The realization of real-time thermal feedback for monitoring photothermal therapy(PTT)under near-infrared(NIR)light irradiation is of great interest and challenge for antitumor therapy.Herein,by assembling highly effi...The realization of real-time thermal feedback for monitoring photothermal therapy(PTT)under near-infrared(NIR)light irradiation is of great interest and challenge for antitumor therapy.Herein,by assembling highly efficient photothermal conversion gold nanorods and a temperature-responsive probe((E)-4-(4-(diethylamino)styryl)-1-methylpyridin-1-ium,PyS)within MOF-199,an intelligent nanoplatform(AMPP)was fabricated for simultaneous chemodynamic therapy and NIR light-induced temperature-feedback PTT.The fluorescence intensity and temperature of the PyS probe are linearly related due to the restriction of the rotation of the characteristic monomethine bridge.Moreover,the copper ions resulting from the degradation of MOF-199 in an acidic microenvironment can convert H_(2)O_(2)into•OH,resulting in tumor ablation through a Fenton-like reaction,and this process can be accelerated by increasing the temperature.This study establishes a feasible platform for fabricating highly sensitive temperature sensors for efficient temperature-feedback PTT.展开更多
Objective To investigate the optimal dosage of pirfenidone for the treatment of pulmonary fibrosis induced by bleomycin in Wistar rats, and the alteration of expressions of transforming growth factor beta-1 （ TGF-β1...Objective To investigate the optimal dosage of pirfenidone for the treatment of pulmonary fibrosis induced by bleomycin in Wistar rats, and the alteration of expressions of transforming growth factor beta-1 （ TGF-β1 ）, tissue inhibitor of metalloproteinase-1 （ TIMP-1 ）, and matrix metalloproteinase-13 （ MMP-13 ） in lung tissue. Methods Male Wistar rats were endotracheally instilled with bleomycin or normal saline. Pirfenidone （25-800 mg · kg^-l · d^-1 ）, dexamethasone （3 mg/kg）, or 1% carboxymethylcellulose sodium were given daily by feed 2 days before instillation of bleomycin. Groups T7 and T14 were fed pirfenidone 50 mg · kg^-1 · d^-1 at 7 days or 14 daYs after bleomycin instillation. Lungs were harvested at 28 days after bleomycin instillation. Patholological changes in luffg tissues were evaluated with HE staining. Lung collagen was stained by sirius red and measured by content of hydroxypro- line. Expression of proteins of TGF-β1 TIMP-1, and MMP-13 were detected by Western blotting. Results At doses of 25, 50, and 100 mg· kg^- 1 · d ^- 1, pirfenidone had significant anti-fibrotic effects for bleomy- cin-induced rat pulmonary fibrosis, and these effects were most significantly attenuated at the dosage of 50 mg · kg^-1 ·d^ -1（ HE： P 〈 0. 01, P 〈 0.01, and P = 0.064; sirius red： P 〈0.05, P 〈 0.01, and P 〈 0.05 ; hydroxyproline： P = 0.595, P 〈 0.01, and P = 0.976）. Pirfenidone at a dosage of 50 mg · kg^- l · d^-1 inhibited protein expression of TGF-131 and TIMP-1 in lung tissue in the early phase （0.79 and 0.75 times of control group）, but had no effect on ex- nr^eelnn nf MMP-13. Conclusion Low dose pirfenidone, especially at dosage of 50 mg · kg^-1 · d^-1, has significant anti-fibrotic effects on bleomycin-induced rat pulmonary fibrosis. Pirfenidone partially inhibits the enhancement of the expression of TGF-131 and TIMP-β1 in lung tissue.展开更多
Objective:The aim of this study was to study changes of HER-2 expression after neoadjuvant chemotherapy in the breast cancer cases.Methods:One hundred and thirty-seven female patients with primary breast cancers,who r...Objective:The aim of this study was to study changes of HER-2 expression after neoadjuvant chemotherapy in the breast cancer cases.Methods:One hundred and thirty-seven female patients with primary breast cancers,who received neoadjuvant chemotherapy,underwent core needle puncture and Mammotome biopsy before chemotherapy,and the biopsy results were used as the basis of histological diagnosis,fluorescence in situ hybridization (FISH) was performed to test HER2 status of tumor tissues before and after chemotherapy.All patients underwent FEC,TE,or AC neoadjuvant chemotherapy of 2-6 cycles before surgery.Results:Twenty-two patients were positive according to FISH test among 137 preoperative patients,8 patients achieved pathological complete remission after chemotherapy (three HER-2 positive patients and five negative patients),91 patients achieved partial remission,24 patients were stable,and 14 cases were invalid.Twenty-two patients were positive according to FISH test (8 patients with pathological complete remission did not undergo test),and positive patients still expressed positively after chemotherapy before neoadjuvant chemotherapy.Three negative patients were converted to be positive,and changes before and after chemotherapy had no statistical difference (P>0.05).Conclusion:Neoadjuvant chemotherapy makes no influence on patients with HER-2 positive expression,while patients with negative expression can be converted to be positive,but without significant difference.展开更多
Bi-metallic(Pt–Sn and Sn–Ni) and tri-metallic(Pt–Sn–Ni) catalysts,supported on Al-containing hexagonal mesoporous silica(Al-HMS)(Si/Al = 20) materials,were synthesized.N_2 adsorption–desorption,X-ray diffraction(...Bi-metallic(Pt–Sn and Sn–Ni) and tri-metallic(Pt–Sn–Ni) catalysts,supported on Al-containing hexagonal mesoporous silica(Al-HMS)(Si/Al = 20) materials,were synthesized.N_2 adsorption–desorption,X-ray diffraction(XRD),Brunauer–Emmett–Teller(BET) test,and temperature programed desorption(NH3-TPD)were used to characterize physicochemical characteristics and textural properties of the Al-HMS catalysts.Catalytic performances on hydro-cracking of n-decane at different reaction conditions were studied in a microreactor.Comparison between Pt–Sn,Sn–Ni and Pt–Sn–Ni catalyst under different hydro-cracking conditions was made.The experimental results indicate that the proper balance between the acid and metal functions is the key in synthesizing a catalyst with a better performance in hydro-cracking.Tri-metallic catalyst exhibits the best catalytic performance in n-decane hydro-cracking than two bi-metallic catalysts.展开更多
Objective: To observe the effects of Chinese decoction and ligustrazin hydrochloride injection combined with He-Ne laser on lipoperoxide (LPO) and Superoxide dismutase (SOD) in patients with chloasma. Methods: 90 case...Objective: To observe the effects of Chinese decoction and ligustrazin hydrochloride injection combined with He-Ne laser on lipoperoxide (LPO) and Superoxide dismutase (SOD) in patients with chloasma. Methods: 90 cases of chloasma were randomly divided into the following two groups: a treatment group (of 54 cases) treated by a self-prepared prescription for toning the kidneys and relieving the depressed liver to remove blood stasis, ligustrazin hydrochloricde injection and He-Ne laser therapy, and a control group (of 36 cases) treated with oral administration of Vitamin E and Vitamin C plus external application of 20% Azelaic acid cream. Results: The total effective rate in the treatment group was 79.6%, which was significantly higher than that of the control group (P<0.05). After treatment, the LPO level in the treatment group was significantly lowered (P<0.01), and the SOD level was significantly elevated (P<0.05). Conclusion: The therapeutic methods adopted in the treatment group may show the action of antioxidation, providing good clinical effects for treating chloasma.展开更多
Hypoxia is a hallmark of solid tumors,and it significantly impairs the overall anticancer efficacy,particularly photodynamic therapy(PDT).Herein,a catalase-like nanovesicle with near-infrared light-responsiveness,that...Hypoxia is a hallmark of solid tumors,and it significantly impairs the overall anticancer efficacy,particularly photodynamic therapy(PDT).Herein,a catalase-like nanovesicle with near-infrared light-responsiveness,that is,platinum/gold nanoshell encapsulated chlorin e6(Ce6)/resveratrol(Res)liposome(Pt@Au-Ce6/Res-Lip),was developed to surmount this intractable issue.The Pt@Au-Ce6/Res-Lip can decompose overexpressed H_(2)O_(2)in tumor microenvironment to produce vast amounts of O_(2)for further enhancement of the PDT.Under the 808 nm laser irradiation,the Au nanoshells induced hyperthermia at the lesion site to ablate tumor cells,simultaneously inducing the controlled release of photosensitizer Ce6 and chemotherapeutic agent Res.Moreover,stimulated by 660 nm laser,numerous reactive oxygen species were formed to induce apoptosis and necrosis of tumor cells.With the cascade of trimodal therapeutic modality options(chemotherapy,photothermal therapy,and PDT),the Pt@Au-Ce6/Res-Lip showed ultrahigh tumor inhabitation rate in in vitro and in vivo studies,signifying that the Pt@AuCe6/Res-Lip nanovesicle is a promising candidate for effective cancer therapy.展开更多
The effect of tumor-targeted photodynamic therapy（PDT） was improved by designing nanotheranostics to promote oxygenation in a tumor microenvironment（TME）wherein hypoxia, acidosis, and the elevated levels of H2O2 a...The effect of tumor-targeted photodynamic therapy（PDT） was improved by designing nanotheranostics to promote oxygenation in a tumor microenvironment（TME）wherein hypoxia, acidosis, and the elevated levels of H2O2 are three main characteristics. In this study, a carbon dot（CD）PDT agent recently developed by our group was firstly applied as reducing agent to react with potassium permanganate for fabricating CDs/manganese dioxide（CDs/MnO2） composites,which were in turn modified with polyethylene glycol（PEG） to form water-soluble CDs/MnO2-PEG nanohybrids. In a normal physiological environment, the as-prepared nanohybrids exhibited quenched fluorescence, weak singlet oxygen generation, and low magnetic resonance imaging（MRI） signal.However, given the high sensitivity of MnO2 to the TME, the CDs/MnO2-PEG nanohybrids changed from an ＂off＂ to an＂on＂ state with synchronously enhanced fluorescence, singlet oxygen generation, and MRI signal in the TME. In vitro and in vivo analyses have revealed that CDs/MnO2-PEG nanohybrids could be applied as TME-driven, turn-on nanotheranostics for the MR/fluorescence bimodal imaging-guided PDT of cancer.Moreover, complete clearance of CDs/MnO2-PEG nanohybrids from the body of mice was observed, indicating their low long-term toxicity and good biocompatibility. This work offers a new nanotheranostic candidate for modulating the unfavorable TME, particularly for the targeted PDT of cancer through precise positioning and oxygen generation.展开更多
Photocurable systems, which offer advantages such as microfabrication and in situ fabrication, have been widely used as dental restorative materials. Because the visible light-curable(VLC) system causes no biological ...Photocurable systems, which offer advantages such as microfabrication and in situ fabrication, have been widely used as dental restorative materials. Because the visible light-curable(VLC) system causes no biological damage, it is popular as a dental material and is being investigated by many researchers for other medical applications. Here, the principle of the VLC system is explained and recent progress in key components including photoinitiators, monomers, macromers, and prepolymers is discussed. Finally, biomedical applications for drug delivery and soft tissue engineering are reviewed. Considering the recent development of VLC systems, its importance in the field of medical applications is expected to continue to increase in the future.展开更多
The abuse of antibiotics in treating microbial infections has led to the emergence and prevalence of drugresistant bacteria.Thus,the development of novel antibacterial materials is attracting increasing attention.Here...The abuse of antibiotics in treating microbial infections has led to the emergence and prevalence of drugresistant bacteria.Thus,the development of novel antibacterial materials is attracting increasing attention.Here,a series of flexible electrostatic hydrogels with excellent antibacterial ability were constructed using a mixture of nitric oxide(NO)-releasing nitrated chitosan(CSNO)and mesotetra(4-carboxyphenyl)porphine(TCPP)with salmon sperm DNA(ssDNA)solution.When cultured with gram-negative bacteria under solar simulator irradiation,TCPP-CSNO_(m)ssDNA_(n) hydrogels released reactive oxygen species(ROS)and NO to produce peroxynitrite ions(ONOO^(−)).ONOO−is efficient at killing bacteria,thereby improving the antimicrobial ability of photodynamic therapy against gram-negative bacteria.The hydrogels exhibited powerful antibacterial activity in vivo when used to treat skin infections caused by drugresistant bacteria,making them a promising candidate for clinical applications.A string of antibacterial hydrogels that release ROS and NO synergistically can bring new possibilities for effectively killing drug-resistant bacteria and be of great value in anti-infection wound dressings and other applications.展开更多
A custom built 1310 nm center wavelength swept source optical coherence tomography instrument was used to measure morphological changes in treated and newly diagnosed untreated glaucomatous human Schlemm’s canal(SC)....A custom built 1310 nm center wavelength swept source optical coherence tomography instrument was used to measure morphological changes in treated and newly diagnosed untreated glaucomatous human Schlemm’s canal(SC).Thirty-seven primary open-angle glaucoma patients were divided into two groups depending on the patients having been treated or not.The statistical results showed that there were significant differences between the treated and untreated groups’SC areas(treated,7935.6875±680.003μm2;untreated,3890.71875±871.49844μm2;P<0.001),the circumferences(treated,580.37891±44.96529μm;untreated,381.9026±41.22123μm;P<0.001),and the long diameters(treated,272.87806±25.7254μm;untreated,185.24047±19.72786μm;P<0.001).We hypothesize that,after drug treatment,the SC will expand and the morphometric values especially the areas will become larger,thus helping to reduce intraocular pressure.展开更多
Utilizing the unique tumor microenvironment(TME)to conduct chemical reactions for cancer treatment becomes a hot topic recently.Nevertheless,single chemical reaction in TME is often restricted by scanty reaction subst...Utilizing the unique tumor microenvironment(TME)to conduct chemical reactions for cancer treatment becomes a hot topic recently.Nevertheless,single chemical reaction in TME is often restricted by scanty reaction substrates and slow reaction rate.Meanwhile,the toxic substances produced by the reactions are usually not enough to kill cancer cells.Herein,using covalent organic frameworks(COFs)as the template,Au nanoparticles(Au NPs)were subsequently grown on the surface of the COF,then a thin layer of manganese dioxide(MnO2)was coated over the material,and finally hyaluronic acid(HA)was introduced to improve the biocompatibility.The resultant product,named COF-Au-MnO2,was involved in several processes to form cascade reactions in the TME.Specifically,under hypoxic conditions,COF-Au-MnO2 could react with intratumoral H2O2 to produce O2 to enhance the type II photodynamic therapy(PDT),and Au NPs could decompose glucose to promote starving-like therapy.Besides,starving-like therapy can also produce H2O2 to increase O2 production.Simultaneously,MnO2 can consume glutathione(GSH)to enhance the antitumor efficacy,and the released Mn2+could be used for T1-weighted magnetic resonance imaging(MRI).Both in vitro and in vivo experiments had proven excellent cancer cell killing effect and antitumor efficacy of COF-Au-MnO2via such a cycle-like process.展开更多
Molybdenum oxide nanoparticles(NPs) with tunable plasmonic resonance in the near-infrared region display superior semiconducting features and photothermal properties, which are highly related to the crystalline and de...Molybdenum oxide nanoparticles(NPs) with tunable plasmonic resonance in the near-infrared region display superior semiconducting features and photothermal properties, which are highly related to the crystalline and defective structures such as oxygen deficiencies. However,fundamental understanding on the structure-function relationship between crystalline/defective structures and photothermal properties is still unclear. To address this, herein,we have developed an "in-situ confined oxidation-reduction"strategy to regulate the defect features of molybdenum oxide NPs in the dual-mesoporous silica nanoreactor. Especially, the effects of crystalline structure/oxygen defects of molybdenum oxides on the photothermal performances were investigated by facilely tuning the amount of molybdenum resource and the reduction temperature. As a photothermal nanoagent, the optimal defective molybdenum oxide NPs encapsulated in PEGylated porous silica nanoreactor(designated as MoO_(3)@PPSNs) exhibit excellent biological stability and strong localized surface plasmon resonance effect in nearinfrared absorption range with the highest photothermal conversion efficiency up to 78.7% under 808 nm laser irradiation. More importantly, the remarkable photothermal effects of MoO_(3)@PPSNs were comprehensively demonstrated both in vitro and in vivo. Consequently, we envision that the plasmonic MoO_(3)NPs in a biocompatible porous silica nanoreactor could be used as an efficient photothermal therapy agent for photothermal ablation of tumors.展开更多
基金supported by the National Natural Science Foundation of China(22171001,22305001,51972001,52372073)the Natural Science Foundation of Anhui Province of China(2108085MB49).
文摘The realization of real-time thermal feedback for monitoring photothermal therapy(PTT)under near-infrared(NIR)light irradiation is of great interest and challenge for antitumor therapy.Herein,by assembling highly efficient photothermal conversion gold nanorods and a temperature-responsive probe((E)-4-(4-(diethylamino)styryl)-1-methylpyridin-1-ium,PyS)within MOF-199,an intelligent nanoplatform(AMPP)was fabricated for simultaneous chemodynamic therapy and NIR light-induced temperature-feedback PTT.The fluorescence intensity and temperature of the PyS probe are linearly related due to the restriction of the rotation of the characteristic monomethine bridge.Moreover,the copper ions resulting from the degradation of MOF-199 in an acidic microenvironment can convert H_(2)O_(2)into•OH,resulting in tumor ablation through a Fenton-like reaction,and this process can be accelerated by increasing the temperature.This study establishes a feasible platform for fabricating highly sensitive temperature sensors for efficient temperature-feedback PTT.
基金Supported by National Ministry of Education Doctor Foundation of China(20020023045)
文摘Objective To investigate the optimal dosage of pirfenidone for the treatment of pulmonary fibrosis induced by bleomycin in Wistar rats, and the alteration of expressions of transforming growth factor beta-1 （ TGF-β1 ）, tissue inhibitor of metalloproteinase-1 （ TIMP-1 ）, and matrix metalloproteinase-13 （ MMP-13 ） in lung tissue. Methods Male Wistar rats were endotracheally instilled with bleomycin or normal saline. Pirfenidone （25-800 mg · kg^-l · d^-1 ）, dexamethasone （3 mg/kg）, or 1% carboxymethylcellulose sodium were given daily by feed 2 days before instillation of bleomycin. Groups T7 and T14 were fed pirfenidone 50 mg · kg^-1 · d^-1 at 7 days or 14 daYs after bleomycin instillation. Lungs were harvested at 28 days after bleomycin instillation. Patholological changes in luffg tissues were evaluated with HE staining. Lung collagen was stained by sirius red and measured by content of hydroxypro- line. Expression of proteins of TGF-β1 TIMP-1, and MMP-13 were detected by Western blotting. Results At doses of 25, 50, and 100 mg· kg^- 1 · d ^- 1, pirfenidone had significant anti-fibrotic effects for bleomy- cin-induced rat pulmonary fibrosis, and these effects were most significantly attenuated at the dosage of 50 mg · kg^-1 ·d^ -1（ HE： P 〈 0. 01, P 〈 0.01, and P = 0.064; sirius red： P 〈0.05, P 〈 0.01, and P 〈 0.05 ; hydroxyproline： P = 0.595, P 〈 0.01, and P = 0.976）. Pirfenidone at a dosage of 50 mg · kg^- l · d^-1 inhibited protein expression of TGF-131 and TIMP-1 in lung tissue in the early phase （0.79 and 0.75 times of control group）, but had no effect on ex- nr^eelnn nf MMP-13. Conclusion Low dose pirfenidone, especially at dosage of 50 mg · kg^-1 · d^-1, has significant anti-fibrotic effects on bleomycin-induced rat pulmonary fibrosis. Pirfenidone partially inhibits the enhancement of the expression of TGF-131 and TIMP-β1 in lung tissue.
文摘Objective:The aim of this study was to study changes of HER-2 expression after neoadjuvant chemotherapy in the breast cancer cases.Methods:One hundred and thirty-seven female patients with primary breast cancers,who received neoadjuvant chemotherapy,underwent core needle puncture and Mammotome biopsy before chemotherapy,and the biopsy results were used as the basis of histological diagnosis,fluorescence in situ hybridization (FISH) was performed to test HER2 status of tumor tissues before and after chemotherapy.All patients underwent FEC,TE,or AC neoadjuvant chemotherapy of 2-6 cycles before surgery.Results:Twenty-two patients were positive according to FISH test among 137 preoperative patients,8 patients achieved pathological complete remission after chemotherapy (three HER-2 positive patients and five negative patients),91 patients achieved partial remission,24 patients were stable,and 14 cases were invalid.Twenty-two patients were positive according to FISH test (8 patients with pathological complete remission did not undergo test),and positive patients still expressed positively after chemotherapy before neoadjuvant chemotherapy.Three negative patients were converted to be positive,and changes before and after chemotherapy had no statistical difference (P>0.05).Conclusion:Neoadjuvant chemotherapy makes no influence on patients with HER-2 positive expression,while patients with negative expression can be converted to be positive,but without significant difference.
文摘Bi-metallic(Pt–Sn and Sn–Ni) and tri-metallic(Pt–Sn–Ni) catalysts,supported on Al-containing hexagonal mesoporous silica(Al-HMS)(Si/Al = 20) materials,were synthesized.N_2 adsorption–desorption,X-ray diffraction(XRD),Brunauer–Emmett–Teller(BET) test,and temperature programed desorption(NH3-TPD)were used to characterize physicochemical characteristics and textural properties of the Al-HMS catalysts.Catalytic performances on hydro-cracking of n-decane at different reaction conditions were studied in a microreactor.Comparison between Pt–Sn,Sn–Ni and Pt–Sn–Ni catalyst under different hydro-cracking conditions was made.The experimental results indicate that the proper balance between the acid and metal functions is the key in synthesizing a catalyst with a better performance in hydro-cracking.Tri-metallic catalyst exhibits the best catalytic performance in n-decane hydro-cracking than two bi-metallic catalysts.
文摘Objective: To observe the effects of Chinese decoction and ligustrazin hydrochloride injection combined with He-Ne laser on lipoperoxide (LPO) and Superoxide dismutase (SOD) in patients with chloasma. Methods: 90 cases of chloasma were randomly divided into the following two groups: a treatment group (of 54 cases) treated by a self-prepared prescription for toning the kidneys and relieving the depressed liver to remove blood stasis, ligustrazin hydrochloricde injection and He-Ne laser therapy, and a control group (of 36 cases) treated with oral administration of Vitamin E and Vitamin C plus external application of 20% Azelaic acid cream. Results: The total effective rate in the treatment group was 79.6%, which was significantly higher than that of the control group (P<0.05). After treatment, the LPO level in the treatment group was significantly lowered (P<0.01), and the SOD level was significantly elevated (P<0.05). Conclusion: The therapeutic methods adopted in the treatment group may show the action of antioxidation, providing good clinical effects for treating chloasma.
基金the National Natural Science Foundation(21776238,21476190,and 31801198)Hebei province Natural fund key projects(B2019203479)。
文摘Hypoxia is a hallmark of solid tumors,and it significantly impairs the overall anticancer efficacy,particularly photodynamic therapy(PDT).Herein,a catalase-like nanovesicle with near-infrared light-responsiveness,that is,platinum/gold nanoshell encapsulated chlorin e6(Ce6)/resveratrol(Res)liposome(Pt@Au-Ce6/Res-Lip),was developed to surmount this intractable issue.The Pt@Au-Ce6/Res-Lip can decompose overexpressed H_(2)O_(2)in tumor microenvironment to produce vast amounts of O_(2)for further enhancement of the PDT.Under the 808 nm laser irradiation,the Au nanoshells induced hyperthermia at the lesion site to ablate tumor cells,simultaneously inducing the controlled release of photosensitizer Ce6 and chemotherapeutic agent Res.Moreover,stimulated by 660 nm laser,numerous reactive oxygen species were formed to induce apoptosis and necrosis of tumor cells.With the cascade of trimodal therapeutic modality options(chemotherapy,photothermal therapy,and PDT),the Pt@Au-Ce6/Res-Lip showed ultrahigh tumor inhabitation rate in in vitro and in vivo studies,signifying that the Pt@AuCe6/Res-Lip nanovesicle is a promising candidate for effective cancer therapy.
基金supported by the National Natural Science Foundation of China (51472252 and 51572269)the Strategic Priority Research Program of the Chinese Academy of Sciences (XDB17000000)
文摘The effect of tumor-targeted photodynamic therapy（PDT） was improved by designing nanotheranostics to promote oxygenation in a tumor microenvironment（TME）wherein hypoxia, acidosis, and the elevated levels of H2O2 are three main characteristics. In this study, a carbon dot（CD）PDT agent recently developed by our group was firstly applied as reducing agent to react with potassium permanganate for fabricating CDs/manganese dioxide（CDs/MnO2） composites,which were in turn modified with polyethylene glycol（PEG） to form water-soluble CDs/MnO2-PEG nanohybrids. In a normal physiological environment, the as-prepared nanohybrids exhibited quenched fluorescence, weak singlet oxygen generation, and low magnetic resonance imaging（MRI） signal.However, given the high sensitivity of MnO2 to the TME, the CDs/MnO2-PEG nanohybrids changed from an ＂off＂ to an＂on＂ state with synchronously enhanced fluorescence, singlet oxygen generation, and MRI signal in the TME. In vitro and in vivo analyses have revealed that CDs/MnO2-PEG nanohybrids could be applied as TME-driven, turn-on nanotheranostics for the MR/fluorescence bimodal imaging-guided PDT of cancer.Moreover, complete clearance of CDs/MnO2-PEG nanohybrids from the body of mice was observed, indicating their low long-term toxicity and good biocompatibility. This work offers a new nanotheranostic candidate for modulating the unfavorable TME, particularly for the targeted PDT of cancer through precise positioning and oxygen generation.
基金supported by the Foreign Postdoctoral Researcher program of RIKEN and JSPS KAKENHI(22220009)
文摘Photocurable systems, which offer advantages such as microfabrication and in situ fabrication, have been widely used as dental restorative materials. Because the visible light-curable(VLC) system causes no biological damage, it is popular as a dental material and is being investigated by many researchers for other medical applications. Here, the principle of the VLC system is explained and recent progress in key components including photoinitiators, monomers, macromers, and prepolymers is discussed. Finally, biomedical applications for drug delivery and soft tissue engineering are reviewed. Considering the recent development of VLC systems, its importance in the field of medical applications is expected to continue to increase in the future.
基金supported by the National Key R&D Program of China(2021YFB3800900)the National Natural Science Foundation of China(22122501,21875014 and 52073013)Beijing Outstanding Young Scientist Program(BJJWZYJH01201910010024)。
文摘The abuse of antibiotics in treating microbial infections has led to the emergence and prevalence of drugresistant bacteria.Thus,the development of novel antibacterial materials is attracting increasing attention.Here,a series of flexible electrostatic hydrogels with excellent antibacterial ability were constructed using a mixture of nitric oxide(NO)-releasing nitrated chitosan(CSNO)and mesotetra(4-carboxyphenyl)porphine(TCPP)with salmon sperm DNA(ssDNA)solution.When cultured with gram-negative bacteria under solar simulator irradiation,TCPP-CSNO_(m)ssDNA_(n) hydrogels released reactive oxygen species(ROS)and NO to produce peroxynitrite ions(ONOO^(−)).ONOO−is efficient at killing bacteria,thereby improving the antimicrobial ability of photodynamic therapy against gram-negative bacteria.The hydrogels exhibited powerful antibacterial activity in vivo when used to treat skin infections caused by drugresistant bacteria,making them a promising candidate for clinical applications.A string of antibacterial hydrogels that release ROS and NO synergistically can bring new possibilities for effectively killing drug-resistant bacteria and be of great value in anti-infection wound dressings and other applications.
基金supported by the Sichuan Youth Science&Technology Foundation(2013JQ0028)the National Natural Science Foundation of China(61108082)+1 种基金the West Light Foundation of the Chinese Academy of Sciencesthe National Major Scientific Equipment Program(2012YQ120080)
文摘A custom built 1310 nm center wavelength swept source optical coherence tomography instrument was used to measure morphological changes in treated and newly diagnosed untreated glaucomatous human Schlemm’s canal(SC).Thirty-seven primary open-angle glaucoma patients were divided into two groups depending on the patients having been treated or not.The statistical results showed that there were significant differences between the treated and untreated groups’SC areas(treated,7935.6875±680.003μm2;untreated,3890.71875±871.49844μm2;P<0.001),the circumferences(treated,580.37891±44.96529μm;untreated,381.9026±41.22123μm;P<0.001),and the long diameters(treated,272.87806±25.7254μm;untreated,185.24047±19.72786μm;P<0.001).We hypothesize that,after drug treatment,the SC will expand and the morphometric values especially the areas will become larger,thus helping to reduce intraocular pressure.
基金the National Natural Science Foundation of China(21471145)the Science and Technology Development Planning Project of Jilin Province(20170101179JC)the“Hundred Talents Program”of Chinese Academy of Sciences。
文摘Utilizing the unique tumor microenvironment(TME)to conduct chemical reactions for cancer treatment becomes a hot topic recently.Nevertheless,single chemical reaction in TME is often restricted by scanty reaction substrates and slow reaction rate.Meanwhile,the toxic substances produced by the reactions are usually not enough to kill cancer cells.Herein,using covalent organic frameworks(COFs)as the template,Au nanoparticles(Au NPs)were subsequently grown on the surface of the COF,then a thin layer of manganese dioxide(MnO2)was coated over the material,and finally hyaluronic acid(HA)was introduced to improve the biocompatibility.The resultant product,named COF-Au-MnO2,was involved in several processes to form cascade reactions in the TME.Specifically,under hypoxic conditions,COF-Au-MnO2 could react with intratumoral H2O2 to produce O2 to enhance the type II photodynamic therapy(PDT),and Au NPs could decompose glucose to promote starving-like therapy.Besides,starving-like therapy can also produce H2O2 to increase O2 production.Simultaneously,MnO2 can consume glutathione(GSH)to enhance the antitumor efficacy,and the released Mn2+could be used for T1-weighted magnetic resonance imaging(MRI).Both in vitro and in vivo experiments had proven excellent cancer cell killing effect and antitumor efficacy of COF-Au-MnO2via such a cycle-like process.
基金supported by the National Key Research and Development Program of China (2016YFA0203700)the National Natural Science Foundation of China (51672083, 51962022 and 52072124)+4 种基金the Natural Science Foundation of Shanghai (20ZR1414900)the Program of Shanghai Academic/Technology Research Leader (18XD1401400)the Leading Talents in Shanghai in 2018the Program for Professor of Special Appointment (Eastern Scholar) at Shanghai Institutions of Higher Learningthe 111 Project (B14018)。
文摘Molybdenum oxide nanoparticles(NPs) with tunable plasmonic resonance in the near-infrared region display superior semiconducting features and photothermal properties, which are highly related to the crystalline and defective structures such as oxygen deficiencies. However,fundamental understanding on the structure-function relationship between crystalline/defective structures and photothermal properties is still unclear. To address this, herein,we have developed an "in-situ confined oxidation-reduction"strategy to regulate the defect features of molybdenum oxide NPs in the dual-mesoporous silica nanoreactor. Especially, the effects of crystalline structure/oxygen defects of molybdenum oxides on the photothermal performances were investigated by facilely tuning the amount of molybdenum resource and the reduction temperature. As a photothermal nanoagent, the optimal defective molybdenum oxide NPs encapsulated in PEGylated porous silica nanoreactor(designated as MoO_(3)@PPSNs) exhibit excellent biological stability and strong localized surface plasmon resonance effect in nearinfrared absorption range with the highest photothermal conversion efficiency up to 78.7% under 808 nm laser irradiation. More importantly, the remarkable photothermal effects of MoO_(3)@PPSNs were comprehensively demonstrated both in vitro and in vivo. Consequently, we envision that the plasmonic MoO_(3)NPs in a biocompatible porous silica nanoreactor could be used as an efficient photothermal therapy agent for photothermal ablation of tumors.
