应用停流时间分辨光谱研究氧合血红蛋白与过氧化氢反应中间体的动力学和机理

Interaction between oxyhemoglobin and H2O2 was studied by the aid of stopped-flow time-resolved spectrophotometry. The consecutive reaction kinetics of oxyhemoglobin with H2O2 suggested the presence of an intermediate species. The influence of pH, concentration of H2O2, and temperature on the pseudo-first order rate constants was studied. The kinetic experiments performed at different temperatures allowed to calculate the activation energy. A reasonable mechanism was also proposed.

氧合血红蛋白与亚硝酸根、过氧化亚硝酸离子反应的动力学研究

利用快速混合停流时间分辨光谱研究了氧合血红蛋白与亚硝酸根的反应 .研究表明氧合血红蛋白和NO-2 的反应历程比较特殊 ,根据实验结果提出了合理的反应机理 .同时与氧合血红蛋白和过氧化亚硝酸离子的反应进行了对比 ,发现氧合血红蛋白和过氧化亚硝酸离子的反应具有双相反应动力学的特征 。