We have developed a cell-free system that can trigger the nuclei purified from mouse liver and suspensioncultured carrot cells to undergo apoptosis as defined by the formation of apoptotic bodies and nucleosomal DNA f...We have developed a cell-free system that can trigger the nuclei purified from mouse liver and suspensioncultured carrot cells to undergo apoptosis as defined by the formation of apoptotic bodies and nucleosomal DNA fragments. The effects of different divalent cations and cycloheximide on DNA cleavage in this system were assessed.The fact that nuclei of plant cells can be induced to undergo apoptosis in a cell-free animal system suggests that animals and plants share a common signal transduction pathway triggering in the initiation stage of apoptosis.展开更多
An embedded underground coal seam carries channel waves of low seismic velocity along a stratigraphic rock-coal-rock sequence.In a homogeneous and isotropic seam, seismic waves propagate as trapped waves within the se...An embedded underground coal seam carries channel waves of low seismic velocity along a stratigraphic rock-coal-rock sequence.In a homogeneous and isotropic seam, seismic waves propagate as trapped waves within the seam, which leads to propagation of channel waves.We describe how to set up a field test for transmission in order to acquire channel waves in a coal seam.Because channel wave signals are non-stationary in their frequencies and amplitudes, a necessary velocity spectrum and wavelet transformation analysis are applied to interpret the characteristics of channel waves.The advantage of using a wavelet transformation is that different resolutions can be obtained at different times and different frequencies.According to analysis of the seismic signals acquired in the S7 sensor hole, it was clearly shown that the characteristics of channel waves are lower frequencies and attenuation which can guide an effective wave for detecting voids, boundaries and faults in coal seams with strong roofs and floors.展开更多
Based on advantages of technology of distributive fiber-optic temperature sensing and specific to its applications in monitoring mine conflagration, the corresponding Processes such as connection arrangement, signal t...Based on advantages of technology of distributive fiber-optic temperature sensing and specific to its applications in monitoring mine conflagration, the corresponding Processes such as connection arrangement, signal transmission and monitoring were illustrated. As applied in Sitai Coal Mine of Datong Coal Mine Group Co., this method is effective and accurate and could provide reliable gist for monitoring spontaneous combustion in gob area of mines.展开更多
As a pivotal signal pathway,the Ras/Raf/MEK/ERK cascade can be activated by multiple extracellular stimuli and can transmit signals to diverse substrates.It remains to be elucidated how so many different signals can b...As a pivotal signal pathway,the Ras/Raf/MEK/ERK cascade can be activated by multiple extracellular stimuli and can transmit signals to diverse substrates.It remains to be elucidated how so many different signals can be variously transferred by only two MEK molecules(MEK1 and MEK2) .Because of technological limitations the complete structures of the MEKs are still unavailable.Here,we report the full-length structure of MEK2 obtained by homology modeling and molecular dynamics simulations.The simulations show that the N-terminal part of MEK2 is highly flexible and this flexibility may enable MEK2 to interact with ERKs and other ligands in diverse manners that correspond to various upstream signals and downstream consequences.展开更多
Feedback plays an important role in various biological signal transmission systems. In this paper, a signaling cascade system(including three layers: input(S), intermediate(V), output(X) components) is employed to stu...Feedback plays an important role in various biological signal transmission systems. In this paper, a signaling cascade system(including three layers: input(S), intermediate(V), output(X) components) is employed to study the fluctuations and net synergy in information transmission, in which the V component is regulated by itself or the X component, and each feedback on V is either positive or negative. The Fano factor, the net synergy, and the signalto-noise ratio(SNR) of signaling cascade with the four possible feedback types are theoretically derived by using linear noise approximation of the master equation, and the ability of information transmission through the signaling cascade is characterized by using the partial information decomposition of information theory. It is found that the signaling cascade exhibits different responses to the four feedback mechanisms, which depend on the relationships between degradation rates of components. Our results not only clarify the dependence of the Fano factor, net synergy, and SNR on the feedback regulations with the varying of degradation rates of components, but also imply that living cells could utilize different feedback mechanisms to adapt to the external fluctuating environments.展开更多
文摘We have developed a cell-free system that can trigger the nuclei purified from mouse liver and suspensioncultured carrot cells to undergo apoptosis as defined by the formation of apoptotic bodies and nucleosomal DNA fragments. The effects of different divalent cations and cycloheximide on DNA cleavage in this system were assessed.The fact that nuclei of plant cells can be induced to undergo apoptosis in a cell-free animal system suggests that animals and plants share a common signal transduction pathway triggering in the initiation stage of apoptosis.
基金Project B2532532 supported by the U.S. Mine Safety and Health Administration
文摘An embedded underground coal seam carries channel waves of low seismic velocity along a stratigraphic rock-coal-rock sequence.In a homogeneous and isotropic seam, seismic waves propagate as trapped waves within the seam, which leads to propagation of channel waves.We describe how to set up a field test for transmission in order to acquire channel waves in a coal seam.Because channel wave signals are non-stationary in their frequencies and amplitudes, a necessary velocity spectrum and wavelet transformation analysis are applied to interpret the characteristics of channel waves.The advantage of using a wavelet transformation is that different resolutions can be obtained at different times and different frequencies.According to analysis of the seismic signals acquired in the S7 sensor hole, it was clearly shown that the characteristics of channel waves are lower frequencies and attenuation which can guide an effective wave for detecting voids, boundaries and faults in coal seams with strong roofs and floors.
基金Supported by the National Natural Science Foundation of China （50375026,50375028）
文摘Based on advantages of technology of distributive fiber-optic temperature sensing and specific to its applications in monitoring mine conflagration, the corresponding Processes such as connection arrangement, signal transmission and monitoring were illustrated. As applied in Sitai Coal Mine of Datong Coal Mine Group Co., this method is effective and accurate and could provide reliable gist for monitoring spontaneous combustion in gob area of mines.
基金supported by the National Natural Science Foundation of China(Grant No.30670791)the National Basic Research Program of China(Grant No.2009CB918301)+1 种基金the National High Technology Research and Development Program of China(Grant No.2008AA02Z124)the State Key Laboratory of Proteomics(Grant No.SKLP-Y200810)
文摘As a pivotal signal pathway,the Ras/Raf/MEK/ERK cascade can be activated by multiple extracellular stimuli and can transmit signals to diverse substrates.It remains to be elucidated how so many different signals can be variously transferred by only two MEK molecules(MEK1 and MEK2) .Because of technological limitations the complete structures of the MEKs are still unavailable.Here,we report the full-length structure of MEK2 obtained by homology modeling and molecular dynamics simulations.The simulations show that the N-terminal part of MEK2 is highly flexible and this flexibility may enable MEK2 to interact with ERKs and other ligands in diverse manners that correspond to various upstream signals and downstream consequences.
基金Supported by the National Natural Science Foundation of China under Grant Nos.11775091 and 11474117
文摘Feedback plays an important role in various biological signal transmission systems. In this paper, a signaling cascade system(including three layers: input(S), intermediate(V), output(X) components) is employed to study the fluctuations and net synergy in information transmission, in which the V component is regulated by itself or the X component, and each feedback on V is either positive or negative. The Fano factor, the net synergy, and the signalto-noise ratio(SNR) of signaling cascade with the four possible feedback types are theoretically derived by using linear noise approximation of the master equation, and the ability of information transmission through the signaling cascade is characterized by using the partial information decomposition of information theory. It is found that the signaling cascade exhibits different responses to the four feedback mechanisms, which depend on the relationships between degradation rates of components. Our results not only clarify the dependence of the Fano factor, net synergy, and SNR on the feedback regulations with the varying of degradation rates of components, but also imply that living cells could utilize different feedback mechanisms to adapt to the external fluctuating environments.
