目的：运用生物信息学相关软件,预测欧洲女贞花粉主要过敏原Lig v 1的理化性质和结构,为Lig v 1重组表达系统的选择和过敏原性改造提供参考。方法：查询文献获得Lig v 1的氨基酸序列,运用生物信息学软件分析其理化性质（Prot Param）、...目的：运用生物信息学相关软件,预测欧洲女贞花粉主要过敏原Lig v 1的理化性质和结构,为Lig v 1重组表达系统的选择和过敏原性改造提供参考。方法：查询文献获得Lig v 1的氨基酸序列,运用生物信息学软件分析其理化性质（Prot Param）、信号肽（Signal P 4.1 Server）、跨膜区（TMHMM Server v.2.0）、二级结构（GOR4）、MHCⅡ类抗原表位（Net MHCⅡ2.2 Server）、B细胞抗原表位（ProteanTM5.01）以及系统发生树（MEGA 6）。结果：Lig v 1在大肠杆菌中稳定性较好,不存在信号肽与跨膜区;二级结构中无规则卷曲占大多数;Lig v 1潜在MHCⅡ类抗原表位为30~44区域;B细胞抗原表位既具有连续的氨基酸序列,也具有不连续的氨基酸序列;Lig v 1与欧洲白蜡以及木犀榄的同源蛋白进化距离最近。结论：大肠杆菌是适合重组Lig v 1的表达系统,Lig v 1抗原表位分析为低过敏原性改造提供参考。展开更多
猪繁殖与呼吸综合征（Porcine reproductive and respiratory syndrome,PRRS）是由猪繁殖与呼吸综合征病毒（Porcine reproductive and respiratory syndrome virus,PRRSV）引起猪的一种病毒性疾病,给养猪业造成了巨大的经济损失。
Objectives:From the view of everyday practices and the socio-technical coordination lens,this study aimed to analyz the gap between creators’intention and the users’implementation(mainly nursing staff and social wor...Objectives:From the view of everyday practices and the socio-technical coordination lens,this study aimed to analyz the gap between creators’intention and the users’implementation(mainly nursing staff and social workers)of an alert system in assisted living communities.Methods:Qualitative methods were employed by way of five user interviews and focus groups with six system developers.Modeling instruments were applied for data collection to analyze the different clinical workflows versus the expectations of the system development team.Results:Results indicate that the clinical workflow changed over time,which led to a mismatch of nurse care coordination,social practices,and technology use.The results show different mental models of the socio-technical practice.Applying the coordination theory,the following recommendations could be developed to overcome the mismatch.First,it is recommended that nursing staff set goals together.Second,a communication rhythmwith the nursing staff and developer teams should be established,with guided questions to facilitate the conversation,to shed light on the different workflows and the difference in social practices when using sensor technologies or alert systems.Third,a checklist for new employees should be created so they know how and on which devices to use the alert system.Fourth,the user experience with the alert system should be improved(e.g.,an improved user interface).Conclusions:This work indicates recommendations to close the mental model gap to overcome the mismatch between optimal use of the alert system and how the nursing staff is actually using it.展开更多
This research is dedicated to assessment of a method that was earlier proposed and developed in order to increase the degree of automation and software involvement into conceptual decision making during design of stru...This research is dedicated to assessment of a method that was earlier proposed and developed in order to increase the degree of automation and software involvement into conceptual decision making during design of structural elements of buildings. Such instruments of the theory of inventive problem solving as contradiction and function analysis and trimming formed the basis of the proposed approach that was realized in a modern building information modeling software. The common logic of the approach is also provided in the article. Qualitative research methods and particularly collecting, analyzing and interpreting data were applied in this research. Firstly, a literature review of indexed journal articles in the field of the study was performed and some trends for possible development of the topic were identified. Secondly, a survey of potential users of the methodology was conducted and analyzed. The questionnaire results showed that the suggested method and its technical realization gained attraction among respondents, however, some of them are rather cautious regarding application of the approach potentials in their practice. The paper ends with evaluation results discussion, conclusion and proposals for further research.展开更多
The objective of this paper is to measure word of mouth influence on IT career interest based on family background on Indonesian students. For this purpose the author used questionnaire as research instrument. Data as...The objective of this paper is to measure word of mouth influence on IT career interest based on family background on Indonesian students. For this purpose the author used questionnaire as research instrument. Data as a value to interest, word of mouth and family background variables, was gathered from high school students in Jakarta metropolitan. Further, data was analyzed using multiple groups on Lisrel software. Result shows that there＇s difference on the influence of word of mouth on computer/IT career interest based on family background. Model relationship between word of mouth and student interest towards computer/IT is moderated by parent education and birth order.展开更多
Ocean information management is of great importance as it has been employed in many areas of ocean science and technology. However, the developments of Ocean Information Systems(OISs) often suffer from low efficiency ...Ocean information management is of great importance as it has been employed in many areas of ocean science and technology. However, the developments of Ocean Information Systems(OISs) often suffer from low efficiency because of repetitive work and continuous modifications caused by dynamic requirements. In this paper, the basic requirements of OISs are analyzed first, and then a novel platform DPOI is proposed to improve development efficiency and enhance software quality of OISs by providing off-the-shelf resources. In the platform, the OIS is decomposed hierarchically into a set of modules, which can be reused in different system developments. These modules include the acquisition middleware and data loader that collect data from instruments and files respectively, the database that stores data consistently, the components that support fast application generation, the web services that make the data from distributed sources syntactical by use of predefined schemas and the configuration toolkit that enables software customization. With the assistance of the development platform, the software development needs no programming and the development procedure is thus accelerated greatly. We have applied the development platform in practical developments and evaluated its efficiency in several development practices and different development approaches. The results show that DPOI significantly improves development efficiency and software quality.展开更多
文摘目的：运用生物信息学相关软件,预测欧洲女贞花粉主要过敏原Lig v 1的理化性质和结构,为Lig v 1重组表达系统的选择和过敏原性改造提供参考。方法：查询文献获得Lig v 1的氨基酸序列,运用生物信息学软件分析其理化性质（Prot Param）、信号肽（Signal P 4.1 Server）、跨膜区（TMHMM Server v.2.0）、二级结构（GOR4）、MHCⅡ类抗原表位（Net MHCⅡ2.2 Server）、B细胞抗原表位（ProteanTM5.01）以及系统发生树（MEGA 6）。结果：Lig v 1在大肠杆菌中稳定性较好,不存在信号肽与跨膜区;二级结构中无规则卷曲占大多数;Lig v 1潜在MHCⅡ类抗原表位为30~44区域;B细胞抗原表位既具有连续的氨基酸序列,也具有不连续的氨基酸序列;Lig v 1与欧洲白蜡以及木犀榄的同源蛋白进化距离最近。结论：大肠杆菌是适合重组Lig v 1的表达系统,Lig v 1抗原表位分析为低过敏原性改造提供参考。
文摘猪繁殖与呼吸综合征（Porcine reproductive and respiratory syndrome,PRRS）是由猪繁殖与呼吸综合征病毒（Porcine reproductive and respiratory syndrome virus,PRRSV）引起猪的一种病毒性疾病,给养猪业造成了巨大的经济损失。
基金This work was supported by the National Library of Medicine grant #1R01LM01222.
文摘Objectives:From the view of everyday practices and the socio-technical coordination lens,this study aimed to analyz the gap between creators’intention and the users’implementation(mainly nursing staff and social workers)of an alert system in assisted living communities.Methods:Qualitative methods were employed by way of five user interviews and focus groups with six system developers.Modeling instruments were applied for data collection to analyze the different clinical workflows versus the expectations of the system development team.Results:Results indicate that the clinical workflow changed over time,which led to a mismatch of nurse care coordination,social practices,and technology use.The results show different mental models of the socio-technical practice.Applying the coordination theory,the following recommendations could be developed to overcome the mismatch.First,it is recommended that nursing staff set goals together.Second,a communication rhythmwith the nursing staff and developer teams should be established,with guided questions to facilitate the conversation,to shed light on the different workflows and the difference in social practices when using sensor technologies or alert systems.Third,a checklist for new employees should be created so they know how and on which devices to use the alert system.Fourth,the user experience with the alert system should be improved(e.g.,an improved user interface).Conclusions:This work indicates recommendations to close the mental model gap to overcome the mismatch between optimal use of the alert system and how the nursing staff is actually using it.
文摘This research is dedicated to assessment of a method that was earlier proposed and developed in order to increase the degree of automation and software involvement into conceptual decision making during design of structural elements of buildings. Such instruments of the theory of inventive problem solving as contradiction and function analysis and trimming formed the basis of the proposed approach that was realized in a modern building information modeling software. The common logic of the approach is also provided in the article. Qualitative research methods and particularly collecting, analyzing and interpreting data were applied in this research. Firstly, a literature review of indexed journal articles in the field of the study was performed and some trends for possible development of the topic were identified. Secondly, a survey of potential users of the methodology was conducted and analyzed. The questionnaire results showed that the suggested method and its technical realization gained attraction among respondents, however, some of them are rather cautious regarding application of the approach potentials in their practice. The paper ends with evaluation results discussion, conclusion and proposals for further research.
文摘The objective of this paper is to measure word of mouth influence on IT career interest based on family background on Indonesian students. For this purpose the author used questionnaire as research instrument. Data as a value to interest, word of mouth and family background variables, was gathered from high school students in Jakarta metropolitan. Further, data was analyzed using multiple groups on Lisrel software. Result shows that there＇s difference on the influence of word of mouth on computer/IT career interest based on family background. Model relationship between word of mouth and student interest towards computer/IT is moderated by parent education and birth order.
基金supported in part by National Natural Science Foundation of China under grant No. 61170258 and 61379127National Ocean Public Benefit Research Foundation under grant No. 201305033-6 and 2011 05034-10+1 种基金Marine Renewable Energy Special Foundation under grant No. GHME2012ZC02Science and Technology Development Plan of Qingdao City under Grant No. 12-1-3-81-jh
文摘Ocean information management is of great importance as it has been employed in many areas of ocean science and technology. However, the developments of Ocean Information Systems(OISs) often suffer from low efficiency because of repetitive work and continuous modifications caused by dynamic requirements. In this paper, the basic requirements of OISs are analyzed first, and then a novel platform DPOI is proposed to improve development efficiency and enhance software quality of OISs by providing off-the-shelf resources. In the platform, the OIS is decomposed hierarchically into a set of modules, which can be reused in different system developments. These modules include the acquisition middleware and data loader that collect data from instruments and files respectively, the database that stores data consistently, the components that support fast application generation, the web services that make the data from distributed sources syntactical by use of predefined schemas and the configuration toolkit that enables software customization. With the assistance of the development platform, the software development needs no programming and the development procedure is thus accelerated greatly. We have applied the development platform in practical developments and evaluated its efficiency in several development practices and different development approaches. The results show that DPOI significantly improves development efficiency and software quality.
