In order to produce macromolecules by using the immobilized cells in microcapsules, a novel macro-porous NaCS-PDMDAAC capsules system was used to immobilize Candida valida in a semi-continuous culture mode for produci...In order to produce macromolecules by using the immobilized cells in microcapsules, a novel macro-porous NaCS-PDMDAAC capsules system was used to immobilize Candida valida in a semi-continuous culture mode for producing lipase. It was found that the encapsulation system had no negative effect on the growth and lipase productivity, and the lipase produced inside capsules could diffuse freely to the media outside capsules. The cultivation with the capsules was carried out for 15 batches and the lipase in each batch had almost the same activity. In comparison with free cell cultivation, the immobilized cell fermentation shortened obviously the period of glucose consumption from 24 hours to 8 hours and increased lipase productivity.展开更多
文摘In order to produce macromolecules by using the immobilized cells in microcapsules, a novel macro-porous NaCS-PDMDAAC capsules system was used to immobilize Candida valida in a semi-continuous culture mode for producing lipase. It was found that the encapsulation system had no negative effect on the growth and lipase productivity, and the lipase produced inside capsules could diffuse freely to the media outside capsules. The cultivation with the capsules was carried out for 15 batches and the lipase in each batch had almost the same activity. In comparison with free cell cultivation, the immobilized cell fermentation shortened obviously the period of glucose consumption from 24 hours to 8 hours and increased lipase productivity.
