Phytohormone abscisic acid (ABA) was critical for many plant growth and developmental processesincluding seed maturation, germination and response to environmental factors. With the purpose to detectthe possible ABA r...Phytohormone abscisic acid (ABA) was critical for many plant growth and developmental processesincluding seed maturation, germination and response to environmental factors. With the purpose to detectthe possible ABA related signal transduction pathways, we tried to isolate ABA-regulated genes throughcDNA macroarray technology using ABA-treated rice seedling as materials (under treatment for 2, 4, 8 and12 h). Of 6144 cDNA clones tested, 37 differential clones showing induction or suppression for at least onetime, were isolated. Of them 30 and 7 were up- or down-regulated respectively. Sequence analyses revealedthat the putative encoded proteins were involved in different possible processes, including transcription,metabolism and resistance, photosynthesis, signal transduction, and seed maturation. 6 cDNA clones werefound to encode proteins with unknown functions. Regulation by ABA of 7 selected clones relating to signaltransduction or metabolism was confirmed by reverse transcription PCR. In addition, some clones werefurther shown to be regulated by other plant growth regulators including auxin and brassinosteroid, which,however, indicated the complicated interactions of plant hormones. Possible signal transduction pathwaysinvolved in ABA were discussed.展开更多
Image authentication techniques used to protect the recipients against malicious forgery. In this paper, we propose a new image authentication technique based on digital signature. The authentication is verified by co...Image authentication techniques used to protect the recipients against malicious forgery. In this paper, we propose a new image authentication technique based on digital signature. The authentication is verified by comparing the features of the each block in tested image with the corresponding features of the block recorded in the digital signature. The proposed authentication scheme is capable of distinguishing visible but non-malicious changes due to common processing operations from malicious changes. At last our experimental results show that the proposed scheme is not only efficient to protect integrity of image, but also with low computation, which are feasible for practical applications.展开更多
In crab, embryogenesis is a complicated developmental program marked by a series of critical events. RNA-Sequencing technology offers developmental biologists a way to identify many more developmental genes than ever ...In crab, embryogenesis is a complicated developmental program marked by a series of critical events. RNA-Sequencing technology offers developmental biologists a way to identify many more developmental genes than ever before. Here, we present a comprehensive analysis of the transcriptomes of Eriocheir sinensis oosperms （Os） and embryos at the 2-4, cell stage （Cs）, which are separated by a cleavage event. Atotal of 18 923 unigenes were identified, and 403 genes matched with gene ontology （GO） terms related to developmental processes. In total, 432 differentially expressed genes （DEGs） were detected between the two stages. Nine DEGs were specifically expressed at only one stage. These DEGs may be relevant to stage-specific molecular events during development. A number of DEGs related to ＇hedgehog signaling pathway＇, ＇Wnt signaling pathway＇ ＇germplasm＇, ＇nervous system＇, ＇sensory perception＇ and ＇segment polarity＇ were identified as being up-regulated at the Cs stage. The results suggest that these embryonic developmental events begin before the early cleavage event in crabs, and that many of the genes expressed in the two transeriptomes might be maternal genes. Our study provides ample information for further research on the molecular mechanisms underlying crab development.展开更多
In order to improve the effectiveness of traditional time domain identification methods in identifying damping ratios, a new damping ratio identification method based on pattern search is proposed by fluctuating the r...In order to improve the effectiveness of traditional time domain identification methods in identifying damping ratios, a new damping ratio identification method based on pattern search is proposed by fluctuating the reliable natural frequency obtained through traditional time domain identification methods by about 10% to build the boundary conditions, using all the initial identification results to establish the free decay response of the system, and using the pattern search method to correct the initial identification results with the residual sum of squares between the free decay response and the actually measured free-decay signal as the objective function. The proposed method deals with the actually measured free-decay signal with curve fitting and avoids enlarging the identified error caused by intermediate conversion, so it can effectively improve the identified accuracy of damping ratios. Simulations for a room-sized vibration isolation foundation show that the relative errors of analyzed three damping ratios are down to 1.05%, 1.51% and 3.7% by the proposed method from 8.42%, 5.85% and 8.5% by STD method when the noise level is 10%.展开更多
基金Researches were supported by "the State Key Project of Basic Research, G1999011604" "Key Project of Knowledge Innovation, CAS", "the National Natural Science Foundation of China, No.30070073" "National Sciences Foundation of Pan-Deng". We thank Prof.
文摘Phytohormone abscisic acid (ABA) was critical for many plant growth and developmental processesincluding seed maturation, germination and response to environmental factors. With the purpose to detectthe possible ABA related signal transduction pathways, we tried to isolate ABA-regulated genes throughcDNA macroarray technology using ABA-treated rice seedling as materials (under treatment for 2, 4, 8 and12 h). Of 6144 cDNA clones tested, 37 differential clones showing induction or suppression for at least onetime, were isolated. Of them 30 and 7 were up- or down-regulated respectively. Sequence analyses revealedthat the putative encoded proteins were involved in different possible processes, including transcription,metabolism and resistance, photosynthesis, signal transduction, and seed maturation. 6 cDNA clones werefound to encode proteins with unknown functions. Regulation by ABA of 7 selected clones relating to signaltransduction or metabolism was confirmed by reverse transcription PCR. In addition, some clones werefurther shown to be regulated by other plant growth regulators including auxin and brassinosteroid, which,however, indicated the complicated interactions of plant hormones. Possible signal transduction pathwaysinvolved in ABA were discussed.
文摘Image authentication techniques used to protect the recipients against malicious forgery. In this paper, we propose a new image authentication technique based on digital signature. The authentication is verified by comparing the features of the each block in tested image with the corresponding features of the block recorded in the digital signature. The proposed authentication scheme is capable of distinguishing visible but non-malicious changes due to common processing operations from malicious changes. At last our experimental results show that the proposed scheme is not only efficient to protect integrity of image, but also with low computation, which are feasible for practical applications.
基金Supported by the National High Technology Research and Development Program of China(863 Program)(No.2012AA10A409)the Scientific and Technological Innovation Project of Qingdao National Laboratory for Marine Science and Technology(No.2015ASKJ02)the National Natural Science Foundation of China(Nos.41276165 to Dr.CUI Zhaoxia,31302187 to Dr.HUI Min)
文摘In crab, embryogenesis is a complicated developmental program marked by a series of critical events. RNA-Sequencing technology offers developmental biologists a way to identify many more developmental genes than ever before. Here, we present a comprehensive analysis of the transcriptomes of Eriocheir sinensis oosperms （Os） and embryos at the 2-4, cell stage （Cs）, which are separated by a cleavage event. Atotal of 18 923 unigenes were identified, and 403 genes matched with gene ontology （GO） terms related to developmental processes. In total, 432 differentially expressed genes （DEGs） were detected between the two stages. Nine DEGs were specifically expressed at only one stage. These DEGs may be relevant to stage-specific molecular events during development. A number of DEGs related to ＇hedgehog signaling pathway＇, ＇Wnt signaling pathway＇ ＇germplasm＇, ＇nervous system＇, ＇sensory perception＇ and ＇segment polarity＇ were identified as being up-regulated at the Cs stage. The results suggest that these embryonic developmental events begin before the early cleavage event in crabs, and that many of the genes expressed in the two transeriptomes might be maternal genes. Our study provides ample information for further research on the molecular mechanisms underlying crab development.
基金Sponsored by the National Natural Science Foundation of China (Grant No.50675052)
文摘In order to improve the effectiveness of traditional time domain identification methods in identifying damping ratios, a new damping ratio identification method based on pattern search is proposed by fluctuating the reliable natural frequency obtained through traditional time domain identification methods by about 10% to build the boundary conditions, using all the initial identification results to establish the free decay response of the system, and using the pattern search method to correct the initial identification results with the residual sum of squares between the free decay response and the actually measured free-decay signal as the objective function. The proposed method deals with the actually measured free-decay signal with curve fitting and avoids enlarging the identified error caused by intermediate conversion, so it can effectively improve the identified accuracy of damping ratios. Simulations for a room-sized vibration isolation foundation show that the relative errors of analyzed three damping ratios are down to 1.05%, 1.51% and 3.7% by the proposed method from 8.42%, 5.85% and 8.5% by STD method when the noise level is 10%.
