AIM: To present an approach for selectively killing retrovirus-infected cells that combines the toxicity of Pseudomonas exotoxin (PE) and the presence of reverse transcriptase (RT) in infected cells. METHODS: PE antis...AIM: To present an approach for selectively killing retrovirus-infected cells that combines the toxicity of Pseudomonas exotoxin (PE) and the presence of reverse transcriptase (RT) in infected cells. METHODS: PE antisense toxin RNA has palindromic stem loops at its 5' and 3' ends enabling self-primed generation of cDNA in the presence of RT. The RT activity expressed in retrovirus-infected cells converts "antisense-toxin-RNA" into a lethal toxin gene exclusively in these cells. RESULTS: Using cotransfection studies with PE-expressing RNAs and β-gal expressing reporter plasmids, we show that, in HepG2 and HepG2.2.15 hepatoma cells as well as in duck hepatitis B virus (DHBV) infected cells, HBV or DHBV-polymerase reverse transcribe a lethal cDNA copy of an antisense toxin RNA, which is composed of sequences complementary to a PE gene and eukaryotic transcription and translation signals. CONCLUSION: This finding may have important implications as a novel therapeutic strategy aimed at the elimination of HBV infection.展开更多
In the present investigation an attempt has been made to demineralize the Gondwana coal of Gopinathpur top and bottom seams of Mugma mine, Raniganj coalfield, Dhanbad with the help of Pseudomonas mendocina strain B6-1...In the present investigation an attempt has been made to demineralize the Gondwana coal of Gopinathpur top and bottom seams of Mugma mine, Raniganj coalfield, Dhanbad with the help of Pseudomonas mendocina strain B6-1. The change in the amount of ash yield and decrease in the concentration of selected minor elements like Na, K, Mn and Ca and environmentally sensitive selected trace elements such as Cd, Pb, Se, Ni, Mn, and Zn have been studied as a function of time of bacterial treatment as well as with variation in the bacterial biomass. After 28 days of bacterial treatment there was variable amount of decrease observed in ash yield as well as in the concentration of minor and trace elements. The removal of the elements was further enhanced with the increase in the bacterial biomass from 10 to 25 mg/mL. Due to over exploitation of superior grade coals in the country, the remaining coal resources, available for current use, are inferior in grade and contain high level of impurities and there is ample scope of bio-beneficiation of these coals using bacterial biomass.展开更多
A new Shift bases was synthesized by the reaction of p-dimetyl-amino-benzylaldehide and α-naftyl-amine. All synthesized compounds were characterized by elemental analysis, IR （infra red） spectroscopy and NMR （nucl...A new Shift bases was synthesized by the reaction of p-dimetyl-amino-benzylaldehide and α-naftyl-amine. All synthesized compounds were characterized by elemental analysis, IR （infra red） spectroscopy and NMR （nuclear magnetic resonance） spectroscopy and evaluated for their in vitro antifungal activities of the compounds tested against Pseudomonas aeruginosa, Mycobacterium lacticolium, Aspergillus niger, Cladasporium resinale, Penicillium chrosegenum, Chastorniurn gloloodium and Trichoderma virideh. These derivatives have vast range of biological activities which benefit us.展开更多
文摘AIM: To present an approach for selectively killing retrovirus-infected cells that combines the toxicity of Pseudomonas exotoxin (PE) and the presence of reverse transcriptase (RT) in infected cells. METHODS: PE antisense toxin RNA has palindromic stem loops at its 5' and 3' ends enabling self-primed generation of cDNA in the presence of RT. The RT activity expressed in retrovirus-infected cells converts "antisense-toxin-RNA" into a lethal toxin gene exclusively in these cells. RESULTS: Using cotransfection studies with PE-expressing RNAs and β-gal expressing reporter plasmids, we show that, in HepG2 and HepG2.2.15 hepatoma cells as well as in duck hepatitis B virus (DHBV) infected cells, HBV or DHBV-polymerase reverse transcribe a lethal cDNA copy of an antisense toxin RNA, which is composed of sequences complementary to a PE gene and eukaryotic transcription and translation signals. CONCLUSION: This finding may have important implications as a novel therapeutic strategy aimed at the elimination of HBV infection.
文摘In the present investigation an attempt has been made to demineralize the Gondwana coal of Gopinathpur top and bottom seams of Mugma mine, Raniganj coalfield, Dhanbad with the help of Pseudomonas mendocina strain B6-1. The change in the amount of ash yield and decrease in the concentration of selected minor elements like Na, K, Mn and Ca and environmentally sensitive selected trace elements such as Cd, Pb, Se, Ni, Mn, and Zn have been studied as a function of time of bacterial treatment as well as with variation in the bacterial biomass. After 28 days of bacterial treatment there was variable amount of decrease observed in ash yield as well as in the concentration of minor and trace elements. The removal of the elements was further enhanced with the increase in the bacterial biomass from 10 to 25 mg/mL. Due to over exploitation of superior grade coals in the country, the remaining coal resources, available for current use, are inferior in grade and contain high level of impurities and there is ample scope of bio-beneficiation of these coals using bacterial biomass.
文摘A new Shift bases was synthesized by the reaction of p-dimetyl-amino-benzylaldehide and α-naftyl-amine. All synthesized compounds were characterized by elemental analysis, IR （infra red） spectroscopy and NMR （nuclear magnetic resonance） spectroscopy and evaluated for their in vitro antifungal activities of the compounds tested against Pseudomonas aeruginosa, Mycobacterium lacticolium, Aspergillus niger, Cladasporium resinale, Penicillium chrosegenum, Chastorniurn gloloodium and Trichoderma virideh. These derivatives have vast range of biological activities which benefit us.
