The mechanism of the proton_transfer_coupled electron transfer (PT_ET) reactions between the menaquinone Q A (MQ 1) and ubiquinone Q B (UQ 1) in the bacterial photosynthetic reaction center of Rhodopseudomona vi...The mechanism of the proton_transfer_coupled electron transfer (PT_ET) reactions between the menaquinone Q A (MQ 1) and ubiquinone Q B (UQ 1) in the bacterial photosynthetic reaction center of Rhodopseudomona viridis was studied by using the B3LYP/6_31G(d) method. The changes of standard Gibbs free energy ΔG 0 of all possible reactions followed the ET reaction (1) were calculated. The results indicated that: (1) according to the ΔG 0 values of corresponding reactions, UQ 1 could not accept two electrons from MQ - 1 continually without the coupled proton transfer reactions. Because of ΔG 0 2b 0, ΔG 0 3b 0 and ΔG 0 4b 0, the corresponding PT_ET reactions could take place along with reactions (2b), (3b) and (4b) sequentially; (2) on the gaseous condition, the first and second transferred protons (H +(1) and H +(2)) from the surrounding amino acid residues or water molecules will combine with the oxygen No.7 and oxygen No.8 of UQ 1, respectively. On the condition of protein surroundings (by SCRF model, ε =4.0), the results are converse but the energy difference between the combination of H +(1) and H +(2) with UQ - 1 is quite small. The difference of ΔG 0 values between the corresponding reactions in gaseous surroundings and the SCRF model is not significant; (3) the PT_ET reactions between MQ 1 - and UQ 1 - should be as follows: MQ 1 -+UQ 1→MQ 1+UQ 1 - (1) UQ 1 - ( O (7) )+H +( HisL 190)→UQ 1H(2b) ( Gas ) or UQ 1 - ( O (8) )+H +(H 2O)→UQ 1H (2b') ( SCRF ) or UQ 1 - ( O (8) )+H + ( ArgL 217)→UQ 1H(2b') ( SCRF ) MQ 1 -+UQ 1H→MQ 1+UQ 1H - (3b) ( Gas ) MQ 1 -+UQ 1H→MQ 1+UQ 1H -(3b') ( SCR F) UQ 1H -+H +(H 2O)→UQ 1H 2(4b) ( Gas ) or UQ 1H -+H + ( ArgL 217)→UQ 1H 2 (4b) ( Gas ) or UQ 1H -+H + ( HisL 190)→UQ 1H 2 (4b') ( SCRF )展开更多
Aerobic denitrifiers were enriched by activated sludge cultivation method. By this way, 105 strains were isolated from the activated sludge and 25 strains were confirmed to be capable of obtaining energy by deoxidizat...Aerobic denitrifiers were enriched by activated sludge cultivation method. By this way, 105 strains were isolated from the activated sludge and 25 strains were confirmed to be capable of obtaining energy by deoxidization of nitrate to nitrogen gas under aerobic condition. The characteristic of one denitrifier, Pseudomonas chloritidismutans strain, was particularly studied due to its higher nitrogen removal rate. It was found that Pseudumonas chloritidismutans can use nitrite, nitrate and oxygen for aerobic respiration in liquid medium, and tbe pH increased and ORP decreased by activated denitrifier. When they used nitrite or nitrate fur respiration, nitrogen removal effect was high and nitrite could be reduced more efficiently than nitrate. Denitrification process was accomplished faster when both nitrite and nitrate existed in the medium compared to each of which existed alone. Particularly, at denitrifying activity, the nitrogen removal rate of strain was not affected by the DO concentration in the euhure media.展开更多
A novel Eucommia antifungal peptide, named EAFP3, was isolated from the bark of Eucommia ul- moides by NaC1 extract, gel filtration and reverse phase high performance liquid chromatography. The molecular mass of EAFP3...A novel Eucommia antifungal peptide, named EAFP3, was isolated from the bark of Eucommia ul- moides by NaC1 extract, gel filtration and reverse phase high performance liquid chromatography. The molecular mass of EAFP3 is 4157.3 Da, and its partial amino acid sequence is -. LYQQLIAGITLNK.-. EAFP3 exerts an inhibitory activity against Candida albicans in vitro and the drug concentration required for 50% growth inhibition (IC50) is 31.25μg/mL.展开更多
To improve the naphtha composition prediction model based on molecular type homologous series matrix (MTHS), this paper puts forward a novel molecular matrix to characterize the naphtha composition and the norreal d...To improve the naphtha composition prediction model based on molecular type homologous series matrix (MTHS), this paper puts forward a novel molecular matrix to characterize the naphtha composition and the norreal distribution hypothesis to better describe the molecular composition distribution within each homologous series of the molecular matrix. Through prediction calculation of eight groups of naphtha samples and eight groups of gasoline samples, it is verified that the normal distribution hypothesis is more applicable than gamma distribution hypothesis for the prediction model. According to the prediction results of the samples, the restrain range of normal distribution parameters during model computing process is summarized. With the bulk properties of naphtha samples and the value range of distribution parameters as input conditions, this study utilizes the improved novel molecular matrix to predict the composition of naphtha samples. As the results show, the novel molecular matrix can predict more detailed composition information of naphtha and improve prediction accuracy with less unknown parameters.展开更多
We propose a scheme for testing the small violations of the symmetrization postulate (SP) for photons in cavity QED. In the scheme, a degenerate A-type three-level atom is sent through a cavity field initially in a ...We propose a scheme for testing the small violations of the symmetrization postulate (SP) for photons in cavity QED. In the scheme, a degenerate A-type three-level atom is sent through a cavity field initially in a q-deformed coherent state. After an appropriate interaction time, the atom is measured by a state-selective detector. The probability that the atom makes a transition from one of the lower states to the other characterizes the violation of the SP. In the scheme, only one atom is required and classical fields are unnecessary, which is prior to the previous schemes.展开更多
The machine interference problem with reliable server under single vacation is considered here. There are M similar machines that are subject to fail or breaks down with a single server who is responsible for repairin...The machine interference problem with reliable server under single vacation is considered here. There are M similar machines that are subject to fail or breaks down with a single server who is responsible for repairing or maintaining the failed machines. The machine fails completely at random with rate 2 and they are serviced in order of breaks down. More so, the machines operate independently but are subject to fail or break down. The service time distributions of the failed machines are assumed to be exponentially distributed with state dependent service rate #n, where n is the number of failed machines. By state dependent service rate we mean a situation where the rate of service depends on the number of failed machines present in the system. The Chapman-Kolmogorov differential equations obtained for the reliable server under single vacation model is solved through ODE45 (Runge-Kutta algorithm of order 4 and 5) in MATLAB programming language. The transient probabilities obtained are used to compute the operational measures of performance for the systems. The following time dependent operational measures of performance for the system are obtained: expected number of failed machines, expected number of operating machines, machine availability, expected idle period, expected busy period, operational utilization of the machine, the variance of the expected number of failed machine and variance of expected number of operating machine in the system. The effects of failure rate of machines, service rate of failed machines and the number of operating machines are investigated; it is observe that the rate at which machine fails and is serviced, affect the expected number of failed and operating machines in the system. The CPU time for obtaining the transient results for the systems and the variance of the systems are reported in this work.展开更多
Production of bee products using standards of Modern technology requires high specialization and high productivity of labor adequate facilities and equipment, and coordination of natural bee potential with production ...Production of bee products using standards of Modern technology requires high specialization and high productivity of labor adequate facilities and equipment, and coordination of natural bee potential with production characteristics of the wider region. Effectively beekeeping today means using several pastures per year with a yield of 50-60 kg per hive. Mobile beekeeping is the only way to achieve high yields of production and it has considerable advantage over stationary one which is less rentable. Mobile beekeeping require higher investments and labor but with good climate and ecological conditions, one can achieve several times higher income than in stationary beekeeping. Some of the success in mobile beekeeping depends of the selection of hives. The hives must be designed to give the bees the best environment for work and development to be most efficient. Data was collected from 57 beekeepers that live in the district. Data analysis was conducted by using standard statistical and economic processes. Criteria for a recommended for the proposed beekeeping model were incomes and profit per hive. For effective beekeeping and production of bee products, especially honey, beekeeper must have adequate equipment and a good bee hives. Beekeeper must have transportation and additional equipment because their movement depends season on the season and distance to pasture.展开更多
To introduce the method and curative effect of treating pseudoaneurysms by padding and compression.Method:retrospectively analyze the curative effect of treating 17 pseudoaneurysms bypadding hard things and compressio...To introduce the method and curative effect of treating pseudoaneurysms by padding and compression.Method:retrospectively analyze the curative effect of treating 17 pseudoaneurysms bypadding hard things and compression.Result:15 of them was completely recovered by doing so,murmur disappeared and the local skin became even,there was no recurrences and complications after 0.5-3 year's following up,but the other two were the same as before the therapy.Conclusion:pseudoaneurysms can be treated effectively by padding and compression展开更多
Two experiments were conducted during 2006-2007 in Complete Randomized Design (CRD) to test the biological activity of Pseudomonas bacteria as biocides to protect sesame crop from some fungi and to evaluate its eff...Two experiments were conducted during 2006-2007 in Complete Randomized Design (CRD) to test the biological activity of Pseudomonas bacteria as biocides to protect sesame crop from some fungi and to evaluate its efficiency as plant growth promoting First experiment investigated the effects of Pseudomonas putida2 and Pseudomonas fluorescens3 on germination and seedlings growth of sesame crop against Pythium, Alternaria and Fusarium under plastic house conditions. Second experiment conducted in large pots to investigate the effects of the two bacterial isolates on some morphological, productive and physiological characters of sesame plants against same fungi under normal conditions. Results showed very low germination and slowly seedlings growth in Pythium, Fusarium and Alternaria treatments respectively, but adding bacterial vaccine of Pseudomonas putida2 and Pseudomonas fluorescens3 as a Biocide to fungi treatments, increased the germination percent and seedlings growth of sesame crop significantly, 20 days after planting compared with control treatment. In the second experiment, control treatment scored germination by 52%, while vaccines of Pseudornonas putida2 and Pseudomonas fluorescence3 treatments increased germination percentage in Fusarium, Pythium and Alternaria treatments significantly in average more than 71%. Both isolates increased significantly Leaf number per plant, leaf area per plant, height of plant, branches number per plant, total dry weight of shoot per plant and chlorophyll content compared with other and control treatments. Similar significant effect for both isolates was recorded in seeds number per pod per plant, total weight of 1,000 seeds per plant and pods number per plant and percentage of N, P, K in total dry weight of shoot per plant and oil percentage in seeds per plant compared with other and control treatments. P. putida2 increased oil percentage in seeds by 43.3, 48.0 and 45.0% respectively while with P. fluorescens3 increased to 42.7, 44.0 and 43.7% respectively compared with control treatment (27.7%). In general Pseudomonas putida2 increased most of growth characters much higher than Pseudomonas fluorescens3, it may related to siderophores compound and genetic factors.展开更多
Research on the stress gradient hypothesis recognizes that positive(i.e. facilitative) and negative(i.e. competitive) plant interactions change in intensity and effect relative to abiotic stress experienced on a gradi...Research on the stress gradient hypothesis recognizes that positive(i.e. facilitative) and negative(i.e. competitive) plant interactions change in intensity and effect relative to abiotic stress experienced on a gradient. Motivated by observations of alpine treeline ecotones, we suggest that this switch in interaction could operate along a gradient of relative size of individual plants. We propose that as neighbors increase in size relative to a focal plant they improve the environment for that plant up to a critical point. After this critical point is surpassed, however, increasing relative size of neighbors will degrade the environment such that the net interaction intensity becomes negative. We developed a conceptual(not site or species specific) individual based model to simulate a single species with recruitment, growth, and mortality dependent on the environment mediated by the relative size of neighbors. Growth and size form a feedback. Simulation results show that the size gradient model produces metrics similar to that of a stress gradient model. Visualizations reveal that the size gradient model produces spatial patterns that are similar to the complex ones observed at alpine treelines. Size-mediated interaction could be a mechanism of the stress gradient hypothesis or it could operate independent of abiotic stress.展开更多
文摘The mechanism of the proton_transfer_coupled electron transfer (PT_ET) reactions between the menaquinone Q A (MQ 1) and ubiquinone Q B (UQ 1) in the bacterial photosynthetic reaction center of Rhodopseudomona viridis was studied by using the B3LYP/6_31G(d) method. The changes of standard Gibbs free energy ΔG 0 of all possible reactions followed the ET reaction (1) were calculated. The results indicated that: (1) according to the ΔG 0 values of corresponding reactions, UQ 1 could not accept two electrons from MQ - 1 continually without the coupled proton transfer reactions. Because of ΔG 0 2b 0, ΔG 0 3b 0 and ΔG 0 4b 0, the corresponding PT_ET reactions could take place along with reactions (2b), (3b) and (4b) sequentially; (2) on the gaseous condition, the first and second transferred protons (H +(1) and H +(2)) from the surrounding amino acid residues or water molecules will combine with the oxygen No.7 and oxygen No.8 of UQ 1, respectively. On the condition of protein surroundings (by SCRF model, ε =4.0), the results are converse but the energy difference between the combination of H +(1) and H +(2) with UQ - 1 is quite small. The difference of ΔG 0 values between the corresponding reactions in gaseous surroundings and the SCRF model is not significant; (3) the PT_ET reactions between MQ 1 - and UQ 1 - should be as follows: MQ 1 -+UQ 1→MQ 1+UQ 1 - (1) UQ 1 - ( O (7) )+H +( HisL 190)→UQ 1H(2b) ( Gas ) or UQ 1 - ( O (8) )+H +(H 2O)→UQ 1H (2b') ( SCRF ) or UQ 1 - ( O (8) )+H + ( ArgL 217)→UQ 1H(2b') ( SCRF ) MQ 1 -+UQ 1H→MQ 1+UQ 1H - (3b) ( Gas ) MQ 1 -+UQ 1H→MQ 1+UQ 1H -(3b') ( SCR F) UQ 1H -+H +(H 2O)→UQ 1H 2(4b) ( Gas ) or UQ 1H -+H + ( ArgL 217)→UQ 1H 2 (4b) ( Gas ) or UQ 1H -+H + ( HisL 190)→UQ 1H 2 (4b') ( SCRF )
文摘Aerobic denitrifiers were enriched by activated sludge cultivation method. By this way, 105 strains were isolated from the activated sludge and 25 strains were confirmed to be capable of obtaining energy by deoxidization of nitrate to nitrogen gas under aerobic condition. The characteristic of one denitrifier, Pseudomonas chloritidismutans strain, was particularly studied due to its higher nitrogen removal rate. It was found that Pseudumonas chloritidismutans can use nitrite, nitrate and oxygen for aerobic respiration in liquid medium, and tbe pH increased and ORP decreased by activated denitrifier. When they used nitrite or nitrate fur respiration, nitrogen removal effect was high and nitrite could be reduced more efficiently than nitrate. Denitrification process was accomplished faster when both nitrite and nitrate existed in the medium compared to each of which existed alone. Particularly, at denitrifying activity, the nitrogen removal rate of strain was not affected by the DO concentration in the euhure media.
基金the National Natural Science Foundation of China(30660146)the Ministry of Sciences of Technology Program of China(2003CCA02600)
文摘A novel Eucommia antifungal peptide, named EAFP3, was isolated from the bark of Eucommia ul- moides by NaC1 extract, gel filtration and reverse phase high performance liquid chromatography. The molecular mass of EAFP3 is 4157.3 Da, and its partial amino acid sequence is -. LYQQLIAGITLNK.-. EAFP3 exerts an inhibitory activity against Candida albicans in vitro and the drug concentration required for 50% growth inhibition (IC50) is 31.25μg/mL.
基金Supported by the National Natural Science Foundation of China(U1462206)
文摘To improve the naphtha composition prediction model based on molecular type homologous series matrix (MTHS), this paper puts forward a novel molecular matrix to characterize the naphtha composition and the norreal distribution hypothesis to better describe the molecular composition distribution within each homologous series of the molecular matrix. Through prediction calculation of eight groups of naphtha samples and eight groups of gasoline samples, it is verified that the normal distribution hypothesis is more applicable than gamma distribution hypothesis for the prediction model. According to the prediction results of the samples, the restrain range of normal distribution parameters during model computing process is summarized. With the bulk properties of naphtha samples and the value range of distribution parameters as input conditions, this study utilizes the improved novel molecular matrix to predict the composition of naphtha samples. As the results show, the novel molecular matrix can predict more detailed composition information of naphtha and improve prediction accuracy with less unknown parameters.
文摘We propose a scheme for testing the small violations of the symmetrization postulate (SP) for photons in cavity QED. In the scheme, a degenerate A-type three-level atom is sent through a cavity field initially in a q-deformed coherent state. After an appropriate interaction time, the atom is measured by a state-selective detector. The probability that the atom makes a transition from one of the lower states to the other characterizes the violation of the SP. In the scheme, only one atom is required and classical fields are unnecessary, which is prior to the previous schemes.
文摘The machine interference problem with reliable server under single vacation is considered here. There are M similar machines that are subject to fail or breaks down with a single server who is responsible for repairing or maintaining the failed machines. The machine fails completely at random with rate 2 and they are serviced in order of breaks down. More so, the machines operate independently but are subject to fail or break down. The service time distributions of the failed machines are assumed to be exponentially distributed with state dependent service rate #n, where n is the number of failed machines. By state dependent service rate we mean a situation where the rate of service depends on the number of failed machines present in the system. The Chapman-Kolmogorov differential equations obtained for the reliable server under single vacation model is solved through ODE45 (Runge-Kutta algorithm of order 4 and 5) in MATLAB programming language. The transient probabilities obtained are used to compute the operational measures of performance for the systems. The following time dependent operational measures of performance for the system are obtained: expected number of failed machines, expected number of operating machines, machine availability, expected idle period, expected busy period, operational utilization of the machine, the variance of the expected number of failed machine and variance of expected number of operating machine in the system. The effects of failure rate of machines, service rate of failed machines and the number of operating machines are investigated; it is observe that the rate at which machine fails and is serviced, affect the expected number of failed and operating machines in the system. The CPU time for obtaining the transient results for the systems and the variance of the systems are reported in this work.
文摘Production of bee products using standards of Modern technology requires high specialization and high productivity of labor adequate facilities and equipment, and coordination of natural bee potential with production characteristics of the wider region. Effectively beekeeping today means using several pastures per year with a yield of 50-60 kg per hive. Mobile beekeeping is the only way to achieve high yields of production and it has considerable advantage over stationary one which is less rentable. Mobile beekeeping require higher investments and labor but with good climate and ecological conditions, one can achieve several times higher income than in stationary beekeeping. Some of the success in mobile beekeeping depends of the selection of hives. The hives must be designed to give the bees the best environment for work and development to be most efficient. Data was collected from 57 beekeepers that live in the district. Data analysis was conducted by using standard statistical and economic processes. Criteria for a recommended for the proposed beekeeping model were incomes and profit per hive. For effective beekeeping and production of bee products, especially honey, beekeeper must have adequate equipment and a good bee hives. Beekeeper must have transportation and additional equipment because their movement depends season on the season and distance to pasture.
文摘To introduce the method and curative effect of treating pseudoaneurysms by padding and compression.Method:retrospectively analyze the curative effect of treating 17 pseudoaneurysms bypadding hard things and compression.Result:15 of them was completely recovered by doing so,murmur disappeared and the local skin became even,there was no recurrences and complications after 0.5-3 year's following up,but the other two were the same as before the therapy.Conclusion:pseudoaneurysms can be treated effectively by padding and compression
文摘Two experiments were conducted during 2006-2007 in Complete Randomized Design (CRD) to test the biological activity of Pseudomonas bacteria as biocides to protect sesame crop from some fungi and to evaluate its efficiency as plant growth promoting First experiment investigated the effects of Pseudomonas putida2 and Pseudomonas fluorescens3 on germination and seedlings growth of sesame crop against Pythium, Alternaria and Fusarium under plastic house conditions. Second experiment conducted in large pots to investigate the effects of the two bacterial isolates on some morphological, productive and physiological characters of sesame plants against same fungi under normal conditions. Results showed very low germination and slowly seedlings growth in Pythium, Fusarium and Alternaria treatments respectively, but adding bacterial vaccine of Pseudomonas putida2 and Pseudomonas fluorescens3 as a Biocide to fungi treatments, increased the germination percent and seedlings growth of sesame crop significantly, 20 days after planting compared with control treatment. In the second experiment, control treatment scored germination by 52%, while vaccines of Pseudornonas putida2 and Pseudomonas fluorescence3 treatments increased germination percentage in Fusarium, Pythium and Alternaria treatments significantly in average more than 71%. Both isolates increased significantly Leaf number per plant, leaf area per plant, height of plant, branches number per plant, total dry weight of shoot per plant and chlorophyll content compared with other and control treatments. Similar significant effect for both isolates was recorded in seeds number per pod per plant, total weight of 1,000 seeds per plant and pods number per plant and percentage of N, P, K in total dry weight of shoot per plant and oil percentage in seeds per plant compared with other and control treatments. P. putida2 increased oil percentage in seeds by 43.3, 48.0 and 45.0% respectively while with P. fluorescens3 increased to 42.7, 44.0 and 43.7% respectively compared with control treatment (27.7%). In general Pseudomonas putida2 increased most of growth characters much higher than Pseudomonas fluorescens3, it may related to siderophores compound and genetic factors.
基金based upon work while an author served at the National Science Foundation(USA)
文摘Research on the stress gradient hypothesis recognizes that positive(i.e. facilitative) and negative(i.e. competitive) plant interactions change in intensity and effect relative to abiotic stress experienced on a gradient. Motivated by observations of alpine treeline ecotones, we suggest that this switch in interaction could operate along a gradient of relative size of individual plants. We propose that as neighbors increase in size relative to a focal plant they improve the environment for that plant up to a critical point. After this critical point is surpassed, however, increasing relative size of neighbors will degrade the environment such that the net interaction intensity becomes negative. We developed a conceptual(not site or species specific) individual based model to simulate a single species with recruitment, growth, and mortality dependent on the environment mediated by the relative size of neighbors. Growth and size form a feedback. Simulation results show that the size gradient model produces metrics similar to that of a stress gradient model. Visualizations reveal that the size gradient model produces spatial patterns that are similar to the complex ones observed at alpine treelines. Size-mediated interaction could be a mechanism of the stress gradient hypothesis or it could operate independent of abiotic stress.