以蛋白桑桑叶为研究对象,利用产朊假丝酵母菌进行培养料发酵试验。根据单因素试验结果,设计并实施了葡萄糖、磷酸二氢钾(KH2PO4)、硫酸铵[(NH4)_(2)SO_(4)]、硫酸镁(MgSO_(4))、蒸馏水五因素四水平的正交试验,发酵后测定了产朊假丝酵母...以蛋白桑桑叶为研究对象,利用产朊假丝酵母菌进行培养料发酵试验。根据单因素试验结果,设计并实施了葡萄糖、磷酸二氢钾(KH2PO4)、硫酸铵[(NH4)_(2)SO_(4)]、硫酸镁(MgSO_(4))、蒸馏水五因素四水平的正交试验,发酵后测定了产朊假丝酵母菌的菌数,确定了最佳的培养基配方,即1.2 g葡萄糖、0.025 g KH2PO4、0.05 g(NH4)_(2)SO_(4)、0.001 g MgSO_(4)、16 mL蒸馏水。在此培养基中,产朊假丝酵母菌能以菌数多达10.05亿/g进行生长繁殖。展开更多
The effects of two different phosphorus sources, corn steep liquor（CSL）and KH2PO4, on the growth of Candida glycerinogenes and glycerol production in batch fermentations were investigated. The glucose consumption in...The effects of two different phosphorus sources, corn steep liquor（CSL）and KH2PO4, on the growth of Candida glycerinogenes and glycerol production in batch fermentations were investigated. The glucose consumption in glycerol production, cell growth, maintenance, and byproducts formation during different fermentation phases was also analyzed kinetically.The results showed that the influence of CSL on cell growth and glucose consumption was so prominent that even minor variation of its quality or quantity caused much difference in experimental results. It was also found that the relationship between glycerol synthesis and cell growth was very close with CSL as phosphorus source,while it was rather loose with KH2PO4 as phosphorus source. With the increase in phosphorus concentration,glucose consumption in cell growth and maintenance was kept basically stable during the whole fermentation process. However,glucose consumption in glycerol production gradually decreased,and the byproducts which were mainly formed during the rapid growth phase increased. Further studies indicated that the yield of glycerol on glucose reached the peak of 53.44 % and 52.76 % with 14 g·L -1 CSL and 0.57 g·L -1 KH2PO4, respectively.Accordingly the yield of byproducts also decreased to the minimum value of 5.84 % and 4.25 %, respectively.展开更多
文摘以蛋白桑桑叶为研究对象,利用产朊假丝酵母菌进行培养料发酵试验。根据单因素试验结果,设计并实施了葡萄糖、磷酸二氢钾(KH2PO4)、硫酸铵[(NH4)_(2)SO_(4)]、硫酸镁(MgSO_(4))、蒸馏水五因素四水平的正交试验,发酵后测定了产朊假丝酵母菌的菌数,确定了最佳的培养基配方,即1.2 g葡萄糖、0.025 g KH2PO4、0.05 g(NH4)_(2)SO_(4)、0.001 g MgSO_(4)、16 mL蒸馏水。在此培养基中,产朊假丝酵母菌能以菌数多达10.05亿/g进行生长繁殖。
文摘The effects of two different phosphorus sources, corn steep liquor（CSL）and KH2PO4, on the growth of Candida glycerinogenes and glycerol production in batch fermentations were investigated. The glucose consumption in glycerol production, cell growth, maintenance, and byproducts formation during different fermentation phases was also analyzed kinetically.The results showed that the influence of CSL on cell growth and glucose consumption was so prominent that even minor variation of its quality or quantity caused much difference in experimental results. It was also found that the relationship between glycerol synthesis and cell growth was very close with CSL as phosphorus source,while it was rather loose with KH2PO4 as phosphorus source. With the increase in phosphorus concentration,glucose consumption in cell growth and maintenance was kept basically stable during the whole fermentation process. However,glucose consumption in glycerol production gradually decreased,and the byproducts which were mainly formed during the rapid growth phase increased. Further studies indicated that the yield of glycerol on glucose reached the peak of 53.44 % and 52.76 % with 14 g·L -1 CSL and 0.57 g·L -1 KH2PO4, respectively.Accordingly the yield of byproducts also decreased to the minimum value of 5.84 % and 4.25 %, respectively.
