Copper nanoparticles was prepared by ascorbic acid reducing CuC12 solution modified with [ BMIm ] BF4 ionic liquid. The size of copper nanoparticles was obviously affected by the concentration of Cu2＋ . The diameter ...Copper nanoparticles was prepared by ascorbic acid reducing CuC12 solution modified with [ BMIm ] BF4 ionic liquid. The size of copper nanoparticles was obviously affected by the concentration of Cu2＋ . The diameter of nanoparticles was smaller than 10 nm when the Cu2＋ concentration was smaller than 2× 10.2 moL/L. The pH and temperature of reductive reaction had no obvious effect on the size of copper nanoparticles.展开更多
In the last few years,the overall incidence of malignant tumors has been increasing in the country.In order to find effective anti-tumor drugs,the development and modification of natural drugs have become the focus of...In the last few years,the overall incidence of malignant tumors has been increasing in the country.In order to find effective anti-tumor drugs,the development and modification of natural drugs have become the focus of domestic and international researchers.Thallus japonica,composed of polysaccharides,minerals,amino acids,natural proteins,and lipids,is a traditional Chinese medicine with the functions of eliminating phlegm and disinhibiting water.At the same time,it has the edible and medicinal efficacy.There are a lot of active components that have been demonstrated to possess diverse biological activities including anti-tumor,anti-viral,anti-coagulant,and anti-bacterial,and so on in thallus japonica.Laminarin,composed of glucose,galactose,fucose and so on,is a common and high molecular weight heteropolysaccharide in brown algae(such as thallus japonica,kelp),and it is a potential anti-tumor drug.However,the lack of biological activity of natural polysaccharides limits the application of polysaccharides,so it is necessary to modify the structure or physical and chemical properties of it to enhance the anti-tumor activity of polysaccharides.In recent years,in order to improve the biological activity of laminarin,domestic and international researchers have introduced some groups on the surface of it.Moreover,use the function of modified groups to cause the changes in physicochemical property,spatial structure,substituent group and molecular weight to improve the anti-tumor activity in vitro and in vivo.Therefore,the structural modification has become the main and powerful means to improve the activity of polysaccharides.展开更多
In this paper, we report an antibody functionalized microimmunopreci- pitation （IX IP） method used for enrich lowabundant post-translational modified （PT~ proteins. The device is fabricated by inert, nontoxic and d...In this paper, we report an antibody functionalized microimmunopreci- pitation （IX IP） method used for enrich lowabundant post-translational modified （PT~ proteins. The device is fabricated by inert, nontoxic and disposable polydimethylsiloxane （PDMS） using a silane-based chemical modification protocol, which yield antibody- terminated PDMS surfaces. In this study, the IX IP device is specifically designed for the purification of carbonylated protein, a representative example here to illustrate the potential applications for any other PTMs, which could be immuno-tagged by specific antibodies. The test model in vitro oxidized bovine serum albumin （BSA） was first derivitized by dinitrophenylhydrazide （DNPH） and then captured by the anti-DNP immobilized on this Ix lP device. The surface functional group mapping was systematically analyzed and validated by fluorescence microscopy. Quantitative study of DNP-derivatized carbonylated protein capture recovery and elution efficiency of the device was also studied. We also envision that this proteome enrichment Ix IP device can be assembled with other lab-on-a-chip components, such as microelectrophoresis or micro-chromatographic devices for follow-up protein analysis. This selective enrichment of modified proteins greatly facilitates the study of low abundant protein biomarkers discovery.展开更多
文摘Copper nanoparticles was prepared by ascorbic acid reducing CuC12 solution modified with [ BMIm ] BF4 ionic liquid. The size of copper nanoparticles was obviously affected by the concentration of Cu2＋ . The diameter of nanoparticles was smaller than 10 nm when the Cu2＋ concentration was smaller than 2× 10.2 moL/L. The pH and temperature of reductive reaction had no obvious effect on the size of copper nanoparticles.
文摘In the last few years,the overall incidence of malignant tumors has been increasing in the country.In order to find effective anti-tumor drugs,the development and modification of natural drugs have become the focus of domestic and international researchers.Thallus japonica,composed of polysaccharides,minerals,amino acids,natural proteins,and lipids,is a traditional Chinese medicine with the functions of eliminating phlegm and disinhibiting water.At the same time,it has the edible and medicinal efficacy.There are a lot of active components that have been demonstrated to possess diverse biological activities including anti-tumor,anti-viral,anti-coagulant,and anti-bacterial,and so on in thallus japonica.Laminarin,composed of glucose,galactose,fucose and so on,is a common and high molecular weight heteropolysaccharide in brown algae(such as thallus japonica,kelp),and it is a potential anti-tumor drug.However,the lack of biological activity of natural polysaccharides limits the application of polysaccharides,so it is necessary to modify the structure or physical and chemical properties of it to enhance the anti-tumor activity of polysaccharides.In recent years,in order to improve the biological activity of laminarin,domestic and international researchers have introduced some groups on the surface of it.Moreover,use the function of modified groups to cause the changes in physicochemical property,spatial structure,substituent group and molecular weight to improve the anti-tumor activity in vitro and in vivo.Therefore,the structural modification has become the main and powerful means to improve the activity of polysaccharides.
基金National Institutes of Health and the National Center for Research Resources grant number: P20RR01645 and NIH grant # DK44510
文摘In this paper, we report an antibody functionalized microimmunopreci- pitation （IX IP） method used for enrich lowabundant post-translational modified （PT~ proteins. The device is fabricated by inert, nontoxic and disposable polydimethylsiloxane （PDMS） using a silane-based chemical modification protocol, which yield antibody- terminated PDMS surfaces. In this study, the IX IP device is specifically designed for the purification of carbonylated protein, a representative example here to illustrate the potential applications for any other PTMs, which could be immuno-tagged by specific antibodies. The test model in vitro oxidized bovine serum albumin （BSA） was first derivitized by dinitrophenylhydrazide （DNPH） and then captured by the anti-DNP immobilized on this Ix lP device. The surface functional group mapping was systematically analyzed and validated by fluorescence microscopy. Quantitative study of DNP-derivatized carbonylated protein capture recovery and elution efficiency of the device was also studied. We also envision that this proteome enrichment Ix IP device can be assembled with other lab-on-a-chip components, such as microelectrophoresis or micro-chromatographic devices for follow-up protein analysis. This selective enrichment of modified proteins greatly facilitates the study of low abundant protein biomarkers discovery.
