在双智城市建设和城市道路交通秩序管理科学化、精细化、智能化水平背景下,探索属地院校和政府部门共建智慧交通信号配时中心。通过应用高新技术和智能化手段,优化城市交通信号系统的配时策略,在促进双智城市建设、减少交通拥堵、提高城市整体交通运行能效方面有着非常重要的作用。In the context of the construction of dual smart cities and the scientific, refined, and intelligent management of urban road traffic order, the paper explores the joint construction of smart traffic signal timing centers by local universities and government departments, optimizes the timing strategy of urban traffic signal systems through the application of high-tech and intelligent means, which play a very important role in promoting the construction of dual smart cities, reducing traffic congestion, and improving the overall energy efficiency of urban traffic operation.展开更多
Pericentrin, a conserved centrosomal component, provides the structural scaffold to anchor numerous centrosomal proteins, and thus plays an essential role in the organization and function of the centrosome and the mit...Pericentrin, a conserved centrosomal component, provides the structural scaffold to anchor numerous centrosomal proteins, and thus plays an essential role in the organization and function of the centrosome and the mitotic spindle. Although pericentrin was shown to localize in the cytoplasm and reported to be sensitive to leptomycin B （LMB）, a specific inhibitor of Crml, the regions within pericentrin that serve as signals for transporting in and out of the nucleus have not yet been identified. In this study, we identified five novel nuclear export signals （NESs） in pericentrin with diverse export activities. All of the five NESs could bind to Crml in a LMB-sensitive way when mediating the nuclear export of pericentrin. We also demonstrated that the region of amino acids 8-42 in pericentrin contains a tripartite nuclear localization signal （NLS） consisting of three clusters of basic amino acids. The NLS of pericentrin binds to importin β directly or via the adaptor importin α to form the import complex, which could be disrupted by RanQ69L, a dominant-negative Ran GTPase possessing high affinity for importin β. Furthermore, we found that mutation of the NESs in full-length pericentrin results in both nuclear and cytoplasmic localization, and mutation of the NLS abolishes the nuclear import of pericentrin. On the basis of these results, we suggest that the NESs and NLS of pericentrin are essential for its subcellular localization and nucleocytoplasmic trafficking during the cell cycle.展开更多
At the CERN large hadron collider （LHC）, production of the Higgs boson in association with Z or W bosons provides a dramatic experimental signal for detecting the standard model （SM） Higgs boson. In this paper, we...At the CERN large hadron collider （LHC）, production of the Higgs boson in association with Z or W bosons provides a dramatic experimental signal for detecting the standard model （SM） Higgs boson. In this paper, we consider the contributions of the left-right twin Higgs （LRTH） model to the processes qq＇ --~ Z（W）H. Our numerical results show that, in the favorable parameter spaces, the cross sections deviate distinctly from the predictions of the SM. The possible signals of the LRTH model can be detected via these processes at the LHC experiments.展开更多
Innate immunity offers the first line of defense against infections and other types of danger such as tumorigenesis. Its discovery provides tremendous therapeutic opportunities for numerous human diseases. Delving int...Innate immunity offers the first line of defense against infections and other types of danger such as tumorigenesis. Its discovery provides tremendous therapeutic opportunities for numerous human diseases. Delving into the structural basis of signal transduction by innate immune receptors, our lab has recently helped to establish the new paradigm in which innate immune receptors transduce ligand-binding signals through formation of higher-order assemblies containing intracellular adapters, signaling enzymes and their substrates. These large signalosome assemblies may be visible under light microscopy as punctate structures in the μm scale, connecting to the underlying molecular structures in the nm scale. They drive proximity-induced enzyme activation, and provide a mechanism for signaling amplification by nucleated polymerization. These supramolecular signaling complexes also open new questions on their cellular organization and mode of regulation, pose challenges to our methodology, and afford valuable implications in drug discovery against these medically important pathways.展开更多
Amplifier is at the heart of experiments carrying out the precise measurement of a weak signal. An idea quantum amplifier should have a large gain and minimum added noise simultaneously. Here, we consider the quantum ...Amplifier is at the heart of experiments carrying out the precise measurement of a weak signal. An idea quantum amplifier should have a large gain and minimum added noise simultaneously. Here, we consider the quantum measurement properties of the cavity with the OPA medium in the op-amp mode to amplify an input signal. We show that our nonlinear-cavity quantum amplifier has large gain in the single-value stable regime and achieves quantum limit unconditionally.展开更多
文摘在双智城市建设和城市道路交通秩序管理科学化、精细化、智能化水平背景下,探索属地院校和政府部门共建智慧交通信号配时中心。通过应用高新技术和智能化手段,优化城市交通信号系统的配时策略,在促进双智城市建设、减少交通拥堵、提高城市整体交通运行能效方面有着非常重要的作用。In the context of the construction of dual smart cities and the scientific, refined, and intelligent management of urban road traffic order, the paper explores the joint construction of smart traffic signal timing centers by local universities and government departments, optimizes the timing strategy of urban traffic signal systems through the application of high-tech and intelligent means, which play a very important role in promoting the construction of dual smart cities, reducing traffic congestion, and improving the overall energy efficiency of urban traffic operation.
文摘Pericentrin, a conserved centrosomal component, provides the structural scaffold to anchor numerous centrosomal proteins, and thus plays an essential role in the organization and function of the centrosome and the mitotic spindle. Although pericentrin was shown to localize in the cytoplasm and reported to be sensitive to leptomycin B （LMB）, a specific inhibitor of Crml, the regions within pericentrin that serve as signals for transporting in and out of the nucleus have not yet been identified. In this study, we identified five novel nuclear export signals （NESs） in pericentrin with diverse export activities. All of the five NESs could bind to Crml in a LMB-sensitive way when mediating the nuclear export of pericentrin. We also demonstrated that the region of amino acids 8-42 in pericentrin contains a tripartite nuclear localization signal （NLS） consisting of three clusters of basic amino acids. The NLS of pericentrin binds to importin β directly or via the adaptor importin α to form the import complex, which could be disrupted by RanQ69L, a dominant-negative Ran GTPase possessing high affinity for importin β. Furthermore, we found that mutation of the NESs in full-length pericentrin results in both nuclear and cytoplasmic localization, and mutation of the NLS abolishes the nuclear import of pericentrin. On the basis of these results, we suggest that the NESs and NLS of pericentrin are essential for its subcellular localization and nucleocytoplasmic trafficking during the cell cycle.
基金Supported by the National Natural Science Foundation of China under Grant Nos. 10975047 and 10979008
文摘At the CERN large hadron collider （LHC）, production of the Higgs boson in association with Z or W bosons provides a dramatic experimental signal for detecting the standard model （SM） Higgs boson. In this paper, we consider the contributions of the left-right twin Higgs （LRTH） model to the processes qq＇ --~ Z（W）H. Our numerical results show that, in the favorable parameter spaces, the cross sections deviate distinctly from the predictions of the SM. The possible signals of the LRTH model can be detected via these processes at the LHC experiments.
文摘Innate immunity offers the first line of defense against infections and other types of danger such as tumorigenesis. Its discovery provides tremendous therapeutic opportunities for numerous human diseases. Delving into the structural basis of signal transduction by innate immune receptors, our lab has recently helped to establish the new paradigm in which innate immune receptors transduce ligand-binding signals through formation of higher-order assemblies containing intracellular adapters, signaling enzymes and their substrates. These large signalosome assemblies may be visible under light microscopy as punctate structures in the μm scale, connecting to the underlying molecular structures in the nm scale. They drive proximity-induced enzyme activation, and provide a mechanism for signaling amplification by nucleated polymerization. These supramolecular signaling complexes also open new questions on their cellular organization and mode of regulation, pose challenges to our methodology, and afford valuable implications in drug discovery against these medically important pathways.
基金Supported by the National Natural Science Foundation of China under Grant Nos.11365006,11364006the Natural Science Foundation of Guizhou Province QKHLHZ[2015]7767
文摘Amplifier is at the heart of experiments carrying out the precise measurement of a weak signal. An idea quantum amplifier should have a large gain and minimum added noise simultaneously. Here, we consider the quantum measurement properties of the cavity with the OPA medium in the op-amp mode to amplify an input signal. We show that our nonlinear-cavity quantum amplifier has large gain in the single-value stable regime and achieves quantum limit unconditionally.
