元勰、元顺《蝇赋》的创作与传播——兼论北魏中后期贵族咏物赋对《诗经》传统的继承

北魏中后期出现的元勰《蝇赋》与元顺《蝇赋》,皆系咏物抒愤之作。蝇作为谗佞小人的指向意义可追溯至《诗经》。元勰与元顺之赋,以蝇为题材,是北魏贵族自觉汉化,继承《诗经》传统的结果。北魏皇室贵族文学素养的提升,通过雅集、知赏与交游等方式体现,是此时期北魏文坛欣荣之景的缩影。

Expression Activity of CLCuV Bidirectional Promoter in Agrobacterium tumefaciens

Cotton leaf curl virus (CLCuV) belongs to the subgroup III of geminiviruses with single strand DNA genome. Study demonstrated that the bidirectional promoter of CLCuV had activity in Agrobacterium tumefaciens (Smith et Townsend) Conn. This is the first report for the activity of the bidirectional promoter of geminivirus in A. tumefaciens. Results showed that the activity of the complementary sense promoter was stronger than that of virion sense promoter, and was detected 2-fold higher than that of CaMV 35S promoter in A. tumefaciens. Moreover, the promoter 5' deletion analysis indicated that the mean GUS activity driven by a 287 nucleotides complementary sense promoter fragment (from-287 to the translation initiation site) is 4 times higher than that driven by the whole complementary sense promoter in A. tumefaciens. This result suggested that there might exist negative regulatory elements in this deleted fragment. The function of other cis-elements included in CLCuV complementary sense promoter was also discussed in this paper.

Three 5’-flanking Regions of crtO Encoding β-carotene Oxygenase in Haematococcus pluvialis

Three separate 5＇-flanking regions （1.1 kb, 1.9 kb and 2.2 kb） of crtO were cloned through walking upstream. Results of sequence analysis show that three 5＇-flanking regions of crtO might have some similar putative cis-acting elements such as ABA （abscisic acid）-responsive element （ABRE）, C-repeat/dehydration responsive element （C-repeat/DRE）, light-responsive element （G-box, GAG-motif, I-box and ATC-motif）, wound-responsive element （WUN-motif）, auxin-responsive element （TGA-element）, MeJA-responsive element （TGACG-element） and MYB binding site （MBS）, except for typical TATA box or CCAAT box. These findings might mean diversiform regulatory patterns of crtO being in astaxanthin biosynthesis of Haematococcus pluvialis.

Regulatory Analysis of IPP Isomerase Gene in Haematococcus pluvialis

The unicellular green alga Haematococcus pluvia/is uniquely accumulates carotenoids in the cytoplasm and in late developmental stages turns deep-red in color because of accumulation of astaxanthin in the cytosol. The enzyme, isopentenyl pyrophosphate （IPP） isomerase, plays a key role in astaxanthin biosynthesis of H. pluvialis. In this paper, two separate 5＇-flanking regions （1.8 kb and 2.5 kb） of IPP isomerase gene was cloned through walking upstream firstly. Results of sequence analysis =showed that two separate 5＇-flanking regions of IPP isomerase gene might have similar putative cis-acting elements such as ABA （abscisic acid）-responsive element （ABRE）, drought-responsive element （DRE/C-repeat）, light-responsive element （G-box, GAG-motif, I-box and ATC-motif）, heat-shock element （HSE）, wound-responsive element （WUN-motif）, SA （salicylic acid）-responsive element （TCA-element）, auxin-responsive element （TGA-element）, MeJA （methyl jasmonate）-responsive element （TGACG-element）, enhancer-like element involved in anoxic specific inducibility （GC-motif） and MYB binding sites （MBS and MRE）, except for typical TATA box or CCAAT box, which exhibit diversiform transcriptional patterns of IPP isomerase gene in astaxanthin biosynthesis of Haematococcus pluvialis.

The Development of Dynamic Compliant Wrist for Robots

Based on an analysis of the relative shaft-to-hole position and attiude errors, as well as of the mechanics and Kinematics in the process of automatic assembly of industrial robots, the paper studies the principle of construction of dynamic wrists. Type I-3 and Ⅱ-6 dynamic compliant wrists have been designed and made. Prblems in the production of compliant elements and the connection between compliant elements and wrists were also solved. A study on the results of tests of the function of two kinds of dynamic compliant wrists shows that the dynamic compliant wrist's compliancy function can be improved by adding metallic materials having higher longitudinal and transverse rigidity into the softer elstomer. And the design Principle is proved to be feasible and practicable. It can be expected that the use of dynamic compliant wrist will greatly lower the technical requirements of the shaft-hole assembly and the requirements in the resetting accuracy.

Deletion of 93 bp Far-upstream Fragment of Rice Cytosolic Fructose- 1, 6-Bisphosphatase Promoter Completely Alter Its Expression Pattern

The 1 195 bp 5′ flanking region of rice ( Oryza sativa L.) cytosolic fructose_1, 6_bisphosphatase (cyFBPase) can direct tissue, cell specific expression in transgenic rice. In order to identify sequence elements responsible for the regulation of mesophyll_specific expression, the 5′ flanking regions of -1 195 bp, -1 102 bp, -768 bp, and -644 bp upstream of the translation initiation ATG codon were fused to the reporter gene encoding β_glucuronidase (GUS) and transferred to rice via particle bombardment. Analysis of the 5′ promoter deletions identified that a 93 bp fragment between -1 195 bp and -1 102 bp is essential for directing mesophyll specific expression. High constitutive expression of GUS reporter gene was found in the -768 deletion lines and another two deletion series. These results indicate the great potential utility of the promoter in rice biotechnology.

Ni-loaded Catalyst Containing Activated Clay for Maleic Anhydride Hydrogenation to Succinic Anhydride

In this work, the hydrogenation of maleic anhydride to succinic anhydride in the presence of 5 m%Ni/clay catalysts was investigated. These catalysts were characterized by X-ray diffraction （XRD）, H2 temperature programmed reduction （TPR） and thermogravimetric analysis （TGA） techniques. The XRD and TPR studies showed that Ni was present as Ni2＋ on the support, which indicated that there were no elemental nickel （Ni^0） and Ni203 species in the unreduced samples. Increasing of calcination temperature to 650 ℃ leads to destruction of the support structure observed in TGA, while the catalyst sample calcined at 550 ℃ exhibits better performances than other samples. The ideal conversion of maleic anhydride （97.14%） and selectivity of succinic anhydride （99.55%） were realized at a reaction temperature of 180 ℃ and a weight hourly space velocity of 4 h^-1 under a reaction pressure of 1 MPa.

SPATIAL-TEMPORAL VARIATION OF HEAVY METAL ELEMENTS CONTENT IN COVERING SOIL OF RECLAMATION AREA IN FUSHUN COAL MINE

Grid method is employed for sampling covering soil at the test field,whic h is reclamation area filled by coal mining wastes for cropland in th e Fushun coal mine,Liaoning Province,the Northeast China.The soil samp les are taken at different locations,inclu ding three kinds of covering soil,th ree different depths of soil layers a nd four different covering ages of covering soil.The s patial-temporal variation of heavy metal element content in reclamatio n soil is stud-ied.The results indicate that the co ntent of heavy metal elements is decreasing year after year;the determin ant reason why the content of heavy metal elemen ts at 60cm depth layer is higher than t hat at 30cm depth layer and surface is fertiliz-er and manure application;the metal elements mainly come from external environment;there is no metal pollut ion coming from mother material(coal mining wastes)in plough layer of covering soil.

Solid-Liquid Equilibria of Several Binary and Ternary Systems Containing Maleic Anhydride

Solid-liquid equilibria (SLE) of three binary systems and seventernary systems containing maleic an- hydride (MA) are measured byvisual method. The experimental data are compared with the calculatedones with modified universal quasichemical functional group activitycoefficient (UNIFAC) method in which the interaction parametersbetween groups come from two sources, dortmund data bank (DDB), ifthere's any, and correlations based on our former presentedexperimental SLE data of twenty binary systems.

Genome-wide Identification and Analysis of DNA Methyltransferases in Grape

DNA methylation is an important epigenetic regulation mechanism, which is catalyzed by DNA methyltransferases. In this study, eight DNA methyltransferase genes were identified in grape genome to analyze the selective pressure, gene expression and codon usage bias. The results showed grape DNA methyltransferase MET subfamily underwent relatively strong purifying selection during evolution, while chromomethylase CMT subfamily underwent positive selection during evolution. Under different abiotic(heat, drought or cold) stresses, the expression level of many grape DNA methyltransferase genes changed significantly. The expression level of these genes might be related with cis-regulatory elements of their promoters. The results of codon usage bias analysis showed that synonymous codon bias existed in grape DNA methyltransferase gene family, which might be affected by mutation pressure. These results laid a solid foundation for in-depth study of DNA methyltransferases in grape.

最后一股顽匪就歼记

1955年7月17日凌晨,光泽县华桥乡官屯村的一座名叫黄狗窠的深山里,响起一阵枪声.匪穴茅棚中,三名顽匪尸横血污.人们欢呼着:"土匪打死了!土匪打死了!"就歼的是惊动中央并通令围剿的全省最后一股顽匪.

“天高皇帝远”指的是哪个皇帝

“天高皇帝远”是一句流传甚广的民间俗语，出自明朝时黄溥编订的《闲中今古录摘抄》。

Analysis of promoters of microRNAs from a Glycine max degradome library

Objective： MicroRNAs （miRNAs） are genome-encoded, small non-coding RNAs that play important functions in development, biotic and abiotic stress responses, and other processes. Our aim was to explore the regu- lation of miRNA expression. Methods： We used bioinformatics methods to predict the core promoters of 440 miRNAs identified from a soybean （Glycine max） degradome library and to analyze c/s-acting elements for 369 miRNAs. Results： The prediction results showed that 83.86% of the 440 miRNAs contained promoters in their upstream se- quences, and 8.64% （38 loci） in their downstream sequences. The distributions of two core promoter elements, TATA-boxes and transcription start sites （TSSs）, were similar. The cis-acUng elements were examined to provide clues to the function and regulation of spatiotemporal expression of the miRNAs. Analyses of miRNA cis-elements and targets indicated a potential auxin response factor （ARF）- and gibberellin response factor （GARF）-mediated negative feedback loop for miRNA expression. Conclusions： The features of miRNAs from a Glycine max degradome library obtained here provide insights into the transcription regulation and functions of miRNAs in soybean.

Synthetic promoters consisting of defined cis-acting elements link multiple signaling pathways to probenazole-inducible system

Probenazole （3-allyloxy-l,2-benzisothiazole-1,1-dioxide, PBZ）, the active component of Oryzemate, could induce systemic acquired resistance （SAR） in plants through the induction of salicylic acid （SA） biosynthesis. As a widely used chemical inducer, PBZ is a good prospect for establishing a new chemical-inducible system. We first designed artificially synthetic promoters with tandem copies of a single type of cis-element （SARE, JERE, GCC, GST1, HSRE, and W-box） that could mediate the expression of the tS-glucuronidase （GUS） reporter gene in plants upon PBZ treatment. Then we combined different types of elements in order to improve inducibility in the PBZ-inducible system. On the other hand, we were surprised to find that the cis-elements, which are responsive to jasmonic acid （JA） and ethylene, also responded to PBZ, implying that SA, JA, and ethylene pathways also would play important roles in PBZ＇s action. Further analysis demonstrated that PBZ also induced early events of innate immunity via a signaling pathway in which Ca2＋ influx and mitogen-activated protein kinase （MAPK） activity were involved. We constructed synthesized artificial promoters to establish a PBZ chemical-inducible system, and preliminarily explored SA, JA, ethylene, calcium, and MAPK signaling pathways via PBZ-inducible system, which could provide an insight for in-depth study.