This paper describes the effect of Frat-1, Idax and Axam toward the Wnt signaling pathway. The Wnt pathway is an important intracellular signaling pathway that plays a crucial role in the cell cycle process at several...This paper describes the effect of Frat-1, Idax and Axam toward the Wnt signaling pathway. The Wnt pathway is an important intracellular signaling pathway that plays a crucial role in the cell cycle process at several levels of development. Understanding this pathway thoroughly will enable identification of new drug targets and thereby open avenues to more effective therapies for a range of Wnt pathway related diseases such as cancer, osteoporosis, and mesothelioma. Dishevelled （Dsh） is one of the multi-module proteins working m the Wnt pathway. The function of Dsh depends on the regulators which bind to it, components it interacts with and most importantly the Wnt signal. It is important to note that positive regulators are those factors that induce gene transcription. Conversely, the negative regulators induce β-Catenin degradation. The positive regulator such as Frat- 1 would promote the Wnt signaling pathway and downstream gene transcription. Meanwhile the negative regulator such as Idax and Axam would inhibit the signaling pathway and promotes β-Catenin degradation which will thereby prevent aberrant gene transcription.展开更多
the power network fault detection system can only analyze all kinds of fault signal in stationary sequence： the internal grid and external disturbance presents degeneration, tiny, signal intensity changes randomly fl...the power network fault detection system can only analyze all kinds of fault signal in stationary sequence： the internal grid and external disturbance presents degeneration, tiny, signal intensity changes randomly fluctuate, that will cause the system to detect the fault isolation ability of confusion and small fault signal is not strong; The article propose a method of power network fault detection for based on GSM, Through the underlying sensing equipment acquisition abnormal information of current, voltage power in the network and GSM networking scheme can filter the interference factors in extraction of fault information from and attribute value. The embedded gateway take STM32 chip as the core to monitoring data processing, to achieve a unified data management and user remote access, realizing method of system software are given, to construct the monitor management information platform. The actual test system show that, identification diagnosis ability of fault signal separation ability and small signal increases 17%, also meet the requirements.展开更多
This paper presents a novel strategy for the response enhancement of olfactory sensory neurons (OSNs)-based biosensors by monitoring the enhancive responses of OSNs to odorants. An OSNs-based biosensor was developed o...This paper presents a novel strategy for the response enhancement of olfactory sensory neurons (OSNs)-based biosensors by monitoring the enhancive responses of OSNs to odorants. An OSNs-based biosensor was developed on the basis of the light addressable potentiometric sensor (LAPS), in which rat OSNs were cultured on the surface of LAPS chip and served as sensing elements. LY294002, the specific inhibitor of phosphatidylinositol 3-kinase (PI3K), was used to enhance the responses of OSNs to odorants. The responses of OSNs to odorants with and without the treatment of LY294002 were recorded by LAPS. The results show that the enhancive effect of LY294002 was recorded efficiently by LAPS and the responses of this OSNs-LAPS hybrid biosensor were enhanced by LY294002 by about 1.5-fold. We conclude that this method can enhance the responses of OSNs-LAPS hybrid biosensors, which may provide a novel strategy for the bioelectrical signal monitor of OSNs in biosensors. It is also suggested that this strategy may be applicable to other kinds of OSNs-based biosensors for cellular activity detection, such as microelectrode array (MEA) and field effect transistor (FET).展开更多
MicroRNA （miRNA） alterations in response to changes in an extracellular microenvironment have been observed and consid- ered as one of the major mechanisms for epigenetic modifications of the cell. While enormous ef...MicroRNA （miRNA） alterations in response to changes in an extracellular microenvironment have been observed and consid- ered as one of the major mechanisms for epigenetic modifications of the cell. While enormous efforts have been made in the understanding of the role of miRNAs in regulating cellular responses to the microenvironment, the mechanistic insight into how extracellular signals can be transduced into miRNA alterations in cells is still lacking. Interestingly, recent studies have shown that ion channels/transporters, which are known to conduct or transport ions across the cell membrane, also exhibit changes in levels of expression and activities in response to changes of extraceUular microenvironment. More importantly, al- terations in expression and function of ion channels/transporters have been shown to result in changes in miRNAs that are known to change in response to alteration of the microenvironment. In this review, we aim to summarize the recent data demonstrating the ability of ion channels/transporters to transduce extracellular signals into miRNA changes and propose a potential link between cells and their microenvironment through ion channels/transporters. At the same time, we hope to pro- vide new insights into epigenetic regulatory mechanisms underlying a number of physiological and pathological processes, in- eluding embryo development and cancer metastasis.展开更多
文摘This paper describes the effect of Frat-1, Idax and Axam toward the Wnt signaling pathway. The Wnt pathway is an important intracellular signaling pathway that plays a crucial role in the cell cycle process at several levels of development. Understanding this pathway thoroughly will enable identification of new drug targets and thereby open avenues to more effective therapies for a range of Wnt pathway related diseases such as cancer, osteoporosis, and mesothelioma. Dishevelled （Dsh） is one of the multi-module proteins working m the Wnt pathway. The function of Dsh depends on the regulators which bind to it, components it interacts with and most importantly the Wnt signal. It is important to note that positive regulators are those factors that induce gene transcription. Conversely, the negative regulators induce β-Catenin degradation. The positive regulator such as Frat- 1 would promote the Wnt signaling pathway and downstream gene transcription. Meanwhile the negative regulator such as Idax and Axam would inhibit the signaling pathway and promotes β-Catenin degradation which will thereby prevent aberrant gene transcription.
文摘the power network fault detection system can only analyze all kinds of fault signal in stationary sequence： the internal grid and external disturbance presents degeneration, tiny, signal intensity changes randomly fluctuate, that will cause the system to detect the fault isolation ability of confusion and small fault signal is not strong; The article propose a method of power network fault detection for based on GSM, Through the underlying sensing equipment acquisition abnormal information of current, voltage power in the network and GSM networking scheme can filter the interference factors in extraction of fault information from and attribute value. The embedded gateway take STM32 chip as the core to monitoring data processing, to achieve a unified data management and user remote access, realizing method of system software are given, to construct the monitor management information platform. The actual test system show that, identification diagnosis ability of fault signal separation ability and small signal increases 17%, also meet the requirements.
基金supported by the National Natural Science Foundation ofChina (No. 60725102)the Natural Science Foundation of Zhejiang Province, China (No. R205505)
文摘This paper presents a novel strategy for the response enhancement of olfactory sensory neurons (OSNs)-based biosensors by monitoring the enhancive responses of OSNs to odorants. An OSNs-based biosensor was developed on the basis of the light addressable potentiometric sensor (LAPS), in which rat OSNs were cultured on the surface of LAPS chip and served as sensing elements. LY294002, the specific inhibitor of phosphatidylinositol 3-kinase (PI3K), was used to enhance the responses of OSNs to odorants. The responses of OSNs to odorants with and without the treatment of LY294002 were recorded by LAPS. The results show that the enhancive effect of LY294002 was recorded efficiently by LAPS and the responses of this OSNs-LAPS hybrid biosensor were enhanced by LY294002 by about 1.5-fold. We conclude that this method can enhance the responses of OSNs-LAPS hybrid biosensors, which may provide a novel strategy for the bioelectrical signal monitor of OSNs in biosensors. It is also suggested that this strategy may be applicable to other kinds of OSNs-based biosensors for cellular activity detection, such as microelectrode array (MEA) and field effect transistor (FET).
基金supported by the Focused Investment Scheme of the Chinese University of Hong KongNational Basic Research Program of China(Grant No. 2012CB944903)+1 种基金GRF-CUHK466111the Fundamental Research Funds for the Central Universities (Jinan University)
文摘MicroRNA （miRNA） alterations in response to changes in an extracellular microenvironment have been observed and consid- ered as one of the major mechanisms for epigenetic modifications of the cell. While enormous efforts have been made in the understanding of the role of miRNAs in regulating cellular responses to the microenvironment, the mechanistic insight into how extracellular signals can be transduced into miRNA alterations in cells is still lacking. Interestingly, recent studies have shown that ion channels/transporters, which are known to conduct or transport ions across the cell membrane, also exhibit changes in levels of expression and activities in response to changes of extraceUular microenvironment. More importantly, al- terations in expression and function of ion channels/transporters have been shown to result in changes in miRNAs that are known to change in response to alteration of the microenvironment. In this review, we aim to summarize the recent data demonstrating the ability of ion channels/transporters to transduce extracellular signals into miRNA changes and propose a potential link between cells and their microenvironment through ion channels/transporters. At the same time, we hope to pro- vide new insights into epigenetic regulatory mechanisms underlying a number of physiological and pathological processes, in- eluding embryo development and cancer metastasis.
