A mirror-image protein-based information barcoding and storage technology wherein D-amino acids are used to encode information into mirror-image proteins that are chemically synthesized is described.These mirror-image...A mirror-image protein-based information barcoding and storage technology wherein D-amino acids are used to encode information into mirror-image proteins that are chemically synthesized is described.These mirror-image proteins were then fused into various materials from which information-encoded objects were produced.Subsequently,the mirror-image proteins were extracted from the objects using biotin-streptavidin resin-mediated specific enrichment and cleaved using an Ni(Ⅱ)-mediated selective peptide cleavage.Protein sequencing was accomplished using liquid chromatography/tandem mass spectrometry(LC-MS/MS)and then transcoded into the recorded information.We demonstrated the use of this technology to encode Chinese words into mirror-image proteins,which were then fused onto a poly(ethylene terephthalate)(PET)film and retrieved and decoded by LC-MS/MS sequencing.Compared to information barcoding and storage technologies using natural biopolymers,the mirrorimage biopolymers used in our technology may be more stable and durable.展开更多
基金the National Key R&D Program of China(2017YFA0505200 and 2019YFA0706902)the National Natural Science Foundation of China(22022703,91753205,and 21750005)the Science and Technological Fund of Anhui Province for Outstanding Youth(1808085J04)。
文摘A mirror-image protein-based information barcoding and storage technology wherein D-amino acids are used to encode information into mirror-image proteins that are chemically synthesized is described.These mirror-image proteins were then fused into various materials from which information-encoded objects were produced.Subsequently,the mirror-image proteins were extracted from the objects using biotin-streptavidin resin-mediated specific enrichment and cleaved using an Ni(Ⅱ)-mediated selective peptide cleavage.Protein sequencing was accomplished using liquid chromatography/tandem mass spectrometry(LC-MS/MS)and then transcoded into the recorded information.We demonstrated the use of this technology to encode Chinese words into mirror-image proteins,which were then fused onto a poly(ethylene terephthalate)(PET)film and retrieved and decoded by LC-MS/MS sequencing.Compared to information barcoding and storage technologies using natural biopolymers,the mirrorimage biopolymers used in our technology may be more stable and durable.
