Quantum Fisher information is used to witness the quantum phase transition in a non-Hermitian trapped ion system with balanced gain and loss,from the viewpoint of quantum parameter estimation.We formulate a general no...Quantum Fisher information is used to witness the quantum phase transition in a non-Hermitian trapped ion system with balanced gain and loss,from the viewpoint of quantum parameter estimation.We formulate a general non-unitary dynamic of any two-level non-Hermitian system in the form of state vector.The sudden change in the dynamics of quantum Fisher information occurs at an exceptional point characterizing quantum criticality.The dynamical behaviors of quantum Fisher information are classified into two different ways which depends on whether the system is located in symmetry unbroken or broken phase regimes.In the phase regime where parity and time reversal symmetry are unbroken,the oscillatory evolution of quantum Fisher information is presented,achieving better quantum measurement precision.In the broken phase regime,quantum Fisher information undergoes the monotonically decreasing behavior.The maximum value of quantum estimation precision is obtained at the exceptional point.It is found that the two distinct kinds of behaviors can be verified by quantum entropy and coherence.Utilizing quantum Fisher information to witness phase transition in the non-Hermitian system is emphasized.The results may have potential applications to non-Hermitian quantum information technology.展开更多
Objective Detection and quantification of RNA synthesis in cells is a widely used technique for monitoring cell viability,health,and metabolic rate.After exposure to environmental stimuli,both the internal reference g...Objective Detection and quantification of RNA synthesis in cells is a widely used technique for monitoring cell viability,health,and metabolic rate.After exposure to environmental stimuli,both the internal reference gene and target gene would be degraded.As a result,it is imperative to consider the accurate capture of nascent RNA and the detection of transcriptional levels of RNA following environmental stimulation.This study aims to create a Click Chemistry method that utilizes its property to capture nascent RNA from total RNA that was stimulated by the environment.Methods The new RNA was labeled with 5-ethyluridine(5-EU)instead of uracil,and the azido-biotin medium ligand was connected to the magnetic sphere using a combination of“Click Chemistry”and magnetic bead screening.Then the new RNA was captured and the transcription rate of 16S rRNA was detected by fluorescence molecular beacon(M.B.)and quantitative reverse transcription PCR(qRT-PCR).Results The bacterial nascent RNA captured by“Click Chemistry”screening can be used as a reverse transcription template to form cDNA.Combined with the fluorescent molecular beacon M.B.1,the synthesis rate of rRNA at 37℃is 1.2 times higher than that at 15℃.The 16S rRNA gene and cspI gene can be detected by fluorescent quantitative PCR,it was found that the measured relative gene expression changes were significantly enhanced at 25℃and 16℃when analyzed with nascent RNA rather than total RNA,enabling accurate detection of RNA transcription rates.Conclusion Compared to other article reported experimental methods that utilize screening magnetic columns,the technical scheme employed in this study is more suitable for bacteria,and the operation steps are simple and easy to implement,making it an effective RNA capture method for researchers.展开更多
Objective To explore the differential expression and mechanisms of bone formation-related genes in osteoporosis(OP)leveraging bioinformatics and machine learning methodologies;and to predict the active ingredients of ...Objective To explore the differential expression and mechanisms of bone formation-related genes in osteoporosis(OP)leveraging bioinformatics and machine learning methodologies;and to predict the active ingredients of targeted traditional Chinese medicine(TCM)herbs.Methods The Gene Expression Omnibus(GEO)and GeneCards databases were employed to conduct a comprehensive screening of genes and disease-associated loci pertinent to the pathogenesis of OP.The R package was utilized as the analytical tool for the identification of differentially expressed genes.Least absolute shrinkage and selection operator(LASSO)logis-tic regression analysis and support vector machine-recursive feature elimination(SVM-RFE)algorithm were employed in defining the genetic signature specific to OP.Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analyses for the selected pivotal genes were conducted.The cell-type identification by estimating rela-tive subsets of RNA transcripts(CIBERSORT)algorithm was leveraged to examine the infiltra-tion patterns of immune cells;with Spearman’s rank correlation analysis utilized to assess the relationship between the expression levels of the genes and the presence of immune cells.Coremine Medical Database was used to screen out potential TCM herbs for the treatment of OP.Comparative Toxicogenomics Database(CTD)was employed for forecasting the TCM ac-tive ingredients targeting the key genes.AutoDock Vina 1.2.2 and GROMACS 2020 softwares were employed to conclude analysis results;facilitating the exploration of binding affinity and conformational dynamics between the TCM active ingredients and their biological targets.Results Ten genes were identified by intersecting the results from the GEO and GeneCards databases.Through the application of LASSO regression and SVM-RFE algorithm;four piv-otal genes were selected:coat protein(CP);kallikrein 3(KLK3);polymeraseγ(POLG);and transient receptor potential vanilloid 4(TRPV4).GO and KEGG pathway enrichment analy-ses revealed that these trait genes were predominantly engaged in the regulation of defense response activation;maintenance of cellular metal ion balance;and the production of chemokine ligand 5.These genes were notably associated with signaling pathways such as ferroptosis;porphyrin metabolism;and base excision repair.Immune infiltration analysis showed that key genes were highly correlated with immune cells.Macrophage M0;M1;M2;and resting dendritic cell were significantly different between groups;and there were signifi-cant differences between different groups(P<0.05).The interaction counts of resveratrol;curcumin;and quercetin with KLK3 were 7;3;and 2;respectively.It shows that the interac-tions of resveratrol;curcumin;and quercetin with KLK3 were substantial.Molecular docking and molecular dynamics simulations further confirmed the robust binding affinity of these bioactive compounds to the target genes.Conclusion Pivotal genes including CP;KLK3;POLG;and TRPV4;exhibited commendable significant prognostic value;and played a crucial role in the diagnostic assessment of OP.Resveratrol;curcumin;and quercetin;natural compounds found in TCM;showed promise in their potential to effectively modulate the bone-forming gene KLK3.This study provides a sci-entific basis for the interpretation of the pathogenesis of OP and the development of clinical drugs.展开更多
Based on mutual authentication and dense coding,a novel revised efficient quantum direct communication scheme is proposed.It is composed of two phases：the quantum state distribution process and the direct communicati...Based on mutual authentication and dense coding,a novel revised efficient quantum direct communication scheme is proposed.It is composed of two phases：the quantum state distribution process and the direct communication process.The purpose of the former is to authenticate Trent and users to each other,and let the two legitimate users（Alice and Bob）safely share the Bell states.While the latter aims to make direct communication to transmit a secret message between Alice and Bob.In order to prevent from Eve＇s eavesdropping as well as to authenticate each other simultaneously,a decoy photon checking technique is applied.Compared with other analogous protocols,the quantum state distribution process is more simple and feasible and the proposed scheme is more efficient;i.e.,the total efficiency is almost 100%.Security analysis shows that the proposed scheme is secure against the eavesdropping attacks,the impersonation attacks,and some special Trent＇s attacks,including the attacks by using different initial states.展开更多
A microelectronic circuit is used to regenerate the neural signals between the proximal end and the distal end of an injured nerve.An experimental scheme is designed and carried out to verify the feasibility of the so...A microelectronic circuit is used to regenerate the neural signals between the proximal end and the distal end of an injured nerve.An experimental scheme is designed and carried out to verify the feasibility of the so-called microelectronic neural bridge（MNB）.The sciatic signals of the source spinal toad which are evoked by chemical stimuli are used as source signals to stimulate the sciatic of the controlled spinal toad.The sciatic nerve signals of the source spinal toad,the regenerated sciatic signals in the controlled spinal toad,and the resulting electromyography（EMG）signals associated with the gastrocnemius muscle movements of the controlled spinal toad are displayed and recorded by an oscilloscope.By analyzing the coherence between the source sciatic nerve signals and the regenerated sciatic nerve signals and the coherence between the regenerated nerve signals and the EMG signals,it is proved that the regenerated sciatic nerve signals have a relationship with the source sciatic nerve signals and control shrinkage of the leg of the controlled toad.展开更多
[Objective]The aim of this study was to investigate whether communication signal C6-HSL among individual bacteria can influence plant growth and disease resistance ability. [Method]With potato virus-free plantlets as ...[Objective]The aim of this study was to investigate whether communication signal C6-HSL among individual bacteria can influence plant growth and disease resistance ability. [Method]With potato virus-free plantlets as the test materials and C6-HSL as the inducer,the potato＇s growth and the ability of resistance against Erwinia carotovora subp carotovora were tested after being treated by C6-HSL with different concentrations. The effects of C6-HSL on plant landmark defense enzyme activities and H2O2 content in potato leaves were measured especially. [Result]C6-HSL with different concentrations could obviously inhibit the growth rate of root and the number of roots,but had no effect on plant height,number of nodes and leaf size. POD or SOD activity and H2O2 content in plant landmark defense enzymes significantly increased after induction of C6-HSL,but PAL and PPO activity had no obvious change. In the resistant test,potato plants induced by C6-HSL could inhibit the infection of Erwinia carotovora subp carotovora effectively,and its incidence was significantly lower than the control group. [Conclusion]Bacteria AHL can be possibly used as a new kind of plant disease-resistant activator.展开更多
Based on the fact that the variation of tile direction of arrival (DOA) isslower than that of the channel fading, the steering vector of the desired signal is estimatedfirstly using a subspace decomposition method and...Based on the fact that the variation of tile direction of arrival (DOA) isslower than that of the channel fading, the steering vector of the desired signal is estimatedfirstly using a subspace decomposition method and then a constrained condition is configured.Traffic signals are further employed to estimate the channel vector based on the constrained leastsquares criterion. We use the iterative least squares with projection (ILSP) algorithm initializedby the pilot to get the estimation. The accuracy of channel estimation and symbol detection can beprogressively increased through the iteration procedure of the ILSP algorithm. Simulation resultsdemonstrate that the proposed algorithm improves the system performance effectively compared withthe conventional 2-D RAKE receiver.展开更多
文摘Quantum Fisher information is used to witness the quantum phase transition in a non-Hermitian trapped ion system with balanced gain and loss,from the viewpoint of quantum parameter estimation.We formulate a general non-unitary dynamic of any two-level non-Hermitian system in the form of state vector.The sudden change in the dynamics of quantum Fisher information occurs at an exceptional point characterizing quantum criticality.The dynamical behaviors of quantum Fisher information are classified into two different ways which depends on whether the system is located in symmetry unbroken or broken phase regimes.In the phase regime where parity and time reversal symmetry are unbroken,the oscillatory evolution of quantum Fisher information is presented,achieving better quantum measurement precision.In the broken phase regime,quantum Fisher information undergoes the monotonically decreasing behavior.The maximum value of quantum estimation precision is obtained at the exceptional point.It is found that the two distinct kinds of behaviors can be verified by quantum entropy and coherence.Utilizing quantum Fisher information to witness phase transition in the non-Hermitian system is emphasized.The results may have potential applications to non-Hermitian quantum information technology.
文摘Objective Detection and quantification of RNA synthesis in cells is a widely used technique for monitoring cell viability,health,and metabolic rate.After exposure to environmental stimuli,both the internal reference gene and target gene would be degraded.As a result,it is imperative to consider the accurate capture of nascent RNA and the detection of transcriptional levels of RNA following environmental stimulation.This study aims to create a Click Chemistry method that utilizes its property to capture nascent RNA from total RNA that was stimulated by the environment.Methods The new RNA was labeled with 5-ethyluridine(5-EU)instead of uracil,and the azido-biotin medium ligand was connected to the magnetic sphere using a combination of“Click Chemistry”and magnetic bead screening.Then the new RNA was captured and the transcription rate of 16S rRNA was detected by fluorescence molecular beacon(M.B.)and quantitative reverse transcription PCR(qRT-PCR).Results The bacterial nascent RNA captured by“Click Chemistry”screening can be used as a reverse transcription template to form cDNA.Combined with the fluorescent molecular beacon M.B.1,the synthesis rate of rRNA at 37℃is 1.2 times higher than that at 15℃.The 16S rRNA gene and cspI gene can be detected by fluorescent quantitative PCR,it was found that the measured relative gene expression changes were significantly enhanced at 25℃and 16℃when analyzed with nascent RNA rather than total RNA,enabling accurate detection of RNA transcription rates.Conclusion Compared to other article reported experimental methods that utilize screening magnetic columns,the technical scheme employed in this study is more suitable for bacteria,and the operation steps are simple and easy to implement,making it an effective RNA capture method for researchers.
基金National Natural Science Foundation of China(81960877).
文摘Objective To explore the differential expression and mechanisms of bone formation-related genes in osteoporosis(OP)leveraging bioinformatics and machine learning methodologies;and to predict the active ingredients of targeted traditional Chinese medicine(TCM)herbs.Methods The Gene Expression Omnibus(GEO)and GeneCards databases were employed to conduct a comprehensive screening of genes and disease-associated loci pertinent to the pathogenesis of OP.The R package was utilized as the analytical tool for the identification of differentially expressed genes.Least absolute shrinkage and selection operator(LASSO)logis-tic regression analysis and support vector machine-recursive feature elimination(SVM-RFE)algorithm were employed in defining the genetic signature specific to OP.Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analyses for the selected pivotal genes were conducted.The cell-type identification by estimating rela-tive subsets of RNA transcripts(CIBERSORT)algorithm was leveraged to examine the infiltra-tion patterns of immune cells;with Spearman’s rank correlation analysis utilized to assess the relationship between the expression levels of the genes and the presence of immune cells.Coremine Medical Database was used to screen out potential TCM herbs for the treatment of OP.Comparative Toxicogenomics Database(CTD)was employed for forecasting the TCM ac-tive ingredients targeting the key genes.AutoDock Vina 1.2.2 and GROMACS 2020 softwares were employed to conclude analysis results;facilitating the exploration of binding affinity and conformational dynamics between the TCM active ingredients and their biological targets.Results Ten genes were identified by intersecting the results from the GEO and GeneCards databases.Through the application of LASSO regression and SVM-RFE algorithm;four piv-otal genes were selected:coat protein(CP);kallikrein 3(KLK3);polymeraseγ(POLG);and transient receptor potential vanilloid 4(TRPV4).GO and KEGG pathway enrichment analy-ses revealed that these trait genes were predominantly engaged in the regulation of defense response activation;maintenance of cellular metal ion balance;and the production of chemokine ligand 5.These genes were notably associated with signaling pathways such as ferroptosis;porphyrin metabolism;and base excision repair.Immune infiltration analysis showed that key genes were highly correlated with immune cells.Macrophage M0;M1;M2;and resting dendritic cell were significantly different between groups;and there were signifi-cant differences between different groups(P<0.05).The interaction counts of resveratrol;curcumin;and quercetin with KLK3 were 7;3;and 2;respectively.It shows that the interac-tions of resveratrol;curcumin;and quercetin with KLK3 were substantial.Molecular docking and molecular dynamics simulations further confirmed the robust binding affinity of these bioactive compounds to the target genes.Conclusion Pivotal genes including CP;KLK3;POLG;and TRPV4;exhibited commendable significant prognostic value;and played a crucial role in the diagnostic assessment of OP.Resveratrol;curcumin;and quercetin;natural compounds found in TCM;showed promise in their potential to effectively modulate the bone-forming gene KLK3.This study provides a sci-entific basis for the interpretation of the pathogenesis of OP and the development of clinical drugs.
基金The National Natural Science Foundation of China(No.60873101)the Natural Science Foundation of Jiangsu Province(No.BK2008209)the Research Foundation of Nanjing University of Information Science and Technology(No.20080298)
文摘Based on mutual authentication and dense coding,a novel revised efficient quantum direct communication scheme is proposed.It is composed of two phases：the quantum state distribution process and the direct communication process.The purpose of the former is to authenticate Trent and users to each other,and let the two legitimate users（Alice and Bob）safely share the Bell states.While the latter aims to make direct communication to transmit a secret message between Alice and Bob.In order to prevent from Eve＇s eavesdropping as well as to authenticate each other simultaneously,a decoy photon checking technique is applied.Compared with other analogous protocols,the quantum state distribution process is more simple and feasible and the proposed scheme is more efficient;i.e.,the total efficiency is almost 100%.Security analysis shows that the proposed scheme is secure against the eavesdropping attacks,the impersonation attacks,and some special Trent＇s attacks,including the attacks by using different initial states.
基金The National Natural Science Foundation of China(No.90307013,90707005)the Natural Science Foundation of Jiangsu Province(No.BK2008032)+1 种基金Special Foundation and Open Foundation of State Key Laboratory of Bioelectronics of Southeast UniversityNantong Planning Project of Science and Technology(No.K2009037)
文摘A microelectronic circuit is used to regenerate the neural signals between the proximal end and the distal end of an injured nerve.An experimental scheme is designed and carried out to verify the feasibility of the so-called microelectronic neural bridge（MNB）.The sciatic signals of the source spinal toad which are evoked by chemical stimuli are used as source signals to stimulate the sciatic of the controlled spinal toad.The sciatic nerve signals of the source spinal toad,the regenerated sciatic signals in the controlled spinal toad,and the resulting electromyography（EMG）signals associated with the gastrocnemius muscle movements of the controlled spinal toad are displayed and recorded by an oscilloscope.By analyzing the coherence between the source sciatic nerve signals and the regenerated sciatic nerve signals and the coherence between the regenerated nerve signals and the EMG signals,it is proved that the regenerated sciatic nerve signals have a relationship with the source sciatic nerve signals and control shrinkage of the leg of the controlled toad.
基金Supported by Natural Science Foundation in Hebei Province(C200600707)~~
文摘[Objective]The aim of this study was to investigate whether communication signal C6-HSL among individual bacteria can influence plant growth and disease resistance ability. [Method]With potato virus-free plantlets as the test materials and C6-HSL as the inducer,the potato＇s growth and the ability of resistance against Erwinia carotovora subp carotovora were tested after being treated by C6-HSL with different concentrations. The effects of C6-HSL on plant landmark defense enzyme activities and H2O2 content in potato leaves were measured especially. [Result]C6-HSL with different concentrations could obviously inhibit the growth rate of root and the number of roots,but had no effect on plant height,number of nodes and leaf size. POD or SOD activity and H2O2 content in plant landmark defense enzymes significantly increased after induction of C6-HSL,but PAL and PPO activity had no obvious change. In the resistant test,potato plants induced by C6-HSL could inhibit the infection of Erwinia carotovora subp carotovora effectively,and its incidence was significantly lower than the control group. [Conclusion]Bacteria AHL can be possibly used as a new kind of plant disease-resistant activator.
基金The National Hi-Tech Development Plan (863-317-03-01-02-04-20).
文摘Based on the fact that the variation of tile direction of arrival (DOA) isslower than that of the channel fading, the steering vector of the desired signal is estimatedfirstly using a subspace decomposition method and then a constrained condition is configured.Traffic signals are further employed to estimate the channel vector based on the constrained leastsquares criterion. We use the iterative least squares with projection (ILSP) algorithm initializedby the pilot to get the estimation. The accuracy of channel estimation and symbol detection can beprogressively increased through the iteration procedure of the ILSP algorithm. Simulation resultsdemonstrate that the proposed algorithm improves the system performance effectively compared withthe conventional 2-D RAKE receiver.
