外涵静子叶片是大涵道比涡扇发动机气路的核心部件之一,外涵静子脱出是一种较为严重的故障模式,此故障可能会导致飞机或其他发动机部件损伤,进而造成灾难性事故。对外涵静子叶片脱出故障的预警是一项重要的工作。但因其早期特征不明显,...外涵静子叶片是大涵道比涡扇发动机气路的核心部件之一,外涵静子脱出是一种较为严重的故障模式,此故障可能会导致飞机或其他发动机部件损伤,进而造成灾难性事故。对外涵静子叶片脱出故障的预警是一项重要的工作。但因其早期特征不明显,现有的方法较难对此类故障进行有效的预警。因此,针对该问题,基于监控数据提出一种深度特征提取的支持向量数据域描述(Support vector data description,SVDD)的故障预警方法,以实现对外涵静子叶片脱出故障的早期预警。首先,采用基于发动机气路性能辨识的建模方法,建立发动机特定性能参数的观测模型对气路参数进行深度特征提取,以真实状态量与模型观测量的差值作为航空发动机是否发生故障的特征;然后利用SVDD算法建立决策边界,实现故障数据的自动划分,决策边界生成的阈值可在故障发生之前的一定时间之内给出告警;最后,经过多次计算,结果表明,在故障早期直至故障发生的区间内,表征其健康状态的性能参数都与观测量有较大的偏移,表明了所选特征的有效性。使用数据增强方法生成故障仿真数据与真实数据进行对比验证,预警时间比故障真实发生时间预警模型平均提前3.14 h。展开更多
Aiming at the problem of on-line damage diagnosis in structural health monitoring (SHM), an algorithm of feature extraction and damage alarming based on auto-regressive moving-average (ARMA) time series analysis i...Aiming at the problem of on-line damage diagnosis in structural health monitoring (SHM), an algorithm of feature extraction and damage alarming based on auto-regressive moving-average (ARMA) time series analysis is presented. The monitoring data were first modeled as ARMA models, while a principalcomponent matrix derived from the AR coefficients of these models was utilized to establish the Mahalanobisdistance criterion functions. Then, a new damage-sensitive feature index DDSF is proposed. A hypothesis test involving the t-test method is further applied to obtain a decision of damage alarming as the mean value of DDSF had significantly changed after damage. The numerical results of a three-span-girder model shows that the defined index is sensitive to subtle structural damage, and the proposed algorithm can be applied to the on-line damage alarming in SHM.展开更多
A thorough understanding of the normal bacterial flora associated with shrimp larviculture systems contributes to probiotic screening and disease control. The bacterial community of the water column over a commercial ...A thorough understanding of the normal bacterial flora associated with shrimp larviculture systems contributes to probiotic screening and disease control. The bacterial community of the water column over a commercial Litopenaeus vannamei larval rearing run was characterized with both culture-dependent and culture-independent methods. A total of 27 phylotypes at the species level were isolated and identified based on 16S rDNA sequence analysis. Denaturing gradient gel electrophoresis (DGGE) analysis of the V3-V5 region of 16S rRNA genes showed a dynamic bacterial community with major changes occurred from stages zoea to mysis during the rearing run. The sequences retrieved were affiliated to four phyla, Proteobacteria, Actinobacteria, Bacteroidetes, and Firmicutes, with the family Rhodobacteraceae being the most frequently recovered one. Subsequently, 13 representative strains conferred higher larval survival than the control when evaluated in the in-vivo experiments; in particular, three candidates, assigned to Phaeobacter sp., Arthrobacter sp., and Microbacteriurn sp., significantly improved larval survival (P〈0.05). Therefore, the healthy shrimp larviculture system harbored a diverse and favorable bacterial flora, which contribute to larval development and are of great importance in exploiting novel probiotics.展开更多
Coronaviruses, such as severe acute respiratory syndrome coronavirus and Middle East respiratory syndrome coronavirus, pose significant public health threats. Bats have been suggested to act as natural reservoirs for ...Coronaviruses, such as severe acute respiratory syndrome coronavirus and Middle East respiratory syndrome coronavirus, pose significant public health threats. Bats have been suggested to act as natural reservoirs for both these viruses, and periodic monitoring of coronaviruses in bats may thus provide important clues about emergent infectious viruses. The Eastern bent-wing bat Miniopterus fuliginosus is distributed extensively throughout China. We therefore analyzed the genetic diversity of coronaviruses in samples of M. fuliginosus collected from nine Chinese provinces during 2011–2013. The only coronavirus genus found was Alphacoronavirus. We established six complete and five partial genomic sequences of alphacoronaviruses, which revealed that they could be divided into two distinct lineages, with close relationships to coronaviruses in Miniopterus magnater and Miniopterus pusillus. Recombination was confirmed by detecting putative breakpoints of Lineage 1 coronaviruses in M. fuliginosus and M. pusillus(Wu et al., 2015), which supported the results of topological and phylogenetic analyses. The established alphacoronavirus genome sequences showed high similarity to other alphacoronaviruses found in other Miniopterus species, suggesting that their transmission in different Miniopterus species may provide opportunities for recombination with different alphacoronaviruses. The genetic information for these novel alphacoronaviruses will improve our understanding of the evolution and genetic diversity of coronaviruses, with potentially important implications for the transmission of human diseases.展开更多
文摘外涵静子叶片是大涵道比涡扇发动机气路的核心部件之一,外涵静子脱出是一种较为严重的故障模式,此故障可能会导致飞机或其他发动机部件损伤,进而造成灾难性事故。对外涵静子叶片脱出故障的预警是一项重要的工作。但因其早期特征不明显,现有的方法较难对此类故障进行有效的预警。因此,针对该问题,基于监控数据提出一种深度特征提取的支持向量数据域描述(Support vector data description,SVDD)的故障预警方法,以实现对外涵静子叶片脱出故障的早期预警。首先,采用基于发动机气路性能辨识的建模方法,建立发动机特定性能参数的观测模型对气路参数进行深度特征提取,以真实状态量与模型观测量的差值作为航空发动机是否发生故障的特征;然后利用SVDD算法建立决策边界,实现故障数据的自动划分,决策边界生成的阈值可在故障发生之前的一定时间之内给出告警;最后,经过多次计算,结果表明,在故障早期直至故障发生的区间内,表征其健康状态的性能参数都与观测量有较大的偏移,表明了所选特征的有效性。使用数据增强方法生成故障仿真数据与真实数据进行对比验证,预警时间比故障真实发生时间预警模型平均提前3.14 h。
基金The National High Technology Research and Devel-opment Program of China (863Program) (No2006AA04Z416)the National Natural Science Foundation of China (No50538020)
文摘Aiming at the problem of on-line damage diagnosis in structural health monitoring (SHM), an algorithm of feature extraction and damage alarming based on auto-regressive moving-average (ARMA) time series analysis is presented. The monitoring data were first modeled as ARMA models, while a principalcomponent matrix derived from the AR coefficients of these models was utilized to establish the Mahalanobisdistance criterion functions. Then, a new damage-sensitive feature index DDSF is proposed. A hypothesis test involving the t-test method is further applied to obtain a decision of damage alarming as the mean value of DDSF had significantly changed after damage. The numerical results of a three-span-girder model shows that the defined index is sensitive to subtle structural damage, and the proposed algorithm can be applied to the on-line damage alarming in SHM.
基金Supported by the National Natural Science Foundation of China(No.31372536)the Special Projects of Science and Technology Promotion for Marine Fisheries in Guangdong Province(No.A201008B03)the Startup Programs for Introduction Personnel of Guangdong Ocean University(Nos.E10046,E11328)
文摘A thorough understanding of the normal bacterial flora associated with shrimp larviculture systems contributes to probiotic screening and disease control. The bacterial community of the water column over a commercial Litopenaeus vannamei larval rearing run was characterized with both culture-dependent and culture-independent methods. A total of 27 phylotypes at the species level were isolated and identified based on 16S rDNA sequence analysis. Denaturing gradient gel electrophoresis (DGGE) analysis of the V3-V5 region of 16S rRNA genes showed a dynamic bacterial community with major changes occurred from stages zoea to mysis during the rearing run. The sequences retrieved were affiliated to four phyla, Proteobacteria, Actinobacteria, Bacteroidetes, and Firmicutes, with the family Rhodobacteraceae being the most frequently recovered one. Subsequently, 13 representative strains conferred higher larval survival than the control when evaluated in the in-vivo experiments; in particular, three candidates, assigned to Phaeobacter sp., Arthrobacter sp., and Microbacteriurn sp., significantly improved larval survival (P〈0.05). Therefore, the healthy shrimp larviculture system harbored a diverse and favorable bacterial flora, which contribute to larval development and are of great importance in exploiting novel probiotics.
基金supported by the Program for Changjiang Scholars and Innovative Research Team in University of China (IRT13007)the National S&T Major Project “China Mega-Project for Infectious Disease” (2011ZX10004-001, 2014ZX10004001) from China+1 种基金the National Natural Science Foundation of China (81501773)the PUMC Youth Fund and Fundamental Research Funds for the Central Universities (3332015095, 3332015006)
文摘Coronaviruses, such as severe acute respiratory syndrome coronavirus and Middle East respiratory syndrome coronavirus, pose significant public health threats. Bats have been suggested to act as natural reservoirs for both these viruses, and periodic monitoring of coronaviruses in bats may thus provide important clues about emergent infectious viruses. The Eastern bent-wing bat Miniopterus fuliginosus is distributed extensively throughout China. We therefore analyzed the genetic diversity of coronaviruses in samples of M. fuliginosus collected from nine Chinese provinces during 2011–2013. The only coronavirus genus found was Alphacoronavirus. We established six complete and five partial genomic sequences of alphacoronaviruses, which revealed that they could be divided into two distinct lineages, with close relationships to coronaviruses in Miniopterus magnater and Miniopterus pusillus. Recombination was confirmed by detecting putative breakpoints of Lineage 1 coronaviruses in M. fuliginosus and M. pusillus(Wu et al., 2015), which supported the results of topological and phylogenetic analyses. The established alphacoronavirus genome sequences showed high similarity to other alphacoronaviruses found in other Miniopterus species, suggesting that their transmission in different Miniopterus species may provide opportunities for recombination with different alphacoronaviruses. The genetic information for these novel alphacoronaviruses will improve our understanding of the evolution and genetic diversity of coronaviruses, with potentially important implications for the transmission of human diseases.