DNA methylation is an important component of the epigenetic network, and it plays important roles in gene expression regulation and epigenetic change response to various stresses. In this study, the authors assessed t...DNA methylation is an important component of the epigenetic network, and it plays important roles in gene expression regulation and epigenetic change response to various stresses. In this study, the authors assessed the methylation patterns stressed by SCMV （sugarcane mosaic virus） in maize by methylation-sensitive amplified polymorphism （MSAP）, and identified important candidate genes related to SCMV resistance through combining microarray analysis with CpG islands prediction. The results of MSAP indicated DNA methylation levels appeared dynamic changes inoculated for 0 d, 1 d, 4 d, 5 d and 10 d. 118 candidate genes were identified infected by SCMV, which may participate in DNA methylation modification. Among them, eight candidate genes were mapped on Scmvl and Scmv2 QTL regions, which are crucial for SCMV resistance. In conclusion, DNA methylation is closely related with maize resistance to SCMV and plays an important role in regulating gene expression responded to maize resistance.展开更多
文摘DNA methylation is an important component of the epigenetic network, and it plays important roles in gene expression regulation and epigenetic change response to various stresses. In this study, the authors assessed the methylation patterns stressed by SCMV （sugarcane mosaic virus） in maize by methylation-sensitive amplified polymorphism （MSAP）, and identified important candidate genes related to SCMV resistance through combining microarray analysis with CpG islands prediction. The results of MSAP indicated DNA methylation levels appeared dynamic changes inoculated for 0 d, 1 d, 4 d, 5 d and 10 d. 118 candidate genes were identified infected by SCMV, which may participate in DNA methylation modification. Among them, eight candidate genes were mapped on Scmvl and Scmv2 QTL regions, which are crucial for SCMV resistance. In conclusion, DNA methylation is closely related with maize resistance to SCMV and plays an important role in regulating gene expression responded to maize resistance.
