目的探讨健脾益气活血汤治疗2型糖尿病(type 2 diabetes mellitus,T2DM)合并骨质疏松的疗效。方法选取100例河北省沧州中西医结合医院收治的T2DM合并骨质疏松患者,分为对照组(50例)和研究组(50例),分组方法为随机数字表法,选例时间设置...目的探讨健脾益气活血汤治疗2型糖尿病(type 2 diabetes mellitus,T2DM)合并骨质疏松的疗效。方法选取100例河北省沧州中西医结合医院收治的T2DM合并骨质疏松患者,分为对照组(50例)和研究组(50例),分组方法为随机数字表法,选例时间设置在2022年1月—2022年10月。对照组接受常规西医方案治疗,研究组在对照组的基础上接受健脾益气活血汤治疗,两组患者连续治疗6个月。比较两组治疗后临床疗效,治疗前后骨代谢指标、腰椎、双髋部骨密度、疼痛程度、中医症状积分以及血糖指标。结果治疗后,研究组总有效率高于对照组;治疗后与治疗前比较,两组血清总Ⅰ型胶原氨基端延长肽(peptide of type I collagen,PINP)、β胶原降解产物(β-collagen degradation product,β-CTX)、I型胶原C末端肽(C-terminal peptide of type I collagen,CTX-1)、空腹血糖(fasting blood glucose,FBG)、餐后2 h血糖(2 h postprandial blood glucose,2 h PG)、糖化血红蛋白(glycosylated hemoglobin,HbA1c)水平、视觉模拟评分法(visual analogue scale,VAS)评分及各项中医症状积分降低,两组间治疗后相比,研究组更低;治疗后与治疗前比较,两组血清碱性磷酸酶(alkaline phosphatase,ALP)、护骨素(osteoprotegerin,OPG)及腰椎、双髋部骨密度水平升高,两组间治疗后相比,研究组水平更高,数据存在较显著的差异(P<0.05)。结论健脾益气活血汤治疗T2DM合并骨质疏松患者有助于缓解其临床症状,优化骨代谢指标,增加腰椎、双髋部骨密度,减轻疼痛,改善血糖指标,疗效显著。展开更多
AIM： To evaluate the effect of Chinese traditional medicinal prescription, JIANPI HUOXUE decoction （JHD） on cytokine secretion pathway in rat liver induced by lipopolysaccharide （LPS）. METHODS： Twenty-four male ...AIM： To evaluate the effect of Chinese traditional medicinal prescription, JIANPI HUOXUE decoction （JHD） on cytokine secretion pathway in rat liver induced by lipopolysaccharide （LPS）. METHODS： Twenty-four male SD rats were divided into normal group （n = 4）, model group （n = 10） and JHD group （n = 10） randomly. Rats in model group and JHD group were administrated with normal saline or JHD via gastrogavage respectively twice a day for 3 d. One hour after the last administration, rats were injected with LPS via tail vein, 50 μg/kg. Simultaneously, rats in normal group were injected with equivalent normal saline. After LPS stimulation for 1.5 h, serum and liver tissue were collected. Pathological change of liver tissues was observed through hematoxylineosin （H.E.） staining. Tumor necrosis factor alpha （TNF-α） in serum were assayed by enzyme linked immunosorbent assay （ELISA）. The protein expression of TNF-α, phosphorylated inhibit-κB （p-κB） and CD68 in liver were assayed by Western blot. The distribution of CD68 protein in liver was observed through immunohistochemical staining. The mRNA expression of TNF-α, interleukin-6 （IL-6）, CD14, toll-like receptor 2 （TLR2） and TLR4 in liver were assayed by real-time RT-PCR.RESULTS： Predominant microvesicular change, hepatocyte tumefaction and cytoplasm dilution were observed in liver tissues after LPS administration as well as obvious CD68 positive staining in hepatic sinusoidal. After LPS stimulation, serum TNF-α （31.35 ± 6.06 vs 12225.40 ± 9007.03, P 〈 0.05）, protein expression of CD68 （1.13 ± 0.49 vs 3.36 ±1.69, P 〈 0.05）, p-IκB （0.01 ±0.01 vs 2.07 ＋ 0.83, P 〈 0.01） and TNF-α （0.27 ± 0.13 vs 1.29 ± 0.37, P 〈 0.01） in liver and mRNA expression of TNF-α （1.96 ± 2.23 vs 21.45 ±6.00, P 〈 0.01）, IL-6 （4.80 ± 6.42 vs 193.50 ± 36.36, P 〈 0.01） and TLR2 （1.44 ± 0.62 vs 4.16 ± 0.08, P 〈 0.01） in liver were also increased significantly. These pathological changes were all improved in .1HD group. On the other hand, TLR4 mRNA （1.22 ± 0.30 vs 0.50 ± 0.15, P 〈 0.05） was down-regulated and CD14 mRNA increased but not significantly after LPS stimulation. CONCLUSION： JHD can inhibit cytokine secretion pathway induced by LPS in rat liver, which is probably associated with its regulation on CD68, p-IκB and endotoxin receptor TLR2.展开更多
文摘目的探讨健脾益气活血汤治疗2型糖尿病(type 2 diabetes mellitus,T2DM)合并骨质疏松的疗效。方法选取100例河北省沧州中西医结合医院收治的T2DM合并骨质疏松患者,分为对照组(50例)和研究组(50例),分组方法为随机数字表法,选例时间设置在2022年1月—2022年10月。对照组接受常规西医方案治疗,研究组在对照组的基础上接受健脾益气活血汤治疗,两组患者连续治疗6个月。比较两组治疗后临床疗效,治疗前后骨代谢指标、腰椎、双髋部骨密度、疼痛程度、中医症状积分以及血糖指标。结果治疗后,研究组总有效率高于对照组;治疗后与治疗前比较,两组血清总Ⅰ型胶原氨基端延长肽(peptide of type I collagen,PINP)、β胶原降解产物(β-collagen degradation product,β-CTX)、I型胶原C末端肽(C-terminal peptide of type I collagen,CTX-1)、空腹血糖(fasting blood glucose,FBG)、餐后2 h血糖(2 h postprandial blood glucose,2 h PG)、糖化血红蛋白(glycosylated hemoglobin,HbA1c)水平、视觉模拟评分法(visual analogue scale,VAS)评分及各项中医症状积分降低,两组间治疗后相比,研究组更低;治疗后与治疗前比较,两组血清碱性磷酸酶(alkaline phosphatase,ALP)、护骨素(osteoprotegerin,OPG)及腰椎、双髋部骨密度水平升高,两组间治疗后相比,研究组水平更高,数据存在较显著的差异(P<0.05)。结论健脾益气活血汤治疗T2DM合并骨质疏松患者有助于缓解其临床症状,优化骨代谢指标,增加腰椎、双髋部骨密度,减轻疼痛,改善血糖指标,疗效显著。
基金Supported by The National Natural Science Foundation of China, No.30371818Shanghai Rising-Star Program, No. 07QA14052Shanghai Leading Academic Discipline Project, Y0302 and Shanghai Educational Development Foundation, No. 2007CG56
文摘AIM： To evaluate the effect of Chinese traditional medicinal prescription, JIANPI HUOXUE decoction （JHD） on cytokine secretion pathway in rat liver induced by lipopolysaccharide （LPS）. METHODS： Twenty-four male SD rats were divided into normal group （n = 4）, model group （n = 10） and JHD group （n = 10） randomly. Rats in model group and JHD group were administrated with normal saline or JHD via gastrogavage respectively twice a day for 3 d. One hour after the last administration, rats were injected with LPS via tail vein, 50 μg/kg. Simultaneously, rats in normal group were injected with equivalent normal saline. After LPS stimulation for 1.5 h, serum and liver tissue were collected. Pathological change of liver tissues was observed through hematoxylineosin （H.E.） staining. Tumor necrosis factor alpha （TNF-α） in serum were assayed by enzyme linked immunosorbent assay （ELISA）. The protein expression of TNF-α, phosphorylated inhibit-κB （p-κB） and CD68 in liver were assayed by Western blot. The distribution of CD68 protein in liver was observed through immunohistochemical staining. The mRNA expression of TNF-α, interleukin-6 （IL-6）, CD14, toll-like receptor 2 （TLR2） and TLR4 in liver were assayed by real-time RT-PCR.RESULTS： Predominant microvesicular change, hepatocyte tumefaction and cytoplasm dilution were observed in liver tissues after LPS administration as well as obvious CD68 positive staining in hepatic sinusoidal. After LPS stimulation, serum TNF-α （31.35 ± 6.06 vs 12225.40 ± 9007.03, P 〈 0.05）, protein expression of CD68 （1.13 ± 0.49 vs 3.36 ±1.69, P 〈 0.05）, p-IκB （0.01 ±0.01 vs 2.07 ＋ 0.83, P 〈 0.01） and TNF-α （0.27 ± 0.13 vs 1.29 ± 0.37, P 〈 0.01） in liver and mRNA expression of TNF-α （1.96 ± 2.23 vs 21.45 ±6.00, P 〈 0.01）, IL-6 （4.80 ± 6.42 vs 193.50 ± 36.36, P 〈 0.01） and TLR2 （1.44 ± 0.62 vs 4.16 ± 0.08, P 〈 0.01） in liver were also increased significantly. These pathological changes were all improved in .1HD group. On the other hand, TLR4 mRNA （1.22 ± 0.30 vs 0.50 ± 0.15, P 〈 0.05） was down-regulated and CD14 mRNA increased but not significantly after LPS stimulation. CONCLUSION： JHD can inhibit cytokine secretion pathway induced by LPS in rat liver, which is probably associated with its regulation on CD68, p-IκB and endotoxin receptor TLR2.
