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Regulation of axonal regeneration following the central nervous system injury in adult mammalian 被引量:3
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作者 刘冉 陈溪萍 陶陆阳 《Neuroscience Bulletin》 SCIE CAS CSCD 2008年第6期395-400,共6页
It has been well established that the recovery ability of central nervous system (CNS) is very poor in adult mammals. As a result, CNS trauma generally leads to severe and persistent functional deficits. Thus, the i... It has been well established that the recovery ability of central nervous system (CNS) is very poor in adult mammals. As a result, CNS trauma generally leads to severe and persistent functional deficits. Thus, the investigation in this field becomes a "hot spot". Up to date, accumulating evidence supports the hypothesis that the failure of CNS neurons to regenerate is not due to their intrinsic inability to grow new axons, but due to their growth state and due to lack of a permissive growth environment. Therefore, any successful approaches to facilitate the regeneration of injured CNS axons will likely include multiple steps: keeping neurons alive in a certain growth-state, preventing the formation of a glial scar, overcoming inhibitory molecules present in the myelin debris, and giving direction to the growing axons. This brief review focused on the recent progress in the neuron regeneration of CNS in adult mammals. 展开更多
关键词 central nervous system ADULT REGENERATION REGULATION brain injury
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Multiple Node Upset in SEU Hardened Storage Cells 被引量:4
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作者 刘必慰 郝跃 陈书明 《Journal of Semiconductors》 EI CAS CSCD 北大核心 2008年第2期244-250,共7页
We study the problem of multiple node upset (MNU) using three-dimensional device simulation. The results show the transient floating node and charge lateral diffusion are the key reasons for MNU. We compare the MNU ... We study the problem of multiple node upset (MNU) using three-dimensional device simulation. The results show the transient floating node and charge lateral diffusion are the key reasons for MNU. We compare the MNU with multiple bit upset (MBU),and find that their characteristics are different. Methods to avoid MNU are also discussed. 展开更多
关键词 multiple node upset hardened cell charge collection
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Analysis of the Elastic-Plastic Dynamic Finite Element on a Jointed Rock Mass 被引量:1
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作者 郭文章 冯顺山 王海福 《Journal of Beijing Institute of Technology》 EI CAS 1999年第2期27-32,共6页
Aim To study the elastic plastic dynamical constitutive relations about a jointed rock mass under explosion load and its computer simulation. Methods\ Stress history is taken into account and stresses will follow ch... Aim To study the elastic plastic dynamical constitutive relations about a jointed rock mass under explosion load and its computer simulation. Methods\ Stress history is taken into account and stresses will follow changes in time during a period of explosion load. According to the principle of static force balance, the corresponding nodal concentrated force is calculated and the nodal displacement is counted. The elastic plastic dynamic finite element equations are thus obtained. Results\ A finite element method is given for a jointed rock mass under explosion load. Conclusion\ The problem of large plastic deformation for jointed rock mass on blasting was efficiently resolved through dynamic finite element analysis and the range of damages by blasting simulated, and this pushes forward the problem to engineering practice. 展开更多
关键词 jointed rock mass dynamic finite element numerical simulation
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Hybrid finite-discrete element modelling of asperity degradation and gouge grinding during direct shearing of rough rock joints 被引量:10
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作者 H. Y. Liu Haoyu Han +1 位作者 H. M. An J. J. Shi 《International Journal of Coal Science & Technology》 EI 2016年第3期295-310,共16页
A hybrid finite-discrete element method was implemented to study the fracture process of rough rock joints under direct shearing. The hybrid method reproduced the joint shear resistance evolution process from asperity... A hybrid finite-discrete element method was implemented to study the fracture process of rough rock joints under direct shearing. The hybrid method reproduced the joint shear resistance evolution process from asperity sliding to degradation and from gouge formation to grinding. It is found that, in the direct shear test of rough rock joints under constant normal displacement loading conditions, higher shearing rate promotes the asperity degradation but constraints the volume dilation, which then results in higher peak shear resistance, more gouge formation and grinding, and smoother new joint surfaces. Moreover, it is found that the joint roughness affects the joint shear resistance evolution through influencing the joint fracture micro mechanism. The asperity degradation and gouge grinding are the main failure micro-mechanism in shearing rougher rock joints with deeper asperities while the asperity sliding is the main failure micro-mechanism in shearing smoother rock joints with shallower asperities. It is concluded that the hybrid finite-discrete element method is a valuable numerical tool better than traditional finite element method and discrete element method for modelling the joint sliding, asperity degradation, gouge formation, and gouge grinding occurred in the direct shear tests of rough rock joints. 展开更多
关键词 Hybrid FEM-DEM Rock joint Asperity sliding Asperity shearing Fragment grinding
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The ultimate strength of doubler plate reinforced Y-joints under compression loading 被引量:1
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作者 FENQ Qi TAN Jia-hua 《Journal of Marine Science and Application》 2005年第2期13-19,共7页
It is common practice in the offshore industry to solve the punching shear problem due to compression by using doubler plate. The finite-element method is a useful tool for studying this problem. The aim of this paper... It is common practice in the offshore industry to solve the punching shear problem due to compression by using doubler plate. The finite-element method is a useful tool for studying this problem. The aim of this paper is to study the static strength of doubler plate reinforced Y-joints subjected to compression loading. The finite-element method is adopted in numerical parametric studies. The individual influences of the geometric parameters βand τd (doubler plate to chord wall thickness ratio) and ld/d1(dubler plate length to brace diameter ratio) on the ultimate strength are made clear. The results show the size of plate may have important effects on the strength of reinforced joints. It is found that the ultimate strength of Y-joints reinforced with appropriately proportioned doubler plates can be greatly improved nearly up tothree times to un-reinforced Y-joints. 展开更多
关键词 static strength finite element analysis doubler plates reinforced joints Y-joints
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Inhibitory effect of schisandrin B on free fatty acid-induced steatosis in L-02 cells 被引量:5
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作者 Jian-Hong Chu Hui wang +4 位作者 yan ye Ping-Kei Chan Si-Yuan Pan Wang-Fun Fong Zhi-Ling Yu 《World Journal of Gastroenterology》 SCIE CAS CSCD 2011年第19期2379-2388,共10页
AIM:To investigate the effects of schisandrin B (Sch B) on free fatty acid (FFA)-induced steatosis in L-02 cells.METHODS:Cellular steatosis was induced by incubating L-02 cells with a FFA mixture (oleate and palmitate... AIM:To investigate the effects of schisandrin B (Sch B) on free fatty acid (FFA)-induced steatosis in L-02 cells.METHODS:Cellular steatosis was induced by incubating L-02 cells with a FFA mixture (oleate and palmitate at the ratio of 2:1) for 24 h.Cytotoxicity and apoptosis were evaluated by 3-(4,5-dmethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay and Annexin V/propidium iodide staining,respectively.Cellular total lipid was determined using a photocolorimetric method after Nile red staining,and triglyceride content was measured using an enzymatic kit.To study the effects of Sch B on steatosis,L-02 cells were treated with Sch B (1-100 μmol/L) in the absence or presence of 1 mmol/L FFA for 24 h,and cellular total lipid and triglyceride levels were measured.To explore the mechanisms of action of Sch B in the steatotic L-02 cells,mRNA levels of several regulators of hepatic lipid metabolism including adipose differentiation related protein (ADRP),sterol regulatory element binding protein 1 (SREBP-1),peroxisome proliferator-activated receptor (PPAR)-α and PPAR-γ were measured by quantitative real-time polymerase chain reaction (PCR),and protein levels of ADRP and SREBP-1 were measured by immunoblotting.RESULTS:Treatment with 1 mmol/L FFA for 24 h induced intracellular lipid accumulation in L-02 cells comparable to that in human steatotic livers without causing apparent apoptosis and cytotoxicity.Sch B mitigated cellular total lipid and triglyceride accumulations in the steatotic L-02 cells in a dose-dependent manner.Quantitative real-time PCR and Western blot analyses revealed that treatment of L-02 cells with 100 μmol/L Sch B reverted the FFA-stimulated up-regulation of ADRP and SREBP-1.CONCLUSION:Sch B inhibits FFA-induced steatosis in L-02 cells by,at least in part,reversing the up-regulation of ADRP and SREBP-1. 展开更多
关键词 Free fatty acid Hepatic lipid metabolism Hepatocellular steatosis L-02 cells Schisandrin B
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ART-2 neural network based on eternal term memory vector:Architecture and algorithm
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作者 赵学智 叶邦彦 《Journal of Harbin Institute of Technology(New Series)》 EI CAS 2009年第6期843-848,共6页
Aimed at the problem that the traditional ART-2 neural network can not recognize a gradually changing course, an eternal term memory (ETM) vector is introduced into ART-2 to simulate the function of human brain, i.e. ... Aimed at the problem that the traditional ART-2 neural network can not recognize a gradually changing course, an eternal term memory (ETM) vector is introduced into ART-2 to simulate the function of human brain, i.e. the deep remembrance for the initial impression.. The eternal term memory vector is determined only by the initial vector that establishes category neuron node and is used to keep the remembrance for this vector for ever. Two times of vigilance algorithm are put forward, and the posterior input vector must first pass the first vigilance of this eternal term memory vector, only succeeded has it the qualification to begin the second vigilance of long term memory vector. The long term memory vector can be revised only when both of the vigilances are passed. Results of recognition examples show that the improved ART-2 overcomes the defect of traditional ART-2 and can recognize a gradually changing course effectively. 展开更多
关键词 ART-2 neural network eternal term memory vector two times of vigilance gradually changing course pattern recognition
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A simple and robust method for essential boundary condition treatments in mesh-less methods
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作者 赵伟民 郭峰 李瑰贤 《Journal of Harbin Institute of Technology(New Series)》 EI CAS 2009年第2期237-241,共5页
To improve the treatment efficiency of essential boundary condition in mesh-less methods, a simple and robust method is proposed in this paper. Rising weight of nodes in the construction of trail function, specified f... To improve the treatment efficiency of essential boundary condition in mesh-less methods, a simple and robust method is proposed in this paper. Rising weight of nodes in the construction of trail function, specified for essential boundary condition, can make the trail function pass through these nodes. And then, the trail function can satisfy the essential boundary condition previously by setting diagonal element to 1 or multiplying diagonal element by a big number in FEM. The MLS method is adopted to validate this method, and it is proved that this method is eostless and robust in most of mesh-less methods. 展开更多
关键词 mesh-less method essential boundary condition rising weight
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The Establishment and Development of Finite Element Model of Human Cervical Vertebra and Its Application Example 被引量:8
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作者 SHEN Xiao-wen YU Hang-ping ZOU Wei 《Chinese Journal of Biomedical Engineering(English Edition)》 2008年第2期86-92,共7页
The incidence rate of cervical spondylosis is high,and due to the complicacy of cervical vertebra structure,irregularity of shapes and non-uniformity of components,sometimes it’s difficult to achieve planned objectiv... The incidence rate of cervical spondylosis is high,and due to the complicacy of cervical vertebra structure,irregularity of shapes and non-uniformity of components,sometimes it’s difficult to achieve planned objectives by experiments in vitro through stress and strain analysis. Besides,the biomechanical factors are of vital significance in the cause of spinal disorders. In this paper the author makes a summary of the present modeling of human cervical vertebra and describes the major methods of establishing the finite element model of human cervical vertebra through several self-constructed models. With the advance of computer technology,the finite element methods have been rapidly developed in cervical vertebra biomechanical researches and have became a major approach for biomechanical researches to simulate more and more clinical conditions. 展开更多
关键词 cervical vertebra finite element model research
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Omega-3 fatty acids for the treatment of non-alcoholic fatty liver disease 被引量:5
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作者 Matteo Nicola Dario Di Minno Anna Russolillo +3 位作者 Roberta Lupoli Pasquale Ambrosino Alessandro Di Minno Giovanni Tarantino 《World Journal of Gastroenterology》 SCIE CAS CSCD 2012年第41期5839-5847,共9页
Non-alcoholic fatty liver disease (NAFLD) has been recognized as a major health burden. It is the most important cause of chronic liver disease and a major in- dependent cardiovascular risk factor. Lacking a definit... Non-alcoholic fatty liver disease (NAFLD) has been recognized as a major health burden. It is the most important cause of chronic liver disease and a major in- dependent cardiovascular risk factor. Lacking a definite treatment for NAFLD, a specific diet and an increase in physical activity represent the most commonly used therapeutic approaches. In this review, major literature data about the use of omega-3 polyunsaturated fatty ac- ids (n-3 PUFAs) as a potential treatment of NAFLD have been described, n-3 PUFAs, besides having a beneficial impact on most of the cardio-metabolic risk factors (hy- pertension, hyperlipidemia, endothelial dysfunction and atherosclerosis) by regulating gene transcription factors [i.e., peroxisome proliferator-activated receptor (PPAR) cz, PPARy, sterol regulatory element-binding protein-i, carbohydrate responsive element-binding protein], im- pacts both lipid metabolism and on insulin sensitivity. In addition to an enhancement of hepatic beta oxidation and a decrease of the endogenous lipid production, n-3 PUFAs are able to determine a significant reduction of the expression of pro-inflammatory molecules (tumor necrosis factor-~ and interleukin-6) and of oxygen reac- tive species. Further strengthening the results of the in vitro studies, both animal models and human interven- tion trials, showed a beneficial effect of n-3 PUFAs on the severity of NAFLD as expressed by laboratory pa- rameters and imaging measurements. Despite available results provided encouraging data about the efficacy of n-3 PUFAs as a treatment of NAFLD in humans, well- designed randomized controlled trials of adequate size and duration, with histological endpoints, are needed to assess the long-term safety and efficacy of PUFA, as well as other therapies, for the treatment of NAFLD and non-alcoholic steatohepatitis patients. It is worthwhile to consider that n-3 PUFAs cannot be synthesized by the human body and must be derived from exogenous sources (fish oil, flaxseeds, olive oil) which are typical foods of the Mediterranean diet, known for its beneficial effects in preventing obesity, diabetes and, in turn, cardiovascular events. According to these data, it is important to consider that most of the beneficial effects of n-3 PUFAs can also be obtained by an equilibrate nutrition program. 展开更多
关键词 Hepatic steatosis Non-alcoholic fatty liverdisease Omega-3 polyunsaturated fatty acids Animalmodels
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Identification of a NF-кB site in the negative regulatory element (ε-NRAII) of human ε-globin gene and its binding protein NF-кB p50 in the nuclei of K562 cells
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作者 CHUN Hui HOU, JIAN HUANG, Ruo LAN QIAN Group of Globin Gene Expression and Regulation, State Key Labortory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 《Cell Research》 SCIE CAS CSCD 2002年第1期79-82,共4页
The developmental control of the human e-globin gene expression is mediated by transcription regulatory elements in the 5’ flanking DNA of this gene. Sequence analysis has revealed a DNA motif (GGGGAATTTGCT) similar ... The developmental control of the human e-globin gene expression is mediated by transcription regulatory elements in the 5’ flanking DNA of this gene. Sequence analysis has revealed a DNA motif (GGGGAATTTGCT) similar to NF-кB consensus sequence resides in the negative regulatory element (-3028bp~ -2902bp, termed ε-NRAII) 5’ to the cap site of this gene. NRF DNA fragment (-3010bp~ -2986bp) containing the NF-кB motif similar sequence was synthesized and used in electrophoresis mobility shift assay (EMSA) and competitive analysis. Data showed that a protein factor from nuclear extracts of K562 cells specifically interacted with NRF DNA fragment. The synthetic NF DNA fragment (containing NF-кB consensus sequence) could competed for the protein binding, but MNF DNA fragment (mutated NF-кB motif) could not, suggesting that the binding protein is a member of NF-кB/Rel family. Western blot assay demonstrated that the molecular weight of NF-кB protein in the nuclei of K562 cells is 50ku. We suggested that NF-кB p50 may play an important role in the regulation of human c-globin gene expression. 展开更多
关键词 Human ε-globin gene negative regulatory element NE-кB p50.
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RAT EXOCRINE PANCREATIC SECRETION BYVAGAL STIMULATION OCCURSVIAMULTIPLEMEDIATORS
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作者 何晓东 《Chinese Medical Sciences Journal》 CAS CSCD 1996年第2期73-77,共5页
The vagus is a mixed nerve containing cholinerrgic and non-cholinergic neurons. Vagal fibers interact with peptidergic neurons of the enteric nervous system which stain immunohistochemically for cholecystokinin, vasoa... The vagus is a mixed nerve containing cholinerrgic and non-cholinergic neurons. Vagal fibers interact with peptidergic neurons of the enteric nervous system which stain immunohistochemically for cholecystokinin, vasoactive intestinal polypeptide, and gastrin releasing peptide. The contribution of these peptidergicneurons in the pancreatic response to vagal stimulation is unknown. We tested the effect of specific inhibitor of these stimulants against vagally mediated exocrine secretion in rats. The response to vagal stimulation was blocked significantly by each of the following:the ganglionic blocker hexamethonium (l00% inhibition); the muscarinic, cholinergic blocker atropine (85% inhibition); the specific cholecystokinin-A receptor blocker (91 % inhibition); and a vasoactive intestinal polypeptide polyclonal antibody (89% inhibition). This observation is consistent with the hypothesis that potentiating interactions among several agonists mediate the vagal response. Our study, however, dose not exclude acetylcholine as the final commommediator. 展开更多
关键词 CHOLECYSTOKININ gastrin-releasing peptide: dimethyl sulfoxide
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Prokaryotic expression of recombinant human p75NTR-Fc fusion protein and its effect on the neurite outgrowth of dorsal root ganglia neuron 被引量:1
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作者 Zhu Feng Wang Yongtang +2 位作者 Lu Xiumin Zeng Lin Wu Yamin 《Journal of Medical Colleges of PLA(China)》 CAS 2009年第1期1-9,共9页
Objective: To clone, express, and identify the extracellular domain gene of human p75 neurotrophin receptor with IgG-Fe (hp75NTR-Fc) in prokaryotic expression system, and investigate the effect of the recombinant p... Objective: To clone, express, and identify the extracellular domain gene of human p75 neurotrophin receptor with IgG-Fe (hp75NTR-Fc) in prokaryotic expression system, and investigate the effect of the recombinant protein on dorsal root ganglia (DRG) neuron neurites. Methods: The hp75NTR-Fc coding sequence was amplified from pcDNA-hp75NTR-Fc by polymerase chain reaction (PCR) and subcloned into vector pET30a (+), in which hp75NTR-Fc expression was controlled under the T7 promoter. The recombinant vectors were amplified in E. coli DH5α and identified by PCR, enzyme digestion and sequencing, and then transformed into E. coli BL21 (DE3). The expression product was analyzed with SDS-PAGE and Western blot. Then after the recombinant protein purified with Protein A affinity chromatograph, and renaturated with dialysis, respectively, the effect of the recombinant protein on DRG neuron neuritis was further investigated. Results: The results of PCR, enzyme digestion, and sequencing demonstrated the success of inserting the hp75NTR-Fc fragment into vector pET30a (+). SDS-PAGE and Western blot showed a positive protein band with molecular weight about 50 kD in the expression product, which is accordant with the interest protein, and this band could be specifically recognized by rabbit anti-NGFRp75 antibody. The purified infusion protein following dialysis could promote neurite outgrowth of DRG neurons cultured with myelin-associated glycoprotein (MAG). Conclusion: The hp75NTR-Fc coding sequence was subcloned into the expression vector pET30a (+) correctly and expressed successfully in the prokaryotie expression system. The infusion protein could promote neurite outgrowth of DRG neurons cultured with MAG. 展开更多
关键词 P75NTR Vector construction Prokaryotic expression Dorsal root ganglia neuron Neurite outgrowth
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Link-Vertex Topology Aggregation for Multi-domain Path Computation with Hierarchical PCE Architecture 被引量:1
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作者 Peng Yunfeng Chen Zhen +2 位作者 Zhao Changming Wang Zongwei Long Keping 《China Communications》 SCIE CSCD 2012年第1期86-91,共6页
Inter-domain path computing is one big issue in multi-domain networks. The Hierarchical Path Computing Element (H-PCE) is a semi-central architecture for computing inter-domain path. To facilitate H-PCE in inter-domai... Inter-domain path computing is one big issue in multi-domain networks. The Hierarchical Path Computing Element (H-PCE) is a semi-central architecture for computing inter-domain path. To facilitate H-PCE in inter-domain path computing, this paper proposed a topology aggregation scheme to abstract the edge nodes and their connected inter-domain link as one vertex to achieve more optimal paths and confidentiality guarantee. The effectiveness of the scheme has been demonstrated on solving wavelength routing in multi-domain Wavelength Division Multiplexing (WDM) network via simulation. Simulation results show that this scheme reduces at least 10% inter-domain blocking probability, compared with the traditional Domain-to-the-Node (DtN) scheme. 展开更多
关键词 ROUTING PCE link-vertex aggregation inter-domain path computation multi-domain network topology aggregation
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Neural adaptive PSD decoupling controller and its application in three-phase electrode adjusting system of submerged arc furnace 被引量:4
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作者 贺建军 刘郁乔 +1 位作者 喻寿益 桂卫华 《Journal of Central South University》 SCIE EI CAS 2013年第2期405-412,共8页
Taking three-phase electrode adjusting system of submerged arc furnace as study object which has nonlinear, time-variant, multivariable and strong coupling features, a neural adaptive PSD(proportion, sum and different... Taking three-phase electrode adjusting system of submerged arc furnace as study object which has nonlinear, time-variant, multivariable and strong coupling features, a neural adaptive PSD(proportion, sum and differential) dispersive decoupling controller was developed by combining neural adaptive PSD algorithm with dispersive decoupling network. In this work, the production technology process and control difficulties of submerged arc furnace were simply introduced, the necessity of establishing a neural adaptive PSD dispersive decoupling controller was discussed, the design method and the implementation steps of the controller are expounded in detail, and the block diagram of the controlled system is presented. By comparison with experimental results of the conventional PID controller and the adaptive PSD controller, the decoupling ability, adaptive ability, self-learning ability and robustness of the neural adaptive PSD dispersive decoupling controller have been testified effectively. The controller is applicable to the three-phase electrode adjusting system of submerged arc furnace, and it will play an important role for achieving the power balance of three-phrase electrodes, saving energy and reducing consumption in the process of smelting. 展开更多
关键词 PSD algorithm decoupling controller submerged arc furnace three phase electrode
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Energy conserving effects of dividing wall column 被引量:4
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作者 方静 赵寒梅 +3 位作者 祁建超 李春利 齐俊杰 郭佳佳 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2015年第6期934-940,共7页
The energy-conserving performance of dividing wall column(DWC) is discussed in this paper. The heat transfer through the dividing wall is considered and the results are compared with that of common heat insulation div... The energy-conserving performance of dividing wall column(DWC) is discussed in this paper. The heat transfer through the dividing wall is considered and the results are compared with that of common heat insulation dividing wall column(HIDWC). Based on the thermodynamic analysis of heat transfer dividing wall column(HTDWC) and HIDWC, both computer simulation and experiments are employed to analyze the energyconserving situation. Mixtures of n-hexane, n-heptane and n-octane are chosen as the example for separation.The results show that the energy consumption of HTDWC is 50.3% less than that of conventional distillation column, while it is 46.4% less than that of HIDWC. It indicates that DWC is efficient on separating threecomponent mixtures and HTDWC can save more energy than HIDWC. Thus it is necessary to consider the heat transfer while applying DWC to industry. 展开更多
关键词 Dividing wall column ENERGY-SAVING Heat transfer Back-mixing Thermodynamic efficiency
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Double Ninth Festival:Respect for Elders and Prayers for Blessings
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《China Today》 2016年第10期78-80,共3页
THE Double Ninth Festival falls on the ninth day of the ninth lunar month.Chinese ancients believed that nine was the largest number.According to the yin/yang dichotomy that forms the basis of the Chinese view of the ... THE Double Ninth Festival falls on the ninth day of the ninth lunar month.Chinese ancients believed that nine was the largest number.According to the yin/yang dichotomy that forms the basis of the Chinese view of the world,yin represents the elements of darkness and yang represents life and brightness.The number nine is regarded as yang. 展开更多
关键词 lunar brightness Respect autumn festival custom heritage mountains winter listed
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The Expression of Ca N and CaMK is Associated with Lipogenesis in the Muscle of Chicken
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作者 Ye YANG Jiao SONG +2 位作者 Ruiqi FUI Yanfa SUN Jie WEN 《Agricultural Science & Technology》 CAS 2015年第4期761-764,共4页
Intramuscular fat (IMF) content in chickens significantly contributes to meat quality. The main objective of this study was to assess the expression of calcineudn (CAN) and Ca^2+/calmodutin-dependent protein kina... Intramuscular fat (IMF) content in chickens significantly contributes to meat quality. The main objective of this study was to assess the expression of calcineudn (CAN) and Ca^2+/calmodutin-dependent protein kinase (CAME) in lipogene- sis in chicken muscle. The chickens were slaughtered and sampled at the ages of 4, 8, and 16 weeks, respectively. IMF content and the expression of CaN subunits and CaMK isoforms were measured in thigh muscle tissue. The results showed that the IMF contents were higher in chickens at the age of 16 weeks compared with those in chickens at the ages of 4 and 8 weeks (P〈0.05). The expression levels of fatty acid synthase (FAS) and fatty acid translocase CD36 (FAT/CD36) mRNA in 16-week-old chickens were all significantly up-regulated compared with those in 4-week-old chickens (P〈0.05). The mRNA levels of CaNB and CaMK IV in 16-week-old chickens were significantly lower than those in 4-week-old chickens (P〈0.05). But the CaMK II mRNA levels in 16-week-old chickens were significantly higher than those in 4-week-old chickens (P〈0.05). To investigate the roles of CaMK and CaN in adipogenesis, SV cells were incubated in standard adipogenesis medium for 24 h and treated with specific inhibitor of CaMK and CaN. The ex- pressions of CCAAT/enhancer binding protein β(C/EBPJ3), sterol regulatory element- binding protein 1 (SREBP1) and peroxisome proliferation-activated receptor ), (PPARy) were dramatically enhanced by CsA and CaN inhibitor (P〈0.05). KN93, a CaMK Ⅱ inhibitor, dramatically repressed the expression of those lipogenic genes (P〈0.05). All the results above indicated that CaN and CaMK had different effects on adipogenesis in the muscle of chickens. 展开更多
关键词 Ca^2+/calmodulin-dependent protein kinase CALCINEURIN CHICKEN Regula-tion
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Gene expression profiling and endothelin in acute experimental pancreatitis 被引量:6
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作者 Helieh S Oz Ying Lu +3 位作者 Louis P Vera-Portocarrero Pei Ge Ada Silos-Santiago Karin N Westlund 《World Journal of Gastroenterology》 SCIE CAS CSCD 2012年第32期4257-4269,共13页
AIM:To analyze gene expression profiles in an experimental pancreatitis and provide functional reversal of hypersensitivity with candidate gene endothelin-1 antagonists.METHODS:Dibutyltin dichloride(DBTC) is a chemica... AIM:To analyze gene expression profiles in an experimental pancreatitis and provide functional reversal of hypersensitivity with candidate gene endothelin-1 antagonists.METHODS:Dibutyltin dichloride(DBTC) is a chemical used as a polyvinyl carbonate stabilizer/catalyzer,biocide in agriculture,antifouling agent in paint and fabric.DBTC induces an acute pancreatitis flare through generation of reactive oxygen species.Lewis-inbred rats received a single i.v.injection with either DBTC or vehicle.Spinal cord and dorsal root ganglia(DRG) were taken at the peak of inflammation and processed for transcriptional profiling with a cDNA microarray biased for rat brain-specific genes.In a second study,groups of animals with DBTC-induced pancreatitis were treated with endothelin(ET) receptor antagonists [ET-A(BQ123) and ET-B BQ788)].Spontaneous pain related mechanical and thermal hypersensitivity were measured.Immunohistochemical analysis was performed using anti-ET-A and ET-B antibodies on sections from pancreatic tissues and DRG of the T10-12 spinal segments.RESULTS:Animals developed acute pancreatic inflammation persisting 7-10 d as confirmed by pathological studies(edema in parenchyma,loss of pancreatic architecture and islets,infiltration of inflammatory cells,neutrophil and mononuclear cells,degeneration,vacuolization and necrosis of acinar cells) and the painrelated behaviors(cutaneous secondary mechanical and thermal hypersensitivity).Gene expression profile was different in the spinal cord from animals with pancreatitis compared to the vehicle control group.Over 260 up-regulated and 60 down-regulated unique genes could be classified into 8 functional gene families:circulatory/acute phase/immunomodulatory;extracellular matrix;structural;channel/receptor/transporter;signaling transduction;transcription/translation-related;antioxidants/chaperones/heat shock;pancreatic and other enzymes.ET-1 was among the 52 candidate genes upregulated greater than 2-fold in animals with pancreatic inflammation and visceral pain-related behavior.Treatments with the ET-A(BQ123) and ET-B(BQ-788) antagonists revealed significant protection against inflammatory pain related mechanical and thermal hypersensitivity behaviors in animals with pancreatitis(P < 0.05).Open field spontaneous behavioral activity(at baseline,day 6 and 30 min after drug treatments(BQ123,BQ788) showed overall stable activity levels indicating that the drugs produced no undesirable effects on normal exploratory behaviors,except for a trend toward reduction of the active time and increase in resting time at the highest dose(300 μmol/L).Immunocytochemical localization revealed that expression of ET-A and ET-B receptors increased in DRG from animals with pancreatitis.Endothelin receptor localization was combined in dual staining with neuronal marker NeuN,and glia marker,glial fibrillary acidic protein.ET-A was expressed in the cell bodies and occasional nuclei of DRG neurons in na ve animals.However,phenotypic expression of ET-A receptor was greatly increased in neurons of all sizes in animals with pancreatitis.Similarly,ET-B receptor was localized in neurons and in the satellite glia,as well as in the Schwann cell glial myelin sheaths surrounding the axons passing through the DRG.CONCLUSION:Endothelin-receptor antagonists protect against inflammatory pain responses without interfering with normal exploratory behaviors.Candidate genes can serve as future biomarkers for diagnosis and/or targeted gene therapy. 展开更多
关键词 Gene expression Endothelin receptors Pancreatitis Pain Dibutyltin dichloride Hypersensitivity Hyperalgesia
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Effects of Lanthanum-Amino Acid Complexes on Egg Hatching and Nauplius Metamorphosis of Penaeus chinensis
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作者 辛福言 曲克明 《Marine Science Bulletin》 CAS 2002年第1期93-97,共5页
Studies have been carried out on the effect of lanthanum-amino acid complexes on embryo development and nauplius growth ofPenaeus chinensis. The experimental results indicate that: (1)The optimum concentrations of lan... Studies have been carried out on the effect of lanthanum-amino acid complexes on embryo development and nauplius growth ofPenaeus chinensis. The experimental results indicate that: (1)The optimum concentrations of lanthanum-proline and Lanthanumphenylalanine for the development of eggs in monocell and dicell stages are 1.50~ 4.00mg/L and 0.50~3.00 mg/L, respectively, the egg hatching rates being raised by 21.0 ~ 46.0% and 23.0 ~42.8% ( P < 0.05 ) respectively. (2)The optimum concentrations of lanthanum- proline complex and Lanthanum-phenylalanine complex for the growth ofnauplii are 1.50~4.00 mg/L and 0.50~3.00 mg/L, the metamorphosis rate from nauplius to protozoea being raised by 16.4 ~27.5% and 20.4~26.7% (P < 0.05 ) respectively. (3)The positive effect of lanthanum-amino acid complexes on egg hatching and nauplius metamorphosis of Penaeus chinensis is better than that of lanthanum. With regard to the positive effect, lanthanum-proline complex is better than Lanthanum-phenylalanine complex. 展开更多
关键词 Lanthanum-amino acid complex Penaeus chinensis Egg hatching NAUPLII
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