即时检验（point of care testing,POCT）是当今临床检验发展的趋势之一。侧流免疫法,最早被称为“溶胶粒子免疫法”,它是以含有被粒子标记的待测抗原（抗体）或被粒子标记的免疫复合物[待测抗原（抗体）]与被粒子标记的抗体（抗原）生...即时检验（point of care testing,POCT）是当今临床检验发展的趋势之一。侧流免疫法,最早被称为“溶胶粒子免疫法”,它是以含有被粒子标记的待测抗原（抗体）或被粒子标记的免疫复合物[待测抗原（抗体）]与被粒子标记的抗体（抗原）生成的免疫复合物]的液体样品或提取溶液为流动相,以固定有检测线和控制线的条状聚合物层析材料为固定相,流动相通过毛细作用在固定相中向前移动的过程中。展开更多
Shear probe works under a tough environment where the turbulence signals to be measured are very weak. The measured turbulence signals often contain a large amount of noise. Due to wide frequency band, noise signals c...Shear probe works under a tough environment where the turbulence signals to be measured are very weak. The measured turbulence signals often contain a large amount of noise. Due to wide frequency band, noise signals cannot be effectively removed by traditional methods based on Fourier transform. In this paper, a wavelet thresholding denoising method is proposed for turbulence signal processing in that wavelet analysis can be used for multi-resolution analysis and can extract local characteristics of the signals in both time and frequency domains. Turbulence signal denoising process is modeled based on the wavelet theory and characteristics of the turbulence signal. The threshold and decomposition level, as well as the procedure of the turbulence signal denoising, are determined using the wavelet thresholding method. The proposed wavelet thresholding method was validated by turbulence signal denoising of the Western Pacific Ocean trial data. The results show that the propsed method can reduce the noise in the measured signals by shear probes, and the frequency spectrums of the denoised signal correspond well to the Nasmyth spectrum.展开更多
This paper investigates the effect of carried-envelope phase on transient process in a cascade-type atomic system, which is driven by two ultrashort laser pulses （probe and signal laser）. It is found that the one- a...This paper investigates the effect of carried-envelope phase on transient process in a cascade-type atomic system, which is driven by two ultrashort laser pulses （probe and signal laser）. It is found that the one- and two-photon processes corresponding to pathway ｜0〉→｜1〉and ｜0〉→｜1〉→｜2〉 can be enhanced or ,suppressed by modulating the carried-envelope phases of probe laser pulse. Our numerical results also show that the transient populations of two excited states can be periodically affected by the carried-envelope phase of probe laser pulse. With certain time, the partial population transfer between two exited states can be realized just by adjusting the carried-envelope phase of probe laser pulse.展开更多
Monitoring telomerase activity with high sensitive and reliable is of great importance to cancer analysis. In this paper, we report a sensitive and facile method to detect telomerase activity using AIEgens mod- ified ...Monitoring telomerase activity with high sensitive and reliable is of great importance to cancer analysis. In this paper, we report a sensitive and facile method to detect telomerase activity using AIEgens mod- ified probe （TPE-Py-DNA） as a fluorescence reporter and exonuclease llI （Exo lIl） as a signal amplifier. With the aid of telomerase, repeat units （TrAGGG）n are extended from the end of template substrate oligonucleotides （TS primer） that form duplex DNAs with TPE-Py-DNA. Then, Exo llI catalyzes the diges- tion of duplex DNAs, liberating elongation product and releasing hydrophobic TPE-Py. The released hydrophobic TPE-Py aggregate together and produce a telomerase-activity-related fluorescence signal. The liberated product hybridizes with another TPE-Py-DNA probe, starting the second cycle. Finally, we obtain the target-to-signal amplification ratio of 1 ：N2. This strategy exhibits good performance for detecting clinical urine samples （distinguishing 15 cancer patients＇ samples from 8 healthy ones） and checking intracellular telomerase activity （differentiating cell lines including HeLa, MDA-MB-231, MCF-7, A375, HLF and MRC-5 from the cells pretreated with telomerase-related drug）, which shows its potential in clinical diagnosis as well as therapeutic monitoring of cancer.展开更多
cAMP is an important second messenger that is capable of controlling a wide array of cellular processes, including glycogen, sugar and lipid metabolism. Here we report the design and construction of a novel geneticall...cAMP is an important second messenger that is capable of controlling a wide array of cellular processes, including glycogen, sugar and lipid metabolism. Here we report the design and construction of a novel genetically encoded fluorescent sensor for cAMP. The sensor was realized by fusing E. coli CAP protein with cpYFP, and displayed a one-fold fluorescence change towards c AMP binding. Further characterization assays demonstrated that the sensor had high affinity for cAMP and fast response kinetics.The development of our sensor could be a useful supplement to existing methods for cAMP detection.展开更多
文摘即时检验（point of care testing,POCT）是当今临床检验发展的趋势之一。侧流免疫法,最早被称为“溶胶粒子免疫法”,它是以含有被粒子标记的待测抗原（抗体）或被粒子标记的免疫复合物[待测抗原（抗体）]与被粒子标记的抗体（抗原）生成的免疫复合物]的液体样品或提取溶液为流动相,以固定有检测线和控制线的条状聚合物层析材料为固定相,流动相通过毛细作用在固定相中向前移动的过程中。
基金Supported by National Natural Science Foundation of China (No. 50835006 and No. 51005161)National High-Tech R&D Program ("863"Program) of China (No. 2010AA09Z102)
文摘Shear probe works under a tough environment where the turbulence signals to be measured are very weak. The measured turbulence signals often contain a large amount of noise. Due to wide frequency band, noise signals cannot be effectively removed by traditional methods based on Fourier transform. In this paper, a wavelet thresholding denoising method is proposed for turbulence signal processing in that wavelet analysis can be used for multi-resolution analysis and can extract local characteristics of the signals in both time and frequency domains. Turbulence signal denoising process is modeled based on the wavelet theory and characteristics of the turbulence signal. The threshold and decomposition level, as well as the procedure of the turbulence signal denoising, are determined using the wavelet thresholding method. The proposed wavelet thresholding method was validated by turbulence signal denoising of the Western Pacific Ocean trial data. The results show that the propsed method can reduce the noise in the measured signals by shear probes, and the frequency spectrums of the denoised signal correspond well to the Nasmyth spectrum.
文摘This paper investigates the effect of carried-envelope phase on transient process in a cascade-type atomic system, which is driven by two ultrashort laser pulses （probe and signal laser）. It is found that the one- and two-photon processes corresponding to pathway ｜0〉→｜1〉and ｜0〉→｜1〉→｜2〉 can be enhanced or ,suppressed by modulating the carried-envelope phases of probe laser pulse. Our numerical results also show that the transient populations of two excited states can be periodically affected by the carried-envelope phase of probe laser pulse. With certain time, the partial population transfer between two exited states can be realized just by adjusting the carried-envelope phase of probe laser pulse.
基金supported by the National Natural Science Foundation of China(21375042,21405054,21525523,21574048,and21404028)the National Basic Research Program of China(2015CB932600,2013CB933000,and 2016YFF0100800)+1 种基金the Special Fund for Strategic New Industry Development of Shenzhen,China(JCYJ20150616144425376)1000 Young Talent Program(to F.Xia)
文摘Monitoring telomerase activity with high sensitive and reliable is of great importance to cancer analysis. In this paper, we report a sensitive and facile method to detect telomerase activity using AIEgens mod- ified probe （TPE-Py-DNA） as a fluorescence reporter and exonuclease llI （Exo lIl） as a signal amplifier. With the aid of telomerase, repeat units （TrAGGG）n are extended from the end of template substrate oligonucleotides （TS primer） that form duplex DNAs with TPE-Py-DNA. Then, Exo llI catalyzes the diges- tion of duplex DNAs, liberating elongation product and releasing hydrophobic TPE-Py. The released hydrophobic TPE-Py aggregate together and produce a telomerase-activity-related fluorescence signal. The liberated product hybridizes with another TPE-Py-DNA probe, starting the second cycle. Finally, we obtain the target-to-signal amplification ratio of 1 ：N2. This strategy exhibits good performance for detecting clinical urine samples （distinguishing 15 cancer patients＇ samples from 8 healthy ones） and checking intracellular telomerase activity （differentiating cell lines including HeLa, MDA-MB-231, MCF-7, A375, HLF and MRC-5 from the cells pretreated with telomerase-related drug）, which shows its potential in clinical diagnosis as well as therapeutic monitoring of cancer.
基金National Basic Research Foundation of China(Grant No.2017YFA0505202)the National Natural Science Foundation of China(Grant No.91853107)
文摘cAMP is an important second messenger that is capable of controlling a wide array of cellular processes, including glycogen, sugar and lipid metabolism. Here we report the design and construction of a novel genetically encoded fluorescent sensor for cAMP. The sensor was realized by fusing E. coli CAP protein with cpYFP, and displayed a one-fold fluorescence change towards c AMP binding. Further characterization assays demonstrated that the sensor had high affinity for cAMP and fast response kinetics.The development of our sensor could be a useful supplement to existing methods for cAMP detection.
