We report a synthetic design and the experimental exploration of preparation of disubstituted polyacetylenes （PAs, P3） through 1,3-dipolar cycloaddition of azides with precursor PA bearing alkyne pendants. The precu...We report a synthetic design and the experimental exploration of preparation of disubstituted polyacetylenes （PAs, P3） through 1,3-dipolar cycloaddition of azides with precursor PA bearing alkyne pendants. The precursor PA （P2） was derived by desilylation of the pristine PA with trimethylethynylsilane side chains （P1）. P1 was obtained by polymerization of a dual-alkyne containing monomer with one of the alkynes end-capping by trimethylsilane （M） under the promotion of WC16-Ph4Sn catalyst. Two synthetic routes, i.e. two-steps （from P1 to P3 via precursor P2） and one-pot （from P1 to P3 without separation and purification of P2） were tried and the results indicated that one-pot strategy is more facile and resultant P3-1 showed higher purity and higher molecular weight than the resultant of P3-2. By using the techniques such as GPC, FTIR and 1H NMR spectroscopy the polymerization behavior and the structures of the polymers were well characterized.展开更多
An electrochemiluminescent (ECL) biosensor was constructed for selective assay of alanine aminotransferase (ALT) based on the enzymatically catalyzed oxidation of pyruvate by pyruvate oxidase (PYOD). The composite of ...An electrochemiluminescent (ECL) biosensor was constructed for selective assay of alanine aminotransferase (ALT) based on the enzymatically catalyzed oxidation of pyruvate by pyruvate oxidase (PYOD). The composite of potassium ferricyanide and carbon nanotube was adopted to pre-functionalize the basal platinum electrode while the potassium ferricyanide acted as the activator of PYOD. The ALT catalyzed the reaction of L-alanine and-ketoglutarate to produce pyruvate which could be further enzymatically oxidized by PYOD to yield H2O2 to intensify the ECL of luminol. The biosensor showed rapid response for real-time measurement of ALT in the linear concentration range from 0.00475 to 350 U/L (r = 0.993) with a relatively standard deviation of 2.5% (CALT = 47.5 U/L,n = 6). The biosensor was applied to assay the ALT in rat serum with average recovery of 90.5%.展开更多
Photofunctional materials with room-temperature phosphorescence（RTP）commonly appeared in expensive metal-coordination complexes and rare-earth-based compounds.Recently,the metal-free organic RTP materials have been ...Photofunctional materials with room-temperature phosphorescence（RTP）commonly appeared in expensive metal-coordination complexes and rare-earth-based compounds.Recently,the metal-free organic RTP materials have been paid growing attention from scientific community because of the ease of molecular design,low cost as well as potential applications in molecular switches,chemical sensors and biological imaging.To date,efficient RTP materials with high quantum yield are still very limited due to the T_1-S_0 spinforbidden process and weak spin-orbital coupling.Current mechanism based on crystallization-induced or aggregationinduced phosphorescence may serve as an effective way to enhance the RTP[1,2];展开更多
基金partially supported by the National Natural Science Foundation of China (21074113,20634020 & 20974028)the National Basic Research Program of China (973 Program,2009CB623605)+1 种基金the Research Grants Council of Hong Kong (603509 & HKUST2/CRF/10)the University Grants Committee of Hong Kong (AoE/P-03/08)
文摘We report a synthetic design and the experimental exploration of preparation of disubstituted polyacetylenes （PAs, P3） through 1,3-dipolar cycloaddition of azides with precursor PA bearing alkyne pendants. The precursor PA （P2） was derived by desilylation of the pristine PA with trimethylethynylsilane side chains （P1）. P1 was obtained by polymerization of a dual-alkyne containing monomer with one of the alkynes end-capping by trimethylsilane （M） under the promotion of WC16-Ph4Sn catalyst. Two synthetic routes, i.e. two-steps （from P1 to P3 via precursor P2） and one-pot （from P1 to P3 without separation and purification of P2） were tried and the results indicated that one-pot strategy is more facile and resultant P3-1 showed higher purity and higher molecular weight than the resultant of P3-2. By using the techniques such as GPC, FTIR and 1H NMR spectroscopy the polymerization behavior and the structures of the polymers were well characterized.
基金supported by the National Natural Science Foundation of China (20275025 & 20675055)the Natural Science Fundation of Jiangsu Province (BK2009111)Technology Plan of Suzhou (SYJG0901)
文摘An electrochemiluminescent (ECL) biosensor was constructed for selective assay of alanine aminotransferase (ALT) based on the enzymatically catalyzed oxidation of pyruvate by pyruvate oxidase (PYOD). The composite of potassium ferricyanide and carbon nanotube was adopted to pre-functionalize the basal platinum electrode while the potassium ferricyanide acted as the activator of PYOD. The ALT catalyzed the reaction of L-alanine and-ketoglutarate to produce pyruvate which could be further enzymatically oxidized by PYOD to yield H2O2 to intensify the ECL of luminol. The biosensor showed rapid response for real-time measurement of ALT in the linear concentration range from 0.00475 to 350 U/L (r = 0.993) with a relatively standard deviation of 2.5% (CALT = 47.5 U/L,n = 6). The biosensor was applied to assay the ALT in rat serum with average recovery of 90.5%.
文摘Photofunctional materials with room-temperature phosphorescence（RTP）commonly appeared in expensive metal-coordination complexes and rare-earth-based compounds.Recently,the metal-free organic RTP materials have been paid growing attention from scientific community because of the ease of molecular design,low cost as well as potential applications in molecular switches,chemical sensors and biological imaging.To date,efficient RTP materials with high quantum yield are still very limited due to the T_1-S_0 spinforbidden process and weak spin-orbital coupling.Current mechanism based on crystallization-induced or aggregationinduced phosphorescence may serve as an effective way to enhance the RTP[1,2];
