Objectives: To clone genes specifically expressed in the placenta of patients with preeclampsia. and to explain the mechanism in the etiopathology of preeclampsia. Methods: The placentae of preeclamptic and normotensi...Objectives: To clone genes specifically expressed in the placenta of patients with preeclampsia. and to explain the mechanism in the etiopathology of preeclampsia. Methods: The placentae of preeclamptic and normotensive subjects with pregnancy were used as models, and the eDNA Library was constructed and 20 differentially expressed fragments were cloned after a new version of PCR-based subtractive hybridization. The false positive clones were identified by reverse dot blot analysis. With one of the obtained gene taken as the probe, the placentas of 10 normal pregnant women and 10 preeclamptic patients were studied by using dot hybridization methods. Results: Six false positive clones were identified by reverse dot blot, and the rest 14 clones were identified as preeclampsia-related genes. These clones were sequenced, and analyzed with BLAST analysis system. Eleven of 14 clones were genes already known, among which one belongs to necdin family; the rest 3 were identified as novel genes. These 3 genes were acknowledged by GenBank, with the accession numbers AF2322 16, AF2322 17, AF233648. The results of dot hybridization using necdin gene as probe were as follows: (1) There was this mRNA in the placental tissues of normal pregnancy as well as in that of preeclampsia. (2) The intensity of transcription of this mRNA in the placental tissues of preeclampsia increased significantly compared with that of the normal pregnancy (P＜0.05). Conclusions: This study for the first time reported this group of genes, especially necdin-expressing gene, which are related to the etiopathology of preeclampsia. In addition, the overtranscription of necdin gene has been found in preeclampsia. it is helpful in further studies of the etiology of preeclampsia.展开更多
The dividing wall column （DWC） is considered as a major breakthrough in distillation technology and has good prospect of industrialization. Model predictive control （MPC） is an advanced control strategy that has a...The dividing wall column （DWC） is considered as a major breakthrough in distillation technology and has good prospect of industrialization. Model predictive control （MPC） is an advanced control strategy that has acquired extensive applications in various industries. In this study, MPC is applied to the process for separating ethanol, n-propanol, and n-butanol ternary mixture in a fully thermally coupled DWC. Both composition control and tem- perature inferent/al control are considered. The multiobjective genetic algor/thm function ＂gamult/obj＂ in Matlab is used for the weight tuning of MPC. Comparisons are made between the control performances of MPC and PI strategies. Simulation results show that although both MPC and PI schemes can stabilize the DWC in case of feed disturbances, MPC generally behaves better than the PI strategy for both composition control and tempera- ture inferential control, resulting in a more stable and superior performance with lower values of integral of squared error （ISE）.展开更多
AIM： To characterize the alpha-fetoprotein （AFP） positive and negative hepatocellular carcinoma （HCC） samples. METHODS： Thirty-seven paraffin-embedded human HCC samples were analyzed by immunohistochemistry for ...AIM： To characterize the alpha-fetoprotein （AFP） positive and negative hepatocellular carcinoma （HCC） samples. METHODS： Thirty-seven paraffin-embedded human HCC samples were analyzed by immunohistochemistry for the following antigens： AFP,β-catenin, p53, CD44, MSH-2, MLH-1, and HNF-4. The tumors were divided into two groups based on the AFP expression. The immunophenotypic data and important clinical parameters were studied between the two groups. RESULTS： Twenty-one of the thirty-seven examined HCCs were AFP positive. Seven with nudear p53 staining were AFP positive, while seven tumors with nuclear β-catenin staining were AFP negative. CD44 staining and high histological tumor grade were more frequent among the AFP-positive HCCs. The other immunophenotypical and dinical parameters did not show statistically significant difference in their distribution between the AFP positive and negative samples. CONCLUSION： AFP expression in HCC correlates with unfavorable prognostic factors, while nuclear β-catenin positivity is more common among the AFP-negative liver tumors. This observation supports the microarray data on in vivo human tumors.展开更多
This paper focuses on the dynamic control of distillation column with side reactors(SRC) for methyl acetate production. To obtain the optimum integrated structure and steady state simulation, the systematic design app...This paper focuses on the dynamic control of distillation column with side reactors(SRC) for methyl acetate production. To obtain the optimum integrated structure and steady state simulation, the systematic design approach based on the concept of independent reaction amount is applied to the process of SRC for methyl acetate production. In addition to the basic control loops, multi-variable model predictive control modular with methyl acetate concentration and temperature of sensitive plate is designed. Then, based on process simulation software Aspen Plus, dynamic simulation of SRC for methyl acetate production is used to verify the effectiveness of the control scheme.展开更多
With the rapid growth of the offshore wind industry, the innovative floating offshore wind turbine is chosen as the most feasible device to harvest the vast wind energy in deep water area. However there is no practica...With the rapid growth of the offshore wind industry, the innovative floating offshore wind turbine is chosen as the most feasible device to harvest the vast wind energy in deep water area. However there is no practical design guide for the floating wind turbine especially the floating foundation. In this paper, based on the investigation on the worldwide floating wind turbine and current available expertise on floating platforms accumulated in offshore O/G （oil and gas） industry, an integrated design methodology is presented according to the specialized characteristics of wind turbine, including the type selection of foundation and mooring system, design standard, design procedure, design conditions, key technologies involved. Finally a semi-submersible floating foundation is designed to support certain megawatt-rating wind turbine of Goldwind and also performance analysis and code checks are performed to validate the design. The design method of the floating foundation provided in this paper is proved feasible and can be adopted in practical engineering design.展开更多
文摘Objectives: To clone genes specifically expressed in the placenta of patients with preeclampsia. and to explain the mechanism in the etiopathology of preeclampsia. Methods: The placentae of preeclamptic and normotensive subjects with pregnancy were used as models, and the eDNA Library was constructed and 20 differentially expressed fragments were cloned after a new version of PCR-based subtractive hybridization. The false positive clones were identified by reverse dot blot analysis. With one of the obtained gene taken as the probe, the placentas of 10 normal pregnant women and 10 preeclamptic patients were studied by using dot hybridization methods. Results: Six false positive clones were identified by reverse dot blot, and the rest 14 clones were identified as preeclampsia-related genes. These clones were sequenced, and analyzed with BLAST analysis system. Eleven of 14 clones were genes already known, among which one belongs to necdin family; the rest 3 were identified as novel genes. These 3 genes were acknowledged by GenBank, with the accession numbers AF2322 16, AF2322 17, AF233648. The results of dot hybridization using necdin gene as probe were as follows: (1) There was this mRNA in the placental tissues of normal pregnancy as well as in that of preeclampsia. (2) The intensity of transcription of this mRNA in the placental tissues of preeclampsia increased significantly compared with that of the normal pregnancy (P＜0.05). Conclusions: This study for the first time reported this group of genes, especially necdin-expressing gene, which are related to the etiopathology of preeclampsia. In addition, the overtranscription of necdin gene has been found in preeclampsia. it is helpful in further studies of the etiology of preeclampsia.
基金Supported by the National Natural Science Foundation of China(21676299,21476261and 21606255)
文摘The dividing wall column （DWC） is considered as a major breakthrough in distillation technology and has good prospect of industrialization. Model predictive control （MPC） is an advanced control strategy that has acquired extensive applications in various industries. In this study, MPC is applied to the process for separating ethanol, n-propanol, and n-butanol ternary mixture in a fully thermally coupled DWC. Both composition control and tem- perature inferent/al control are considered. The multiobjective genetic algor/thm function ＂gamult/obj＂ in Matlab is used for the weight tuning of MPC. Comparisons are made between the control performances of MPC and PI strategies. Simulation results show that although both MPC and PI schemes can stabilize the DWC in case of feed disturbances, MPC generally behaves better than the PI strategy for both composition control and tempera- ture inferential control, resulting in a more stable and superior performance with lower values of integral of squared error （ISE）.
基金Supported by the National Science Foundation of Hungary, No.OTKA 42674
文摘AIM： To characterize the alpha-fetoprotein （AFP） positive and negative hepatocellular carcinoma （HCC） samples. METHODS： Thirty-seven paraffin-embedded human HCC samples were analyzed by immunohistochemistry for the following antigens： AFP,β-catenin, p53, CD44, MSH-2, MLH-1, and HNF-4. The tumors were divided into two groups based on the AFP expression. The immunophenotypic data and important clinical parameters were studied between the two groups. RESULTS： Twenty-one of the thirty-seven examined HCCs were AFP positive. Seven with nudear p53 staining were AFP positive, while seven tumors with nuclear β-catenin staining were AFP negative. CD44 staining and high histological tumor grade were more frequent among the AFP-positive HCCs. The other immunophenotypical and dinical parameters did not show statistically significant difference in their distribution between the AFP positive and negative samples. CONCLUSION： AFP expression in HCC correlates with unfavorable prognostic factors, while nuclear β-catenin positivity is more common among the AFP-negative liver tumors. This observation supports the microarray data on in vivo human tumors.
基金Supported by the National Natural Science Foundation of China(61673205,61503181,21727818)National Key R&D Program of China(2017YFB0307304)+1 种基金the Natural Science Foundation of Jiangsu Province(BK20141461,BK20140953)the State Key Laboratory of Materials-Oriented Chemical Engineering Open Subject(kl16-07)
文摘This paper focuses on the dynamic control of distillation column with side reactors(SRC) for methyl acetate production. To obtain the optimum integrated structure and steady state simulation, the systematic design approach based on the concept of independent reaction amount is applied to the process of SRC for methyl acetate production. In addition to the basic control loops, multi-variable model predictive control modular with methyl acetate concentration and temperature of sensitive plate is designed. Then, based on process simulation software Aspen Plus, dynamic simulation of SRC for methyl acetate production is used to verify the effectiveness of the control scheme.
文摘With the rapid growth of the offshore wind industry, the innovative floating offshore wind turbine is chosen as the most feasible device to harvest the vast wind energy in deep water area. However there is no practical design guide for the floating wind turbine especially the floating foundation. In this paper, based on the investigation on the worldwide floating wind turbine and current available expertise on floating platforms accumulated in offshore O/G （oil and gas） industry, an integrated design methodology is presented according to the specialized characteristics of wind turbine, including the type selection of foundation and mooring system, design standard, design procedure, design conditions, key technologies involved. Finally a semi-submersible floating foundation is designed to support certain megawatt-rating wind turbine of Goldwind and also performance analysis and code checks are performed to validate the design. The design method of the floating foundation provided in this paper is proved feasible and can be adopted in practical engineering design.
