A thorough understanding of the normal bacterial flora associated with shrimp larviculture systems contributes to probiotic screening and disease control. The bacterial community of the water column over a commercial ...A thorough understanding of the normal bacterial flora associated with shrimp larviculture systems contributes to probiotic screening and disease control. The bacterial community of the water column over a commercial Litopenaeus vannamei larval rearing run was characterized with both culture-dependent and culture-independent methods. A total of 27 phylotypes at the species level were isolated and identified based on 16S rDNA sequence analysis. Denaturing gradient gel electrophoresis （DGGE） analysis of the V3-V5 region of 16S rRNA genes showed a dynamic bacterial community with major changes occurred from stages zoea to mysis during the rearing run. The sequences retrieved were affiliated to four phyla, Proteobacteria, Actinobacteria, Bacteroidetes, and Firmicutes, with the family Rhodobacteraceae being the most frequently recovered one. Subsequently, 13 representative strains conferred higher larval survival than the control when evaluated in the in-vivo experiments; in particular, three candidates, assigned to Phaeobacter sp., Arthrobacter sp., and Microbacteriurn sp., significantly improved larval survival （P〈0.05）. Therefore, the healthy shrimp larviculture system harbored a diverse and favorable bacterial flora, which contribute to larval development and are of great importance in exploiting novel probiotics.展开更多
This paper discribes the definition of ecosystem health for the water-level flutuation zone of the Three Gorges Region and puts forward an evaluation system involving indicators in three groups: 1) structural indicato...This paper discribes the definition of ecosystem health for the water-level flutuation zone of the Three Gorges Region and puts forward an evaluation system involving indicators in three groups: 1) structural indicators comprise slope, biodiversity, environmental capacity, stability, restoration ability and damage situation; 2) functional indicators including probability of geological hazard, erosion rate, habitat rate, land use intension and days of tourist season; 3) environmental indicatiors made up of population quality, potential intension of human, ground water quality, ambient air quality, wastewater treatment rate, pesticide use rate, fertilizer use rate, environmental management and public participation. In the design of the system, the subject zone is regarded as the type similar to wetland and the impacts of human activities on the zone are attached great importance to.展开更多
基金Supported by the National Natural Science Foundation of China(No.31372536)the Special Projects of Science and Technology Promotion for Marine Fisheries in Guangdong Province(No.A201008B03)the Startup Programs for Introduction Personnel of Guangdong Ocean University(Nos.E10046,E11328)
文摘A thorough understanding of the normal bacterial flora associated with shrimp larviculture systems contributes to probiotic screening and disease control. The bacterial community of the water column over a commercial Litopenaeus vannamei larval rearing run was characterized with both culture-dependent and culture-independent methods. A total of 27 phylotypes at the species level were isolated and identified based on 16S rDNA sequence analysis. Denaturing gradient gel electrophoresis （DGGE） analysis of the V3-V5 region of 16S rRNA genes showed a dynamic bacterial community with major changes occurred from stages zoea to mysis during the rearing run. The sequences retrieved were affiliated to four phyla, Proteobacteria, Actinobacteria, Bacteroidetes, and Firmicutes, with the family Rhodobacteraceae being the most frequently recovered one. Subsequently, 13 representative strains conferred higher larval survival than the control when evaluated in the in-vivo experiments; in particular, three candidates, assigned to Phaeobacter sp., Arthrobacter sp., and Microbacteriurn sp., significantly improved larval survival （P〈0.05）. Therefore, the healthy shrimp larviculture system harbored a diverse and favorable bacterial flora, which contribute to larval development and are of great importance in exploiting novel probiotics.
文摘This paper discribes the definition of ecosystem health for the water-level flutuation zone of the Three Gorges Region and puts forward an evaluation system involving indicators in three groups: 1) structural indicators comprise slope, biodiversity, environmental capacity, stability, restoration ability and damage situation; 2) functional indicators including probability of geological hazard, erosion rate, habitat rate, land use intension and days of tourist season; 3) environmental indicatiors made up of population quality, potential intension of human, ground water quality, ambient air quality, wastewater treatment rate, pesticide use rate, fertilizer use rate, environmental management and public participation. In the design of the system, the subject zone is regarded as the type similar to wetland and the impacts of human activities on the zone are attached great importance to.
