母马生长激素细胞和催乳激素细胞的形态计测学研究

利用免疫组织化学和形态计测学的方法 ,观察了 1 8匹成熟雌性蒙古马的脑垂体前叶生长激素细胞和催乳激素细胞的数量和面积 ,同时利用放射免疫分析方法检测了这两种激素的血浆水平。结果表明 ,每个马脑垂体前叶中 ,生长激素细胞的平均数量为 6 .42× 1 8,每个细胞的平均面积为 82 .40μm2 ;催乳激素细胞的平均数量为 6 .0 7× 1 0 8,每个细胞的平均面积为 47.31μm2 。生长激素的血浆含量平均为 2 .84ng/ m L,但个体差异较大 ,变异系数高达 78.5 % ,催乳激素的血浆含量平均为 7.2 6 ng/ m L。本研究结果揭示 :母马脑垂体生长激素细胞和催乳激素细胞的数量和面积并不是决定母马这两种激素血中浓度的唯一重要因素 ;生长激素血中浓度上的个体差异 。

公马生长激素细胞和催乳激素细胞的形态计测学研究

利用免疫组织化学和形态计测学的方法 ,观察了 2 0匹成熟雄性蒙古马的脑垂体前叶生长激素细胞和催乳激素细胞的数量和面积 ,同时利用放射免疫分析方法检测了这 2种激素的血浆水平。结果表明 ,生长激素细胞的数量为10 .10× 10 8个 ,细胞面积为 81.30 μm2 ;催乳激素细胞的数量为 3 .94× 10 8个 ,细胞面积为 46 .0 3μm2 。生长激素在血浆中含量为 10 .11ng/mL ,催乳激素为 1.6 4ng/mL。结果提示 :垂体激素血浆中含量与相关细胞的组织学特征有关 ,而生长激素血浆中含量的个体差异可能与其搏动性分泌形式有关。

比格犬脑垂体远侧部细胞的电镜观察

透射电镜观察表明,比格犬(Beagle)脑垂体远侧部实质中含有6种细胞,即生长激素细胞、催乳激素细胞、促甲状腺激素细胞、促性腺激素细胞、促肾上腺皮质激素细胞和滤泡—星形细胞,它们各有其超微结构特征。生长激素细胞呈圆形;胞核为圆形;分泌颗粒很多,圆形,直径约233～465nm。催乳激素细胞多呈不规则形;胞核为不规则形;分泌颗粒很多,圆形,直径约310～620nm。促甲状腺激素细胞呈圆形或卵圆形;胞核为圆形或卵圆形;分泌颗粒较少,直径约78～232nm。促肾上腺皮质激素细胞呈圆形或卵圆形;胞核为圆形或卵圆形;分泌颗粒较多,圆形,直径约155～310nm,分布于细胞周边。促性腺激素细胞呈圆形或椭圆形;胞核为圆形或椭圆形;分泌颗粒较少,呈圆形、卵圆形、杵状或不规则形,长径约155～434nm。滤泡一星形细胞呈星形或不规则形,有突起伸入相邻细胞之间;胞核为圆形或卵圆形;胞质内无分泌颗粒。

乳头溢液的治疗研究

乳头溢液是乳腺疾病中的一个常见的单独性症状,有棕黄色、棕褐色、黄色或黄绿色、乳白色、浆液性、鲜红血性之分别,有时与妇女月经期有关,常为患病妇女的主诉或乳腺疾病的早期甚至是唯一症状,多见于35岁以上妇女的非哺乳期的病理性溢液。

Detection of PRL-2 gene expression in hepatocellular carcinoma by real-time fluorescence quantitative PCR

Objective:To quantitatively detect the expression level of PRL-2 in primary hepatocellular carcinoma using real-time fluorescence quantitative PCR.Methods:Total RNA isolated from human HCC and liver tissue adjacent to the tumor was reversely transcribed into cDNA.Real-time fluorescence quantitative PCR(Q-PCR) method was used to analyze the expres-sion level of PRL-2 gene.Results:The Q-PCR method was performed successfully to precisely detect RNA level.PRL-2 was expressed in all portal vein tumor thrombosis(PVTT) and HCC,but only in some paratumor tissue.The highest expression level of PRL-2 gene was recorded in PVTT;meanwhile expression level of PRL-2 was higher than that in paratumor liver tis-sues and in HCC(P < 0.01),and it was higher in HCC than that in paratumor liver tissues.Conclusion:The Q-PCR may be the most precise method to quantitatively detect RNA level and can be used in gene expression changes.The PRL-2 gene has higher expression in PVTT than that in HCC and in paratumor liver tissue cells,indicating that it plays an important role in the development and metastasis of the HCC.

BMP-4 accelerates PRL secreting,cell proliferation and invasiveness in human prolactinoma

Objective： The aim of the study was to explore the bone morphogenetic protein 4 （BMP-4） how to regulate pro- lactin （PRL） secreting, cell proliferation and invasiveness in human prolactinorna. Methods： Ten patients who diagnosed as prolactinoma by clinical characteristics and pathology were divided into two groups, one was sensitive to Brornocriptine, the other was insensitive to Brornocriptine. Every case was conducted by primal cell culture then treated by different concentrations of BMP-4. The cell configuration was observed and PRL hormone was measured in different times. The expressions of BMP-4 rnRNA and BMP-4 in 37 cases of prolactinorna and 8 cases of normal pituitary tissues samples were detected by immunohistochemical and in situ hybridization technique, and the results were analyzed by statistic methods. Results： BMP-4 （5 ng/rnL） could accelerate the secreting of PRL in prolactinorna cell, and it could reach the greatest effect in the concentration of 20 ng/mL. BMP-4 could increase prolactinoma cell proliferation, but when the concentration was 50 ng/rnL, the BMP-4 effect of increasing secreting decreased. When 100 ng/mL, the cell began to die. The effects of the BMP-4 in sensitive group and insensitive group had no difference. The BMP-4 was highly expressed in the prolactinornas and was positively related with the invasiveness grades. Conclusion： BMP-4 have positive regulation in prolactinoma secreting, proliferation, invasiveness effects, BMP-4 probably has the important role in prolactinoma pathogenesis.

Decreased prolactin-inducible protein expression exhibits inhibitory effects on the metastatic potency of breast cancer cells

Objective:The aim of the research was to study the effects of prolactin-inducible protein(PIP) downregulation on metastatic abilities of human breast cancer MDA-MB-453 cells.Methods:PIP-siRNA was transfected into human breast cancer MDA-MB-453 cells through liposome.Reverse transcription PCR and immunocytochemistry were employed to detect the downregulated expression of PIP.Cell migration,adhesion and invasion assays were performed to assess the impacts of PIP downregulation on cell migration,adhesion and invasion respectively.Results:Knockdown of PIP obviously inhibited cell migration,the migrated cells were decreased by 83.1% compared with the negative control group.Cell adhesion was also reduced,the adhesion rates at 30 min and 60 min were decreased by 42.6% and 48.5% respectively compared with the negative control group.Moreover,PIP downregulation resulted in decreased invasion rate by 73.9%.Conclusion:Reduced PIP expression in MDA-MB-453 cells can inhibit the abilities of migration,adhesion and invasion,which suggests that PIP plays an important role in the metastatic potency of breast cancer cells.

Effects of prolactin on HLA-DR and CD40 expressions by human thyrocytes

Objective To study the effects of prolactin(PRL) on HLA-DR and CD40 expressions by human thyrocytes,and to investigate the possible mechanisms for PRL to affect the development of Graves'disease(GD).Methods Thyrocytes in secondary culture,which were from GD thyroid glands the tissues adjacent to the lwesions of multinodular goiter and andenoma(control group),were treated respectively with ovine PRL (oPRL),interferon-γ(IFN-γ),interlukin-4(IL-4)and oPRL plus IFN-γ(10U/ml)or IL-4(5ng/ml)for 7days.HLA-DR and CD40 expressions on the thyrocytes were determined by immunofluorescent staining and flow cytometry. Results oPRL(12.5ng/ml-1000ng/ml)had no significant direct effect on HLA-DR or CD40 expression.It did not significantly affect the stimulation of HLA-DR expressions on the rwo groups of thyrocyte treated with IFN-γ or on GD thyrocytes treated with IL-4.oPRL could antagonize the stimulation of CD40 expressions by IFN-γ and the inhibition by IL-4 on both groups of thyrocytes.The antagonizing effects were related to the concentration of PRL.IFN-γ-stimulated percentages of CD40+ thyrocytes and delta mean fluorescence intensity(dMF)in both thyrocyte sources were significantly reduced in the presence of 200ng/ml oPRL(both GD and Control:P<0.01and P<0.05,respectively;Control:P<0.05)and 1000ng/ml oPRL(GD:P<0.01;Control:P<0.05).oPRL caused significant increasing in IL-4-inhibited percentages of CD40+ cells from the two groups of thyrocytes at 12.5ng/ml and 1000ng/ml and dMF from GD thyrocytes at 1000ng/ml(P<0.05).Conclusions PRL can exert indirect effects on CD40 expressions on thyrocytes by antagonizing the modulatory actions of IFN-γand IL-4 with dose-related effects.This may be one important mechanism for PRL to affect the development of GD.